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All Journal Jurnal Ilmu Ternak dan Veteriner Journal of the Indonesian Tropical Animal Agriculture
S. Suharti
Department of Nutrition and Feed Technology, Faculty of Animal Science, Bogor Agricultural University
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences Veterinary

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Nutrient digestibility and beef cattle performance fed by lerak (Sapindus rarak) meal in concentrate ration Suharti, S.; Astuti, D.A.; Wina, E.
Indonesian Journal of Animal and Veterinary Sciences Vol 14, No 3 (2009)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (150.538 KB) | DOI: 10.14334/jitv.v14i3.341

Abstract

This research was aimed to study the use of Lerak fruit meal to improve performance and feed digestibility of beef cattle. The research consisted of two trials (in vitro and in vivo studies). The in vitro trial was screening of bioactive compounds (saponin, tanin, dan diosgenin) in Lerak fruit (including seed) and continued to evaluate the effectivity of these compounds against ruminal protozoa. The in vivo study was done using 12 Ongole Crossbreed cattle which received 1of 3 different treatments: 1) concentrate without Lerak as control, 2) concentrate containing 2.5% Lerak, and 3) concentrate containing 5% Lerak. Anti protozoal activity, daily gain, and nutrient digestibility of beef cattle were measured. Results showed that saponin concentration in Lerak extracted by methanol was higher than that in Lerak extracted by water and Lerak meal, 81.5%; 8.2% and 3.85% respectively. Lerak extracted by methanol have higher antiprotozoal activity in vitro than Lerak extracted by water. In vivo experiment showed that there were no significant differences (P>0.05) of nutrient intake and digestibility in all treatments, that means the ration had good palatability and quality. Average daily gain of PO fed 2.5% Lerak was 20% higher than that of control diet (0.9 kg/day). Key words: Sapindus rarak, Protozoa, Performance, Ongole Crossbreed, Digestibility
Survival of Lactobacillus plantarumU40 on the in vitro rumen fermentation quantified with real-time PCR Astuti, W.D.; Widyastuti, Y.; Wina, E.; Suharti, S.; Ridwan, R.; Wiryawan, K.G.
Journal of the Indonesian Tropical Animal Agriculture Vol 43, No 2 (2018): June
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.43.2.184-192

Abstract

The objective of this study was to evaluate the survival of L. plantarumU40 quantified with real-time PCR during in vitro rumen fermentation. The experiment was arranged in a randomized block design with 3 treatments and 4 replications. Treatments were control, rumen fermentation inoculated with L. plantarumU40and L. plantarumU40 + glucose solution. Population of L. plantarum U40 was higher at inoculation treatment. After 8 hours incubation, glucose addition tended to decrease L. plantarum U40 population. Control treatment showed lowest population of L. plantarum U40 along in vitro fermentation compared with other treatment. Inoculation of L. plantarumU40 significantly (p<0.05) increased population of LAB until 12 hours incubation compared with control. Control treatment had highest pH at all incubation time. Glucose addition significantly (P<0.05) decreased final rumen pH (24 hours) (6.30), compared with control treatment (6.85). Inoculation of L. plantarum U40 with glucose addition significantly (P<0.05)increased propionic acid, decreased acetic acid and A/P ratio compared with other treatments. Lactobacillus plantarum U40 without glucose addition did not affect propionic acid production significantly. As conclusion, Lactobacillus plantarum U40 can survive in rumen fluid and changes rumen fermentation when glucose is added as carbon source. 
The expression of heat shock protein 70 gene with organic selenium supplementation and its effetc on productivity of broilers in tropical environment Amizar, R.; Suharti, S.; Jakaria, J.; Mutia, R.
Journal of the Indonesian Tropical Animal Agriculture Vol 42, No 4 (2017): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.42.4.279-287

Abstract

The purpose of this experiment is to study the effect of organic selenium (Se) supplementation on the expression of heat shock protein 70 gene (HSP70), glutathione peroxidase (GSH-Px) enzyme activity, malondialdehyde (MDA) and productivity of broilers in tropical environment. Three kinds of environmental pens were designed in this experiment: comfortable environment pens with temperature of air conditioner adjusted at 22oC (R0), tropical environment pens (±30oC ) without organic Se (R1), and tropical environment pens supplemented with 0.30 ppm organic Se (R2). One hundred and twenty broiler chickens (unisex) were used in this study. There were 40 chicks per pen for each treatment. The experimental design was completely randomized with four replications for each treatment. The data were statistically analyzed using the general linear model of SAS program. Results showed that R0 and R2 groups had significantly increased (P<0.05) feed intake, body weight, body weight gain, and decreased feed conversion ratio compared to R1 groups. Meanwhile, the expression of HSP70, GSH-Px enzyme activity and MDA of R2 groups and R0 groups were significantly lower (P<0.05) than that of R1 groups. It was concluded that the broilers given 0.30 ppm organic Se in tropical environment had similar productivity and expression of HSP 70 with broilers kept in comfortable environment.
Co-Authors Astuti, W.D. D.A. Astuti E. Wina Jakaria Jakaria Luh Putu Ratna Sundari R. Mutia R. Ridwan Robi Amizar Y. Widyastuti