T Suhargiyantatmo
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Feeding Aspergillus oryzae Fermentation Culture (AOFC) to Growing Sheep: 1. The Effect of AOFC on Rumen Fermentation Lubis, Darwinsyah; Wina, Elizabeth; Haryanto, Budi; Suhargiyantatmo, T
Indonesian Journal of Animal and Veterinary Sciences Vol 7, No 3 (2002)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (176.335 KB) | DOI: 10.14334/jitv.v7i3.289

Abstract

Cultures of fungi, especially Aspergillus oryzae, have been of interest to animal nutritionists to increase feed efficiency. Many experiments have been done and showed positive results on rumen fermentation and productivity of ruminants. This paper reports the results of an in vivo study on feeding Aspergillus oryzae, fermentation culture (AOFC) to growing sheep. ‘Onggok’ (tapioca processing waste) was used as media for AO cultivation after being enriched with a mineral mixture. Commercial concentrate (GT-03) was fed to 15 growing sheep supplemented with 0% (C0), 5% (C1), and 10% (C2) AOFC (w/w). Chopped fresh King grass was used as a basal diet. The 3 treatments were randomly allotted to the sheep according to randomized block design with 5 replications. The study was carried out for 14 weeks. Digestion trial was conducted in the last 10 days of experiment. All feed and fecal samples were analyzed for nutrients. Rumen fluid was sampled at the mid experimental period. Analyses were done on rumen pH, ammonia content, (VFA) volatile fatly acids concentration, and also total digestive tract digestibility of dry and organic matter, crude protein, and total fiber (NDF). Differences in treatment means were analyzed by Duncan’s MRT. Feeding AOFC resulted in increased (P<0.05) digestibility of crude protein from 59.6% in control sheep to 65.5% in sheep fed concentrate with 10% AOFC supplementation. The same pattern also occurred for NDF, but no effect was found on dry and organic matter. Higher fiber digestibility with AOFC supplementation was in line with an increase (P<0.05) in cellulolytic bacteria population in the rumen. VFA produced also increased (P<0.05), as well as individual acids content, primarily acetate and propionate. No differences (P<0.05) were detected in rumen pH and ammonia content. It appears that AOFC is more suitable for the purpose of meat production.   Key words: A. oryzae, sheep, digestibility, rumen, in vivo
Feeding of Aspergillus oryzae fermentation culture (AOFC) to growing sheep: 2. Growth rate and feed efficiency D Lubis; B Haryanto; E Wina; T Suhargiyantatmo
Jurnal Ilmu Ternak dan Veteriner Vol 7, No 4 (2002): DECEMBER 2002
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (157.362 KB) | DOI: 10.14334/jitv.v7i4.297

Abstract

Utilization of yeast and or filamentous fungi as feed additive to ruminants has been of interest since the late 1980’s. Two fungi species have been commercially produced in the United States, (1) Yea-Sacc containing living cells of Saccharomyces cerevisiae and (2) Amaferm bearing Aspergillus oryzae fermentation extract. It has been demonstrated and proven that the cultures can enhance rumen development and function in young ruminants. This paper concerns the use of Aspergillus oryzae fermentation culture (AOFC) as feed additive for young-growing male ‘Garut’ sheep. The A. oryzae was cultured in a media made of mineral-enriched ‘onggok’ flour, a material of tapioca processing waste. The AOFC was prepared gradually by incubating the fungus at room temperature (26 – 300C) for 5 days, dried at 400C and ground. The AOFC was added to a commercial concentrate (GT-03) at 0, 5 and 10% (w/w) levels, as treatment C0 (control), C1, and C2, respectively. Fifteen growing ‘Garut’ sheep were used and the concentrate feed treatments were randomly allotted based on a randomized block design. Drinking water was available at all time. The amount of feed offered (chopped King grass and concentrates) and their refusals were weighed daily and live-weight of sheep was measured once a week in the morning. Daily feces was collected and weighed in the last 10 days of the 14-week experimental period. All feed and fecal samples were analyzed for dry matter, crude protein, total fiber (NDF), and ash. AOFC supplementation resulted in higher weight gains (P<0.05), which were 94.81; 122.08; and 140.52 g/d for C0, C1, and C2 treatments, respectively. Dry and organic matter, as well as protein intake was also significantly increased by inclusion of AOFC into concentrate diet (P<0.05). The increment in nutrient intake was from increased consumption of concentrates, and not from King grass, however, there was no effect of AOFC supplementation on feed efficiency. Key words: A. oryzae, sheep, gain, efficiency
Feeding Aspergillus oryzae Fermentation Culture (AOFC) to Growing Sheep: 1. The Effect of AOFC on Rumen Fermentation Darwinsyah Lubis; Elizabeth Wina; Budi Haryanto; T Suhargiyantatmo
Jurnal Ilmu Ternak dan Veteriner Vol 7, No 3 (2002): SEPTEMBER 2002
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (176.335 KB) | DOI: 10.14334/jitv.v7i3.289

Abstract

Cultures of fungi, especially Aspergillus oryzae, have been of interest to animal nutritionists to increase feed efficiency. Many experiments have been done and showed positive results on rumen fermentation and productivity of ruminants. This paper reports the results of an in vivo study on feeding Aspergillus oryzae, fermentation culture (AOFC) to growing sheep. ‘Onggok’ (tapioca processing waste) was used as media for AO cultivation after being enriched with a mineral mixture. Commercial concentrate (GT-03) was fed to 15 growing sheep supplemented with 0% (C0), 5% (C1), and 10% (C2) AOFC (w/w). Chopped fresh King grass was used as a basal diet. The 3 treatments were randomly allotted to the sheep according to randomized block design with 5 replications. The study was carried out for 14 weeks. Digestion trial was conducted in the last 10 days of experiment. All feed and fecal samples were analyzed for nutrients. Rumen fluid was sampled at the mid experimental period. Analyses were done on rumen pH, ammonia content, (VFA) volatile fatly acids concentration, and also total digestive tract digestibility of dry and organic matter, crude protein, and total fiber (NDF). Differences in treatment means were analyzed by Duncan’s MRT. Feeding AOFC resulted in increased (P<0.05) digestibility of crude protein from 59.6% in control sheep to 65.5% in sheep fed concentrate with 10% AOFC supplementation. The same pattern also occurred for NDF, but no effect was found on dry and organic matter. Higher fiber digestibility with AOFC supplementation was in line with an increase (P<0.05) in cellulolytic bacteria population in the rumen. VFA produced also increased (P<0.05), as well as individual acids content, primarily acetate and propionate. No differences (P<0.05) were detected in rumen pH and ammonia content. It appears that AOFC is more suitable for the purpose of meat production.   Key words: A. oryzae, sheep, digestibility, rumen, in vivo