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Using homeopathica drugs combination at peripartal phase in preventing bovine subclinical mastitis Sanjaya, Agatha W.; Wibawan, I W.T.; Sudarwanto, Mirnawati; Widodo, Setyo; Enbergs, Heinrich
Medical Journal of Indonesia Vol 13, No 4 (2004): October-December
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (197.136 KB) | DOI: 10.13181/mji.v13i4.158

Abstract

A total of 33 dairy lactating cows suffering from subclinical mastitis were classified into group A (10 cows), group B (12 cows) and group C (11 cows). They were treated with the combination of homeopathic drugs and placebo, applied at the 4th and 3rd week antepartum (a.p), and continuing every week postpartum (p.p), for four times. Group A received Coenzyme comp® (in the 4th and 3rd week a.p), Lachesis comp® combined with Traumeel®(1st and 2nd week p.p) and Coenzyme comp® combined with Carduus comp® (3rd and 4th week p.p). Group B received Traumeel®+ Mucosa comp® (4th and 3rd week a.p), Lachesis comp®+ Traumeel® (1st and 2nd week p.p) and Coenzyme comp®+ Carduus comp® (3rd and 4th week p.p) and group C as a placebo. The incidence of subclinical mastitis in group A and C appeared irregular. In contrast, group B showed a constant percentage (33.3%). Group A and B showed significant response to the homeopathic drugs, expressed as an increasing of the somatic cell count value. At peripartal phase, haptoglobin increased in group B 0.80 mg/ml and group A as well as placebo 1.40 mg/ml. After calving, group B expressed a constant value (0.05 mg/ml), while group A and placebo rose significantly. The milk yield in normal lactation period (the 3rd - 7th month) increased significantly, with an increasing 14.1% for group B and 4.9% A respectively. (Med J Indones 2004; 13: 221-6)Keywords: Homeopathy, Homeopathic combination, Subclinical Mastitis, Peripartal Phase
Detection of Mycobacterium avium subspecies paratuberculosis in formula milk from Bogor using PCR IS 900 Nugroho, Widagdo S.; Sudarwanto, Mirnawati; Lukman, Denny W.; Naim, Rochman; Hassan, Abdulwahed A.; Usleber, Ewald
Medical Journal of Indonesia Vol 17, No 3 (2008): July-September
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (339.447 KB) | DOI: 10.13181/mji.v17i3.321

Abstract

Crohn’s disease (CD) that becomes a public health concern in developed countries shows similarities in clinical signs and pathological features with Johne’s disease (JD) in ruminants infected by Mycobacterium avium subspecies paratuberculosis (MAP). Few researches conducted in Europe, the USA, and Australia showed relationships between MAP, CD, JD and dairy products. Indonesians consume milk and diary products from domestic and imported source. Adji in 2004 found some domestic dairy cows that were seropositive for MAP, and this could be a serious problem in dairy farm animals and human health in the future. The aim of this study was to detect MAP in the growing up formula milk. Fifty samples from five established factories were taken from supermarkets in Bogor. Polymerase chain reaction method (PCR) with insertion sequence (IS) 900 as primer and culture in Herrold’s egg yolk media with mycobactin J (HEYM J) as a gold standard were used in this study. Neither MAP grew up in HEYM J medium after 20 weeks of culture period nor positive samples by PCR IS 900 were found. Although there were no positive samples found in this study, further extensive and comprehensive studies on MAP should be done with more and varied samples, as well as in human to provide data on MAP in Indonesia. (Med J Indones 2008; 17: 183-7)Keywords: Crohn’s disease, dairy cow, growing up formula milk
GEJALA KLINIS DAN PATOLOGI ANATOMI PASCA INFEKSI Campylobacter jejuni PADA AYAM BROILER [Clinical Symptoms and Pathology Anatomy due to Infection Campylobacter jejuni on Broilers] Andriani, Andriani; Sudarwanto, Mirnawati; Setiyaningsih, Surachmi; Kusumaningrum, Harsi Dewantari; Pisestyani, Herwin
BERITA BIOLOGI Vol 11, No 3 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (375.54 KB) | DOI: 10.14203/beritabiologi.v11i3.498

Abstract

Campylobacter spp. is a bacteria foodborne zoonotic agent that can infect humans and animals, especially birds. This bacteria can cause important problems in the public health since the beginning at the farm level, the level of retail and on the table. Poultry meat is the main source of contamination because the digestive tract of poultry is the predilection of Campylobacter jejuni. During this infection C. jejuni in poultry did not show typical clinical symptoms, making detection quite difficult to diagnose this disease at the farm level. Incidence infection of Campylobacter sp. in developed countries had 5-90%. Currently poultry production and consumption are predicted to still increase due to a high demand for low price protein. This research is conducted to determine the clinical and pathologic anatomy due to an infection of C. jejuni on chicken digestive tract. A total of 105-day-old chickens are divided into 3 groups, each consisting of 35 individuals. Group A was the control group (not infected) group B (infected with C. jejuni ATCC 33291), and group C (infected with field isolate of C. jejuni/C1). Infection of the chicken is done 0.5 ml peroral suspension concentration of104cfu/ml. Anova Duncan is used to analyze parameters of body weight, increase weight gain, feed consumption, FCR values. Kurskall Walis method used to analyze the parameters non-parametric form of the score changes in the macroscopic and microscopic intestinal and liver. Infection of C. jejuni changes the pathology anatomy of intestine i.e. hyperemia, enteritis and enteritis catharalis haemorrhagic. A microscopic change of intestinal is oedema,haemorrhage and infiltration of inflammatory cell. Changes of pathology anatomy liver are colour smeary, congesti, pale, fragile and necrotic. Microscopic changes of liver are degeneration and focal hepatic necrosis. Group observation infected by C. jejuni caused chicken cannot reach maximum body weight and can be detrimental to farmers because of increase of the value of the feed conversion ratio (FCR).
Profil Peternakan Babi Di Kota Kupang Dan Potensi Penularan Trichinellosis Angi, Andrijanto Hauferson; Satrija, Fadjar; Lukman, Denny Widaya; Sudarwanto, Mirnawati; Sudarnika, Etih
JURNAL KAJIAN VETERINER Vol 2 No 2 (2014): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v2i2.997

Abstract

Trichinellosis is a parasitic disease of humans caused by eating raw from domestic or game animals infected by Trichinella spp. Human trichinellosis contracted from commercial supplies of meat have been most often linked to infected pigs, wild boar, or horses. Trichinella is a nematode which has an atypical direct life cycle that does not involve stages developing outside of the host. This study was conducted to see the profile of pig farms in the city of Kupang and the potential transmission of trichinellosis. The data was derived from interview 60 farmers in 6 sub districts in city of Kupang by using a structured questionnaire and analyzed descriptively. The results of the questionnaire survey showed that many race of pig from a mixed race, the seeds come from traditional breeding. Feed rest of the home or restaurant are usually directly given to the pigs. The presence of rat in around of the cage often. All respondents were interviewed did not know or hear about trichinellosis disease which can be one cause of the spread of trichinellosis in city of Kupang.
BIOSINTESIS ANTIGEN PERMUKAAN HEPATITIS B “HBSAG100” PADA ESCHERICHIA COLI DALAM RANGKA PRODUKSI PROTEIN REKOMBINAN SEBAGAI MODEL IMUNOGEN UNTUK MENGHASILKAN ANTIBODI Riyadi, Slamet; RA Maheswari, Rarah; Sudarwanto, Mirnawati; RZ, Fransiska; Ali, Muhamad
Jurnal Kedokteran YARSI Vol 18, No 2 (2010): MEI - AGUSTUS 2010
Publisher : Lembaga Penelitian Universitas YARSI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (445.94 KB) | DOI: 10.33476/jky.v18i2.188

Abstract

Biosintesis protein rekombinan melalui Escherichia coli memberikan alternatif untuk menghasilkan protein antigen yang bermanfaan bagi kepentingan kesehatan yang bebas dari protein manusia. Penelitian ini menggabungkan fragmen DNA dari antigen permukaan virus Hepatitis B dengan gen penyandi enzim gluthation-S-transferase (GST) di dalam plasmid p GEX-4T-2 yang di ekspresikan di dalam sel-sel Escherichia coli. Polypeptida dengan berat molekul sekitar 34,8 kDa telah diproduksi dan diidentifikasi sebagai protein gabungan GST-HB100. Protein gabungan tersebut kemudian dimurnikan menggunakan kolum GSTrap yang disambung dengan kolum HiTrap. Selanjutnya, protein hasil pemurnian tersebut diharapkan bisa digunakan sebagai bahan vaksin atau untuk menghasilkan antibodi.
PENGETAHUAN, SIKAP DAN PERILAKU IBU TERHADAP KEAMANAN MAKANAN TAMBAHAN PEMULIHAN YANG DIKONSUMSI BALITA PENDERITA GIZI BURUK Purwanti, Maya; Sudarwanto, Mirnawati; Rahayu, Winiati P.; Sanjaya, A. Winny
Jurnal Penyuluhan Pertanian Vol 3 No 1 (2008)
Publisher : Politeknik Pembangunan Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (157.668 KB) | DOI: 10.51852/jpp.v3i1.239

Abstract

Penelitian ini bertujuan untuk melihat sejauh mana pengetahuan, sikap dan perilaku(PSP) ibu penerima bantuan Makanan Tambahan Pemulihan (MT-P) dalam menjaga keamananMT-P yang dikonsumsi balita, dan melihat asosiasi berbagai kondisi yang mempengaruhi PSPibu. Responden yang diambil sebanyak 50 orang ibu yang ditentukan secara proporsionalberasal dari 10 Puskesmas di Kabupaten Bogor. Berdasarkan analisis data dan mikrobiologiksampel diketahui bahwa pengetahuan responden tentang tatacara menjaga keamanan MT-Pyang dikonsumsi balita dipengaruhi oleh pendidikan dasar (r=0,324) dan beban anak yangdimiliki (r=0,364). Sikap peduli responden terhadap keamanan MT-P dipengaruhi olehpengetahuan tentang tatacara preparasi dan penyimpanan MT-P (r=0,436) dan beban anak yangdimiliki (r=-0,378). Perilaku responden dalam menjaga keamanan MT-P dipengaruhi olehpengetahuan tentang tatacara preparasi dan penyimpanan MT-P (r=0,392) dan jumlah uangyang dikelola responden setiap bulannya (r=0,409). Perilaku yang tidak hati-hati dariresponden menyebabkan jumlah mikroba aerob di dalam MT-P terlarut melebihi batas SNI danmunculnya bakteri patogen seperti Bacillus cereus dan Clostridium perfringens. Hasilpenelitian menunjukkan bahwa pemahaman ibu-ibu yang mendapat bantuan MT-P masihrendah dalam menjaga keamanan MT-P yang dikonsumsi balita.