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Perubahan Komposisi Kimia Kulit Buah Kakao Akibat Penambahan Mangan dan Kalsium dalam Biokonversi dengan Kapang Phanerochaete chrysosporium Suparjo Suparjo; K G Wiryawan; E B Laconi; D Mangunwidjaja
Media Peternakan Vol. 32 No. 3 (2009): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (233.177 KB)

Abstract

Bioconversion is a method to increase quality of high lignocellulose-containing feedstuffs. Fermentation occurs during bioconversion is influenced primarily by length of fermentation and mineral supplementation to the medium. This study was aimed at determining the effect of these two factors on dry matter (DM), organic matter (OM), neutral detergent fiber (NDF) and acid detergent fiber (ADF), and cellulose-to-lignin ratio of cocoa pod incubated with Phanerochaete chrysosporium. Twenty four treatments containing of 4 mineral supplementations (no mineral, Ca, Mn, and Ca+Mn) and 6 different lengths of fermentation (0, 5, 10, 15, 20, and 25 days) were designed randomly to 72 fermentation glass jars in a 4x6 factorial arrangement. Length of fermentation had significant effect on all parameters measured. Mineral significantly affected changes of DM and OM, NDF and ADF content, and cellulose-to-lignin ratio, but not DM and OM content. In conclusion, supplementation of Ca to cocoa pod incubated with P. chrysosporium for 15 days contributed positively (P < 0.05) to changes of OM (13.83%) and DM (11.30%). The cellulose-to-lignin ratio of 1.34 was the optimum result of Mn supplementation for 10 days incubation. Key words: Phanerochaete chrysosporium, cocoa pod, fermentation, mineral supplemention, fiber
Performa Kambing yang Diberi Kulit Buah Kakao Terfermentasi Suparjo Suparjo; K G Wiryawan; E B Laconi; D Mangunwidjaja
Media Peternakan Vol. 34 No. 1 (2011): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (150.846 KB) | DOI: 10.5398/medpet.2011.34.1.35

Abstract

Utilization of cocoa pod husk (CPH) as feedstuff needs pretreatment to increase its nutrients availability. Bioconversion with Phanerochaete chrysosporium changes its structure by breaking down the linkage between lignin and structural carbohydrates. This experiment was aimed to evaluate the quality of fermented CPH biomass as feed for goats. The experimental treatments i.e.:  A= 30% of fresh napier grass (RG) + 50% of dried RG + 20% of concentrate; B= 30% of fresh RG + 30% of dried RG + 40% of concentrate; C= 30% of fresh RG + 30% of CPH + 40% of concentrate; D= 30% of fresh RG + 30% of fermented CPH + 40% of concentrate and E= 30% of fresh RG + 50%  of fermented CPH + 20% of concentrate. The treatments were allocated in a randomized block design with three replications.  Feed intake, body weight gain and ration efficiency were measured. The use of fermented CPH at the level of 30% had higher (P < 0.05) feed intake (560.33 g day-1), body weight gain (101.79 g head-1 day-1), and feed conversion (5.50) compared to other treatments. In conclusion that the use of 30% fermented CPH in the ration showed the best body weight gain and feed efficiency.
ISOLASI PROTEIN DAN PRODUKSI KONSENTRAT PROTEIN DAUN (KPD) SEBAGAI SUPLEMEN PAKAN TERNAK Yatno Yatno; Suparjo Suparjo; Rasmi Murni
Pastura : Jurnal Ilmu Tumbuhan Pakan Ternak Vol 7 No 2 (2018): Pastura Vol. 7 No. 2 Tahun 2018
Publisher : Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1493.158 KB) | DOI: 10.24843/Pastura.2018.v07.i02.p07

Abstract

The research aim to determine the effect of extraction using various combination of pH alkaline-acid to protein and amino acid content of Lamtoro Protein Concentrate Leaf (LPCL). Completely Randomized Design (CRD) with 3 treatments and 5 replications. The Lamtoro Leaf Meal (LLM) (50 gram) was blended in NaOH (200 ml) for 15 menit and centrifuged to separate the supernatant from extracted LLM. The extracted LLM was re-extracted with buffer acetat pH 4,6 and sentrifuged again to separate supernantant from extracted LLM. Both supernatant from NaOH and buffera acetat pH 4,6 extraction were mixed and precipitated with buffer phospat until isoelectric point. The treatments were tested consisted of extracted with NaOH pH 8, NaOH pH 9 and NaOH pH 10. The parameters measured; isoelectric point at precipited, crude protein, total amino acid (TAA), total essential amino acid (TEAA) and individual amino acid. The results showed that the precipited obtained isoelectric point pH 7 was 2.66; 1.03 and 2.58 gram at the extraction treatments using NaOH at pH 8, 9 and 10, respectively. LPCL extracted using NaOH at pH 9 and 10 contained a high crude protein of 54.82 and 52.60% higher than the extraction using NaOH at pH 8 of 46.98%. The total amount of amino acid and essential amino acid (AAE) of LPCL were highest in treatment using NaOH at pH 10 of 26,58; 13.31 %, respectively. Combination extraction LLM using pH buffer 10 followed by re-extract using acetate buffer pH 4,6 contains crude protein and good essential amino acid, especially leucine. Conclution of the research that the combination extraction using NaOH at pH 10 and followed by re-extract using acetate buffer at pH 4.6 is the best treatment contain crude protein and essential amino acid at LPCL. Keywords : Crude protein, essential amico acid, extraction.
Perbedaan Lama Waktu Perendaman Alat Tangkap Rawai (Long Line) Terhadap Hasil Tangkapan di Kecamatan Nipah Panjang Kabupaten Tanjung Jabung Timur Mustika Zahara; Nelwida Nelwida; Suparjo Suparjo; Fauzan Ramadhan; Lisna Lisna; Muhammad Hariski
Ilmu Perairan (Aquatic Science) Vol 9, No 3 (2021)
Publisher : Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jipas.9.3.p.215-221

Abstract

Tujuan dari penelitian ini adalah untuk mengetahui perbedaan lama waktu perendaman terhadap hasil tangkapan alat tangkap rawai (long line) di Kecamatan Nipah Panjang Kabupaten Tanjung Jabung Timur. Penelitian ini dilaksanakan di Kelurahan Nipah Panjang I Kabupaten Tanjung Jabung Timur pada tanggal 3 Juni 2021 sampai 30 Juni 2021. Metode yang digunakan adalah eksperimental dengan analisis data menggunakan uji t. Hasil penelitian menunjukkan bahwa perbedaan lama waktu perendaman 5 jam berpengaruh nyata (P <0,05) terhadap jumlah dan berat hasil tangkapan dibandingkan lama waktu perendaman 3 jam. Untuk jumlah pada perendaman 5 jam (113 ekor) sedangkan 3 jam (70 ekor). Untuk berat pada perendaman 5 jam (67,87 kg) sedangkan 3 jam (44,12 kg). Berdasarkan hasil penelitian dapat disimpulkan bahwa lama perendaman 5 jam terhadap hasil tangkapan dengan alat tangkap rawai lebih banyak dari lama perendaman 3 jam dengan hasil tangkapan yang didapat adalah ikan baung (Mystus nerumus), ikan sembilang (Plotosus canius), dan ikan duri (Hexanematichthys sagor).
Efektivitas Bakteri Amilolitik Asal Geopark Merangin Jambi terhadap Patogenitas Jamur Pyricularia oryzae Penyebab Penyakit Blas Daun Padi Ummi Mardhiah Batubara; Suparjo Suparjo; Hasnaul Maritsa; Nanda Fahmuin Tari; Selvi Andriani
BIO-SITE |Biologi dan Sains Terapan Vol. 3 No. 1 (2017): Bio-Site
Publisher : Biology Department, Faculty of Science and Technology, Univeristas Jambi, Indonesia

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Abstract

Rice (Oryza sativa) is a major crop product in Asia. Rice products are increasing along with the increasing of human population. Blast disease in leaf (leaf blast) is the major pathogen in rice during vegetative phase. The using of amylolytic bacteria isolated from Geopark Merangin Jambi is done as an attempt to discover biocontrol agents in order to attack infection of Pyricularia oryzae, the cause of leaf blast disease. Antimicrobial activity assay was carried out by isolating the pathogen in the infected rice crops. Pyricularia oryzae that had been cultured then were evaluated their antagonistic activity against amylolytic bacteria isolated from prior experiment. Results have shown that from 25 isolates of amylolytic bacteria taken from Geopark Merangin Jambi, 2 isolates that can be used as biocontrol agents were GM20 and GM23 capable of inhibiting the growth of Pyricularia oryzae.
PERTUMBUHAN STEK Aquilaria malaccensis Lamk. DENGAN PEMBERIAN BIOURINE SAPI Liyona Noviolla; Suparjo Suparjo
BIO-SITE |Biologi dan Sains Terapan Vol. 4 No. 2 (2018): Bio-Site
Publisher : Biology Department, Faculty of Science and Technology, Univeristas Jambi, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (555.347 KB) | DOI: 10.22437/bs.v4i2.5885

Abstract

ABSTRACT Aquilaria malaccensis is one of the agarwood-producing plants that have high economic value and has been included as a type of Appendix II by CITES, where its consequences in the formal trade,agarwood have to produced from agricultural trees instead of trees that come from nature. A.malaccensis cultivation can be done with vegetative propagation one of them by cutting. In plant propagation with cuttings required additional nutrients and natural auxin, in order to encourage roots and accelerate plant growth. One of them is by utilizing cow waste water and processed into biourine.The aim of this research was to find out the growth of A. malaccensis cuttings along with giving the biourine of cow and also the most optimal biourine concentration. The study used a completely randomized design (CRD) with 5 exercises and 3 replications. The treatment consisted of: B0 = 0% concentration biourine (without giving biourine), B1 = Biourine with a concentration of 25%, B2 = 50% biourine concentration, B3 = Biourine with a concentration of 75%, and B4 = Biourine with a concentration of 100%. The results of the study showed that biourine beef growth on A.malaccensis cuttings was seen in the parameters of plant height, leaf length, leaf width, shoot length and root length. However, it was not adequate for the number of leaves and the number of shoots. The best result for growth of A.malaccensis cuttings at75% concentrations.Keyword: Aquilaria malaccensis, RAL, Biourine
Penguatan usaha kelompok peternak pelaku integrasi sawit-sapi berbasis limbah di Kecamatan Mestong Ardi Novra; Adriani Adriani; Suparjo Suparjo; Nelson Nelson; Sri Novianti
Riau Journal of Empowerment Vol 2 No 2 (2019)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (740.155 KB) | DOI: 10.31258/raje.2.2.43-54

Abstract

This strengthening waste-based palm oil-cattle integration groups business program is a continuation of the development program in the 2013. It is intended to accelerate and increase production capacity through process technology improvements. The results of the activity show that the group's integrated waste processing business has developed well according to the installed capacity of Biourine A Plus and In-site Trychocompose (ITC) production. The implementation of activities is broadly in line with the target. The achievement of ITC production has been 56% of installed capacity but seen from the production trend has shown the direction towards actual capacity use. The income of group business has a significant increase that almost double. The constraints faced are mainly caused by fluctuations in the supply of the main raw materials (liquid waste and solid cages) as the cattle population changes. Follow-up efforts are to encourage changes in business orientation into a dual purpose: to maintain the balance of composition between male and female livestock, and expansion of partnerships of raw material input with the aim of non-group cattle farmers.
Pemulihan Ekonomi Rumah Tangga Perkebunan Sawit Rakyat Terkena Dampak Kebakaran Lahan dan Hutan Ardi Novra; Suparjo Suparjo; Abdul Latief; Suhessy Syarif
Jurnal Abdimas Mahakam Vol. 4 No. 1 (2020): Januari
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24903/jam.v4i1.511

Abstract

Kegiatan PPM bertujuan untuk pemulihan ekonomi rumah tangga sawit terkena dampak karhutla tahun 2015. Program PPM mampu memantik partisipasi pihak dunia usaha melalui program CSR yaitu PT. WKS berkontribusi dalam perbaikan jalan produksi dan berkomeitmen menyerap seluruh produk kompos, dan Bank Indonesia berkomitmen memberikan bantuan modal perluasan rumah kompos dan perbaikan kandang guna akselerasi target produksi. S esuai perjanjian kerjasama antara PMJ dan PT. WKS pada saat lounching perdana yaitu 200 ton/bulan dari capaian produksi 50-60 ton/bulan saat ini. Pemasok utama bahan baku limbah usaha pupuk kompos adalah rumah tangga peternak sapi setempat dan desa sekitar. Berdasarkan hasil kegiatan maka dapat disimpulkan bahwa program PPM mampu membangkitkan motivasi pemanfaatan limbah insitu, menjadi sumber pendapatan alternatif guna pemulihan ekonomi pasca karlahut, pemicu partisipasi pihak lain serta merupakan langkah awal menuju kesiapan rumah tangga menghadapi program peremajaan sawit.
KARAKTERISTIK SIFAT KIMIA DAN MIKROBIOLOGI SILASE AMPAS TAHU MENGGUNAKAN TAPIOKA SEBAGAI AKSELERATOR yeni karmila; Yatno Yatno; Suparjo Suparjo; Rasmi Murni
STOCK Peternakan Vol 2, No 1 (2020)
Publisher : Universitas Muara Bungo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36355/sptr.v2i1.367

Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh penggunaan berbagai level tapioka  terhadap kondisi kimia (pH,  Bahan Kering, N-Amonia, Asam Laktat dan Nilai Fleigh) dan mikrobiologi (Populasi bakteri asam laktat) silase ampas tahu. Bahan yang digunakan pada  penelitian ini adalah ampas tahu, tepung tapioka, dan probio-FM. Alat yang digunakan pada penelitian ini adalah kain pemeras, selotip, pH meter, termometer, plastik bening bervolume 2 kg, karet, tali plastik, baskom, dan timbangan.  Metode  penelitian ini dilaksanakan dalam 3 tahap yaitu persiapan alat dan bahan, pembuatan silase dan analisis kualitas silase secara fisik. Rancangan yang digunakan adalah Rancangan Acak Lengkap (RAL) dengan 4 perlakuan dan 5 ulangan. Perlakuan yang  diterapkan  adalah  berbagai  level  penggunaan  tapioka  sebagai  akselerator dalam silase ampas tahu yaitu : T0 (0%), T1 (3%), T2 (6%) dan T3 (9%). Data  yang   dihimpun   dianalisis   menggunakan  analisis  ragam,  apabila  terdapat  hasil  yang  berpengaruh nyata pada perlakuan maka dilanjutkan dengan uji  DMRT  (Duncan Multiple  Range   Test).   Hasil   penelitian  menunjukkan  bahwa  penggunaan  tapioka  sampai  6%  berpengaruh nyata (P<0.05) terhadap Bahan Kering, Nilai pH dan Nilai Fleigh, namun tidak  nyata (P>0.05) terhadap kandungan N-Amonia, asam laktat dan populasi bakteri asam laktat silase ampas tahu.  Hasil penelitian dapat disimpulkan bahwa penggunaan 6% tapioka sebagai akselerator mampu menghasilkan silase ampas tahu berkualitas, yang  ditandainya  dengan  populasi bakteri asam laktat, dan asam laktat yang tinggi, pH asam serta nilai NF yang cukup baik.
Screening and Determination of Potential Cellulolytic Bacteria from Mangrove Ecosystem Ummi Mardhiah Batubara; Suparjo Suparjo; Hasna Ul Maritsa; Eko Pujianto; Meli Herlini
Jurnal Perikanan dan Kelautan Vol 27, No 2 (2022): June 2022
Publisher : Fakultas Perikanan dan Kelautan Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jpk.27.2.264-271

Abstract

The mangrove ecosystem is a coastal wetland resource that has a diversity of biodiversities such as flora, fauna, and microorganisms. Microbes are an important component in the mangrove environment not only play a role in creating and maintaining boosters but also work as a source of biotechnology products. Cellulolytic bacteria are a group of cellulase-producing bacteria capable of breaking down cellulose into glucose monomers, and cellulose as a source of carbon and energy. This study aims to screen and determine the diversity of cellulolytic bacteria from the mangrove ecosystem. The research method was experimentally by exploring the diversity of cellulolytic bacteria from soil and sediment from the mangrove ecosystem. Three sampling locations were selected, such as litter soil, soil in the root area, and soil in the tidal area. Screening of potential bacteria producing cellulase enzymes was carried out by growing bacteria on Carboxy Methyl Cellulose (CMC) agar medium which was cultured for 48 hours at 37°C. The bacteria that produced the greatest cellulolytic activity were characterized by their morphological and physiological characteristics. The results of bacterial characterization were then adjusted according to Bergey's Manual of Determinative Bacteriology. Based on the analysis showed three bacterial isolates were obtained that had the largest cellulolytic activity index, namely MS06 (9.73), MS08 (5.41), and MS02 (5.07). The results showed that three isolates had the same characteristics as the bacterial genera Bacillus, Cellulomonas, and Micrococcus.