Jarot Subandono
Biochemistry Laboratory, Medical Faculty of Sebelas Maret University, Surakarta.

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Journal : Nexus Biomedika

Efek Perlindungan Susu Kedelai (Glycine max) Ultra High Temperature (UHT) Terhadap Lambung Mencit Wijnaputri, Ginong Pratidina; Listyaningsih, Endang; Subandono, Jarot
Nexus Biomedika Vol 1, No 2 (2012): Nexus Biomedika
Publisher : Nexus Biomedika

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Background: White Soybean (Glycine max) Milk Ultra High Temperature (UHT) is potential to protect gaster because it contains flavonoid. This study aimed to investigate the protective effect of white soybean milk UHT on mice’s gaster due to aspirin. Higher dose of the milk reduces the damaged on gastric mucous induced by aspirin Methods: This was a labroratory experimental research with posttest only controlled group design conducted in in Histology Laboratory of Sebelas Maret University. The samples were thirty five mice divided into 5 groups. Negative control group (KN) was given normal food and aquadest, positive control group (KP) was given aspirin dose 2,275 mg/20g Body weight (W), dan first treatment group (P1) was given cimetidine dose 0,78 mg/20g W second treatment group (P2) and third treatment group (P3) was given soybean milk UHT dose 0,7ml/20 g W and 1,4 ml/20 g W. All treatments for KN, KP, P1 , P2, P3 was given in 10 days. Aspirin was given to KP, P1 , P2, P3 with dose 2,275 mg/20g weight of mice on day 8, 9 and 10. to evaluate the  level of damage based on histologic appearance, gastric staining was performed using Haem eos. Results: Kruskal-Wallis test result showed significant result among four groups. Mann-Whitney showed significant result for KN compared to KP,P1,P2 , P3 and KP compared to P1, P2 and P3 ; while P1 compared to P2, P3 and P2 compared to P3 had no significant result. Conclusion: White Soybean (Glycine max) Milk Ultra High Temperature(UHT) protect mice’s gastric from gastritis. However, higher dose of White Soybean (Glycine max) Milk Ultra High Temperature(UHT) does not increase the protective effect of white soybean milk UHT on mice’s gaster due to aspirin. Keywords: White Soybean (Glycine max) Milk Ultra High Temperature(UHT), gastric protection, gastritis, aspirin. 
Pengaruh Pemberian Ekstrak Daun Sambiloto Terhadap Kadar Kolesterol LDL Darah Tikus Putih (Rattus norvegicus) Anggraeni, Yohana Trissya; Subandono, Jarot; Kustiwinarni, .
Nexus Biomedika Vol 2, No 3 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

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Abstract

Background: Andrographis paniculata,Nes leaves contain flavonoids, beta-sitosterol, and andrographolide which can lower LDL cholesterol level of blood. The objective of this research was to determine the effect of Andrographis paniculata,Ness leaves extract against blood LDL cholesterol level of white rats (Rattus norvegicus). Methods: This study was a laboratory experimental research design, using posttest only controlled group design. Subjects of this research were white rats (Rattus norvegicus), strain Wistar, male, 3 months old, 200 grams weight. By incidental sampling technique, data were collected from 25 rats. Those rats were arranged into 5 groups, each group consisted of 5 rats. The normal control group (KKn) was administered distilled water, while the negative control group (KKn(-)) and the 1st (KP1), 2nd (KP2) and 3rd treatment group (KP3) were administered high cholesterol diet (6.25 g/kg BW/day of egg yolk). Besides that, the KP1, KP2 and KP3 were treated with 160 mg, 320 mg, and 640 mg per 200 g BW of Andrographis paniculata,Ness leaves extract, successively. This research was held for 28 days. Data of blood LDL cholesterol levels were collected 10-12 hours after the last treatment. Later, the data was analyzed by One-Way ANOVA test (α = 0.05) and Posthoc Multiple Comparisons (LSD) test (α = 0.05). Results: The one-way ANOVA test showed a significant difference among five groups p = 0.000 (p <0.05). Whereas, the LSD test showed significant differences between KKn-KKn(-), KKn-KP1, KKn-KP2, KKn-KP3, KKn(-)-KP1, KKn(-)-KP2, KKn(-)-KP3, (p < 0.05) and non-significant differences between KP1-KP2, KP1-KP3, and KP2-KP3 (p > 0.05). Conclusions Andrographis paniculata,Ness leaves extract significantly lowered blood LDL cholesterol levels of Rattus norvegicus but the increase of the dose did not enhance  its function in lowering blood LDL cholesterol. Keywords: Andrographis paniculata Ness leaves extract, LDL cholesterol