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. Sugiyanto
Lampung University
Biochemistry, Genetics & Molecular Biology Health Professions Medicine & Pharmacology

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Selectivity of Ethyl Acetate Fraction of Gynura Procumbens on Colon Cancer and Breast Cancer Nurulita, Nunuk Aries; Meiyanto, Edy; Sugiyanto, .
Indonesian Journal of Cancer Chemoprevention Vol 2, No 3 (2011)
Publisher : Indonesian Research Gateway

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Abstract

Gynura  procumbens  is  widely  used  as  traditional  remedy  in  South-East  Asia.  Gynura procumbens exhibites anti inflammatory, antioxidant, and reduced blood pressure activity. The aim of this study was to determine chromatographic profile of ethyl acetate fraction of  Gynura procumbens (FEG) and to investigate its cytotoxic properties and selectivity to colon cancerand breast cancer cancer cells. The chromatographic profile of FEG was determined using HPTLC densitometric  and  HPLC.  MTT  (3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium  bromide) assay was performed to determine the growth inhibitory effect of FEG on the growth of WiDr, MCF-7, and T47D cells. NIH3T3, a normal cells was used to determine the selectivity of FEG, which  contained  small  amount  of  quercetin  as  identified  from  chromatographic  profile  both HPTLC  and  HPLC.  FEG  inhibited  cell  growth  of  WiDr,  of  MCF-7  and  of  T47D  cells  in  time dependent manner. Quercetin affected cell growth inhibition approximately two fold higher at WiDr and MCF-7, whereas FEG had lower effect on T47D cell. Quercetin did not seem as the main  active  compound  of  FEG.  At  this  study,  FEG  caused  less  inhibition  on  the  growth  of NIH3T3 cells than that of on all cell lines. Selectivity index (SI) of FEG on WiDr, MCF-7 and T47D were 4.97, 2.77 and 7.79 respectively. According to the datas obtained, FEG possesses moderate to high cytotoxicity properties on WiDr, MCF-7 and T47D cells. FEG demonstrates selective  effect  against  cancer  cells  and  reveals  prospective  properties  as  cancer chemoprevention agent.Keywords: Gynura procumbens, colon cancer, breast cancer, cytotoxicity, selectivity
Validasi Metode Penetapan Kadar Lisinopril dalam Spiked Plasma Secara Ultra Performance Liquid Chromatography Melalui Derivatisasi dengan 1-Fluoro 2,4 Dinitrobenzen Sumiyani, Ririn; Martono, Sudibyo; Sugiyanto, .
JFIOnline | Print ISSN 1412-1107 | e-ISSN 2355-696X Vol 8, No 1 (2016)
Publisher : Indonesian Research Gateway

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ABSTRACT: A rapid, accurate, and sensitive method for determining lisinopril in spiked plasma was developed by means using an Ultra Performance Liquid Chromatography (UPLC) with 1-fluoro 2,4 dinitrobenzen (FDNB) derivatization. Lisinopril was precolumn derivatized with FDNB at optimum condition, i.e. room temperature and borate buffer at pH 11, subsequently analyzed with UPLC. Isocratic condition of acetate buffer (0.01 M, pH 3.50) : acetonitrile : metanol = 70 : 10 : 20 (v/v/v) as mobile phase, 0.3 mL/min of flow rate at λ 296 nm were applied at Acquity BEH C18 column, resulting a linearity of lisinopril at range of concentration of 5,0-100 ng/mL (Y = 410,59x + 211,91, r = 0.93). The accuration of the established method was achieved by 88,59±6,01 to 101,70± 2,56% recovery, while the precision was shown with RSD value of 2,57- 8,16 %, limit of detection (LOD) instrument of 0,73 ng/mL and limit of quatification (LOQ) 2,44 ng/mL, dwith R2 = 0,9987 dan r = 0,9993. In addition, the resulted LOD and LOQ more or less similar with the published HPLC-MS-MS method (1.03-10.0 ng/mL). Hence, it could be concluded that the developed UPLC method can be used as an alternative method for determining lisinopril in plasma.Keywords: Lisinopril, FDNB, derivatization, UPLCABSTRAK: Penetapan kadar lisinopril dalam spiked plasma secara Ultra Performance Liquid Chromatography (UPLC) melalui derivatisasi dengan 1-fluoro 2,4 dinitrobenzen (FDNB) merupakan metode yang cepat, sensitif dan akurat. Derivatisasi precolumn lisinopril dan FDNB optimum pada suhu kamar, suasana dapar borat pH 11,0, dilanjutkan analisis secara UPLC isokratis menggunakan kolom Acquity BEH C dan fase gerak dapar asetat (0,01 M pH 3,50): asetonitril:metanol (70: 10: 20, v/v/v), laju alir 0,3 mL/menit pada λ 296 nm, menghasilkan linieritas kadar lisinopril dalam spiked plasma pada rentang 5,0 -100 ng/mL terhadap luas area lisinopril-DNB dengan persamaan Y = 410,59x + 211,91 dengan R2 = 0,9987 dan r = 0,9993 Akurasi metode ditunjukkan dengan nilai % rekoveri sebesar 88,59±6,01 smpai dengan 101,70± 2,56 %. Ketelitian ditunjukkan dengan nilai RSD 2,57- 8,16 %, sedangkan Batas Deteksi Instrumen = 0,73 ng/ mL dan Batas Kuantitasi = 2,44 ng/mL. Hasil Batas Deteksi penelitian ini relatif sama dengan Batas Deteksi penetapan kadar lisinopril secara HPLC-MS (1,03- 10,0 ng/mL). Dengan demikian dapat disimpulkan bahwa metode ini berpotensi dikembangkan sebagai metode alternatif pengganti HPLC-MS untuk penetapan lisinopril dalam plasma.Kata kunci: Lisinopril, FDNB, derivatisasi, UPLC
Validasi Metode Penetapan Kadar Lisinopril dalam Spiked Plasma Secara Ultra Performance Liquid Chromatography Melalui Derivatisasi dengan 1-Fluoro 2,4 Dinitrobenzen Sumiyani, Ririn; Martono, Sudibyo; Sugiyanto, .
Jurnal Farmasi Indonesia Vol 8, No 1 (2016)
Publisher : Jurnal Farmasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (418.293 KB) | DOI: 10.35617/jfi.v8i1.417

Abstract

ABSTRACT: A rapid, accurate, and sensitive method for determining lisinopril in spiked plasma was developed by means using an Ultra Performance Liquid Chromatography (UPLC) with 1-fluoro 2,4 dinitrobenzen (FDNB) derivatization. Lisinopril was precolumn derivatized with FDNB at optimum condition, i.e. room temperature and borate buffer at pH 11, subsequently analyzed with UPLC. Isocratic condition of acetate buffer (0.01 M, pH 3.50) : acetonitrile : metanol = 70 : 10 : 20 (v/v/v) as mobile phase, 0.3 mL/min of flow rate at λ 296 nm were applied at Acquity BEH C18 column, resulting a linearity of lisinopril at range of concentration of 5,0-100 ng/mL (Y = 410,59x + 211,91, r = 0.93). The accuration of the established method was achieved by 88,59±6,01 to 101,70± 2,56% recovery, while the precision was shown with RSD value of 2,57- 8,16 %, limit of detection (LOD) instrument of 0,73 ng/mL and limit of quatification (LOQ) 2,44 ng/mL, dwith R2 = 0,9987 dan r = 0,9993. In addition, the resulted LOD and LOQ more or less similar with the published HPLC-MS-MS method (1.03-10.0 ng/mL). Hence, it could be concluded that the developed UPLC method can be used as an alternative method for determining lisinopril in plasma.Keywords: Lisinopril, FDNB, derivatization, UPLCABSTRAK: Penetapan kadar lisinopril dalam spiked plasma secara Ultra Performance Liquid Chromatography (UPLC) melalui derivatisasi dengan 1-fluoro 2,4 dinitrobenzen (FDNB) merupakan metode yang cepat, sensitif dan akurat. Derivatisasi precolumn lisinopril dan FDNB optimum pada suhu kamar, suasana dapar borat pH 11,0, dilanjutkan analisis secara UPLC isokratis menggunakan kolom Acquity BEH C dan fase gerak dapar asetat (0,01 M pH 3,50): asetonitril:metanol (70: 10: 20, v/v/v), laju alir 0,3 mL/menit pada λ 296 nm, menghasilkan linieritas kadar lisinopril dalam spiked plasma pada rentang 5,0 -100 ng/mL terhadap luas area lisinopril-DNB dengan persamaan Y = 410,59x + 211,91 dengan R2 = 0,9987 dan r = 0,9993 Akurasi metode ditunjukkan dengan nilai % rekoveri sebesar 88,59±6,01 smpai dengan 101,70± 2,56 %. Ketelitian ditunjukkan dengan nilai RSD 2,57- 8,16 %, sedangkan Batas Deteksi Instrumen = 0,73 ng/ mL dan Batas Kuantitasi = 2,44 ng/mL. Hasil Batas Deteksi penelitian ini relatif sama dengan Batas Deteksi penetapan kadar lisinopril secara HPLC-MS (1,03- 10,0 ng/mL). Dengan demikian dapat disimpulkan bahwa metode ini berpotensi dikembangkan sebagai metode alternatif pengganti HPLC-MS untuk penetapan lisinopril dalam plasma.Kata kunci: Lisinopril, FDNB, derivatisasi, UPLC
Co-Authors Edy Meiyanto Nunuk Aries Nurulita Ririn Sumiyani Sudibyo Martono