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All Journal Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Santiaji Pendidikan (JSP) BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Jurnal Kesehatan Bakti Tunas Husada Jurnal Ilmiah Pendidikan dan Pembelajaran Journal of Education Technology JIIP (Jurnal Ilmiah Ilmu Pendidikan)
I Made Sudiana
Research Centre for Biology, the Indonesian Institute of Sciences (LIPI), Cibinong Science Centre Jalan Raya Jakarta-Bogor km 46, Cibinong 16911, Indonesia
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Education Health Professions Mathematics Public Health Other

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THE COMMUNITY OF SOIL YEASTS IN GUNUNG HALIMUN NATIONAL PARK Kanti, Atit; Sudiana, I Made; Julistiono, Heddy
BERITA BIOLOGI Vol 5, No 6 (2001)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (409.796 KB) | DOI: 10.14203/beritabiologi.v5i6.1081

Abstract

Fifty-two isolates were isolated from Gunung Halimun National Park on the basis of morphological and some physiological characteristics.Those isolates were belonged to three groups namely, ascomycetous, basidiomycetous and imperfect yeasts.Rhodotolum sp.was only found in Ciptarasa site at 1500 m asl, ascomycetous yeasts was only isolated from deteoretic root in Gunung Botol site, while Candida sp.(small globose shaped cells) was only isolated from soil at 1800 m asl of Gunung Botol site. Type of plant species appeared has no effect on yeasts diversity as shown by similar yeasts diversity was observed at rhizosphere soil of three dominating plant (Schima waallichii, Castanopsis javanica and Altingia excelsa) at Cikaniki study site.
PHOSPHATASE ACTIVITY OF Bacillus sp. ISOLATED FROM FOREST SOIL OF GUNUNG HALIMUN NATIONAL PARK*[Aktifitas Fosfatase Bacillus sp. yang Diisolasi dari Tanah Hutan di Taman Nasional Gunung Halimun] Sudiana, I Made
BERITA BIOLOGI Vol 6, No 1 (2002)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (354.058 KB) | DOI: 10.14203/beritabiologi.v6i1.1169

Abstract

Bacillus sp diisolasi dari tanah Taman Nasional Gunung Halimun. Dalam media tumbuh yang mengandung Ca3(PO4)2 bakteri membentuk zona bening disekitar koloni.Glukosa digunakan dengan cepat selama kultivasi.Tinggi konsentrasi fosfat terlarut yang dibebaskan selama fase pertumbuhan menunjukkan bahwa bakteri mampu memacu pelarutan Ca3(PO4)2. Selama fase pertumbuhan terjadi hidrolisa organik fosfat (phenyl phosphate) menghasilkan phenol dan fosfat hal ini menunjukkan,bahwa Bacillus sp mampu menggunakan organik fosfat.Selama kultivasi terjadi penurunan pH sejalan dengan pelarutan Ca3(PO4)2.
CELLULOLYTIC BACTERIA OF SOIL OF GUNUNG HALIMUN NATIONAL PARK Sudiana, I Made; Rahayu, Ritai Dwi; Imanuddin, Hartati; Rahmansyah, Maman
BERITA BIOLOGI Vol 5, No 6 (2001)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v5i6.1077

Abstract

The population of aerobic cellulolyitic bacteria (ACB) of soil Gunung Halimun National park and its celulolytic capacity were studied.The soil samples were collected from various altitude (500-1500) m asl.Microbial isolation was performed by culture enrichment technique with CMC (carboxymethyl cellulose) as the major carbon sources.The quantitative determination of ACB was performed by growing the microbes on CMC containing media, and utilizing congored as an indicator.ACB was indicated by formation of clearing zone surrounding growing colony.Cellulytic capacity of each isolates was determined by analysing the ratio of colony and clear zone formation.ACB were quite heterogenous include Bacillus sp., Clostridium sp., Chromobacterlum sp., Enterobacter sp., Moraxella sp. and Pseudomonas sp.
Increase of Citric Acid Production by Aspergillus niger InaCC F539 in Sorghum’s Juice Medium Amended with Methanol Kanti, Atit; Ilyas, Muhammad; Sudiana, I Made
JURNAL BIOLOGI INDONESIA Vol 14, No 2 (2018): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v14i2.3733

Abstract

ABSTRACTCitric acid demand increases steadily, and there is a need to increase productivity through selection of suitable carbon sources, and addition of substances that increase citric acids production rate. Methanol has been suggested to increase citric acid fermentation on high carbohydrate containing substances. The objective of the study was to evaluate the suitability of sweet sorghum juice for citric acids production and to verify the effect of methanol on citric acids production using Aspergillus niger InaCC F539  as inoculant. Sweet sorghum juice with the total initial reducing sugar of 11.5 % (w/v) was used as the sole carbon sources. To study the effect of total initial reducing sugar on citric acid production the initial reducing sugar was adjusted to the concentration of  30 to 75 g/L. Preliminary experiment was conducted to get the optimum methanol concentration that stimulate citric acid production. The optimum methanol concentration that stimulate citric acid production was 4% (v/v). Submerged fermentation was conducted as shake culture (125 rpm at 28 °C).  Citric acids production was affected by total initial reducing sugar. Higher total initial reducing sugar produced higher citric acids. Maximum citric acid production was 18.96g/L on sweet sorghum juice with 75 g/L total initial reducing sugar. Methanol 4 % (v/v) increase citric acid production by 41.35 to 65.89 %. Juice of sweet sorghum was a good medium for citric acids production, and methanol stimulate and increase citric acid production. It is a good basis for exploring efficient and cost effective industrial scale citric acid production. Keywords: Citric acid, Methanol, Sweet sorghum, Aspergillus niger 
Identification of Ectomycorrhiza-Associated Fungi and Their Ability in Phosphate Solubilization Mujahidah, Shofia; Sukarno, Nampiah; Kanti, Atit; Sudiana, I Made
JURNAL BIOLOGI INDONESIA Vol 14, No 2 (2018): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v14i2.3741

Abstract

ABSTRACTThe existence of Pinus sp. is very dependent on ectomycorrhizal (ECM) fungi. ECM fungi affect the growth of their hosts especially by increasing mineral availability and water intake. However ECM fungi is not the only one that plays a role in the growth of their host. There are many ECM-associated fungi which also have many important roles in the growth of the host. Helotiales which were isolated from the ECM of Pinus merkusii are known as the most member of root associated fungi. Three isolated Helotiales identified as Scytalidium sp., Helotiales sp., and Glutinomyces sp. by morphological and molecular identification based on ITS1, 5.8S rRNA, ITS2 DNAr region. All three isolates have the ability to solubilize phosphate. Compared with C. geophilum which already known as P solubilizing fungi, Glutinomyces solubilized 16.6 ppm P which is higher than C. geophilum which solubilized as much as 13.68 ppm in Pikosvkaya medium with glucose as carbon source and rock phosphate as phosphate source. Then followed by Scytalidum sp. and lastly Helotiales sp. Rock phosphate tend to harder to solubilize because its complex chemical form with other minerals. Keyword: ECM-associated fungi, Helotiales, phosphate solubilizing ability, Pinus merkusii 
Isolasi Bakteri Pengakumulasi Poliposfat Sudiana, I Made
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 9, No 2 (2004): June 2004
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (208.776 KB) | DOI: 10.24002/biota.v9i2.2898

Abstract

Polyphosphate accumulating bacteria play  important key role on biological phosphorous removal. Activated sludge originated from wastewater treatment plant for drinking water production acclimated with anaerobic-aerobic phase in sequential batch reactor continuously succeed to establish microbial community that are able to adsorb glucose and acetate under anaerobic condition.  Acinetobacter calcoaceticus S1 was an isolated bacterium from community performing organic substrate absorption. S1 is able to adsorb glucose and acetate under anaerobic condition. During substrate absorption an increase of orthophosphate in bulk solution indicating phosphate released out of cell. Reduction of glycogen and polyhydroxybutirate (PHB) took place under anaerobic phase. S1 is a community member of anaerobic-aerobic system for removal of phosphorous in influent wastewater.
THE PHSYIOLOGICAL CHARACTER OF BACTERIA ISOLATED FROM BANANA’S RHIZOSPHERE FROM MALAKA, EAST NUSA TENGGARA, AND THEIR ROLE ON PLANT GROWTH PROMOTION ON MARGINAL LAND Napitupulu, Toga Pangihotan; Kanti, Atit; Sudiana, I Made
BERITA BIOLOGI Vol 18, No 3 (2019)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v18i3.3748

Abstract

The objective of the study was to isolate microorganism that can produce growth hormone, fix atmospheric nitrogen, and solubilize inorganic phosphate that may be used for enhancing the growth of banana in marginal land.  A total of 25 bacteria associated with banana that belonged to six genera were isolated from namely Acinetobacter, Enterobacter, Pantoea, Klebsiella, Rhizobium, and Cupriavidus genera. Gammaproteobacteria is the dominant class, followed by Betaproteobacteria and Alphaproteobacteria. All isolates were screened for multiple plant growth promoting traits which may play a role in banana growth, namely fixing nitrogen, solubilizing phosphate, and producing Indole Acetic Acid (IAA). Twenty-two isolates were capable to fix nitrogen, 21 isolates can solubilize insoluble phosphate, and 15 isolates produced IAA dependent of L-Tryptophan presence. Despite most of the bacteria isolates exhibited one plant growth-promoting activities, Enterobacter and Klebsiella genera showed three of plant growth promoting bacteria traits. 
ANALISIS CEMARAN BAKTERI COLIFORM DAN ESCHERICIA COLI PADA DEPOT AIR MINUM ISI ULANG (DAMIU) Sudiana, I made
Jurnal Kesehatan Bakti Tunas Husada: Jurnal Ilmu-ilmu Keperawatan, Analis Kesehatan dan Farmasi Vol 20, No 1 (2020)
Publisher : STIKes Bakti Tunas Husada Tasikmalaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36465/jkbth.v20i1.553

Abstract

Sejalan dengan kemajuan teknologi dan diiringi dengan semakin padatnya aktivitas manusia maka masyarakat cenderung memilih cara yang lebih praktis dan biaya relatif murah dalam memenuhi kebutuhan air minum yaitu dengan mengonsumsi air minum isi ulang. Agar air minum isi ulang aman untuk dikonsumsi oleh masyarakat di Kecamatan Tabanan, maka perlu dilakukan uji kualitas apakah kandungan dalam air minum isi ulang sudah memenuhi Keputusan Menteri Kesehatan No. 907/MENKES/SK/VII/2002 Tentang Syarat dan Pengawasan Kualitas Air. Metode yang digunakan adalah sampling area yaitu peneliti melakukan  survei berdasarkan area kepadatan penduduk dan dipilih 2 depot air minum isi ulang sebagai sampel. Parameter yang diuji meliputi nilai MPN bakteri Coliform dan E. coli. Hasil uji laboratorium menyatakan nilai MPN Coliform dan E. coli pada kedua depot air minum isi ulang belum memenuhi persyaratan kualitas air minum dengan nilai MPN Coliform sebesar 93/100 ml dan E. coli pada sampel A sebesar 9/100 ml.
PENERAPAN BIOLOGI TERAPAN OLEH SISWA SMP DI KABUPATEN TABANAN Surata, I Ketut; Sudirgayasa, I Gede; Sudiana, I Made; Marhaeni, I Gusti Agung Ayu Nova Dwi
Jurnal Santiaji Pendidikan (JSP) Vol 8 No 1 (2018): Jurnal Santiaji Pendidikan (JSP)
Publisher : Fakultas Keguruan dan Ilmu Pendidikan (FKIP) Universitas Mahasaraswati Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (201.56 KB) | DOI: 10.36733/jsp.v8i1.156

Abstract

This study aims to determine the application of applied biology in everyday life by junior high school students in Tabanan District of Bali Province. The method used in this research is survey research method. Respondents who are the subject of this research are grade VIII students from 3 sample schools in Tabanan District. The three sample schools are Public Junior High School 2 Tabanan, Public Junior High School 1 Kerambitan, and Public Junior High School 2 Penebel. Data collection was done by distributing questionnaires to respondents. The questionnaire consists of 25 question items. The collected data is analyzed descriptively by presentation in the form of a complete description with visual data in the form of tables and graphs. Each question item is given a range of 0 to 5. The minimum score is 0, whereas the maximum score is 5. The total score of each student will be converted to a scale of 100. Next is determined the mean-value for each sample school. The mean grade of each sample school was then compared and classified. Categorization of the average value refers to the classification of absolute norms scale 5. Based on the results of research can be concluded that the application of applied biology by junior high school students in Tabanan District is still quite enough. This condition requires all of us who are work in the education sector to find solutions through further studies to a better level. Thus, the expectations of what students have learned in the field of biology are useful to them and their environment.
RAGAM AKTIVITAS UREASE DAN FOSFOMONOESTERASE SERTA PERANNYA DALAM KETERSEDIAAN NUTRISI N DAN P PADA TANAH KEBUN BIOLOGI WAMENA Rahmansyah, Maman; Latupapua, H.J.D.; Sudiana, I Made
JURNAL BIOLOGI INDONESIA Vol 3, No 4 (2002): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v3i4.3325

Abstract

ABSTRACTDiscrepancy of urease and phosphomonoesterase activities and its role in establishing Nand P nutrition in soil collected from Wamena Biological Research Station. Microbialactivities in soil lead to know for establishing soil nutrient status. Accordingly, soil collectedfrom Biological Research Station in Wamena then sent to the laboratory and determined on itsenzymatic activities and the physicochemical, as well. In this work, the enzymatic activities ofurease and phosphomonoesterase were examined in relation with soil microbial respiration, inorder to understand the mineralization of nitrogenous and phosphorus compound in soil. Soilrespiration rate (2.43-3.21 mg C02 g-'dm12hour) designated variation in each sample, as wellas urease (8.6-23.5 unit/g soil) and phosphomonoesterase (5.5-7.9 unit/g soil) activities.Phosphomonoesterase activity showed strong correlation with respiration rate within soil; andreveal to the configuration of the bioactivities and physicochemical soil figures concluded thatthe B sample has the poor fertility. The phenomenon of data fulfill that bioactivities hadcorrelation with the physicochemical compound in the soil.Keywords: respiration, urease, phosphomonoesterase, Wamena Biological Research Station.
Co-Authors Atit Kanti HEDDY JULISTIONO I Gede Sudirgayasa I Ketut Surata I Made Maduriana I Wayan Gata, I Wayan Imanuddin, Hartati LATUPAPUA, H.J.D. Latupapua, H.J.D. Maman Rahmansyah Marhaeni, I Gst Agung Ayu Nova Dwi Marhaeni, I Gusti Agung Ayu Nova Dwi Muhammad Ilyas Mujahidah, Shofia Mujahidah, Shofia Nampiah Sukarno Napitupulu, Toga Pangihotan Rahayu, Ritai Dwi