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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy)
Inez H.S. Loeddin Suharsono
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Agriculture, Biological Sciences & Forestry Economics, Econometrics & Finance

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ISOLASI DAN REGENERASI PROTOPLAS DARI MESOFIL DAUN KENT ANG (Solanum tuberosum L) DIHAPLOID Agus Purwito; G. A. Wattimena; Antonius Suwanto; Inez H.S. Loeddin Suharsono; Hajrial Aswidinnoor
Indonesian Journal of Agronomy Vol. 27 No. 1 (1999): Buletin Agronomi
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (925.414 KB) | DOI: 10.24831/jai.v27i1.1579

Abstract

The isolation and regeneration of potato (Solanum tuberosum L) protoplasts have been carried out. Mesophyl cell protoplast were isolated from two dihaploid cultivars of potato BF 15 and SVP 10 leaves used four different enzymes solution. Protoplast were cultured onto four different cultures media to increase plating efficiency. Calli were then transferred to ten different regeneration media. Using cellulose RS 0.5 % and pectolyase Y-23 0.05 % protoplast yield of both cultivars were improved. Medium VKM  supplemented with 0.2 mg/l 2, 4-D, 1.0 mg/l NAA and 0.5 mg/l zeatin or 2iP were increase recovery of colonies from protoplast up to 5.9%. Regeneration medium containing zeatin did always produce more shoots than those of 2iP. Genotypes dependant regeneration frequencies have also been showed in this experiments
Co-Authors Agus Purwito Antonius Suwanto dan Meity S. Sinaga . Budi Tjahjono Andi Khaeruni R G. A. Wattimena HAJRIAL ASWIDINNOOR