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Antigenisitas, Sensitivitas, dan Spesifisitas Protein Toxocara canis pada Pemeriksaan Antibodi Serum Mencit dengan Indirect-ELISA Sri Subekti Bendryman; Kusnoto Kusnoto; Tutik Juniastuti
Jurnal Veteriner Vol 16 No 1 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of this research were to determine antigenicity, sensitivity, and specificity of Toxocara canisprotein used as antigen in indirect-ELISA for the detection antibody against the worm in the infected hostin order to proper diagnose kit. The design used was true experimental, with Post-test Only ControlGroups Design. Mouse was immunized with various worm homogenates used to antigenicity, sensitivityand specificity tests of T. canis protein with indirect-ELISA technique. The independence variable werevarious immunogens (homogenates); the dependence variables were antigenicity, sensitivity and specificityvalues interpreted by optical density (OD) value of mouse sera; and controlled variable were mouse strain,feed and retrieval time of sera. The result showed that OD values of mouse sera immunized with T. canisand T.cati homogenate were signicantly difference (p<0.01) as compared to those immunized withAncylostoma spp., Dipylidium caninum and control sera. Using the diagnosis based on the finding ofToxocara, the sensitivity of OD value by ELISA result from mouse sera immunized with Toxocara spp.homogenate were 100%. Using negative OD value by ELISA from mouse sera immunized with Ancylostomaspp. and D. caninum homogenate, the specificity of the test was 87.5%. In conclusion, protein of T.canishas the same antigenicity against anti-T. canis and anti-T. cati sera, but they had the lower antigenicityagainst anti-Ancylostoma spp. and anti-D.caninum sera. As the sensitivity value of 100% and specificityvalue of 87.5%, in detecting antibody against toxocariasis, the possibility of obtaining false positive was12.5%.
UNTILIZATION OF Moringa oleifera LEAF EXTRACT ON DECREASING INFESTATION OF Argulus japonicus IN GOLDFISH (Carassius auratus) Setyawati, Febri; Kismiyati, Kismiyati; Subekti, Sri
AQUASAINS Vol 8, No 1 (2019)
Publisher : Jurusan Perikanan dan Kelautan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (124.157 KB) | DOI: 10.23960/aqs.v8i1.p769-774

Abstract

Abstrak Goldfish (Carassius auratus) is one of the fishes that can be infected by parasites. Commonly, this fish is often attacked by Argulus japonicus ectoparasites. Control of infestation in A. japonicus can be done by utilizing one of the natural ingredients, Moringa oleifera leaf extract, which contains of alkaloids, tannins, saponins and flavonoids. This study aims to determine the effect of M. oleifera leaf extract in reducing the infestation of A. japonicus and the optimal concentration of M. oleifera leaf extract in reducing the infestation of A. japonicus in goldfish (C. auratus). This study used a completely randomized design (CRD) with treatments namely control, 600 ppm, 700 ppm, 800 ppm and 900 ppm with four times of replication. The main parameter is the percentage decrease in infestation of A. japonicus. The results of the research data were analyzed by using ANOVA and followed with the Ducan Multiple Range Test. The results showed that Moringa leaf extract could reduce the infestation of A. japonicus. The optimal concentration of Moringa leaf extract in reducing the infestation of A. japonicus was obtained from a concentration of 700 ppm.
KORELASI KONFLIK INTRA ORGANISASI DAN KOMPENSASI PERUSAHAAN DENGAN PRESTASI KERJA KARYAWAN Subekti, Sri; Sulistyaningrum Indrawati, Cicilia Dyah; Widodo, Jumiyanto
Jurnal Informasi dan Komunikasi Administrasi Perkantoran Vol 2, No 1 (2013): Juni
Publisher : Pendidikan Administrasi Perkantoran FKIP Univesitas Sebelas Maret

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Abstract

The objectives of research are: (1) to find out whether or not there is a significant positive correlation between internal organization conflicts and work performance of the PT. Telkom Surakarta?s employees, (2) to find out whether or not there is a significant positive correlation between company compensation and work performance of the PT. Telkom Surakarta?s employees, and (3) to find out whether or not there is a significant positive correlation between internal organization conflict and company compensation with work performance of the PT. Telkom Surakarta?s employees.This study employed a quantitative research approach with a correlational descriptive method. The population of research was all employees of PT. Telkom Surakarta Office consisting of 96 employees. The sampling technique used in this research was random sampling one, taking 58 employees or 60% of population number. Techniques of collecting data used were questionnaire and documentation. The data analysis was done using a multiple linear regression analysis technique.Considering the result of research, it could be concluded that: (1) there was a positive significant correlation of Internal Organization Conflict variable (X1) and the Employee Work Performance in Human Resource (HR) department of PT. Telkom Surakarta, (2) there was a positive significant correlation of Company compensation variable (X2) and the Employee Work Performance in Human Resource (HR) department of PT. Telkom Surakarta, and (3) there was a positive significant correlation between Internal Organization Conflict (X1) and Company compensation (X2) variables simultaneously with the Employee Work Performance in Human Resource (HR) department of PT. Telkom Surakarta.The relative contribution of Internal Organizational Conflict (X1) to the employee work performance (Y) was 42.61%, the relative contribution of company compensation (X2) to the employee work performance (Y) was 57.39%, the effective contribution of Internal Organizational Conflict (X1) to the employee work performance (Y) was 19.70%, the effective contribution of company compensation (X2) to the employee work performance (Y) was 26.54%.Keywords: Internal Conflict Compensation, Employee Performance.
PENGELOLAAN MANGROVE SEBAGAI SALAH SATU KEANEKARAGAMAN BAHAN PANGAN Subekti, Sri
Prosiding SNST Fakultas Teknik Vol 1, No 1 (2012): PROSIDING SEMINAR NASIONAL SAINS DAN TEKNOLOGI 3 2012
Publisher : Prosiding SNST Fakultas Teknik

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Abstract

Mangrove merupakan vegetasi hutan yang tumbuh di antara pasang surut, tetapi juga dapat hidup pada pantai karang, pada dataran koral mati yang diatasnya ditimbuni selaput tipis pasir atau ditimbuni lumpur atau pantai berlumpur. Berbagai jenis mangrove terutama padabuahnya dapat digunakan sebagai bahan baku olahan pangan yang sat ini mulai berkembangdengan pesat. Mangrove jenis Pedada (Sonneratia), Brayo (Avicennia), Bakau (Rhizophora) dan Tancang (Bruguiera) menjadi sirup, onde-onde, klepon, resoles, kolak, dodol, bolu dan panganan lezat lainnya. Untuk mendukung keberlanjutan kawasan mangrove diperlukan suatuupaua pengelolaan mangrove yang berkelanjutan sehingga nantinya dapat digunakan oleh generasi sekarang dan generasi yang akan datang, Perlu juga diupayakan pengembangan danpenataan tata hijau di kawasan pesisir dengan pembangunan Green Belt (Sabuk Hijau).   Kata kunci: mangrove, buah, olahan pangan, pengelolaan
Utilization of Katuk (Sauropus androgynus L Merr) meal and extract as ration substitution to produce low chollesterol Japanese Quail product Subekti, Sri; Piliang, Wiranda G; Manalu, Wasmen; Murdiati, Tri Budhi
Indonesian Journal of Animal and Veterinary Sciences Vol 11, No 4 (2006)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (131.636 KB) | DOI: 10.14334/jitv.v11i4.534

Abstract

Katuk (Sauropus androgynus L Merr) is known to contain carotenoids, vitamin E, vitamin C, protein and phytosterol compound. This study was aimed to determine the ability of phytosterols in Sauropus androgynus (SA) leaf to obtain poultry product that has low cholesterol content. One hundred fifty female quails were raised from 2-27 weeks old, divided into three treatment diets, five replicates with 10 quails in each replicate. The treatment diets were: 1) Control group: diet without katuk leaf meal; 2) Diet with 9% SA ethanol 70% extract (SAE); 3) Diet containing 9% SA meal (SAM). Kolesterols were determined with CHOD-PAP-Method by Human, there is enzymatic Colorimetric Test for Cholesterol with Lipid Clearing Factor. The result showed that total cholesterol in the egg yolk, carcass and liver of SAE and SAM treated quails were lower (P&lt;0.05) than that of the control-treated quails, except the cholesterol content in the serum. These findings indicated that the cholesterol concentration of female quails was decreased due to phytosterol content in katuk leaf. Key Words: Phytosterol, Katuk Leaf, Kolesterol, Quails
Effect of Katuk leaf (Sauropus androgynus L. Merr) suplementation in the diet on reproductive function of Quail Subekti, Sri; Sumarti, Sri Susilogati; Murdiarti, Tri Budhi
Indonesian Journal of Animal and Veterinary Sciences Vol 13, No 3 (2008)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (174.277 KB) | DOI: 10.14334/jitv.v13i3.580

Abstract

Katuk (Sauropus androgynus L Merr) is known to contain carotenoids, vitamin E, vitamin C, protein, and sterol compounds. This study was aimed to determine whether phytosterol in Sauropus androgynus (SA) leaf affected the reproductive system of female quails. One hundred and fifty female quails were raised from 2-27 weeks old and divided into three dietary treatment, with five replicates and 10 quails in each replicate. The treatment diets were: 1) control group: diet without katuk leaf meal; 2) diet with 9% SA ethanol extract (TEK); 3) Diet containing 9% SA meal (TDK). The TDK-fed quails laid their first eggs at 46 day of age, compared with the TEK (52 day) and the control groups (53 day). The highest fertility (94.55%) and hatchability (93.29%) were obtained from the TDK fed quails at the age of 23 and 24 weeks, respectively. These findings indicated that the reproductive system of female quails were improved not only by phytosterol in SA leaf, but also by the β-carotene, vitamin C, α-tocopherol in SA leaf. Key Words: Katuk Leaf, Fertility, Hatchability, Quail
THE ROLE OF SOCIAL CAPITAL IN IMPROVING THE DYNAMICS OF FARMER GROUPS Sri Subekti; Mudiyono Mudiyono; Sri Peni Wastutiningsih
UNEJ e-Proceeding International Conference on Agribusiness Marketing (ICAM) 2012, Faculty of Agriculture, University o
Publisher : UPT Penerbitan Universitas Jember

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Abstract

Social capital is social energy in a community used to actuate a farmer group to be more dynamic to gain an end. Social capital cannot be formed by farmers individually, but it extremely depends on farmer group’s capacity and government to build trust, norms and social networks. Qualitative method is used in this research. The data collection is completed by using triangulation including non-participant observation, interview in depth,and documentation. The approach used is a case study that is to study the role of social capital in improving the dynamics of the Orange farmer group Sidomulyo IV, Sidomulyo village, Umbulsari subdistrict, Jember regency. The study results show that the members’ trust toward the farmer group Sidomulyo IV motivates their members to work together and to actively participate in the community’s activities to achieve their goals, so that their group becomes more dynamic. Cooperation creates the norms for the farmer group including the meeting schedule, the cash collection, the facility utilization, government help administration, and pest and disease management. It also builds social networks to fulfill the members’ needs involving the official kiosks of production utilities, gapoktan (farmer group alliance), government, Orange traders, and Hippa (association of waterusing farmers). Counseling activity through SL program (field school) also can increase farmer group’s social capital.
Potensi dan Pengaruh Batang Pisang Sebagai Media Filter Pada Pengolahan Air Limbah Pencucian Kendaraan Bermotor Kusumawardani, Yustika; Subekti, Sri; Soehartono, Soehartono
Jurnal Presipitasi : Media Komunikasi dan Pengembangan Teknik Lingkungan Vol 16, No 3 (2019): November 2019
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (304.716 KB) | DOI: 10.14710/presipitasi.v16i3.196-204

Abstract

Wastewater vehicle washing that discharged directly into water bodies can increase pollution levels. Wastewater treatment of small-scale vehicle washing businesses must be efficient, no need large areas, easy to operate and economical. The alternative technology is using simple wastewater filtration system. Banana stems can be used for filter media. Previous studies mention banana stems have cellulose content and have high hygroscopic ability. High cellulose content allows the potential to be used as an absorbent media. Hygroscopic properties are useful for absorbing harmful inorganic chemicals. The study was conducted by making a filtration reactor using banana stem filter media with a variation of residence time and filter media thickness. Sample testing by analyzing COD, TSS and Detergent parameters. The test results significant decrease in TSS and detergent parameters. The highest percentage decrease in TSS reached 91% and detergent 96%. COD decreased during the filtration treatment directly, but after that increased with the length of duration. This is due to biological influence and the high percentage of organic matter and biomass of banana stems. It can be concluded that the banana stem potential as a filter media for direct wastewater treatment and effective in reducing TSS and detergents for washing vehicles wastewater. 
Effect of Katuk leaf (Sauropus androgynus L. Merr) suplementation in the diet on reproductive function of Quail Sri Subekti; Sri Susilogati Sumarti; Tri Budhi Murdiarti
Jurnal Ilmu Ternak dan Veteriner Vol 13, No 3 (2008): SEPTEMBER 2008
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (174.277 KB) | DOI: 10.14334/jitv.v13i3.580

Abstract

Katuk (Sauropus androgynus L Merr) is known to contain carotenoids, vitamin E, vitamin C, protein, and sterol compounds. This study was aimed to determine whether phytosterol in Sauropus androgynus (SA) leaf affected the reproductive system of female quails. One hundred and fifty female quails were raised from 2-27 weeks old and divided into three dietary treatment, with five replicates and 10 quails in each replicate. The treatment diets were: 1) control group: diet without katuk leaf meal; 2) diet with 9% SA ethanol extract (TEK); 3) Diet containing 9% SA meal (TDK). The TDK-fed quails laid their first eggs at 46 day of age, compared with the TEK (52 day) and the control groups (53 day). The highest fertility (94.55%) and hatchability (93.29%) were obtained from the TDK fed quails at the age of 23 and 24 weeks, respectively. These findings indicated that the reproductive system of female quails were improved not only by phytosterol in SA leaf, but also by the β-carotene, vitamin C, α-tocopherol in SA leaf. Key Words: Katuk Leaf, Fertility, Hatchability, Quail
Pengetahuan Gizi dan Pola Asuh Ibu Anak Balita Gizi Kurang di Kelurahan Pasteur Kecamatan Sukajadi Bandung Subekti, Sri; Yulia, Cica
INVOTEC Vol 8, No 1 (2012)
Publisher : Faculty of Technological and Vocational Education-Universitas Pendidikan Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17509/invotec.v8i1.6110

Abstract

Nutrition knowledge is principal issues related to various nutritional problems that occur in Indonesia. Maternal role in children’s growth and development is very dominant, that is in caring for and educating them. Parenting patterns applied by the mother can affect the growth of the children, especially the nutritional status. Goals to be achieved in this study are: 1) to inquire nutrition and health knowledge of mothers with malnutrition children; 2) to examine food and health parenting pattern applied by mothers with malnutrition children and 3 ) to analyze the relationship between nutrition and health knowledge and parenting pattern implemented by the mother. The method used in this study is the Cross-Sectional. The results show that more than half of mothers’ nutrition and health knowledge is in medium category. More than half of mothers’ food parenting pattern is in fairly good category. Whereas, mothers’ health parenting pattern is in good category. Toddlers’ nutritional status, as measured using anthropometry, is generally in normal category. Spearman correlation test results state that there is a positive and significant relationship, and thus it can be concluded that the higher the mother's nutrition knowledge score, the better the food parenting pattern.
Co-Authors Achmad Amarudin Adam Sultoni Ade Fitri Noor Inaya Adelina Grace Aditya Gita Rohmatullah Agustina Agustina Agustono - Ahmad Syahrul Mubarak Akhmad Taufiq Mukti Aliffiansyah Rizky E Amadeus Michel Goein Annisa Nur R. P. Apriyanti, Eny Arini Arini Arya Witantama Aryati Aryati Berliana, Suci Wahyu Budi Utomo BUDI UTOMO Christine J K Ekawati Cicilia Dyah Sulistyaningrum Indrawati Damayanti, Wilda Ayu Devy Rahmawati Putri Diah Ayu Puspitarini Dr. Woro Hastuti Satyantini, Ir., M.Si Dwi Yuli Pujiastuti Eduardus Bimo Aksono Herupradoto Eka Pramyrtha Hestianah Ellis Endang Nikmawati Endang Dewi Masithah Eny Apriyati Fathmawati Fathmawati Feby Dwirahmadi Ferlyn Hendra Wiyatno Florentina Sustini Franch Dalahi Galih Candra Prakosa Yudha Gunanti Mahasri Gunanti Mahasri Hari B. Notobroto Hari Basuki Notobroto Harsasi Setyawati Hebert Adrianto Heny Arwati Herliana Arsianingtyas Hervina Benazir Ardiyanti Hurrul Fikri Ida Ayu Puspitasari Idha Kusumawati Iip Saripah, Iip Ike Nur Firdhayani Ilham Harlan Amarullah Indah S Tantular Jumiyanto Widodo Juniastuti Juniastuti katiah, katiah Khairul Bariyah Khoriri, Syaifurrizal Khuril Zaqyyah Kismiyati Kismiyati , Kris Cahyo Mulyatno Kusnoto Kusnoto Kusumawardani, Yustika Laksmi Sulmartiwi Lina Wafia Asmi Lindarto, Wira Widjaya Lucia Tri Suwanti, Lucia Tri Ludira Sari Mardiah Rahma Umami Mariana, Arni Retno Marina hasan Masanori Kameoka Mirni Lamid Mochammad Amin Alamsjah Mohamad Akmal A.H. Mohd Fazrul Hisam Abd Aziz Mohd Ihwan Zakariah Mudiyono Mudiyono Muhammad Aiman Yudarana Yudarana Muhammad Arief Muhammad Herman Mupita, Jonah Nathania Disa Ariesta Andriani Novi Krishatno Putera Novy Prasetya Nunuk Dyah Retno Lastuti Nur Anggraeni, Esti Mega Nur Fitriani Nurines Oktavia Ayuningtiaz Nurul Kumalasari Oktofianus Sila Patmawati Patmawati Pradiptya Ayu Harsita Puji Basuki Purwaningrum, Shintawati Dyah Puspa Wardhani R. Wahid Nur Yusuf Ragu Harming Kristina Ragu Harming Kristina Rahayu Kusdarwati Rani Frisca Christiana Rengga Eko Pradipta Renyta Andini, W. Ririh Yudhastuti Ririh Yudhastuti Ririh Yudhastuti Ririn Agustiya Riswanda, Andri Rizhar Eman Karunia Akbar Robi M.A Yahya Santi Martini Sari, Putri Desi Wulan Sariningrum, Nadya Nur Selvi Lely Rosanti Setyawati, Febri Shifa Fauziyah Shintawati Shintawati Shuhai Ueda Siti Churrotin Sitti R. Umniati Soegeng Soegijanto Soehartono Soehartono Sofia Sofia Sri Kiswati Sri Kiswati Sri Peni Wastutiningsih Sri Sumarsih Sri Susilogati Sumarti Subagyo Yotopranoto Sudarko Sudarko Sudarno Sudarno Suhartiningsih Dwi Nurcahyanti Sukarya Sukaryo Sukaryo Sukaryo SUPRIYONO . Surya Aditya Wicaksono Susiyanti, Rosi Tanzilia, May Fanny Tegar Putra Purnayudha Teguh Hari Sucipto Teguh Hari Sucipto Titin Agustina Tjahjaningsih, Wahju Tomohiro Kotaki Tri Budhi Murdiarti Tri Budhi Murdiati Tri Budhi Murdiati Tutik Juniastuti Ulia Fajriah Ummi Chasanah Wanti Wanti Wanti Wanti Wasmen Manalu Wilda Ayu Damayanti Wiranda G Piliang Wiranda G Piliang WIWIEK SRI WAHYUNI Yadi Ruyadi Yarin Dwi Monica Yoes Prijatna Dachlan Yulia, Cica Yuwono Marta Dinata