Didik T. Subekti
Balai Besar Penelitian Veteriner, JL. RE Martadinata 30, Bogor 16114, Indonesia

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Journal : Jurnal Ilmu Ternak dan Veteriner

Parasitaemia pattern and mortality of mice infected by Indonesian Isolate of Trypanosoma evansi. DH, Sawitri; Subekti, Didik T.; AH, Wardhana; ., Suhardono
Indonesian Journal of Animal and Veterinary Sciences Vol 18, No 4 (2013)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2410.495 KB) | DOI: 10.14334/jitv.v18i4.334


Trypanosomiasis (Surra) is one of the parasitic diseases is endemic and deadly for horses and buffalo in Indonesia.The etiology of the disease is a Trypanosoma evansi. Some T. evansi isolates had been isolated and cryopreservated. Those isolates had not been studied for their differences in virulence, particularly with regard to the pattern of parasiteaemia and their ability to promote mice mortality. Therefore in this study the differences in virulence was studied. DDY mice were divided in to 19 groups according to each isolate to be tested. Each group consisted of 5 mice. Infection were carried intraperitoneally at a dose of 104 Trypanosoma/mice. Mice were examined every two days. Blood samples were taken from tail’s peripheral blood and were examined under light microscope. Parasite were quantitatively counted using Naubauer chamber.  Parasitemia and mice survival were observed for 30 days or until all mice died.The results indicated that there was significant difference among the isolates.Through out the nineteenth isolate scan could be grouped into 3 different biotypes associated with patterns of parasitemia and their ability to kill mice. Biotype1 was the most virulent with the ability to promote mice mortality ≤ 8 days post-infection (dpi). The biotype2 and 3 were the lowest compared to biotype 1. Biotype2 had an undulating parasitaemia, where as biotype 3 showed persistently high parasitaemia with the ability to promote mice mortality ≥ 14dpi. The results also indicate the presence of mixed infections of biotypes that exist in one isolate of T. evansi. Key Words: Trypanosoma evansi, Biotype, Virulence, Mixed infection, Parasitaemia
Comparison of serological test for toxoplasmosis using Immunostick Assay, ELISA and Latex Agglutination Test Subekti, Didik T.; Kusumaningtyas, E.
Indonesian Journal of Animal and Veterinary Sciences Vol 16, No 3 (2011)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (233.755 KB) | DOI: 10.14334/jitv.v16i3.617


Many serological methods were developed in order to improve detection and identification of an infectious diseases. ELISA (enzyme linked immunosorbent assay), is a well-known as the most serological technique and widely used for infectious disease diagnoses, especialy toxoplasmosis in human and animal. Unfortunately, the technique requires specific tools and expertise that are not always available in the field. Inn addition, ELISA needs longer process than other techniques for both small and large number of samples. Another serological assay, namely Latex Agglutination Test (LAT) was known as populer and mostly applied in field for toxoplasmosis diagnosis in Indonesia, both for human and animal serum. However, the accuracy of this technique was slightly lower compared to ELISA, especially for weakly seropositive. Recently, an immunostick assay technique was developed as alternative serological method which has high accuracy as ELISA. The immunostick assay was rapid and simple like latex agglutination test. The immunostick assay was evaluated to detect Toxoplasma gondii infection in goat serum samples and compared to ELISA and LAT. The immunostick assay had a true agreement 95.88% - 100% againts ELISA and their strength of agreement was very good aggreement with ELISA (k = 0.796; AC1 = 0.948). However, LAT had true agreement only 75.74%, either with ELISA or immunostick assay. The strength of agreement of LAT was moderately aggreement with ELISA or immunostick assay (k = 0.447; AC1 = 0.582). Therefore, the immunostick assay could become the first choice for rapid serological assay on toxoplasmosis diagnosis, both in the laboratory and in the field. Key Words: Latex Agglutination, ELISA, Immunostick Assay, FELISA, Toxoplasmosis