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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Kedokteran Brawijaya
Krisni Subandiyah
Laboratorium Ilmu Kesehatan Anak Fakultas Kedokteran Universitas Brawijaya / RSSA Malang
Agriculture, Biological Sciences & Forestry Environmental Science Medicine & Pharmacology

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 1 
Difference of Vitamin D and Interleukin-6 Levels in Children with Steroid- Resistant, Steroid-Sensitive and Idiopathic Nephrotic Syndrome Subandiyah, Krisni; Ghofar, Hervin Febrina; Fitri, Loeki Enggar
Journal of Tropical Life Science Vol 9, No 2 (2019)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (209.072 KB) | DOI: 10.11594/jtls.09.02.07

Abstract

Idiopathic nephrotic syndrome (INS) is the most prevalent autoimmune glomerular disease in children and its pathogenesis is correlated with high level of interleukin 6 (IL-6) and low level of vitamin D. This study was aimed to investigate the difference of vitamin D and IL-6 level in steroid resistant nephrotic syndrome (SRNS), steroid sensitive (SSNS), and idiopathic nephrotic syndrome (INS). This research was designed as cross sectional involving 45 subjects which then divided into 3 groups as follows: SRNS, SSNS, and INS. A level of serum 25 (OH)D was measured by Enzyme-linked Immuno Assay Method then categorized as sufficiency, insufficiency, and deficiency. Level of IL-6 serum was measured by ELISA method. Results showed that IL-6 level was significantly different among three groups, in which SRNS had the highest value (ANOVA, p < 0.05). Further analysis demonstrated that IL-6 level correlated with steroid resistance (Spearman correlation test, p = 0.000, r = 0.692). Vitamin D status was significantly different among three groups (Chi square, p = 0.03) and associated with steroid resistance (Spearman correlation test, p = 0.000, r = 0.568). Moreover, IL-6 level associated with 25 (OH) D level in SRNS group (Pearson correlation test, p = 0.020, r = 0.591) but not in the SSNS and INS group. We conclude that IL-6 levels were significantly higher in SRNS group as compared to other groups. Otherwise, vitamin D status were significantly lower in SRNS compared with other groups. An IL-6 level was negatively correlated with vitamin D status in patients with NS, specifically in SRNS group.
Correlation of Malondialdehyde (MDA) and C-reactive Protein (CRP) Level to Neurodevelopmental Outcome in Children After the Episode of Convulsive Type Status Epilepticus Restuningwiyani, Sintha; Ariani, Ariani; Sujuti, Hidayat; Rahayu, Masruroh; Subandiyah, Krisni
Journal of Tropical Life Science Vol 8, No 3 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (229.772 KB) | DOI: 10.11594/jtls.08.03.07

Abstract

Refractory and mortality associated with status epilepticus (SE) were correlated with the degree of inflammation-induced neuronal cell death. This study was aimed to investigate the correlation of oxidative stress (Malondialdehyde, MDA) and inflammation (C-reactive protein, CRP) process with neurodevelopmental outcome in children after the episode of convulsive type SE. This study was designed as cross sectional which included 26 convulsive type SE subjects and 15 control subjects. MDA level was measured by thiobarbituric acid (TBA) method, while CRP level was measured by ELISA method. Neurodevelopmental outcome was measured by Bayley-III Scale of Infant and Toddler Development 3 month after the convulsive type SE episode. Results showed that both MDA (independent t-test, p &lt; 0.05) and CRP (Mann-Whitney test, p &lt; 0.05) level was significantly higher in convulsive type SE group as compared to control group. Further analysis also showed that MDA (Spearman correlation test, p = 0.000, r = 0.756) and CRP (Spearman correlation test, p = 0.000, r = 0.835) level was positively correlated with convulsive type SE. In convulsive type SE group, MDA level was negatively correlated with neurodevelopmental outcome but CRP was not. We concluded that MDA level was negatively correlated with neurodevelopmental outcome in children with convulsive type SE, but CRP was not.
The Correlation of Regulatory T (TReg) and Vitamin D3 in Pediatric Nephrotic Syndrome Nurtyas, Yunika; Subandiyah, Krisni; Fitri, Loeki Enggar
Journal of Tropical Life Science Vol 8, No 1 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.08.01.08

Abstract

Nephrotic syndrome (NS) is an autoimmune disease that correlates to the imbalance of regulatory T cells (TReg). This study was aimed to investigate the effect of vitamin D as adjuvant therapy of TReg population in pediatric nephrotic syndrome. This study was designed randomized clinical trial, double blind, with pre- and post-test control groups involving 15 subjects newly diagnosed with NS. Subjects were divided into 2 groups, namely K1 for group treated with prednisone+vitamin D and K2 group for prednisone treatment only. The population of TReg in peripheral blood mononuclear cells (PBMC) was analyzed using flowcytometry. Vitamin D serum level was measured through ELISA method. Results showed that there was a significant elevation of TReg (independent t-test, p = 0.010) in K1 group, which was higher than in K2 group. The Pearson test in the K1 group showed that vitamin D level was positively correlated with TReg (p = 0.039, r = 0.779).
Ekspresi dan Aktivasi Reseptor Glukokortikoid serta Ekspresi Heat Shock Protein- 90 pada Sel Limfosit Penderita Sindrom Nefrotik Idiopatik Khairiyadi, Khairiyadi; Subandiyah, Krisni; Fitri, Loeki Enggar
Jurnal Kedokteran Brawijaya Vol 25, No 3 (2009)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (560.895 KB) | DOI: 10.21776/ub.jkb.2009.025.03.5

Abstract

Glucocorticoid receptors (GR) have an important role in the resistance of steroid therapy . Previous studies showed  significant  difference  activation  and  expression  of  GR  between  Steroid  Resistant  Nephrotic Syndrome (SRNS) and Steroid Sensitive Nephrotic Syndrome (SSNS), however the other studies showed contrastive result. Previous studies also revealed Hsp-90 had an important  role in  GR activation. The aim of this study was to determine the differences expression and activation of GR and also Hsp-90 expression in lymphocytes  cytoplasm  and  nucleus  between  SRNS  and  SSNS  patients.  A  Cross-sectional  study  was conducted  to  40  children  (20  SRNS  and  20  SSNS  patients).  Double  stainning  immunocytochemistry  of lymphocytes  was  performed.  The  GR  expression  and  activation  were  determined  by  counting  amount  of lymphocytes  that  express GR and Hsp-90 among 200 under light  microscope with 1000x magnification. The differences  of  activation  and  activation  of  GR  and  Hsp-90  in  cytoplasm  and  nucleus  were  analyzed  with independent t-test and the correlation was analyzed with Pearson correlation.
OUTCOME SINDROM NEFROTIK PADA ANAK – PENELITIAN PROSPEKTIF STUDI COHORT Subandiyah, Krisni
Jurnal Kedokteran Brawijaya Vol 20, No 3 (2004)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (133.193 KB) | DOI: 10.21776/ub.jkb.2004.020.03.6

Abstract

Nephrotic syndrome (NS) in children is a common recurrent disease. Most of the cases are due to minimal change disease with a favorable outcome. The mayority of children have minimal change disease and 90 – 95% will respond to steroid therapy. Response to steroid therapy carries a greater prognostic weightthan the histologic features. The aim of the studywas to describe the outcome of Nephrotic Syndrome in children and to determine risk factors for these complications. Children with NSwere admitted to Pediatric Department Saiful Anwar Hospital Malang, January 2000 - December 2003 evaluated prospectively for one year. Data was sought on steroid responsiveness, remission, relapse rates, infection, and trombosis. Patients were classified into five categories. Including: relapse, infrequent relapsing (IFRNS), frequent relapsing (FRNS), steroid dependent (SDNS) and steroidresistant (SRNS). Baseline age, gender, clinical manifestation and laboratory finding were used to predict category of the disease. Definition of NS, remission and relapse were based on the ISKDC guidelines. Of 91 children with NS, 75 (82,4%) children had steroid sensitive nephrotic syndrome (SSNS) while 16 (17,6%) children were classified SRNS. In the SSNS group 42 (56%) of children were over 6 years of age and there were 51 (68%) males and 24 (32%) females.
Ekspresi dan Aktivasi Sub Unit p65 NF-κB pada Sel Mononuklear Penderita Sindrom Nefrotik Resisten Steroid Anak Corebima, Brigitta Ida RV; Subandiyah, Krisni; Fitri, Loeki Enggar
Jurnal Kedokteran Brawijaya Vol 25, No 3 (2009)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (649.959 KB) | DOI: 10.21776/ub.jkb.2009.025.03.7

Abstract

Resitance  to  steroid  therapy  is  a  poor  prognosis  sign  for  nephrotic  syndrome,  but  this  mechanism is  still unclear. NF-κB has been reported to affect the glucocorticoid at the T cell through the transrepression in  the glucocorticoid  receptor .  Until  know,  this  study  was  still  limited.  The  aim  of  this  study  was  to  show  the  difference expression  and  activation  of  NF-κB  p65  subunit  between  childhood  SRNS  and  SSNS  patient.  Cross  sectional study  was  conducted  during  January  2009  to  December  2009  in  the  Saiful Anwar  General  Hospital  and Biomedic  Laboratory  Brawijaya  University.  Expression  and  activation  of  NF-κB  p65  subunit  were  determine  by immunochemistry  staining  by  counting  the  amount  of  lymphocyte  that  express  and  activate  NF-κB  p65  subunit among  200  lymphocytes  under  light  microscope  with  1000  magnification.  Results  was  analyzed  by independent  t  test  with  95%  CI  (p&lt;0,05)  and  Pearson  correlation.  The  result  of  this  study  revealed  no significant  difference  of  expression  of  NF-κB p65  subunit  between  SRNS and  SSNS  (p=0,153),  but  there  was an increase activation of NF-κB p65 subunit in the SRNS patients (p=0,000).
POLA DAN SENSITIVITAS TERHADAP ANTIBIOTIK BAKTERI PENYEBAB INFEKSi SALURAN KEMIH ANAK DI RSU DR SAIFUL ANWAR, MALANG Subandiyah, Krisni
Jurnal Kedokteran Brawijaya Vol 20, No 2 (2004)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (161.845 KB) | DOI: 10.21776/ub.jkb.2004.020.02.1

Abstract

ABSTRACT Urinary tract infection (UTI) is one of the most common bacterial infections affecting children. Early recognition and prompt treatment of UTIs are important to prevent progression of infections and to avoid late sequeale. The aim of the study was to identify the bacterial agents of urinary tract infections in children and to study sensitivity to antibiotics. Urinary specimens were collected from children suffering from urinary tract infections, who were either inpatients or outpatients between January 1999 and December 2003. Of a total of 563 urine specimens, bacteriuria was found in 276 (49.02%) of patients. Females showed a higher prevalence of infection (51.1%) than  males  (48.9%).  Mean  age  was  63  months,  median age  was  60  months  (range  1  month  to  12  years).  The microorganisms isolated  from  children  included  Escherichia  coli  (48.9%),  Acinetobacter  anitratus  (9.8%),  Klebsiella  pneumoniae  (9.4%), Staphylococcus positive coagulase (5.8%), Proteus mirabilis (4.7%), others (21.4%). Escherichia coli was sensitive to nitrofurantoin (74.8%),  nalidixic  acid  (69.6%),  cefotaksim  (48.9%),  Amoxcillin  clavulanat  acid  (37.8%),  fosfomicin  (35.6%),  gentamicin  (34.1%), ceftriaxone (31.8%), amikacin (19.2%), cotrimoxazole (15.6%), ciprofloxacin (11.1%), cefuroxim (3.7%), netilmicin (8.9%), amoxcilin (2.9%),  chloramphenicol  (2.2%),  ampicilin  (2.2%).  Resistant  to  ampicillin  dan  chloramphenicol  97.8%.  Acinetobacter  anitratus sensitive to nalidixic acid (62.96%), Amoxcillin clavulanat acid (48.15%), gentamicin (40.74%), co-trimoxazole (33.33%), nitrofurantoin (25.93%), amoxcilin (25.93%), amikacin (11.11%), netilmycine (11.11%), ampicillin (7.40%), ciprofloxacin (7.40%), cefotaxim (7.4%), ceftriaxon (7.4%), cefuroxim (3.7%).
The Correlation Between IL-1β and IL-10 Levels in Estimating The Risk of Febrile Seizures Gunawan, Andi; Muid, M; Suyudi, Hidayat; wisnu, Barlianto; Subandiyah, Krisni
Journal of Tropical Life Science Vol 4, No 2 (2014)
Publisher : Journal of Tropical Life Science

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Abstract

Febrile seizures are the most common forms of seizures in children with fever. A data from clinical and experimental studies suggest the potential role of immune generate products in their genesis. The balance between proinflammatory (IL-1β) and anti-inflammatory (IL-10) cytokines influences the regulation of infections and plays a role in the pathogenesis of febrile seizures. In existing literature, there is no research on the correlation between IL-1β and IL-10 levels as well as the ratio of IL-1β to IL-10 in estimating the risk of febrile seizures in seizure prone children aged between 3 months and 5 years. In this study, IL-1β, IL-10 levels and ratio of IL-1β to IL-10 in the risk of febrile seizures were investigated, and respondents were divided into three groups: febrile seizures, febrile without seizures and healthy children without histories of febrile seizure. This study has used A cross-sectional design, and each group had 17 co-respondents. IL-1β and IL-10 levels were measured by ELISA. Data were analyzed by SPSS 15. Findings showed there were significant differences between IL-1β and IL-10 as well as in the ratio of IL-1β to IL-10 between febrile seizure patients and healthy children. It was concluded that there were significant correlations between IL-1β and IL-10 levels as well as IL-1β to IL-10 ratios in estimating the risk of febrile seizures.
Toll Like Receptor 4 (Tlr4) And p65 Nuclear Factor Kappa B (Nf-Kb) Expression In Monocyte Cell Of Children With Steroid Resistant Nephrotic Syndrome Wahyono, Tikto; Subandiyah, Krisni; Fitri, Loeki Enggar; Endharti, Agustina Tri
Journal of Tropical Life Science Vol 4, No 2 (2014)
Publisher : Journal of Tropical Life Science

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Abstract

Vitamin D3 Adjuvant Treatment Stimulate Interleukin-10 Expression in Children with Nephrotic Syndrome Without Affecting to Clinical Outcome and Glucocorticoid Receptor Expression Asariati, Husnul; Subandiyah, Krisni; Fitri, Loeki Enggar
Journal of Tropical Life Science Vol 4, No 3 (2014)
Publisher : Journal of Tropical Life Science

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Abstract

Co-Authors Agustina Tri Endharti Andi Gunawan Ariani Ariani Brigitta Ida RV Corebima Ghofar, Hervin Febrina Hidayat Sujuti Husnul Asariati Khairiyadi Khairiyadi Loeki Enggar Fitri Masruroh Rahayu, Masruroh Muid, M Nurtyas, Yunika Restuningwiyani, Sintha Suyudi, Hidayat Wahyono, Tikto wisnu, Barlianto