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An Isoflovonoid, Warangalone from the Stem Bark of Dadap Ayam (Erythrina variegata) Herlina, Tati; Nasrudin, Nasrudin; Supratman, Unang; Subarnas, Anas; Sutardjo, Supriyatna; Hayashi, Hideo
Jurnal ILMU DASAR Vol 9 No 1 (2008)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

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Abstract

In the course of our continuing search for novel paralytic compound from Indonesian plants, the methanol extract of the stem bark of Erythrina variegata (Leguminosae) showed significant paralytic activity against the third instar larvae of silkworm (Bombyx mori). The purposes of this research were isolation and structural elucidation of paralytic compound from the stem bark of E. variegata. Using the paralytic activity following the separation, the methanol extract was separated by combination of column chromatography to yield prenylisoflavone, warangalone. The chemical structure of warangalone was identified based on spectroscopic evidence and comparison with the previous reported. The paralytic activity of warangalone showed weak activity against the third instar larvae of silkworm (B. mori).
In Vitro Anti-Cancer Alkaloid and Flavonoid Extracted from the Erythrina variegata (Leguminoseae) Plant Herlina, Tati; Supratman, Unang; Subarnas, Anas; Sutardjo, Supriyatna; Amien, Suseno; Hayashi, Hideo
Indonesian Journal of Cancer Chemoprevention Vol 2, No 3 (2011)
Publisher : Indonesian Research Gateway

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Abstract

Erythrina plants, locally known as “dadap ayam”, are higher plant species and have been used as a folk medicine for treatment of cancer. To prove the effectiveness of the leaves and stem bark of E. variegata as an anti-cancer agent, the assay in this research was focused on in vitro  test  towards  breast  cancer  cell  T47D.  In  the course  of  our  continuing  search  for novel anti-cancer agent from Erythrina plants, the methanol extract of the leaves and stem bark of  E. variegata  showed  significant  anti-cancer  activity  against  breast  cancer  cell  T47D  in  vitro  using the Sulphorhodamine B (SRB) assay. By using the anti-cancer activity to follow the separations, the methanol extract was separated by combination of column chromatography. The chemical structure  of  an  anti-cancer  compounds  were  determined  on  the  basis  of  spectroscopic evidence  and  comparison  with  the  previously  reported  and  identified  as  an  erythrina  alkaloid (1)  and  isoflavonoid  (2).  Compounds  (1-2)  showed  anti-cancer  activity  against  breast  cancer cell  T47D  used  with  IC50  of    1.0  and  3.3  µg/mL,  respectively.  This  results  strongly  suggested that E. variegata is promising sources for anti-cancer agents.Keywords: Anti-cancer, Erythrina variegata, Leguminoseae
Aktivitas Antimalaria Daun Erythrina variegata Herlina, Tati; Supratman, Unang; Subarnas, Anas; Sutardjo, Supriyatna; Abdullah, Noor Rain
Jurnal Natur Indonesia Vol 10, No 1 (2007)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jnat.10.1.36-41

Abstract

The leaves of Erythrina variegata (Leguminosae) used tradisional plant of an antimalarial. In the course of our continuing search for novel an antimalarial compound from Erythrina plants, the methanol extract of the leaves ofE. variegata showed significant antimalarial activity in vitro toward Plasmodium falciparum in vitro using the lactate dehydrogenase (LDH) method. The methanol extract of the leaves of E. variegata showed against bothstrains of parasite with IC50of 6.8 ?g/ml against K1 and > 60 ?g/ml against 3D7, respectively. The methanol extract of the leaves of E. variegata was separated by using bioassay-guide fractionation. The n-buthanol fraction yieldedthe most activity, exhibiting equipotency against both strains of parasite with IC50of 5.1 ?g/ml against K1 and 13.5 ?g/ml against 3D7, respectively. Furthermore, by using the antimalarial activity to follow separation, the n-buthanol fraction was separated by combination of column chromatography to yield an active compound. The active compound showed antimalarial activity against both strains of parasite used with IC50 of 4.3 ?g/ml against K1 and 23.5 ?g/ml against 3D7, respectively. Its inhibition of the resistant strain (K1) was also much better compared to its inhibition of the sensitive strain (3D7), indicated that the leaves of E. variegata to be potential as antimalarial agents, but its lower potency compared to artemisinin and chloroquin.
Validation of UV-Vis spectrophotometry methode for analysis of apigenin in celery extract (Apium graveolens L.) Yulianto, Asep Nurrahman; Rusdiana, Taofik; Muchtaridi, Muchtaridi; Subarnas, Anas
Pharmaciana Vol 7, No 2 (2017): Pharmaciana
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (319.673 KB) | DOI: 10.12928/pharmaciana.v7i2.7124

Abstract

Apigenin is one of the flavonoid compounds contained in celery (Apium graveolens L.) and has been used for the treatment of various diseases. This study aims were to ensure the validity and accuracy of analytical method for apigenin assay in celery extract using UV-Vis spectrophotometry. The flavonoids test of celery extract give a positive result which produces a yellow color that can be drawn by amyl alcohol. The result of TLC test using a mobile phase of chloroform and methanol (9.5 : 0.5) produced Rf value of 0.75. Validation of analytical methods were sensitivity, linearity, limit of detection as well as limit of quantification, accuracy, and precision. Validation of analytical methods showed that sensitivity results as represented by molar extinction (ε) was 77004.35 M-1cm-1. Linearity from calibration curve at concentration of 2, 4, 6, 8, and 10 mgL-1 resulted an equation of y = 0.074x – 0.0011 with coefficient correlation (r) of 0.9996. The limit of detection and limit of quantification were 2.99 mgL-1 and 9.92 mgL-1 respectively with recovery percentage of 97.59% and the coefficient variation values were 1.53%; 2.43% and 1.68%. The average contents of apigenin in the celery extract were of 3.74 ± 0.09%. 
PENANDAAN DAN BIODISTRIBUSI RADIOFARMAKA TERAPI KANKER OTAK 1251-NIMOTUZUMAB TERHADAP MENCIT NORMAL Ismuha, Ratu Ralna; Ramli, Martalena; Gunawan, Adang Hardi; Mutalib, Abdul; Subarnas, Anas
Jurnal Radioisotop dan Radiofarmaka Vol 13, No 2 (2010): Jurnal PRR 2010
Publisher : Jurnal Radioisotop dan Radiofarmaka

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Abstract

ABSTRAKPENANDAAN DAN BIODISTRIBUSI RADIOFARMAKA TERAPI KANKER OTAK 125I-NIMOTUZUMAB TERHADAP MENCIT NORMAL. NIMOTUZUMAB adalah anti bodi monoclonal yang diketahui berperan banyak dalam antiproliferasi, proapoptisis dan efek antiangionik pada terapi kanker  otak  (glioma). Penandaan antibody monoklonal nimotuzumab dengan radiosiotop 1251yang memancarkan e1ektron auger telah dilakukan dengan menggunakan metode  iodogen. Hasil penelitiaan kemurnian radiokimia terbaik (97%)  menunjukan  mol  ratio protein (nimotuzumab) dengan kalium iodida  dan  lodogen adalah 1: 2: 1200  pada fraksi ke 6.  Kemurnian radiokimia diuji dengan  kromatografi kertas dengan fase diam  dan  fase gerak  masing –masing  adalah  kertas whatman  no. 1 dan  etanol-butanol-amonium  hidroksida dengan  perbandingan  3 : 2 : 1 Nilai Rf untuk  1251_nimotuzumab adalah 0,0 dan  125I adalah 0,9.  Hasil uji biodistribusi terhadap mencit normal selama 72 jam  menunjukan 125 I-nimotuzumab memiliki  waktu  paruh  yang  panjang  dan  terakumulasi  tinggi dalam organ  hati (1,97 ± 1,18%), organ ginjal (0,82± 0,28%)  dan  otot (0,61±,98%).  Sedangkan  akumulasi tertinggi  pada organ otak mencit normal (0,13 ±0,06%) baru terjadi setelah 24jam penyuntikan. Berdasarkan efek terapi dan akumulasi terhadap organ pada mencit normal, 1251_9imotuzumab berpotensi digunakan untuk terapi kanker otak. Kata kunci: Biodistribusi, Glioma, 1251,lodogen, Nimotuzumab ABSTRACT LABELING AND BIODISTRIBUSTION OF THERAPEUTIC RADIOPHARMACEUTICAL FOR BRAIN CANCER 125I – NIMOTUZUMAB IN NORMAL MICE. Nimotuzumab is a monoclonal antibody which known giving contribution in antiproliferation, proapoptosis and antiangionik effect on the therapy of brain cancer (glioma). The labeling of monoclonal antibody nimotozumab with 1251which radiate Juger electrons has been done with idogen method. The best result of radiochemical purity (97%) was shown ihI fraction 6 with the mol ratio of nimotuzumab towards potassium iodide and iodogen was 1 : 2 : 1200. Radiochemical purity was examined by paper chromatography with whatman paper no. 1 as the stationary dhase and ethanol-butanol-ammonium hydroxide with a ratio of 3 : 2 : 1 as the mobile phase. Rf Values for 1251-nimotuzumab is 0.0, while Rf value of 1251is 0.9. The biodistribution result on normal mice for 72 hours showed that 1251-nimotuzumab not only has a long half-life time but also has high accumulation in liver (1.97 e. 1.18(%0.)kidey (0.82±0.28%) and  muscle ( 0.61 ± 0.98 % ). The higest accumulation in liver (1.97 mice ( 0.128±0.06% ) occurred 24 hours after injection . Based on trhwe therapeutic effect and organ accumulation on normal mice,  1251-nimotuzumab could be potentially used for brain cancer therapy. Keywords: Biodistribution, Glioma, 1251,lodogen, Nimotuzumab      
The determination of quercetin in Plectranthus scutellarioides(L.) R.Br. leaves extract and its In SilicoStudy on Histamine H4 Receptor Moektiwardoyo, Moelyono; Levita, Jutti; Sidiq, Syafrudin Purnama; Ahmad, Khoziah; Mustarichie, Resmi; Subarnas, Anas; ., Supriyatna
INDONESIAN JOURNAL OF PHARMACY Vol 22 No 3, 2011
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (337.578 KB) | DOI: 10.14499/indonesianjpharm0iss0pp191-196

Abstract

Plectranthus  scutellarioides  (L.)  R.Br.,  or  jawer  kotok,  Family  Lamiaceae, grows  widely  in  Indonesia,  and  has  a  long  history  of  therapeutic  usage  in Indonesian traditional jamuto cure various diseases. The brownish purple leaves of  Plecranthus  contain  alkaloids,  saponin,  flavonoids,  tannin,  volatile  oils,  and quercetin  which  has  been  proven  to  exert  antiinflammatory  activity.  In  this research,  a  determination  of  quercetin  in  Plecranthus  leaves  extract  was performed and followed by a study of its interaction with histamine H4 receptor to  understand its  anti-inflammatory  activity.  The  dry  leaves  were  macerated by using  a  mixture  of  methanol  and  water  (1:1)  for  48  hours  and  the  solvent  was evaporated  at  low  temperature  (40-50oC).  Analysis  of  quercetin  in  the  extract was performed by using reversed-phase HPLC method LC-10AT VP (Shimadzu), Atlantis  Hilicsilica  C18  (Waters®)  150  mm  x  4.6  mm,  5  µm  as  stationary  phase and  a  mixture  of  acetonitrile,  phosphoric  acid,  and methanol  (40:50:10),  flow rate 0.8 mL/minute.  In silicostudy of quercetin with histamine H4 receptor was performed by using AutoDock Tools 3.0.5. Histamine H4 receptor (H4R) belongs to  G  protein-coupled  receptors  which  is  involved  in arthritis,  asthma,  and inflammations.  The  3D  structure  model  of  H4R  was  built  by  using  MODELLER 9v7.  Quercetin  contained  in  Plecranthus  leaves  extract  was  0.05  %.  This compound interacted with H4R viahydrogen bond formation with Lys158 (2.006 Å)  and  Glu182  (2.048  Å),  and  van  der  Waals  interaction  with  Trp90,  Leu91, Asp94, Tyr95, Phe168, Thr178, Ser179, Tyr319, Phe344, and Tyr340, therefore Plecranthus  leaves  extract  might  have  a  chance  to  be  used  as  histamine  H4 receptor inhibitor.Key  words  :   histamine  H4  receptor,  in  silico  study,  Plecranthus  leaves,  Plectranthus scutellarioides, quercetin
Peran Kepuasan Mutu Layanan Farmasi dalam Peningkatan Loyalitas Pasien di Rumah Sakit Al Islam dan Santo Yusup Kota Bandung Kurniasih, Dea A. A.; Subarnas, Anas; Djuhaeni, Henni
Indonesian Journal of Clinical Pharmacy Vol 4, No 3 (2015)
Publisher : Indonesian Journal of Clinical Pharmacy

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (203.332 KB) | DOI: 10.15416/ijcp.2015.4.3.206

Abstract

Instalasi farmasi di rumah sakit merupakan salah satu pelayanan utama karena lebih dari 90% pelayanan kesehatan di rumah sakit menggunakan perbekalan farmasi sehingga pelayanan farmasi yang kurang bermutu akan menimbulkan kerugian. Mutu pelayanan farmasi akan berperan terhadap kepuasan dan selanjutnya terhadap loyalitas. Tujuan penelitian ini untuk menganalisis peran kepuasan atas mutu layanan farmasi dalam peningkatan loyalitas pasien rawat jalan di Rumah Sakit Al Islam dan Santo Yusup Kota Bandung. Rancangan penelitian yang digunakan adalah analitik observasional dengan pendekatan potong lintang yang dilakukan pada bulan September 2013 hingga Mei 2014. Instrumen yang digunakan adalah kuesioner dengan skala Likert. Pengambilan sampel dilakukan secara purposive sampling menggunakan 100 responden InHealth Rumah Sakit Al Islam, 103 responden InHealth Rumah Sakit Santo Yusup, dan 100 responden umum pada Rumah Sakit Al Islam Kota Bandung sebagai kontrol. Teknik analisis yang digunakan adalah model persamaan struktural dan analisis varians multivariat. Hasil penelitian menunjukkan bahwa pada pasien InHealth Rumah Sakit Al Islam, kepuasan atas bukti langsung, empati, daya tanggap, dan jaminan yang berperan dalam peningkatan loyalitas, pada pasien InHealth Rumah Sakit Santo Yusup hanya kepuasan atas bukti langsung dan daya tanggap yang berperan dalam peningkatan loyalitas sedangkan pada pasien umum Rumah Sakit Al Islam kepuasan atas bukti langsung, keandalan, dan jaminan yang berperan dalam peningkatan loyalitas.Kata kunci: Kepuasan, loyalitas, mutu layanan farmasi, rumah sakitThe Role of Satisfaction for Pharmaceutical Service Assurance to Improve Patient Loyalty at Al Islam and Santo Yusup Hospital in BandungPharmacy is one of the main services in hospital because more than 90% health services using pharmacy supplies, therefore pharmaceutical service which is not qualified would bring disadvantage to hospital. The quality of pharmacy service would impact to satisfaction, and furthermore will affect loyalty. The aim of this study was to analyze the role of satisfaction from pharmaceutical service quality to improve loyalty out-patient care in Al Islam and Santo Yusup hospital in Bandung. This study used observationalanalytical with cross sectional approachment in September 2013 until May 2014. Questionnaire with Likert’s scale was used as the instrument of this study. Purposive sampling was used with 100 samples from InHealth Al Islam Hospital patient, 103 samples from InHealth Santo Yusup Hospital patient and 100 samples from general out-patient care from Al Islam Hospital in Bandung, analyzed with Structural Equation Model and Multivariate Analysis of Variance. The results showed that in Al Islam Hospital InHealth out-patient care satisfaction of tangibles, empathy, responsiveness, and assurance improving loyalty, in Santo Yusup Hospital InHealth out-patient care satisfaction of tangibles and responsiveness improving loyalty. On the other hand, in general out-patient care Al Islam Hospital satisfaction of tangibles, reliability, and assurance improving loyalty.Keywords: Hospital, loyalty, pharmaceutical service, satisfaction
Antioxidant Activity of Combination Ethanol Extract of Turmeric Rhizome (Curcuma Domestica Val) and Ethanol Extract of Trengguli Bark (Cassia Fistula L) with DPPH Method Triyono, Triyono; Yohana Chaerunisaa, Anis; Subarnas, Anas
Indonesian Journal of Pharmaceutical Science and Technology Vol 5, No 2 (2018)
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (395.508 KB) | DOI: 10.24198/ijpst.v5i3.14764

Abstract

Turmeric rhizome (Curcuma domestica Val) and Trengguli bark (Cassia fistula L) contain antioxidant compounds which can be determined by 1,1-Diphenyl-2- Pycrylhydrazyl (DPPH) free radical inhibition method. This research was conducted to determine DPPH free radical inhibition by ethanol extract of turmeric rhizome, the ethanol extract of trengguli bark, and a combination of turmeric rhizome extract - trengguli bark extract (1:1,5) with ascorbic acid as a comparison. Identification of secondary metabolite classes is performed by phytochemical screening. Antioxidant activity was performed by inhibition of free radical color of DPPH using UV-Vis spectrophotometry. The study showed IC50 value of ascorbic acid, as a comparasion, is 3,14 μg/mL. While ethanol extract of trengguli bark has the best antioxidant activity with IC50 value 10,98 μg/mL compare to combination ethanol extract of turmeric rhizome - trengguli bark (1 :1,5) and ethanol extract of turmeric rhizome with IC50 value is 13,70 μg/mL and 41,95 μg/mL, respectively.Keywords: Antioxidant, Curcuma domestica, Cassia fistula, DPPH Method
AKTIVITAS ANTIOKSIDAN EKSTRAK dan FRAKSI KULIT BATANG TRENGGULI (Cassia fistula L) DENGAN METODE DPPH Najihudin, Aji; Chaerunisaa, Anis; Subarnas, Anas
Indonesian Journal of Pharmaceutical Science and Technology Vol 4, No 2 (2017)
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (208.972 KB) | DOI: 10.15416/ijpst.v4i2.12354

Abstract

Kulit batang trengguli (Cassia fistula L) mengandung senyawa yang bersifat antioksidan yang dapat ditentukan dengan metode peredaman radikal bebas 1,1-Difenil-2- Pikrilhidrazil (DPPH). Penelitian ini dilakukan untuk mengetahui adanya peredaman radikal bebas DPPH oleh ekstrak etanol kulit batang trengguli (EEKBT), fraksi Etil asetat (FEAKBT), fraksi n-Heksan (FnHKBT), fraksi air (FAKBT) dan vitamin C sebagai pembanding. Identifikasi golongan senyawa dilakukan dengan penapisan fitokimia. Fraksinasi dilakukan dengan metode ekstraksi cair-cair dengan tingkat kepolaran yang berbeda. Aktivitas antioksidan dilakukan dengan peredaman warna radikal bebas DPPH menggunakan spektrofotometri UVVis. Penelitian menunjukkan bahwa Fraksi etil asetat memiliki aktivitas antioksidan paling baik dengan nilai IC50 3,980 μg/ml dibandingkan vitamin C 4,716 μg/ml. Sedangkan nilai IC50 ekstrak etanaol, fraksi n-heksan, fraksi air adalah 10,613 μg/ml, 38,904 μg/ml dan 7,636 μg/ml terhadap peredaman warna DPPH.Kata kunci: Antioksidan, Cassia fistula, Fraksinasi, metode DPPH
Korelasi Faktor Usia, Cara Minum, dan Dosis Obat Metformin terhadap Risiko Efek Samping pada Penderita Diabetes Melitus Tipe 2 Riwu, Magdarita; Subarnas, Anas; Lestari, Keri
Indonesian Journal of Clinical Pharmacy Vol 4, No 3 (2015)
Publisher : Indonesian Journal of Clinical Pharmacy

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (324.662 KB) | DOI: 10.15416/ijcp.2015.4.3.151

Abstract

Metformin merupakan obat antidiabetes oral yang umumnya direkomendasikan sebagai pengobatan lini pertama pada diabetes melitus tipe 2 apabila kadar glukosa darah tidak terkontrol dengan modifikasi gaya hidup. Pada penggunaan metformin sebagai kontrol glikemia sering terjadi reaksi obat yang merugikan (ROM) berupa gangguan gastrointestinal seperti diare, mual, dan perut kembung. Penelitian ini bertujuan untuk menganalisis korelasi faktor usia, cara minum, dan dosis metformin terhadap risiko efek samping gangguan gastrointestinal pada penderita rawat jalan BPJS Kesehatan yang baru terdiagnosis diabetes melitus tipe 2 di RSAU Dr. M. Salamun Bandung. Penelitian ini adalah analitik observasional dengan desain potong lintang. Data dikumpulkan dari bagian poliklinik penyakit dalam, rekam medis, dan form check penderita yang mendapat pengobatan dengan metformin yang dilakukan sejak April–Juni 2014. Jumlah penderita yang memenuhi kriteria penelitian sebanyak 65 orang dengan rentang usia rata-rata 48 tahun. Keluhan efek samping yang dialami penderita berupa kembung (58,46%) dan mual (41,54%). Cara minum dan dosis metformin berkorelasi terhadap risiko efek samping berupa mual dan kembung pada penderita diabetes melitus tipe 2 (p<0,05) sedangkan faktor usia tidak berkorelasi (p>0,05). Penggunaan metformin dianjurkan sesudah makan dan dengan dosis awal rendah yang dititrasi perlahan untuk mengurangi dan menghindari terjadinya efek samping mual dan perut kembung pada penderita diabetes melitus tipe 2.Kata kunci: Diabetes melitus tipe 2, efek samping, metforminThe Correlation of Age Factor, Administration, and Metformin Dose Against Risk of Side Effect on Type 2 Diabetes MellitusMetformin is an antidiabetic oral medicine commonly recommended as first line treatment on type 2 diabetes mellitus. Metformin can caused drug related problems (DRPs) such as gastrointestinal disorders, e.g. diarrhea, nausea, and flatulence. This study aimed to analyze correlation profiles on age, administration, and metformin dosage factors against risk of gastrointestinal disorders among newlydiagnosed diabetic outpatients of National Health Insurance in RSAU Dr. M. Salamun Bandung. This study was an analytic observational study with a cross sectional method. The study was carried out in the internal medicine outpatient clinic and data were extracted from patients medical records from April to June 2014. Metformin-treated patients were interviewed using a form check. The number of patients were 65 with the median rate was 48 years old. Side effect reported were flatulence (58.46%) and nausea (41.54%). Administration and metformin dosage factors were correlated to the risk of side effects such as nausea and flatulence on type 2 diabetes mellitus (p<0.05), while age was not correlated (p>0.05). The administration of metformin is recommended after meals and with a lower initial dose titrated slowly to reduce and avoid the side effects of nausea and flatulence in patients with type 2 diabetes mellitus.Keywords: Metformin, side effect, type 2 diabetes mellitus