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All Journal AGRITROP Prosiding Seminar Biologi JURNAL TEKNOLOGI LINGKUNGAN AL KAUNIYAH BERKALA SAINSTEK Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Industrial and Systems Engineering Assessment Journal (INASEA) Agrointek Indonesian Journal of Agricultural Science Jurnal AgroBiogen Buletin Penelitian Tanaman Rempah dan Obat UNEJ e-Proceeding Extension Course Filsafat ( ECF ) Agriprima : Journal of Applied Agricultural Sciences Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal ASET (Akuntansi Riset) ANNALES BOGORIENSES Mesin Agritrop : Jurnal Ilmu-Ilmu Pertanian (Journal of Agricultural Science) J-Dinamika: Jurnal Pengabdian Kepada Masyarakat Buletin Penelitian Tanaman Rempah dan Obat Proceeding Biology Education Conference BIOVALENTIA: Biological Research Journal

Bambang Sugiharto

Fakultas Matematika Dan Ilmu Pengetahuan Alam Universitas Jember

Author-ID : 388822

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Physics Public Health Social Sciences

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APLIKASI SIMULASI UNTUK PERAMALAN PERMINTAAN DAN PENGELOLAAN PERSEDIAAN YANG BERSIFAT PROBABILISTIK Sugiharto, Bambang
Industrial and Systems Engineering Assessment Journal (INASEA) Vol 8, No 2 (2007): INASEA Vol. 08 No.2
Publisher : Industrial and Systems Engineering Assessment Journal (INASEA)

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One of the important aspect on plan and production control is the management ofÂ inventory. However, a good inventory management is easily implemented, particularlyÂ when it involves some probabilistic parameter. There are two important parameters thatÂ usually behave probabilistically, those are demand and lead time. To overcome thisÂ situation, a simple methodology can be applied by determining an inventory model thatÂ can be used as a basic reference even though probabilistic situation exists. One ofÂ effective methods to make such a prediction is by using simulation. Among the simulationÂ technique that can be applied are Monte Carlo simulation and Exponential Simulation.

CHANGES OF SUCROSE CONTENT AND INVERTASE ACTIVITY DURING SUGARCANE STEM STORAGE Siswoyo, Tri Agus; Oktavianawati, Ika; ., Djenal; Sugiharto, Bambang; Murdiyanto, Untung
Indonesian Journal of Agricultural Science Vol 8, No 2 (2007): October 2007
Publisher : Indonesian Agency for Agricultural Research and Development - MOA

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ABSTRACTInvertases (beta-D-fructofuranosidase, E.C. 3.2.1.26) are the keyenzymes involved in sucrose metabolism in sugarcane plants.They are highly correlated with sucrose and reducing sugar contentsduring plant growth. The sugarcane plants have two kindsof invertases, namely neutral invertase (NI) and acid invertase(AI). They have different function in sucrose accumulation.The research aimed to study the role of AI and NI in accumulationof reducing sugar during storage of sugarcane stems. Plantmaterials of 18-month-old field grown sugarcane of the commercialvariety R-579 (wet-land) and M 442-51 (dry-land) wereused. Three internodes were sampled to represent immature(internode 1-8/F1), maturing (internode 9-16/F2), and mature(internode 17-24/F3) stem tissues. All tissues were stored for0-9 days at room temperature (28-32oC) and each day, the samplewas extracted to determine invertase activity, total solubleprotein, and sugar contents. This observation was valid forinvertase activity expressed on a protein basis. At the initiateharvested (0-3 days), NI had a higher specific activity than AIin the sucrose-accumulating region of the sugarcane stems.Negative significant correlation was found between NI specificactivity and sucrose accumulation (r2 = 0.41, P < 0.05). AIshowed a higher specific activity after 4 days harvested and hadnegative correlation with sucrose accumulation (r2 = 0.40, P <0.05). These results showed that NI could be more responsiblein sucrose hydrolisis than AI at early storage of sugarcane stems.

TRANSFORMASI GEN SOSUT1 PADA TANAMAN TEBU MENGGUNAKAN AGROBACTERIUM TUMEFACIENS STRAIN GV 3101 DAN PANGKAL TUNAS TEBU IN VITRO Edia, Edia F.D; Sugiharto, Bambang; Utarti, Esti
BERKALA SAINSTEK Vol 2, No 1 (2014)
Publisher : My Home

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Gen SoSUT1 merupakan gen pengkode protein sucrose transporter yang memfasilitasi proses transpotasi sukrosa dari jaringan fotosintetik (source) ke jaringan pengguna (sink) pada tanaman tebu. Tujuan penelitian ini adalah mendapatkan tanaman tebu transforman melalui transformasi genetik menggunakan vektor Agrobacterium tumefaciens yang membawa gen SoSUT1. Eksplan pangkal tunas tebu in vitro diinfeksi dengan A. tumefaciens yang membawa konstruk pAct-SoSUT1 dan dilakukan seleksi pada media MS dengan penambahan antibiotik hygromycin.. Tanaman putatif transforman yang telah berhasil melewati proses seleksi dan diaklimatisasi, dilakukan isolasi DNA genom kemudian dianalisis PCR. Hasil analisis PCR dengan pasangan primer 1F/1R hpt II menunjukkan bahwa dari 24 tanaman tebu putatif transforman, didapatkan 15 tanaman tebu positif mengandung gen SoSUT1. Efektifitas rata-rata transformasi gen SoSUT1 menggunakan eksplan pangkal tunas tebu in vitro sebesar 6,8%. Kata Kunci: Agrobacterium tumefaciens, SoSUT1, sukrosa.

Overexpression Sucrose Transporter Protein (Sut) and Sucrose Content In Genetically Modified Product (Gmp) Sugarcane(Saccharum officinarum L.) Dewanti, Parawita; Okviandari, Purnama; Oktaria, Nina; Sugiharto, Bambang
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UNEJ e-Proceeding

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Sucrose transporter (SUT) is a protein that functions to translocate sucrose from source to sink. Protein activity is determined by the accumulation of sucrose in sugarcane stem. The level of sucrose accumulation is also influenced by the activity ofÂ sucrose phosphat synthase (SPS) and neutral invertase (NI) in conjunction with the synthesis and hydrolysis of sucrose. This aim of this study is to determine the sucrose transporter protein expression in Genetically Modified Product (GMP) of sugarcane.Genetically Modified Product GMP sugarcane events 1, 2, 3, 4, 5, 18, 20 and non GMP were used as materials. The analysis was performed by Western Blot method to observe the presence of SUT protein expression, stem sucrose content by resorcinol method to observe the translocation of sucrose, the SPS enzyme activity and NI that play a role in determining the accumulation of sucrose.The results showed that (1) an increase in SUT protein content in GMP plants events 2 and 18 with a protein band that is thicker than the non GMP plants, (2) SPS and invertase activity at all events GMP crop plants to increase compared to non GMP. Sucrose content of the stem at all events GMP plant to increase with age segment. The older age segments, the higher the sucroseÂ content. Keywords: sugarcane (Saccharum officinarum L.), genetically modified product(GMP), sucrose transporter protein(SUT), sucrose

The Future of The Mind: Michio kaku Sugiharto, Bambang
Extension Course Filsafat ( ECF ) 2017: Phylosophy of Mind
Publisher : Extension Course Filsafat ( ECF )

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The Future of The Mind: Michio kaku

Preservation Effect Of Peg (Polyethylene Glycol) In Sugarcane (Saccharum officinarum) Nxi 1,3 Synthetic Seed Ningtiyas, Wulan Nursyiam; Dewanti, Parawita; Sugiharto, Bambang
ANNALES BOGORIENSES Vol 20, No 2 (2016): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Encapsulation is a method for embryogenic tissue coating system by Na-alginate which has synthetic seed preservation purpose. To produce sugarcane synthetic seed need Coleoptillar embryogenic callus and apical bud that it is encapsulated by encapsulation media (MS0 + 4% Na-alginat+ 100 mM CaCl2). In this study, PEG (0%, 1%, 2%, 3%) and PBZ (0 ppm, 1 ppm, 2 ppm, 3 ppm) add to media encapsulation which are give desiccation effect (PEG) and Giberellin synthesis blockade system. Both of PEG or PBZ is used to sugarcane synthetic seed growth retardation that it is implied as storage period. The result are show that PEG 2% is the most effective concentration which is added in encapsulation media. Dessiccation effect of PEG 2% can make synthetic seed storage period until 3 weeks, synthetic seed regeneration produce 89,32 planlets and planlets clorophill concentration is 26,03 ????g/mL. PBZ 1 ppm is the most effective concentration to make storage period until 5 weeks, synthetic seed regeneration produce 39 planlets and planlets clorophill concentration is 35,81 ????g/mL.

KARAKTERISTIK MODUL PENYERAP ENERGI IMPAK MEKANISME INTERNAL INVERSION DAN AXIAL SPLITTING Setiawan, Rachman; Amir, Muhammad Hisyam; Sugiharto, Bambang; Fajrianto, Sigit
Mesin Vol 21, No 2 (2006)
Publisher : Mesin

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The application of crashworthiness technology to a vehicle has a main objective of protecting the passenger or cargo from the effects of impact/collision. One of the strategies is by using impact energy absorbing (IEA) modules. Two of the alternatives of IEA modules are metallic tubes with internal inversion and controlled axial splitting mechanism. This paper presents both numerical and experimental approaches to understand the characteristics of the two mechanisms, before using them in design phase. LS-Dyna was used as the numerical simulation software for drop test case. The result of simulation is presented as the relationships between geometrical parameters and the crashworthiness parameters, e.g. impact energy and response force. Some cases are compared with quasi-static and drop test results.

OPTIMASI TRANSFORMASI GENETIKA MELALUI AGROBACTERIUM PADA TANAMAN PADI Hazmi, Muhammad; Ermawati, Netty; Sugiharto, Bambang
AGRITROP Vol 12, No 1 (2014): Agritrop: Jurnal Ilmu-Ilmu Pertanian
Publisher : Universitas Muhammadiyah Jember

Keberhasilan transformasi genetika pada tanaman padi sangat dipengaruhi oleh karakternya yang bersifat recalsitrant, terutama padi jenis indica yang banyak ditanam oleh petani di Indonesia. Oleh karena itu, optimasi metode selalu diperlukan sebelum melakukan transformasi genetika pada tanaman padi. Transformasi gen GUS sering digunakan sebagai sarana optimasi metode transformasi sebelum melakukan overekspresi gen yang sesungguhnya. Penelitian ini bertujuan untuk memastikan bahwa komponen transformasi gen melalui Agrobacterium tumefaciens dapat bekerja secara efektif pada tanaman padi. Penelitian dilaksanakan di Laboratorium Biosain Politeknik Jember dari bulan Maret sampai dengan Desember 2013. Konstruk gen GUS diperoleh dari Laboratorium Biologi Molekuler Universitas Jember. Padi yang digunakan adalah varietas Inpari 20 dari BPTP Malang dan CV Dongjin dari Korea Selatan sebanyak 20 biji dari setiap verietas. Hasil penelitian menunjukkan bahwa transformasi gen GUS melalui Agrobacterium tumefaciens berhasil menginfeksi eksplan. Eksplan yang diinfeksi tumbuh pada media seleksi antibiotik higromisin sampai umur 65 hari setelah infeksi. Hasil uji GUS menunjukkan bahwa gen GUS tidak terekspresi pada tanaman Padi. Hal ini menunjukkan bahwa ada kemungkinan DNA promoter dan plasmit pCAMBIA tidak kompatibel pada tanaman padi. Agrobacterium tumefaciens dapat digunakan untuk transformasi genetika pada tanaman padi, tetapi perlu DNA promoter dan plasmit yang kompatibel.Kata kunci: Optimasi, transformasi, gen GUS, Agrobacterium tumefaciens, dan padi.

TRANSFORMASI GENETIK DAN EKSPRESI MUTAN SUCROSE PHOSPHATE SYNTHASE PADA TANAMAN TOMAT Fibriani, Suwinda; Agustien, Inyana Dwi; Sawitri, Widhi Dyah; Sugiharto, Bambang
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol 6, No 1 (2019): June 2019
Publisher : Badan Pengkajian dan Penerapan Teknologi (BPPT)

Genetic Transformation and Expression of Sucrose Phosphate Synthase Mutant in Tomato Plant ABSTRACTSucrose phosphate synthase (SPS) is a key enzyme responsible for sucrose biosynthesis. In its regulation, SPS activity is modulated by an allosteric effector glucose-6-phosphate (G6P) suggested to have an ability to bind SPS N-terminus domain. To understand the role of N-terminus in regulating SPS, the SPS gene was mutated with the deletion of N-terminus domain (∆N-SPS). The ∆N-SPS gen was transformed into tomato plants with 5% transformation efficiency. Three transgenic tomato plant 4.20, 5.5.1, and 5.10 were obtained and confirmed by PCR analysis. Transgenic tomato expression was characterized by enzymatic analysis. Result showed that the G6P allosteric regulation in transgenic ∆N-SPS had lost and the SPS activity increased by 2-fold compared to non-transgenic plant. This showed that N-terminus domain-deleted SPS could be actively expressed in plant. Keywords: enzyme, genetic transformation, N-terminus domain deletion, sucrose phosphate synthase, tomato ABSTRAKSucrose phosphate synthase (SPS) merupakan enzim kunci yang bertanggung jawab dalam sintesis sukrosa. Dalam regulasinya, aktifitas SPS dipengaruhi oleh alosterik efektor glukosa-6-fosfat (G6P) yang diduga dapat berikatan pada domain N-terminus SPS. Untuk mengetahui peran N-terminus pada regulasi SPS, dilakukan mutasi SPS dengan penghilangan domain N-terminus (∆N-SPS). Gen ∆N-SPS diinsersi pada tanaman tomat melalui transformasi genetik dengan efisiensi transformasi 5%. Tiga tanaman transgenik tomat (event4.20; 5.5.1; dan 5.10) didapatkan dan positif terkonfirmasi melalui analisis PCR. Ekspresi mutan dikarakterisasi melalui analisis enzimatik. Hasil menunjukkan bahwa tanaman tomat transgenik ∆N-SPS tidak dipengaruhi regulasi alosterik G6P dan aktifitas SPS 2 kali lipat lebih tinggi daripada tanaman bukan transgenik. Ini menunjukkan bahwa SPS dengan delesi domain N-terminus dapat terekspresi aktif pada tanaman. Kata Kunci: delesi domain N-terminus, enzim, sucrose phosphate synthase, tomat, transformasi genetik

Peranan Sucrose Phosphate Synthase (SPS) dan Acid Invertase (AI) Internoda Tebu (Saccharum officinarum L.) dalam Akumulasi Sukrosa -, MISWAR; SUGIHARTO, BAMBANG; HANDOYO, TRI; AYU MADE, SRI
AGRITROP Vol. 26, No. 4 Desember 2007
Publisher : AGRITROP

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Sucrose content is an important component of yield in sugarcane (Saccharum officinarum L.) juice and itssynthesis is determines by the activity of Sucrose phosphate synthase (SPS; EC. 2.4.1.14). This enzyme catalyzes thepenultimate reaction of the formation of sucrose from fructose-6-phosphate (F6P) and uridine-5-diphospho glucose(UDPG). Sucrose is synthesized on leaf and translocated into stalk at where it is accumulated to be accumulatedit. In contrast to SPS, acid invertase (AI) is an enzyme that hydrolyze sucrose to glucose and fructose. The abilityof sugarcane to accumulate the sucrose in stalk is determined by the differences between SPS and AI activities.This study was aimed know role of the internodes SPS and AI in sucrose accumulation in the internodes of somesugarcane varieties. SPS, AI and sucrose were extracted from internodes 1, 3 and 5 of 9 month old sugarcane. Theresults showed that sucrose content for all varieties increased concomitant with the rise in the age of internodes.The decrease in AI activity in internode caused the increase in sucrose content in internodes. Meanwhile, highersucrose content in the older internodes was not followed by higher in SPS activity. Generally, there was strongnegative correlation between sucrose content of internodes with SPS and AI activity. In conclusion, the ability ofsugarcane to accumulate sucrose at internodes was primary determined by AI activity, while SPS activity had asmall role on sucrose accumulation at internodes.

Co-Authors A Bagus Nur Sudrajat A. Dinar Ambarwati Agustien, Inyana Dwi Agustien, Inyana Dwi Amir, Muhammad Hisyam Anjas Wida Elistia Rini Bambang Sugiharto Dedy Eko Rahmanto Dian Al Ghifari Perwitasari Djenal . Edia F.D Edia, Edia F.D Edy Listanto Eny I. Riyanti Erlia Narulita Esti Utarti Fajrianto, Sigit Fibriani, Suwinda Fibriani, Suwinda Husin, Andriati Amir Ika Oktavianawati Imas Cintamulya Intan Ria Neliana Inyana Dwi Agustien Iryani Endah Febrianti Jayus Jayus Kusmana Kusmana Laily Ilman Widuri, Laily Ilman Laksono Trisnantoro Lasino Lasino, Lasino MISWAR - Muhammad Hazmi Mukhamad Su'udi Nadya Oktafiana Nasution, Annio Indah Lestari Netty Ermawati Nina Oktaria, Nina Ningtiyas, Wulan Nursyiam Nurhayati Parawita Dewanti Purnama Okviandari, Purnama Rachman Setiawan Restanto, Didik Pudji Rinda Media Ningtyas Sigit Soepardjono Siti Rohimah Siti Umayyah SRI AYU MADE Sukardiman Suwinda Fibriani Toto Hadiarto Tri Agus Siswoyo TRI HANDOYO Tri J. Santoso Tri Ratnasari Untung Murdiyanto Widhi Dyah Sawitri Wulan Nursyiam Ningtyas Yayuk Fatmawati Yoga Yuniadi Yudi Rinanto Yundari, Yundari

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