I Made Sudiana
Research Centre for Biology, the Indonesian Institute of Sciences (LIPI), Cibinong Science Centre Jalan Raya Jakarta-Bogor km 46, Cibinong 16911, Indonesia

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THE PHSYIOLOGICAL CHARACTER OF BACTERIA ISOLATED FROM BANANA’S RHIZOSPHERE FROM MALAKA, EAST NUSA TENGGARA, AND THEIR ROLE ON PLANT GROWTH PROMOTION ON MARGINAL LAND Napitupulu, Toga Pangihotan; Kanti, Atit; Sudiana, I Made
BERITA BIOLOGI Vol 18, No 3 (2019)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v18i3.3748

Abstract

The objective of the study was to isolate microorganism that can produce growth hormone, fix atmospheric nitrogen, and solubilize inorganic phosphate that may be used for enhancing the growth of banana in marginal land.  A total of 25 bacteria associated with banana that belonged to six genera were isolated from namely Acinetobacter, Enterobacter, Pantoea, Klebsiella, Rhizobium, and Cupriavidus genera. Gammaproteobacteria is the dominant class, followed by Betaproteobacteria and Alphaproteobacteria. All isolates were screened for multiple plant growth promoting traits which may play a role in banana growth, namely fixing nitrogen, solubilizing phosphate, and producing Indole Acetic Acid (IAA). Twenty-two isolates were capable to fix nitrogen, 21 isolates can solubilize insoluble phosphate, and 15 isolates produced IAA dependent of L-Tryptophan presence. Despite most of the bacteria isolates exhibited one plant growth-promoting activities, Enterobacter and Klebsiella genera showed three of plant growth promoting bacteria traits. 
Biodegradasi Alkil Benzena Sulfonat oleh Psedomonas cepacia Sudiana, I Made
JURNAL BIOLOGI INDONESIA Vol 3, No 3 (2002): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (217.629 KB) | DOI: 10.14203/jbi.v3i3.3469

Abstract

ABSTRACTAlkyl Benzene Sulfonate Biodegradation of Psedomonas cepacia. Alkyl Benzene Sulfonate (ABS), naturally slow biodegradable substances, and toxic to human, animal and microorganisms, is a focus of environmental studies. Microorganisms appeared to play important role on biodegradation of that substances in nature, and wastewater treatment procesess. S2 isolated from detergent contaminated soil was able to grow in media with ABS as the sole carbon source. ABS degradation took place under aerobic condition, at pH 4, temperature 30ºC with ?max of 0.0591-h, Ks = 3.25 mg/L, Vmax = 0.16 mg/L.hours-1, and Km = 14.52 mg/L. Analyses of 16s rDNA revealed that S2 is belonging to Pseudomonas cepacia.Key words: Alkyl Benzene Sulfononate (ABS), Pseudomonas cepacia, detergent
C0-CULTURE OF AMYLOLYTIC FUNGI Aspergillus niger AND OLEAGINOUS YEAST Candida orthopsilosis ON CASSAVA WASTE FOR LIPID ACCUMULATION [Akumulasi lipid oleh kultur campuran kapang Aspergillus niger dan khamir Candida orthopsilosis pada media limbah singkong] Kanti, Atit; Sudiana, I Made
BERITA BIOLOGI Vol 16, No 2 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3452.972 KB) | DOI: 10.14203/beritabiologi.v16i2.2207

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The objective of study was to evaluate the effectiveness of co-culture between amylolytic fungi  Aspergillus niger and lipid accumulating yeast Candida orthopsilosis for lipid accumulation on cassava waste. When grown in 5 % starch medium, all microbes was able to grow, but best growth was observed on Aspergillus niger, other microbes grew slower. Moderate growth was observed  on Saccharomycopsis fibuliger.  The two isolates were then selected for further studies. Aspergillus niger and Saccharomycopsis fibuliger were amylolytic microbes. The amylolytic activities were affected by temperature, pH and nitrogen sources.  Optimum temperature and pH for enzyme production were 30°C and 7.0 respectively. Both yeast extract and sodium nitrate were good nitrogen sources for amylase production. On cassava waste, the highest biomass and total lipid content were obtained by co-culture of Aspergillus niger and lipid accumulating yeast Candida orthopsilosis. Major lipid composition was oleic acids and strearic acids. The ability of co-culture of Aspergillus niger and lipid accumulating yeast Candida orthopsilosis grew and accumulated lipid on cassava waste would suggest that these culture were candidate for biofuel production.
PENERAPAN BIOLOGI TERAPAN OLEH SISWA SMP DI KABUPATEN TABANAN Surata, I Ketut; Sudirgayasa, I Gede; Sudiana, I Made; Marhaeni, I Gusti Agung Ayu Nova Dwi
Jurnal Santiaji Pendidikan (JSP) Vol 8 No 1 (2018): Jurnal Santiaji Pendidikan (JSP)
Publisher : Fakultas Keguruan dan Ilmu Pendidikan (FKIP) Universitas Mahasaraswati Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (201.56 KB) | DOI: 10.36733/jsp.v8i1.156

Abstract

This study aims to determine the application of applied biology in everyday life by junior high school students in Tabanan District of Bali Province. The method used in this research is survey research method. Respondents who are the subject of this research are grade VIII students from 3 sample schools in Tabanan District. The three sample schools are Public Junior High School 2 Tabanan, Public Junior High School 1 Kerambitan, and Public Junior High School 2 Penebel. Data collection was done by distributing questionnaires to respondents. The questionnaire consists of 25 question items. The collected data is analyzed descriptively by presentation in the form of a complete description with visual data in the form of tables and graphs. Each question item is given a range of 0 to 5. The minimum score is 0, whereas the maximum score is 5. The total score of each student will be converted to a scale of 100. Next is determined the mean-value for each sample school. The mean grade of each sample school was then compared and classified. Categorization of the average value refers to the classification of absolute norms scale 5. Based on the results of research can be concluded that the application of applied biology by junior high school students in Tabanan District is still quite enough. This condition requires all of us who are work in the education sector to find solutions through further studies to a better level. Thus, the expectations of what students have learned in the field of biology are useful to them and their environment.
DINAMIKA POPULASI MIKROBA BENTUK FILAMEN PADA PROSES ANAEROBIK-AEROBIK UNTUK PENAMBATAN POSPAT DENGAN MIKROBA [Dynamic Population of Filamentous Organism in Anaerobic-Aerobic Process for Enhanced Biological Phosphate Removal Process] Sudiana, I Made
BERITA BIOLOGI Vol 4, No 5 (1999)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v4i5.1252

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Filamentous bulking is commonly observed in activated sludge treatment plant. The present study is intended to study the dynamic of filamentous organism in enhanced biological phosphorous removal. An anaerobic-aerobic sequential batch reactor was operated at 6 cycles per day with anaerobic phase for 55 minutes, aerobic phase for 135 minutes and settling phase for 60 minutes. Population of filamentous organism were counted and identified.Fluorescence in situ hybridization (FISH) with oligonucleotide probe specific for the alpha, beta, gamma sub class Proteobacteria and gram positive high GC DNA content organism was done to identify in-situ community of filamentous organism in the activated sludge. Them were 4 major filamentous organisms namely Type 021N, 0041, Micmthrix oarvicella and Haliscomenobacter hydrosis, in which type 021N was always dominant. Deterioration of enhanced biological phosphorous removal process was observed when filamentous organism outgrew. Probing technique with oligonucleotide probe revealed that filamentous organism mostly belong to the gamma sub class of Proteobacteria.
AKTIVITAS CMC-ase KHAMIR Candida sp. YANG DIISOLASI DARI TANAH KEBUN BIOLOGI WAMENA, PAPUA Kanti, Atit; Sudiana, I Made
BERITA BIOLOGI Vol 6, No 5 (2003)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v6i5.1190

Abstract

Cellulolytic Candida sp.was isolated from Wamena, Papua.The strain was able to grow in media with carboxymethyl cellulose as a sole carbon source implying that the isolate produced 1-3 oc endo-gluconase.To study the effect of glucose and osmotic pressure on 0.1% glucose and 0.1% NaCl were augmented to CMC-contained media.Glucose augmentation affects cellulolytic activity of culture,probably due to higher biomass production in media. NaCl addition appear not to affect cellulolytic ability. Profile of pH also varied depend on the cultivation media. Maximum growth rate was achieved when glucose was added which was implying that glucose stimulate cell growth.
Production of Acid Phosphatase in Bacillus sp. Isolated from Forest Soil of Gunung Salak National Park Rahmansyah, Maman; Sudiana, I Made
JURNAL BIOLOGI INDONESIA Vol 6, No 3 (2010): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v6i3.3140

Abstract

ABSTRAKProduktivitas Fosfatase Asam pada Bacillus sp. yang Diisolasi dari Tanah Hutan TamanNasional Gunung Salak. Pada pengamatan ini dilakukan karakterisrik bakteri pelarut fosfatyang diisolasi dari tanah hutan Taman Nasional Gunung Salak. Sebanyak 21 koloni hasil isolasidiuji terhadap produktivitas enzim fosfatase berdasar pelarutan media mengandung fosfat.Isolat yang terkuat melarutkan fosfat diidentifikasi sebagai Bacillus sp. Pada pengamatanlanjutan terhadap strain teruji dilakukan penumbuhan pada media cair selama 90 jam inkubasi,dan hasilnya ternyata mampu melarutkan fosfat inorganik (Pi) dari sumber trikalsium fosfat(Ca-Pi) dan alumunium fosfat (Al-Pi) masing-masing pada kisaran 1,2 sampai 152 dan 0.8 sampai25 mg.L-1; dan menunjukkan aktifitas enzim fosfomonoesterase antara 0.2 sampai 1.01 unitpada media yang mengandung larutan para-nitrophenylphosphate sebagai media fosfatorganik (Po) artifisial. Konsumsi glukosa pada media yang diukur selama pertumbuhan sejalanpula dengan produk ortofosfat sebagai akibat adanya aktifitas enzim fosfatase. Peningkatanfosfatase juga sejalan dengan bertambahnya biomassa sel bakteri dan penambahan produkasam glukonat. Penurunan pH dari 7 menjadi 5 diakibatkan peningkatan produk asam glukonatdi dalam media tumbuh. Bakteri pelarut fosfat yang berasal dari tanah hutan Taman NasionalGunung Salak dapat memproduksi fosfatase asam untuk memineralisasi sumber-sumber fosfatmenjadi sumber nutrisi yang siap digunakan oleh akar tumbuhan, dan itu merupakan prediksikuat untuk menjadikan isolat bakteri pelarut fosfat sebagai sumber bahan pupuk hayati.Kata kunci: Bacillus sp., tanah hutan, fosfatase asam, Ca-Pi, Al-Pi.
Ragam Aktivitas Urease dan Fosfomonoesterase serta Perannya dalam Ketersediaan Nutrisi N dan P pada Tanah Kebun Biologi Wamena Rahmansyah, Maman; Latupapua, H.J.D.; Sudiana, I Made
JURNAL BIOLOGI INDONESIA Vol 3, No 4 (2002): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (890.513 KB) | DOI: 10.14203/jbi.v3i4.3325

Abstract

ABSTRACTDiscrepancy of urease and phosphomonoesterase activities and its role in establishing Nand P nutrition in soil collected from Wamena Biological Research Station. Microbialactivities in soil lead to know for establishing soil nutrient status. Accordingly, soil collectedfrom Biological Research Station in Wamena then sent to the laboratory and determined on itsenzymatic activities and the physicochemical, as well. In this work, the enzymatic activities ofurease and phosphomonoesterase were examined in relation with soil microbial respiration, inorder to understand the mineralization of nitrogenous and phosphorus compound in soil. Soilrespiration rate (2.43-3.21 mg C02 g-dm12hour) designated variation in each sample, as wellas urease (8.6-23.5 unit/g soil) and phosphomonoesterase (5.5-7.9 unit/g soil) activities.Phosphomonoesterase activity showed strong correlation with respiration rate within soil; andreveal to the configuration of the bioactivities and physicochemical soil figures concluded thatthe B sample has the poor fertility. The phenomenon of data fulfill that bioactivities hadcorrelation with the physicochemical compound in the soil.Keywords: respiration, urease, phosphomonoesterase, Wamena Biological Research Station.
PENGARUH APLIKASI HIDROGEL DAN KOMPOS TERHADAP RETENSI AIR DAN PERTUMBUHAN TANAMAN SORGUM PADA ULTISOL Nugroho, Muhamad Slamet; Prijono, Sugeng; Sudiana, I Made
Jurnal Tanah dan Sumberdaya Lahan Vol 5, No 1 (2018)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (197.821 KB)

Abstract

Ultisol has several constraints in the management and utilization for crop production such as erosion, easy leaching, low water retention, low cation exchange capacity and low nutrients. Provision of soil conditioner in the form of hydrogel and compost can increase water retention and improve some properties and nutrient content on the soil to increase plant growth. This study was conducted in a greenhouse of the Biology Research Center LIPI Cibinong, using a factorial randomized complete design with three replications in two experimental units, i.e. soil incubation and soil planted with Sorghum bicolor Super 2. Treatments tested consisted of a combination of hydrogel treatments with doses of 0,5 g kg-1, 1 g kg-1, 2 g kg-1, and 4 g kg-1 of hydrogels and compost treatmenst with doses  0 g kg-1, 30 g kg-1, and 60 g kg-1. The results showed that the combination of hydrogel and compost significantly affected soil characteristics and increased the growth of sorghum compared with control. However, interactions  between the treatment of hydrogels and compost were only observed for permanent wilting point, water content of field capacity and pore drainage. In general, the combination of hydrogel and compost treatment with the highest dose of H4K2 (4 g kg-1 hydrogels and compost 60 g kg-1) had the best effect on increasing water retention and sorghum plant growth on Ultisol.
PENGARUH ZEOLIT DAN KOMPOS TERHADAP RETENSI AIR, KAPASITAS TUKAR KATION, DAN PERTUMBUHAN TANAMAN SORGUM (Sorghum bicolor (L.) Moench) PADA ULTISOL Balqies, Sayyida Camilla; Prijono, Sugeng; Sudiana, I Made
Jurnal Tanah dan Sumberdaya Lahan Vol 5, No 1 (2018)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (310.149 KB)

Abstract

Ultisol is one of soils type in Indonesia which has several problemsr for the cultivation of plants such as low pH, high Al saturation, low content of organic matter, and limited water storage. The objective of this study was to investigate the effect of zeolite and compost application on water retention, cation exchange capacity, and growth of sorghum at an Ultisol. The study was conducted using a completely randomized factorial design with two factors, the first factor was zeolite doses with five levels consisting of Z0 (no zeolite), Z1 (20 t ha-1), Z2 (40 t ha-1), Z3 60 t ha-1), Z4 (80 t ha-1) and the second factor is variation of compost dosage with three levels consisting of K0 (no compost), K1 (compost 3%), K2 (compost 6%) Each treatment was repeated three times. The results showed that in the treatment of zeolite 80 t ha-1 water retention increased by 13,7% and in the treatment of compost 6% reached 13,76% but there was no interaction between zeolite and compost to water retention. The increase of water retention was due to the increase in C-organic and porosity and the decrease of soil bulk density. The application of zeolite and compost succeeded in increasing the cation exchange capacity at the zeolite treatment of 80 t ha-1 + 6% compost to 17,46 cmol kg-1 due to the increase of soil pH. If zeolite and compost were given in high doses they could increase the growth of sorghum.