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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Journal of Tropical Horticulture JURNAL AGRONIDA Jurnal Agrium Global Science Society

Rd. Selvy Handayani

Department Of Agroecotechnology, Faculty Of Agriculture, Universitas Malikussaleh, Aceh Utara

Author-ID : 3896768

Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Economics, Econometrics & Finance Education Energy Engineering Environmental Science Public Health Social Sciences

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Pengaruh Batang Bawah dan Jenis Tunas pada Mikrografting Manggis (Garcinia mangostana) secara In Vitro Rd. Selvy Handayani; Roedhy Poerwanto; , Sobir; Agus Purwito; Tri Muji Ermayanti
Indonesian Journal of Agronomy Vol. 41 No. 1 (2013): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

The aim of this study was to investigate the effect of rootstock and shoot types on in vitro mangosteen micrografting.The experiment was arranged in completely randomized design (CRD) with two factors. The first factor was the rootstocktype, i.e. rooted planlet from the germination of quartered seed, and rooted planlet from the germination of undivided seeds.The second factor was the developmental phase of scion, i.e. dormant buds, and flush (had new leaf more than 2-4 mm). Theresults showed that rootstock derived from the germination of undivided seed had a higher success rate than other treatmentson all variables, except for number of new leaves. The use of flush as scion was better than dormant buds; flush resulted in ahigher percentage of successful micrograft and longer shoots. In vitro micrografting had a better growth rate than grafting at the same age. The results of anatomical observation conducted at four months after micrografting demonstrated that there was a good graft union, indicated by excellent fusion between rootstock and scion xylem tissues.Keywords: flush, in vitro, micrografting, rootstock, scion

The Effect of Different Seed Cutting Treatments and Concentrations of BAP for the Successful In Vitro Micrografting of Mangosteen (Garcinia mangostana L.) Mira Agustina; Maisura Maisura; Rd. Selvy Handayani
Journal of Tropical Horticulture Vol 3, No 1 (2020): April 2020
Publisher : Indonesian Society for Horticulture (Perhimpunan Hortikultura Indonesia Komisariat Aceh)

The efforts of the rooting of regenerants resulting from gamma-ray irradiation require plant tissue culture, which known as micrografting. This technique can help irradiated regenerants to develop a well root system, by combining non-rooting shoots with rooted in vitro cultured shoots of plant rootstock. The purpose of this study was to examine the effect of seed explants cutting and the application of different BAP concentrations for the successful micro-grafting of mangosteen in vitro. This experiment employed Complete Randomized Design (CRD) Factorial with two factors and ten replications. The first factor was the cutting treatments of mangosteen seeds explants for rootstock shoots, consisting of 2 types of seeds: uncut and cut seeds. The second factor was BAP concentrations: BAP 0 mg/l and BAP 2 mg/l. The results showed that the division of the seeds had an influence on the results of micro-grafting mangosteen in vitro. Micrografted mangosteen, which rootstock applied from undivided seeds, possessed faster growth, longer shoots, and produce more leaves compared to rootstock shoots from the divided seeds. BAP concentrations also contributed to the results. The application of BAP 2 mg/ demonstrated better effect on all variables observed. There were no interactions between seed divisions and BAP concentrations in all observed variables.

Potential Changes in Watermelon (Citrullus lannatus) Ploidy Treated By Colchicine Rd. Selvy Handayani; Muhamad Yusuf; Ajmir Akmal
Journal of Tropical Horticulture Vol 1, No 1 (2018): October 2018
Publisher : Indonesian Society for Horticulture (Perhimpunan Hortikultura Indonesia Komisariat Aceh)

The purpose of this study was to determine the effect of colchicine on changes in ploidy watermelon. The research was conducted in Agroecotechnology laboratory Universitas Malikussaleh, Microtechnique laboratory Agronomy and Horticulture Departement, Bogor Agricultural University, and Beuringen, Murah Mulia subdistrict, North Aceh. This research used Completely Randomized Design (CRD) two factors. The first factor was watermelon seed soaking time in colchicines 0,02% ie.0, 24,36, and 48 hours. The second factor was the concentration of the colchicine solution on the sprout growth point i.e. 0, 0,1, and 0,2 %. The results showed that plants were given colchicine became to shorter and fewer number of leaves than plants without any treatment. Colchicine could increase the size of the stomata, but it did not change the shape of stomata. Plants that were given colchicine had the potential to ploidy multiplication.

In-vitro Callus Induction of Durian (Durio zibethinus Murr.) Leaves Using Kinetin and 2,4-D (Dichlorophenoxyacetic acid) Rd. Selvy Handayani; Ismadi Yunus; M. Sayuti; Endri Irawan
Journal of Tropical Horticulture Vol 2, No 2 (2019): October 2019
Publisher : Indonesian Society for Horticulture (Perhimpunan Hortikultura Indonesia Komisariat Aceh)

Durian (Durio zibethinus Murr.) is a tropical fruit grown in Southeast Asia and it has high nutritional and economic values and highly appreciated by consumers in Indonesia. Unfortunately, there are several obstacles in planting durian in Indonesia, specifically in Aceh. One of the abstacles is the scarcity of superior durian plants. Generally, plant conservations done conventionally through vegetative propagation. However, this technique has disadvantage such as harming the initial plants. Therefore, plant culture tissue has been used as a modern technique to develop durian plants. The objective of this research was to evaluate the effect of kinetin and 2,4 D on development of durian leaf explants through in-vitro bioassay. The research was conducted at Tissue Culture Laboratory, Faculty of Agriculture, Malikussaleh University from January to April 2019 using Completely Randomized Design (CRD) Factorial with 2 factors observed. The first factor was Kinetin (K): 0.0, 0.1 and 0.5 ppm. The second factor was auxin 2,4 D: 0.0, 0.5 and 1.0 ppm. The results showed that the in-vitro application of kinetin affected the induction of leaf callus. The application of 0.5 ppm of kinetin gave the best result compared to others. The in-vitro application of 2,4 D 0.5 ppm also possessed the best result compared to others. There was no interaction beween in-vitro application of kinetin and 2,4 D on the growth of durian leaf callus at all parameters observed.

The Effect of BAP and IBA on In Vitro Root Cultures of Acehnese Pomelo (Citrus maxima (Burm.) Merr.) Ira Handayani; Laila Nazirah; Rd. Selvy Handayani
Journal of Tropical Horticulture Vol 3, No 1 (2020): April 2020
Publisher : Indonesian Society for Horticulture (Perhimpunan Hortikultura Indonesia Komisariat Aceh)

Acehnese pomelo is considered as a potential fruit to be developed for its economic and nutritional values. However, there is a main problem in developing this fruit. Pomelo sometimes is grown from seeds, but nowadays the fruits are often found to be having lesser seeds or even seedless. Besides, it is quite challenging to grow this local pomelo not only due to the scarce of the seeds, but it also cannot be conventionally germinated. Therefore, we need to develop this fruit through tissue culture. The purpose of this study was to investigate the effect of growth regulators on germination of pomelo seeds in vitro and its organ formation from in vitro root explants seed-originated of pomelo. The study was conducted at Plant Tissue Culture Laboratory, Faculty of Agriculture, Malikussaleh University. In October 2018 to April 2019. The research was divided into two separate experiments: 1) in vitro germination of pomelo seeds and 2) in vitro organ formation from root explants from of pomelo seeds. The first experiment using Complete Randomized Design (CRD) with 10 replications. The only factor observed was the use of BAP (0 and 2 mg/l). The second experiment was the examination of plant organ formation from root explants of pomelo seeds. This experiment had 2 factors observed. The first factor was different concentrations of BAP (0, 1, and 2 mg/l). The second factor was the different concentration of IBA (0, 1.25, and 2.5 mg/l). The results indicated that the application of BAP influenced the germination of pomelo seeds. The application of 2 mg/l BAP attributed to slower growth of shoots and roots, but it demonstrated better number of shoots, roots and leaves compared to application of 0 mg/l BAP. Pamelo root explants can form adventitious shoots on media eventhough without growth regulator (BAP 0 mg/l + IBA 0 mg/l), but optimization still needs to be done in order to obtain the best concentration to initiate shoot formation.

Effect of Cytokines On The In Vitro of Sweet Kaffir Lime (Citrus hystrix Dc) Rd. Selvy Handayani; Ismadi Yunus; Nadia Tillah; Ira Handayani
Journal of Tropical Horticulture Vol 3, No 2 (2020): October 2020
Publisher : Indonesian Society for Horticulture (Perhimpunan Hortikultura Indonesia Komisariat Aceh)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33089/jthort.v3i2.51

Sweet kaffir lime (Citrus hystrix Dc), also called "Boh Kruet Mameh" is one of Aceh's local plants, which is currently very difficult to obtain. Sweet kaffir lime is not like the ordinary kaffir lime, which tastes sour. The fruit of sweet kaffir lime has a larger size than the regular kaffir lime, sweet and sour taste, aromatic and fresh, which can be consumed as a fruit table. The problem of developing the propagation technique of sweet kaffir lime plants is the seeds' condition, which are conventionally difficult to germinate and limited in number, making it difficult to get a high-quality seed. Thus, to overcome these problems, suitable solutions to plant propagation techniques should be sought. As for the right plant propagation technique is tissue culture technology or in vitro propagation technique. This research was conducted at the Plant Tissue Culture Laboratory, Faculty of Agriculture, Malikussaleh University. The research was conducted from February to April 2018. This research used a single randomized complete design (CRD) with ten replications. The treatment is a combination of the basic media, which consists of MS0 (MS media without BAP) and MS1 (MS + BAP 4 ppm). The result showed that the adduction of 4 ppm BAP in MS media influenced the in vitro sweet kaffir lime seeds' growth. Sweet kaffir lime seeds that were given 4 ppm BAP grew buds faster with more percentage of shoot growth, the number of shoots, and a number of leaves than the seeds without BAP adduction.

The Relationship between Transpiration and Calcium Fertilization on Mangosteen (Garcinia mangostana L.) Seedlings Ajmir Akmal; Edi Santosa; Roedhy Poerwanto; Ismadi Yunus; Rd. Selvy Handayani
Journal of Tropical Horticulture Vol 1, No 1 (2018): October 2018
Publisher : Indonesian Society for Horticulture (Perhimpunan Hortikultura Indonesia Komisariat Aceh)

The availability of nutrients in plants depends on the ability of plants to absorb nutrients from the soil. One of the processes absorbed by plants is by the presence of the transpiration process. Good transpiration will provide enough nutrients for plants which increase the productivity and quality of mangosteen. One of the quality standards of mangosteen fruit for export quality is the free from yellow sap contamination. It can be possible that transpiration can improve the quality of mangosteen fruit by applying fertilization. In this study Ca fertilization was carried out on mangosteen seeds, where this study aims to investigate the effectiveness of Ca uptake by mangosteen plants with the level of water loss by transpiration. This research was conducted in November 2016-March 2017 at the Leuwikopo Experimental Garden, Bogor Agricultural University. Morphological observations were carried out in its Experimental Garden and the Postharvest Laboratory whileanalysis of calcium content of plant tissue was done at the Department of Agronomy and Horticulture, Bogor Agricultural University. Data obtained then were analyzed using ANOVA test. If the results were significant, Duncan Multiple Range Test (DMRT) was then tested at 0.05 probability level. The results revealed that the transpiration rate of mangosteen plants from several treatments showed a significant difference, the transpiration rate was higher, especially in fertilized plants. The size of the 12th leaf, plants treated with fertilizer have a larger size than the treatment without fertilization. Fertilizing and providing enough water can maintain the growth of the plants.

WOMEN EMPOWERMENT THROUGH MUSHROOM CULTIVATION AND PROCESSING IN ACEH UTARA Rd. Selvy Handayani; Ismadi; Khaidir; Dewi Sartika Aryani; Zurrahmi Wirda; Usnawiyah
Global Science Society Vol 4 No 1 (2022): Global Science Society (GSS) Jurnal Ilmiah Pengabdian Kepada Masyarakat
Publisher : LPPM dan PM Universitas Samudra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33059/gss.v4i1.4512

Training in mushroom cultivation has been chosen to be conducted in this community empowerment program, as we believe that this training can support the community in Desa Mesjid, Syamtalira Aron District Aceh Utara, not only to increase their income, but also can enrich their knowledge in serving healthier diets for their family. The main objective of this program was to increase community income and to enrich the knowledge about healthy foods by teaching them how to cultivate oyster mushroom. The results showed that the community was enthusiastic and satisfied with knowledge they obtained from the training. Ninety percent of them said it was a great, informative and useful activity. There is an increase in their knowledge about mushroom cultivation from 23% to 78%, and also how to process the mushroom into crispy fried mushroom and mushroom nugget and then to market them from 23% to 73%.

Pengaruh Letak Posisi Eksplan dan Sitokinin Pada Perkecambahan Biji Manggis (Garcinia mangostana L.) Lokal Aceh Secara in-Vitro Rd Selvy Handayani; Maisura Maisura; Astia Rizki
Agrium Vol 14, No 2 (2017)
Publisher : Faculty of Agriculture, Universitas Malikussaleh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29103/agrium.v14i2.874

The application of modern biotechnology is needed in order to enhance the genetic diversity of mangosteens. The application of tissue culture on mangosteens is an important thing to do to support the biotechnology program of mangosteen plants. The purpose of this research is to reveal the influence of location of explants and Benzylaminopurine (BAP) concentration on germination of local Acehnese mangosteens in vitro. This research was conducted at the Laboratory of tissue culture of the Faculty of Agriculture, Malikussaleh University from March to May 2017. The study used an environmental design in the form of Completely Randomized Design (CRD) with two factors. The first factor is the location of explant position is abaxial (L1) and adaxial (L2). The second factor was the concentration of BAP 0 mg / L (B0), 2.5 mg / L (b1), and 5 mg / L (B2). The results showed that the position of eksplan gave an effect on mangosteen germination in vitro for its shoot length variables. The explant positions that had the best effect on the growth of in vitro mangosteen sprouts were adaxial. BAP concentration had an effect on germination of mangosteen in vitro for its bud growth and life percentage. 2.5 ppm of BAP concentration was the best concentration for mangosteen seed growth in vitro. There was no interaction between eksplan position and BAP concentration in all observed variables

Inventarisasi dan Analisis Risiko Gulma Asing Invasif Pada Lahan Pertanian di Sawang Aceh Utara Nanda Firmansyah; Baidhawi Baidhawi; Khusrizal Khusrizal; Rd Selvy Handayani
Agrium Vol 16, No 2 (2019)
Publisher : Faculty of Agriculture, Universitas Malikussaleh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29103/agrium.v16i2.5866

Kehadiran gulma asing invasif (GAI) memberikan peluang terjadinya peristiwa yang tidak dikehendaki sebagai akibat dari tindakan pengelolaan tanaman introduksi yang menguasai dan menyebar pada lahan pertanian. Penelitian ini bertujuan untuk menginventarisasi dan menganalisis resiko spesies GAI pada lahan pertanian di Sawang Aceh Utara. Titik lokasi pengambilan sampel dilakukan pada beberapa tipe penggunaan lahan (TPL) yaitu kebun/tegalan, huma/ladang, sawah non-irigasi, sawah irigasi, perkebunan, padang rumput, hutan rakyat, dan kolam/tambak. Jenis GAI dikoleksi secara langsung dari lapangan dengan metode jelajah, dan dianalisis menggunakan sistem scoring mengacu pada pedoman analisis risiko GAI dari KLHK (Pusat Penelitian dan Pengembangan Hutan, Kementerian Lingkungan Hidup dan Kehutanan) dan FORIS (Forests in Southeast Asia) Indonesia. Hasil kajian memperlihatkan terdapat 659 individu GAI yang berasal dari 36 spesies dan 14 famili. Famili yang memiliki spesies GAI terbanyak adalah famili Poaceae. Jenis gulma terbanyak berasal dari jenis gulma berdaun lebar sebanyak 20 spesies, sedangkan jumlah spesies GAI terbanyak yaitu spesies Rhynchospora colorata (L.) dengan jumlah 66 individu. Kategori risiko sangat tinggi disebabkan oleh spesies Axonopus compressus, Chromolaena odorata dan Paspalum conjugatum pada lahan tegalan/kebun, spesies Cyperus distans dan Rhynchospora colorata pada lahan ladang/huma, spesies Dactyloctenium aegyptium pada lahan padang rumput dan spesies gulma Panicum maximum pada lahan sawah irigasi.Kategori risiko sedang terdapat spesies gulma Ageratina adenophora pada lahan perkebunan dan spesies gulma Ageratum conyzoides pada lahan sawah non irigasi.Kategori risiko rendah terdapat spesies gulma Digitaria fuscescens pada lahan hutan rakyat dan Heliotropium indicum pada lahan perkebunan.

Co-Authors Agus Purwito Ajmir Akmal Astia Rizki Baidhawi Baidhawi Dewi Sartika Aryani Edi Santosa Endri Irawan Eva Yulia Hafifah Hafifah Hafifah Hafifah Ira Handayani Ira Handayani Ira Handayani Ismadi Ismadi Khaidir Khaidir Khusrizal Khusrizal Laila Nazirah M Sayuti Mahin Solihuddin Maisura Maisura Mira Agustina Muhamad Yusuf Muhammad Rusdi Muhammad Suhendra Muksalmina Muksalmina Nadia Tillah Nanda Firmansyah Nanda Firmansyah Nasrun, Mahdalena Ni Luh Ari Yusasrini Nurisna Baiti Paiman Paiman ROEDHY POERWANTO Sobir Sobir Tri Muji Ermayanti Usnawiyah Usnawiyah Usnawiyah Zurrahmi Wirda

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