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α-SMA Expression Increased Over Cell Passages and Decreased by Exogenous TGF-β1, In Vitro Studies on Myofibroblast Derived from Orbital Socket Contracture Shintiya Dewi, Debby; Chairinnisa, Elsa Safira; Sujuti, Hidayat; Lirawati, Diana; Hernowati, Tinny Endang
Journal of Tropical Life Science Vol 8, No 2 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.08.02.15

Abstract

α-smooth muscle actin (α-SMA), a marker of myofibroblast, induces cytoskeleton reorganization, increases contractility and stimulates cell migration in TGF-β1 induced stress fibers. The aims of the present study were to determine the level of α-SMA expression and morphological cell changes in different passages of myofi-broblasts with varied TGF-β1 concentrations. Myofibroblast cell cultures were derived from fibrotic tissues of fourth degree socket contracture. The α-SMA expression level was measured in myofibroblast cultures pas-sage I, II, and III with and without 10 ng/mL TGF-β1, and in passage III with 2.5; 5; 10; and 20 ng/mL TGF-β1. Results: The levels of α-SMA expression level in passage I to III were I 31.42 ± 3.4; 40.34 ± 8.14 and 56.37 ± 7.57, respectively. Addition of 10 ng/mL TGF-β1 into passage I-III myofibroblast cultures resulted in α-SMA expression level of 31.24 ± 2.93; 36.81 ± 6.09; and 14.29 ± 2.72, respectively. Myoblasts passage III showed the lowest α-SMA expression level following exposure to TGF-β1 10 ng/mL (22.37 ± 12.86) and highest without TGF-β1 (48.34 ± 13.36), however no morphological changes detected. α-SMA expression level increased with cell passages, decreases with addition of TGF-β1 while not affecting morphology of myofibroblast derived from the orbital socket contracture.
Immunohistochemistry Evaluation of TGF-β1, SOX-9, Type II Collagen and Aggrecan in Cartilage Lesions Treated with Conditioned Medium of Umbilical Cord Mesencyhmal Stem Cells in Wistar Mice (Rattus novergicus) Soetjahjo, Bintang; Hidayat, Mohammad; Sujuti, Hidayat; Fibrianto, Yuda
Journal of Tropical Life Science Vol 8, No 1 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1061.898 KB) | DOI: 10.11594/jtls.08.01.05

Abstract

Currently, umbilical cord mesenchymal stem cells have the potential to be used as treatment options for any cartilage lesion. This research aimed to evaluate the effects of conditioned medium from umbilical cord mesenchymal stem cells (UC-MSC) on damaged cartilage through the expression of proteins TGF-β1, SOX-9, type II collagen and aggrecan, which are known to be related to chondrogenesis. UC-MSC were isolated from 19-days-pregnant Wistar mice and were cultured using the standard procedure to obtain 80% confluence. Subsequently, the culture was confirmed through a microscopic examination that was driven to be an embryoid body to obtain a pre-condition medium. This research utilized 3-month-old male Wistar mice and was categorized into 6 groups (3 control and 3 treatment groups). Each animal had surgery performed to create a femur condyle cartilage defect. The treatment groups were administered a dose of stem cells at 1 mL/kg. Next, immunohistochemical (IHC) staining was performed to examine the expression of TGF-β1, SOX-9, type II collagen and aggrecan in the 2nd, 3rd, and 4th month of evaluation. The results were analyzed statistically using ANOVA test. For each of the treatment groups, there was increased expression (p < 0.05) in all proteins TGF-β1, SOX-9, type II collagen and aggrecan when compared with control groups at the 2nd, 3rd, and 4th month of evaluation. Pre-conditioned medium from UC-MSC potentially increases the expression of TGF-β1, SOX-9, type II collagen and aggrecan in the damaged cartilage of Wistar mice.
Plasma Glucagon-Like Peptide-1 and Cholecystokinin Responses to Fast Food in Healthy-Weight and Obese Men Handayani, Dian; Putri, Dianita Setya Pradita; Sujuti, Hidayat; Andarini, Sri; Rahmawati, Widya; Kusumastuty, Inggita; Harti, Leny Budhi; Sabrina, Nindy
Jurnal Kedokteran Brawijaya Vol 31, No 1 (2020)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (59.1 KB) | DOI: 10.21776/ub.jkb.2020.031.01.14

Abstract

Satiety hormones play a role in obesity metabolism. The satiety response to similar nutrients in food in healthy and obese men remains undefined. The research was aimed to determine the satiety response differences by comparing the effect of isocaloric fast-food consumption on reducing appetite-related gut hormones, such as glucagon-like fullness ratings and both GLP-1 and CCK among healthy and obese men. Respondents were given an isocaloric fast food, then GLP-1 and CCK levels were measured using enzyme-linked immunosorbent assay (ELISA). Visual analogue scale (VAS) form was used for hunger and fullness ratings of the subjects. The difference level of GLP-1, CCK, and VAS between groups were measured by t-test. The correlation between VAS hunger and fullness rating was measured by Pearson. Plasma hormone levels in 16 obese and 16 healthy-weight respondents were assessed before eating and at 30, 60, and 120 minutes after consumption. In obese men, GLP-1 levels were significantly higher than those in healthy-weight men at 60 and 120 minutes, while healthy-weight men had significantly higher CCK levels than those of obese men over time (all p<0.05). The total area under the curve (AUC) for GLP-1 was significantly higher for obese men than for healthy-weight men, while the AUC for CCK was significantly higher for healthy-weight men than for obese men. Obese men have higher plasma GLP-1 levels and lower plasma CCK than healthy men indicates that those respondents were experiencing glucose intolerance and leptin alteration. The hormonal systems that may contribute to the development of obesity need further investigation.
Rho- Kinase Inhibitor Y-27632 Decreases the Thickness of Trabecular Meshwork in Juvenile Rats Mod-el Injected with Sodium Hyaluronate Vierlia, Wino Vrieda; Wulandari, Lely Retno; Sujuti, Hidayat; Effendi, Ma'sum
Journal of Tropical Life Science Vol 8, No 2 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.08.02.13

Abstract

Most glaucoma drugs lower the intraocular pressure (IOP) by decreasing the aqueous humor production and increasing the outflow through uveoscleral pathway. None of these drugs work mainly on increasing outflow through the trabecular pathway. Consequently, the experiment to develop glaucoma drugs directly target at the trabecular outflow pathway is highly required. The purpose of this study is to reveal the effect of rho-kinase inhibitor Y-27632 on the thickness of trabecular meshwork in juvenile rats model injected with sodium hyalu-ronate.  This study was an experimental study with posttest only control group design. Twenty-four rats were included in this study. Each eye of the rat would be considered as one sample. Samples were divided into 6 groups, negative control group, positive control I group with intracameral sodium hyaluronate injection, posi-tive control II group with topical Y-27632 10 mM, and three experimental groups with intracameral injection of sodium hyaluronate and Y-27632 10-1 mM, 1 mM, and 10 mM respectively. After the procedures all rats were sacrificed and enucleated. Trabecular meshwork tissue was stained with Hematoxilene-Eosin and evalu-ated under 400× microscopic magnification. Quantitative measurements were taken using computerized image analysis with dot slide program.  There were significant statistic differences among the positive control I group and the experimental groups (p-value < 0.05) as well as the positive control II group and the experi-mental groups (p-value < 0.05). The highest mean of decreasing trabecular meshwork thickness was noted in the group given by sodium hyaluronate and Y-27632 10 mM with value of 118.42 µm. There was decreasing thickness of trabecular meshwork due to the effect of rho- kinase inhibitor Y-27632 in juvenile rats injected with sodium hyaluronate.
Correlation of Malondialdehyde (MDA) and C-reactive Protein (CRP) Level to Neurodevelopmental Outcome in Children After the Episode of Convulsive Type Status Epilepticus Restuningwiyani, Sintha; Ariani, Ariani; Sujuti, Hidayat; Rahayu, Masruroh; Subandiyah, Krisni
Journal of Tropical Life Science Vol 8, No 3 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (229.772 KB) | DOI: 10.11594/jtls.08.03.07

Abstract

Refractory and mortality associated with status epilepticus (SE) were correlated with the degree of inflammation-induced neuronal cell death. This study was aimed to investigate the correlation of oxidative stress (Malondialdehyde, MDA) and inflammation (C-reactive protein, CRP) process with neurodevelopmental outcome in children after the episode of convulsive type SE. This study was designed as cross sectional which included 26 convulsive type SE subjects and 15 control subjects. MDA level was measured by thiobarbituric acid (TBA) method, while CRP level was measured by ELISA method. Neurodevelopmental outcome was measured by Bayley-III Scale of Infant and Toddler Development 3 month after the convulsive type SE episode. Results showed that both MDA (independent t-test, p &lt; 0.05) and CRP (Mann-Whitney test, p &lt; 0.05) level was significantly higher in convulsive type SE group as compared to control group. Further analysis also showed that MDA (Spearman correlation test, p = 0.000, r = 0.756) and CRP (Spearman correlation test, p = 0.000, r = 0.835) level was positively correlated with convulsive type SE. In convulsive type SE group, MDA level was negatively correlated with neurodevelopmental outcome but CRP was not. We concluded that MDA level was negatively correlated with neurodevelopmental outcome in children with convulsive type SE, but CRP was not.
Serbuk Daun Kelor Menurunkan Derajat Perlemakan Hati dan Ekspresi Interleukin-6 Hati Tikus dengan Kurang Energi Protein Dwi, Agustiana; Endang, Tinny; Sujuti, Hidayat
Jurnal Kedokteran Brawijaya Vol 26, No 3 (2011)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1216.547 KB) | DOI: 10.21776/ub.jkb.2011.026.03.1

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Kurang energi protein (KEP) merupakan salah satu bentuk malnutrisi karena rendahnya asupan gizi. Pada KEP gambaran histopatologi  liver  akan  menunjukkan  perlemakan  hati  yang  bereaksi  dengan  radikal  peroksidasi  membentuk  lipid peroksidasi  yang  meningkatkan  ekspresi  Interleukin6  (IL-6).  Penelitian  ini  dilakukan  untuk  membuktikan  bahwa  konsumsi serbuk daun kelor dapat  menurunkan derajat  perlemakan hati  dan ekpresi IL-6  pada KEP .  Penelitian dilakukan dengan desain post test only control group, pada enam kelompok (diet normal, diet rendah protein, diet rendah protein dengan empat  perlakuan  dosis  kelor  yaitu  180  mg,  360  mg,  720  mg,  1440  mg.  Diet  rendah  protein  diberikan  dalam  2  bulan kemudian    dilanjutkan  dengan  1  bulan  diet  normal  yang  ditambahkan  serbuk  daun  kelor .    Hasil  uji  One  Way  ANOVA menunjukkan bahwa pemberian serbuk daun kelor pada semua dosis dapat menurunkan derajat perlemakan hati dan ekspresi  IL-6.   Pada  dosis  720  mg/hari,  derajat  perlemakan  hati  dan  ekspresi  IL-6  menurun hingga  sama  dengan  kelompok normal. Dapat disimpulkan bahwa pemberian serbuk daun kelor pada KEP dapat menurunkan derajat perlemakan hati dan  ekspresi  IL-6.Kata  Kunci:  Ekspresi  IL-6,  kurang  energi  protein,  perlemakan  hati,  serbuk  daun  kelor
PENGARUH KADAR GLUKOSA TERHADAP EKSPRESI PROTEIN AdhO36 BAKTERI Salmonella Typhi Winarsih, Sri; Sujuti, Hidayat; Yasmin, Aulia
Majalah Kesehatan FKUB Vol 4, No 3 (2017): MAJALAH KESEHATAN FAKULTAS KEDOKTERAN
Publisher : Faculty of Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (612.515 KB) | DOI: 10.21776/ub.majalahkesehatan.2017.004.03.1

Abstract

Demam tifoid yang disebabkan oleh Salmonella Typhi merupakan salah satu penyakit infeksi terbanyak penyebab rawat inap penderita di Indonesia. Sehingga diperlukan penelitian tentang faktor-faktor yang mempengaruhi patogenesitas dari S.Typhi salah satunya adalah kadar glukosa. S. Typhi diketahui memiliki molekul adhesin pada bagian  outer membrane protein (OMP) dengan berat molekul 36 kDa atau disebut juga AdhO36. Molekul adhesin ini berfungsi sebagai perlekatan bakteri pada sel hospes. Pada penelitian ini ingin diketahui pengaruh kadar glukosa terhadap ekspresi protein AdhO36 OMP S. Typhi. Perlakuan glukosa diberikan pada lima kelompok bakteri yaitu konsentrasi 40 mg/100mL, 80 mg/100mL, 160 mg/100mL, 240 mg/100mL dan 320 mg/100mL. Hasil elektroforesis OMP pada lima kelompok bakteri tersebut menunjukkan peningkatan ketebalan pita protein, yaitu semakin tinggi kadar glukosa akan  semakin tebal protein yang  terekspresi. Ketebalan pita protein OMP 36 kDa dibaca menggunakan program Corel Photo Paint 11. Hasil analisis statistik menunjukkan perbedaan yang tidak bermakna (ANOVA, p = 0,559), namun secara deskriptif terlihat adanya peningkatan ketebalan protein OMP 36 kDa pada perlakuan konsentrasi glukosa yang makin besar. Kesimpulan penelitian ini adalah peningkatan kadar glukosa cenderung akan meningkatkan ekspresi protein AdhO36 S. Typhi. Kata kunci : AdhO36, Kadar Glukosa, OMP, Salmonella Typhi
Regulasi Adipogenesis oleh mTORC1 melalui Jalur ST A T3 Triawanti, Triawanti; Indra, M Rasjad; Tjokroprawiro, Askandar; Sujuti, Hidayat
Jurnal Kedokteran Brawijaya Vol 27, No 3 (2013)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1125.568 KB) | DOI: 10.21776/ub.jkb.2013.027.03.1

Abstract

Obesitas  merupakan  suatu  penyakit  kelebihan  massa  lemak  tubuh  yang  mempunyai  efek  merugikan  bagi  kesehatan.  Pada saat  ini  proses  adipogenesis  menjadi salah  satu  target  terapi  obesitas.  Salah  satu  jalur  yang  diduga  teraktivasi  pada  proses adipogenesis  adalah  melalui aktivasi  ST A T3  yang  salah  satu  jalur  hulunya  melalui protein  mammalian target  of rapamycin complex 1 (mTOR). Penelitian ini bertujuan untuk membuktikan proses adipogenesis melalui jalur ST A T3 yang diaktivasi oleh  mTORC1.  Penelitian  ini  merupakan  penelitian  eksperimental  dengan  rancangan  post  test  only  control.  Untuk penghambatan  mTORC1  digunakan  rapamycin  dan  penghambatan  ST A T3  digunakan  inhibitor  ST A T3  peptide.  Subjek penelitian  adalah  kultur  primer  sel  preadiposit  yang  diambil  dari  lemak  viseral  tikus  putih  Rattus  norvegicus.  Setelah  kultur sel preadiposit dinilai konfluen minimal 70-80% dilakukan induksi diferensiasi dan dibagi menjadi  4 kelompok yakni (K) kontrol  (A):   diberi rapamycin  10  nM,  (B):  diberi  inhibitor  ST A T3  100  µM (C):  diberi  inhibitor  ST A T3  100  µM dan   rapamycin10  nM.  Parameter  yang  diukur  adalah  aktivasi  p70S6K1,  ST A T3,  ekspresi  C/EBPδ,  aktivitas  enzim  Glyserol-3-fosfodehidrogenase  (GPDH)  pada  hari  ke-2,  ke-4  dan  ke-6  serta  gambaran  morfologis  sel  adiposit.  Analisis  statistik menggunakan uji ANOVA, Duncan dan korelasi Pearson dengan tingkat kepercayaan 95%. Hasil penelitian membuktikan terjadi  penghambatan  proses  adipogenesis  karena  penghambatan  aktivasi  p70S6K1,  dan  ST A T3  oleh  rapamycin  dan inhibitor ST A T3.
Isolat EGCG Teh Hijau Klon GMB4 Menurunkan Ekspresi Protein Faktor Transkripsi C/EBPα dan Kadar Leptin pada Kultur Sel Preadiposit Visceral Tikus Wardhana, Analis W; Ratnawati, Retty; Sujuti, Hidayat
Jurnal Kedokteran Brawijaya Vol 27, No 4 (2013)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (633.465 KB) | DOI: 10.21776/ub.jkb.2013.027.04.6

Abstract

Isolat golongan senyawa katekin teh hijau (Camelia sinensis) klon GMB4 dapat dikembangkan sebagai agen terapeutik potensial untuk obesitas. Penelitian ini ditujukan untuk mengetahui efek isolat EGCG teh hijau klon GMB4 terhadap ekspresi protein faktor transkripsi C/EBPα dan terhadap kadar protein leptin pada kultur preadiposit viseral tikus. Metode ELISA digunakan untuk mengukur kadar leptin dan immunositokimia untuk ekspresi protein faktor transkripsi C/EBPα. Kultur sel preadiposit viseral tikus yang diisolasi dari tikus Rattus norvegicus Wistar yang ditumbuhkan dalam medium adipogenik dan selanjutnya dipapar dengan isolat golongan senyawa katekin teh hijau (Camelia sinensis) klon GMB4 dengan konsentrasi 0, 50 µM, 100 µM, dan 200 µM. Ekspresi C/EBPα paling rendah terdapat pada konsentrasi isolat EGCG 200 µM. Kadar leptin menurun seiring dengan dosis EGCG yang dinaikkan, sehingga kadar leptin berbanding terbalik dengan dosis EGCG. Kadar leptin terendah pada pemaparan EGCG dengan dosis 200 µM. Dapat disimpulkan bahwa isolat EGCG teh hijau klon GMB4 secara signifikan dapat menurunkan ekspresi  protein faktor transkripsi  C/EBPα pada kultur preadiposit viseral tikus pada konsentrasi 200 µM dan menurunkan kadar protein leptin pada kultur preadiposit viseral tikus pada konsentrasi 50 µM, 100 µM, dan 200 µM.Kata Kunci: C/EBPα, EGCG, GMB4, leptin, sel preadiposit, teh hijau
Efek Ekstrak Etanol Daun Kelor (Moringa oleifera) terhadap Nuclear Factor Kappa Beta (NF-kB) Aktif dan Apoptosis Cell Line Kanker MCF-7 Andjani, Nabila; Sujuti, Hidayat; Winarsih, Sri
Majalah Kesehatan FKUB Vol 3, No 4 (2016): MAJALAH KESEHATAN
Publisher : Faculty of Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (661.165 KB)

Abstract

Aktivasi NF- ĸB, suatu parameter apoptosis kanker, menyebabkan induksi beberapa fungsi seluler antara lain proliferasi sel meningkat dan penurunan apoptosis. Daun kelor (Moringa oleifera) secara ayurveda telah terbukti dalam mencegah penyakit leukemia dan skin papilomagenesis. Tujuan penelitian ini ialah membuktikan bahwa pemberian ekstrak daun kelor menurunkan aktivitas sel kanker payudara MCF-7. Penelitian ini menggunakan true experimental in vitro design dengan sel MCF-7. MTT Assay dilakukan dengan menggunakan dosis terapi dari rentang 7,1825–20000 µg/ml untuk menentukan IC50 pada sel MCF-7. IC50 yang didapatkan adalah dosis 2200 µg/ml. Immunositokimia dan TUNEL assay dengan kelompok kontrol negatif, kelompok perlakuan dosis 1100, 2200, 4400 µg/ml. Terdapat perbedaan signifikan yang lebih rendah pada perlakuan dosis 2200 µg/ml (p = 0,000) dan dosis 4400 µg/ml (p = 0,000) dibandingkan kontrol tanpa terapi pada aktivitas NF-ĸB. Besar persentase aktivitas NF-ĸB pada dosis 2200 µg/ml  adalah 31,651% dan 4400 µg/ml  adalah 36,575%. Namun, apoptosis pada MCF-7 tidak dapat dilihat. Kesimpulan dari penelitian ini ialah ekstrak daun kelor dapat menurunkan aktivitas sel MCF-7, ekstrak daun kelor dapat menurunkan jumlah NF-ĸB aktif pada dosis 2200 dan 4400 µg/ml. Dosis IC50 yang diperoleh adalah 2200 µg/ml, dan peristiwa apoptosis tidak dapat diamati. Kata kunci: Daun kelor (Moringa oleifera), Kanker payudara, MCF-7, NF kB.
Co-Authors A.A. Ketut Agung Cahyawan W Abdalrauf A Mahmud Yousif, Abdalrauf A Mahmud Abdussalam Ashour, Abdussalam Affandi, Haykal Agung Prasetyo Wibowo Agustiana Dwi Agustina Tri Endharti Ala Shukri Eshami, Ala Shukri Analis W Wardhana Anik Puryatni Anisak, Siti Ariani Ariani Askandar Tjokroprawiro Aulia Yasmin, Aulia Bagus Hermansyah Bayu Kurniawan Burhan, Niniek Chabibi, Mochamad Chabibi Chairinnisa, Elsa Safira Dalhar, Mochamad Dian Handayani Diana Lyrawati Didi Candradikusuma Dwi Jayasa, Pande Made Dwi Yuni Nur Hidayati Edi Widjajanto Effendi, Ma'sum Eko Sulistijono Erma Sulistyaningsih Ery Olivianto Fibrianto, Yuda Gerry Gunawan, Gerry Hanif Hanif Hara, Kenta HARI PURNOMO Harijono Harijono Hariwati Hariwati Helilusiatiningsih, Nunuk Huda Rohmawati Husna, Ully Inggita Kusumastuty Juniartha, Putu Kanthi Permaningtyas Tritisari, Kanthi Permaningtyas Karyono Mintaroem Karyono mitaroem, Karyono Krisni Subandiyah Lely Retno W Lely Retno Wulandari Leny Budhi Harti Lirawati, Diana Loeki Enggar Fitri M Rasjad Indra Maimun Z Arthamin, Maimun Z Masafumi Matsuo Masdar Muid Masruroh Rahayu, Masruroh Miftahu Soleh Moch. Aris Widodo, Moch. Aris Mochammad Dalhar Mohammad Hidayat Muhammad Rasjad Indra Nabila Andjani, Nabila Nisrina Nur Afina, Nisrina Nur Pande Made Dwijayasa Permatasari, Happy Kurnia Prima Wijayanti, Prima Primawardani, Putri Puryatni, Anik - Putri, Dianita Setya Pradita Raisa, Neila Restuningwiyani, Sintha Retnani, Diah Prabawati Retty Ratnawati Sabrina, Nindy Salim, Irfan Agus Samsul Arifin Santoso, Giovani Anggasta Setiyaningsih, Fera Yuli Setyawati Soeharto, Setyawati Shintiya Dewi, Debby Shofiyah, Fita Simon Bambang Widjanarko Siti Lintang Kawuryan Soetjahjo, Bintang Sri Andarini Sutrisno, Sutrisno Syahrul Chilmi, Syahrul Syarifah Rohaya Tatit Nurseta Tinny Endang Tinny Endang Hernowati Tokunaga, Chiharu Triawanti Triawanti Tuda, Josef Sem Berth Umar Zein Vierlia, Wino Vrieda Widya Rahmawati Yonezawa, Kazuyoshi Yoshino, Ken-ichi Yudanto, Hendy Setyo YUNIANTA YUNIANTA Zuly Vita Aulya, Zuly Vita