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All Journal Biocelebes JURNAL PHOTON Collaborative Medical Journal (CMJ) Nommensen Journal of Medicine Jurnal Abdidas E-Amal: Jurnal Pengabdian Kepada Masyarakat
Deinike Wanita Marwan
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INVESTIGASI ELEMEN PADA SEL SERVIKS NORMAL DENGAN MENGGUNAKAN SPECTRUM DARI MIKROSKOP ELEKTRON Ratih Ayuningtiyas; Deinike Wanita Marwan; Yessi Jusman
Collaborative Medical Journal Vol 1 No 3 (2018): September
Publisher : LPPM Universitas Abdurrab

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Abstract

Jumlah penderita kanker serviks di negara berkembang adalah sangat besar. Berbagai usaha dilakukan untuk mengurangi angka kematian akibat penyakit kanker ini. Penelitian ini bertujuan menginvestigasi elemen pada sel serviks normal dengan menggunakan spektrum dari mikroskop elektron. Metode yang digunakan adalah eksperimen untuk pengujian sel sampel dengan mikroskop elektron. Sampel sel serviks yang digunakan diperoleh dengan bantuan teknisi patologi di Hospital University of Malaya. Sebelum pengujian dengan mikroskop eletron, tentunya persiapan sampel sangat diperlukan. Setelah persiapan sampel dilakukan, sampel yang sudah siap digunakan untuk melihat keberadaan elemen yang terkandung dalam sel serviks dengan menggunakan mikroskop elektron. Analisis statistik digunakan untuk melihat kandungan elemen tersebut pada sel serviks manusia. Hasil dari penelitian kami ini adalah beberapa elemen seperti Karbon, Oksigen, Nitrogen, Natrium, dan lain-lain terdeteksi dengan jelas dengan kandungan persentasi berat yang diperoleh dari software mikroskop elektron. Sehingga, penelitian ini dapat disimpulkan berhasil mendeteksi elemen pada sel serviks manusia dengan jelas.
PENGARUH EKSTRAK ETANOL 96% DAUN KEMANGI (OCIMUM AMERICANUM L) TERHADAP BERAT TESTIS DAN KONSENTRASI SPERMATOZOA TIKUS PUTIH JANTAN (RATTUS NOVERGICUS) May Valzon; Deinike Wanita Marwan
Collaborative Medical Journal Vol 1 No 3 (2018): September
Publisher : LPPM Universitas Abdurrab

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Abstract

Kejadian infertilitas pria merupakan 36% dari seluruh kejadian infertilitas di dunia. Sebanyak 27,8% merupakan infertilitas idiopatik yang salah satu penyebabnya adalah reactive oxygen species (ROS). Ekstrak etanol 96% daun kemangi (Ocimum americanum L.) diduga mampu memperbaiki masalah fertilitas karena aktivitas zat yang dikandungnya, yaitu: flavonoid (orientin dan vicenin), zink, boron, dan arginin. Penelitian ini bertujuan untuk menganalisis pengaruh ekstrak etanol 96% kemangi terhadap berat testis dan konsentrasi spermatozoa tikus putih jantan. Dua puluh empat ekor tikus jantan dibagi secara acak menjadi empat kelompok, masing-masing terdiri dari 6 tikus. Kelompok A, B, C akan diberikan ekstrak kemangi oral setiap hari selama 20 hari dengan dosis 50 mg/kgBB/hari, 100 mg/kgBB/hari, dan 200 mg/kgBB/hari, sedangkan kelompok D adalah kontrol. Semua kelompok diberikan makan dan minum ad libitium selama 20 hari. Tepat pada hari ke-21 dilakukan pengangkatan testis dan pemeriksaan berat testis dan konsentrasi spermatozoa. Data yang diperoleh akan dianalisis secara kuantitatif dengan one way anova. Terdapat perbedaan bermakna rata-rata berat testis di dalam dan antar kelompok, tetapi tidak ditemukan perbedaan signifikan antar kelompok kontrol dan kelompok A dan B. Terdapat perbedaan bermakna rata-rata konsentrasi spermatozoa di dalam dan antar kelompok perlakuan, kecuali pada antara kelompok kontrol dengan kelompok A dan kelompok A dengan kelompok B. Penulis menyimpulkan bahwa pemberian ekstrak etanol 96% kemangi dapat meningkatkan berat testis dan konsentrasi spermatozoa.
AKTIVITAS PEMBERIAN MADU ASAL BASERAH KUANTAN SINGINGI TERHADAP PERTUMBUHAN STAPHYLOCOCCUS AUREUS Eliya Mursyida; Deinike Wanita Marwan
Collaborative Medical Journal Vol 2 No 2 (2019): Mei
Publisher : LPPM Universitas Abdurrab

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Abstract

Madu merupakan cairan kental yang dihasilkan oleh lebah dan memiliki aktivitas sebagai antimikroba. Staphylococcus aureus (S. aureus) adalah bakteri Gram positif penyebab infeksi nosokomial, keracunan makanan, dan sindroma syok toksik. Penelitian ini bertujuan untuk menganalisis aktivitas pemberian madu terhadap pertumbuhan bakteri S. aureus. Metode pada penelitian ini adalah eksperimental dengan post only with control group menggunakan difusi kertas cakram. Hasil penelitian menunjukkan bahwa madu memiliki aktivitas dalam menghambat pertumbuhan bakteri S. aureus dengan zona hambat tertinggi yaitu 5,33mm pada konsentrasi 100% dan terrendah pada konsentrasi 25% dengan zona hambat yaitu 3,00mm.
PENINGKATAN KUALITAS PELAYANAN KESEHATAN DENGAN SOSIALISASI PELAKSANAAN POSYANDU PADA MASA PANDEMI COVID 19 DAN PENERAPAN APLIKASI MOBILE POSYANDU-Q Ramalia Noratama Putri; Debi Setiawan; Deinike Wanita Marwan; Yenny Desnelita; Gustientiedina Gustientiedina; Irwan Irwan
E-Amal: Jurnal Pengabdian Kepada Masyarakat Vol 1 No 3: September 2021
Publisher : LP2M STP Mataram

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Abstract

Untuk meningkatkan Pelayanan Kesehatan pada Keluarahan Sialang Munggu maka posyandu sri mersing memiliki beberapa permasalahan yang dihadapi yaitu : 1) Minimnya minat masyarakat pada RW 18 untuk datang membawa batita dan balita ke posyandu terutatama pada ibu muda. 2) Tidak jalannya Posyandu pada masa pandemic covid-19. 3) Belum tersedianya IPTEK pada posyandu. 4) Posyandu lansia dan pengendalian PTM belum dijalankan sepenuhnya. Berdasarkan permasalahan yang dihadapi mitra maka solusi dari permasalahan tersebut yaitu: 1) sosialiasasi dengan tranformasi digital kesehatan menggunakan smartphone pada ibu muda umur 25-30. 2) pelatihan ke kader posyandu tentang tentang peraturan pelaksanaan kegiatan posyandu pada masa pandemic covid 19. 3) Implementasi aplikasi PosyanduQ Sri Mersing 4) Sosialisasi panduan pelaksanaan posyandu lansia dan cek kesehatan PTM. Metode pelakasanaan kegiatan adalah perancangan, pelatihan, dan implementasi. Setelah kegiatan dilakasanakan maka tahapan evaluasi dilakukan dengan cara menyebarkan kuesioner kepada kader dan masyarakat RW 18..
ISOLATION OF LACTIC ACID BACTERIA FROM TEMPOYAK AND ITS ANTIMICROBIAL ACTIVITY ON THE Escherichia coli Eliya Mursyida; Fifi Candita; Muhammad Faisal; Deinike Wanita Marwan
Biocelebes Vol. 16 No. 1 (2022)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/bioceb.v16i1.15833

Abstract

Diarrhea is a potential extraordinary event that can cause death. The most common microbes that cause diarrhea in developing countries are Rotavirus and Escherichia coli. One of the treatment of diarrhea is by giving antibiotics. However, the use of antibiotics is known to disrupt the balance of normal gastrointestinal flora, thereby changing the composition of the microbiota. Probiotics can be given to balance the normal flora during or after treatment with antibiotics. Lactic acid bacteria (LAB) is one of the probiotics that can inhibit the growth of pathogenic microbes and is commonly found in fermented foods. This study aimed to isolate LAB from tempoyak made from kampar durian, Riau Province, Indonesia and test its antimicrobial activity against Escherichia coli. The study was initiated by isolating LAB from tempoyak using the multilevel dilution method, followed by characterizing LAB by colony and cell morphology, and testing its antimicrobial activity against Escherichia coli using the agar well diffusion method. The antimicrobial activity test results were analyzed using one-way ANOVA and Bonferroni follow-up test. The results of LAB characterization showed that four LAB isolates had different colony morphology, including spherical Gram-positive bacteria and catalase-negative bacteria. The results of the antimicrobial activity test showed that LAB1 isolates had the highest ability to inhibit Escherichia coli with an average inhibition zone diameter of 15.21 mm and the lowest inhibition zone was found in LAB 4 isolates, which was 10.80 mm. The results of the one way ANOVA test showed a significant difference between the four LAB isolates in inhibiting Escherichia coli with P value <0.05. In the Bonferroni test, there were significant differences between LAB 1 and LAB 4 isolates. It can be concluded that LAB isolated from tempoyak has the potential to be a source of probiotics.
Efektivitas Ekstrak Spirulina platensis sebagai Antiinflamasi terhadap Jumlah Neutrofil dan Makrofag pada Luka yang Diinfeksi Staphylococcus aureus pada Tikus Wistar Riski dwi utami; Olvaria Misfa; Bimby Irenesia; Deinike Wanita Marwan
Nommensen Journal of Medicine Vol 8 No 1 (2022): Nommensen Journal of Medicine: Edisi Agustus
Publisher : Universitas HKBP Nommensen

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36655/njm.v8i1.735

Abstract

ABSTRAK Latar belakang: Kandungan senyawa aktif dari Spirulina sp. memiliki aktivitas antiinflamasi dan antibakteri. Penggunaan senyawa tersebut berperan dalam proses inflamasi pada luka yang terinfeksi. Tujuan: Menganalisis efektifitas pemberian ekstrak Spirulina platensis terhadap jumlah neutrofil dan makrofag pada luka insisi tikus wistar yang diinfeksikan S. aureus. Metode: Penelitian ini menggunakan randomized post test only control group design. Dua puluh empat ekor tikus wistar jantan diinsisi kulitnya dan diinfeksikan S.aureus dibagi menjadi 4 kelompok, yaitu kelompok yang diberi ekstrak S.platensis dosis 500 mg/kgBB/hari, dosis 750 mg/kgBB/hari, kelompok kontrol negatif diberi larutan salin serta kelompok kontrol positif dengan pemberian amoksisilin 150 mg/kgBB peroral. Jumlah neutrofil dan makrofag dihitung pada pemeriksaan histopatologis dari jaringan luka pada hari ke-14 yang mencakup 5 lapang pandang. Analisis data dilakukan dengan uji one way ANOVA dan dilanjutkan dengan Post Hoc Test LSD. Hasil: Rerata jumlah neutrofil hari ke-14 pada kelompok dosis 500 mg/kgBB/hari, 750 mg/kgBB/hari, kontrol positif dan kontrol negatif adalah 17,83; 12,17; 5,17; dan 8,83 dengan p < 0,001. Jumlah makrofag hari ke-14 pada kelompok dosis 500 mg/kgBB/hari, 750 mg/kgBB/hari, kontrol positif dan kontrol negatif adalah 7,17; 10,83; 16,83; 15,83 dengan p < 0,002. Uji Post Hoc menemukan jumlah neutrofil pada kelompok dosis 500 mg/kgBB/hari secara signifikan lebih tinggi dibandingkan kelompok lainnya. Sementara, uji Post Hoc untuk jumlah makrofag menemukan perbedaan signifikan hanya pada kelompok dosis 500 mg/kgBB/hari terhadap kelompok kontrol positif dan kontrol negatif. Simpulan: Pemberian ekstrak Spirulina platensis dosis 500mg/kgBB/hari secara signifikan meningkatan jumlah neutrofil dan menurunkan jumlah makrofag pada luka insisi tikus wistar yang diinfeksikan Staphylococcus aureus . Kata kunci: luka, makrofag, neutrofil, Spirulina platensis ABSTRACT Background: The active compound of Spirulina sp. has anti-inflammatory and antibacterial property. The use of these contents plays a role in the inflammatory process in infected wounds. Objective: To analyse the effectiveness of Spirulina platensis extract on the number of neutrophils and macrophages in the incision wound of Wistar rats infected by S. aureus. Methods: This study used a randomized posttest-only control group design. Twenty-four male Wistar rats had their skin incised and infected with S. aureus were divided into 4 groups. The group was given with the extract of S. platensis at a dose of 500 mg/kgBW/day (1), a dose of 750 mg/kgBW/day (2), the negative control group was given saline solution(3), and the positive control group was given amoxicillin 150 mg/kg body weight orally (4). The number of neutrophils and macrophages was counted on histopathological examination of the wound tissue on day 14 which included 5 visual fields. Data analysis was carried out by one way ANOVA test and followed by LSD Post Hoc Test . This study used a randomized posttest-only control group design. Twenty-four male wistar rats were divided into 4 groups, the groups were given S. platensis extract 500 mg/kgBW/day and 750 mg/kgBW/day, the positive control group was given amoxicillin 150 mg/kgBW orally and the negative control group was given saline solution. The skin of the mice was incised and infected with S. aureus. Histopathological examination of wound tissue was performed on day 14 to assess the number of neutrophils and macrophages. Data analysis was carried out with the oneway ANOVA test. Results: The mean numbers of neutrophils on the 14th day in the group of a dose of 500 mg/kgBW/day, 750 mg/kgBW/day, positive control, and negative control were 17.83; 12.17; 5.17; and 8.83 with p < 0.001, respectively. The numbers of macrophages on the 14th day in the group of a dose of 500 mg/kgBW/day, 750 mg/kgBW/day, positive control, and negative control were 7.17; 10.83; 16.83; 15.83 with p < 0.002, respectively. The Post Hoc test exhibited that the neutrophil count in the group of 500 mg/kgBW/day was significantly higher than the other groups. Meanwhile, the Post Hoc test for the number of macrophages found a significant difference, only in the group of a dose of 500 mg/kgBW/day against the positive and negative control groups. Conclusion: There was a significant decrease in the number of macrophages in the group of wistar rats that were incised and infected with Staphylococcus aureus and given Spirulina platensis extract at a dose of 500mg/kgBW/day. Keywords: wound, macrophages, neutrophils, Spirulina platensis
Sosialisasi Pelaksanaan Posyandu Melalui Transformasi Digital Kesehatan Menggunakan Aplikasi Mobile Posyandu Q Ira Puspita Sari; Debi Setiawan; Deinike Wanita Marwan
Jurnal Abdidas Vol. 3 No. 5 (2022): October Pages 785 - 943
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/abdidas.v3i5.691

Abstract

Untuk meningkatkan pelayanan kesehatan pada warga masyarakat di Desa Pulau Gadang XIII Koto Kampar, maka posyandu setempat memiliki beberapa permasalahan yang dihadapi yaitu: 1) Minimnya minat masyarakat sekita untuk dating membawa batita dan balita ke posyandu terutama pada ibu muda. 2) Tidak jalannya Posyandu setelah masa pandemic Covid-19. 3) Belum tersedianya IPTEK pada Posyandu. Berdasarkan permasalahan yang dihadapi mitra maka solusi dari permasalahan tersebut yaitu: 1) Sosialisasi dengan transformasi digital Kesehatan menggunakan smartphone pada ibu muda umur 25-30. 2) Pelatihan ke kader Posyandu tentang peraturan pelaksanaan kegiatan yang ada di Posyandu. 3) Implementasi aplikasi PosyanduQ di Desa Pulau Gadang XIII Koto Kampar. Metode pelaksanaan kegiatan adalah analisis dan perancangan, pendampingan dan pelatihan, implementasi dan pengawasan, serta mentoring dan evaluasi kegiatan. Kegiatan pengabdian ini bertujuan untuk meningkatkan pengetahuan masyarakat melalui transformasi digital dengan memanfaatkan media smartphone.
Antibacterial Activity of Papaya Seeds (Carica papaya L.) Ethanol Extract Against Escherichia coli Eliya Mursyida; Fitria Dina Sella; Deinike Wanita Marwan
Sistem Informasi Vol 13 No 1 (2022): Jurnal Photon
Publisher : LPPM Universitas Muhammadiyah Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37859/jp.v13i1.3699

Abstract

Escherichia coli is a normal flora found in the large intestine of humans and is opportunistic. Acute diarrhea is the discharge of watery or loose stools in larger amounts than normal, lasting less than 14 days. Acute diarrhea is caused by several microbes, one of which is Escherichia coli. Treatment of acute diarrhea caused by bacteria can be given antibiotics, but the relatively high use of antibiotics can lead to resistance. One of the natural ingredients known to treat acute diarrhea is papaya seeds (Carica papaya L.). Papaya seeds are known to have antibacterial activity because they contain flavonoids (quercetin), alkaloids, saponins, and tannins. This study aims to determine and analyze the antibacterial activity of the ethanol extract of papaya seeds on the growth of Escherichia coli. This research is an experimental study with a post-test only with a control group research design using papaya seeds (Carica papaya L.). The test bacteria used in this study were Escherichia coli with the dilution method to determine the Minimum Inhibitory Concentration (MIC) and the spread plate to determine the Minimum Bactericidal Concentration (MBC). The results showed that MIC was present at a concentration of 2%, while MBC was at a concentration of 5%. It was concluded that the ethanol extract of papaya seeds (Carica papaya L.) had antibacterial activity against Escherichia coli.
HUBUNGAN DERAJAT MEROKOK DENGAN KEJADIAN HIPERTENSI PADA PRIA DI DESA X PROVINSI RIAU Wahyu Ramadhan; Nurjamilah Nurjamilah; Ari Diansyah; Deinike Wanita Marwan
Collaborative Medical Journal Vol 5 No 3 (2022): September
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/cmj.v5i3.3281

Abstract

Smoking can cause hypertension due to chemicals contained in tobacco that will cause damage to the inner layer of artery walls and cause the buildup of vascular plaque. According to the World Health Organization (WHO) states that the prevalence of hypertension will increase especially in developing countries. The causes of high prevalence of hypertension are unhealthy lifestyles, smoking habits, stress, lack of exercise, old age, and drinking alcohol. Kotabaru Seberida village is a village whose majority of men are smoking, ranging from those aged less than 20 years to old age. This study aims finding whether there is a relationship between smoking with the incidence of hypertension in men in Kotabaru Seberida Village Keritang District Indragiri Hilir Regency Riau Province. This research use analytic observational method with cross sectional approach. This study was conducted on 52 male samples aged 35-50 years. Blood pressure data were measured with mercury sphygmomanometer while smoking data was measured by questionnaire. Data were analyzed by spearman correlation test. There is a significant relationship between the degree of smoking and the incidence of hypertension in men in Kotabaru Seberida Village, Keritang District, Indragiri Hilir Regency, Riau Province with a p value of 0,000 (p <0.05) and a correlation coefficient (r) of 0.495 with a fairly strong correlation level. Conclusion: There is a significant relationship between the degree of smoking and the incidence of hypertension in men in X village Riau Province
HUBUNGAN AKTIVITAS FISIK DENGAN STATUS GIZI PADA ANAK USIA DIBAWAH 6 TAHUN DI TK PERTIWI KOTA BANGKINANG Deinike Wanita Marwan; Nurlaila Nurlaila; Evy Eryta; Elvina Zuhir; Risnandar Risnandar
Collaborative Medical Journal Vol 6 No 1 (2023): Januari
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/cmj.v6i1.3287

Abstract

Obesity can occur due to an imbalance between energy intake and energy expenditure. Factors that play a role in determining the nutritional status of children are diet and physical activity. The general objective of this study was to determine the relationship between the level of physical activity and nutritional status as assessed based on body mass index in children under 6 years old at Pertiwi Kindergarten, Bangkinang City. This research is a quantitative research that is observational descriptive analytic with a cross sectional approach. The sampling method uses total sampling technique. Data were collected using questionnaires, scales and microtoise to measure body weight and height. This study found that subjects with a normal body mass index slept an average of 11.3 hours, did sedentary activities for 10.90 hours and were active for 1.96 hours a day, while subjects with a body mass index above the average sleeping normally for 13.69 hours, doing sedentary activities for 8.58 hours and doing active activities for 1.13 hours a day. In this study, 60 respondents had a normal body mass index, and 10 respondents had a body mass index who were overweight or obese. There is a significant relationship between physical levels. activity and nutritional status in children under 6 years of age.
Co-Authors Anak Agung Istri Sri Wiadnyani Ari Diansyah Bimby Irenesia Debi Setiawan Debi Setiawan Desnelita, Yenny Eliya Mursyida Eliya Mursyida Elly Anggreny Elvina Zuhir Evy Eryta Fifi Candita Fitria Dina Sella Gustientiedina Gustientiedina Ira Puspita Sari Irwan Irwan Jusman, Yessi Muhammad Faisal Nurjamilah Nurjamilah Nurlaila Nurlaila Olvaria Misfa Ramalia Noratama Putri Ratih Ayuningtiyas Riski dwi utami Valzon, May Wahyu Ramadhan