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All Journal CAKRA KIMIA (Indonesian E-Journal of Applied Chemistry) Jurnal Kimia (Journal of Chemistry) Buletin Udayana Mengabdi Majalah Obat Tradisional Jurnal Media Sains Jurnal Ilmu Pendidikan Indonesia Journal of Global Pharma Technology
I M, Sukadana
Program Studi Kimia FMIPA Universitas Udayana, Bukit Jimbaran, Bali
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Electrical & Electronics Engineering Health Professions Immunology & microbiology Mathematics Medicine & Pharmacology Physics Public Health

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SENYAWA ANTIBAKTERI BIS (2-ETILHEKSIL) ESTER DAN TRITERPENOID DALAM EKSTRAK n-HEKSANA DAUN TEMPUYUNG (Sonchus arvensis L.) Sukadana, I Made; Santi, Sri Rahayu
Majalah Obat Tradisional Vol 16, No 1 (2011)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (133.815 KB) | DOI: 10.14499/mot-TradMedJ16iss1pp%p

Abstract

Telah dilakukan isolasi senyawa antibakteri dari ekstrak kental n-heksana daun tempuyung (Sonchus Arvensis L.). Sebanyak 9,15 g ekstrak kental n-heksana hasil partisi menunjukkan aktif antibakteri dengan diameter zona hambat sebesar 9 mm  terhadap Staphylococcus aureus dan 8 mm terhadap Escherichia coli pada konsentrasi uji 1000 ppm. Pemisahan ekstrak kental n-heksana menggunakan teknik kromatografi kolom diperoleh 5 fraksi dengan pola pemisahan yang berbeda, yaitu fraksi F1, F2, F3, F4, dan F5 yang masing-masing mengandung jumlah komponen senyawa yaitu 6, 5, 4, 2, dan 1 senyawa. Hasil uji kelima fraksi ini terhadap bakteri Staphylococcus aureus dan Escherichia coli menunjukkan bahwa fraksi F2 dan F4, positif menghambat pertumbuhan bakteri Escherichia coli dan Staphylococcus aureus. Fraksi F3 hanya positif menghambat pertumbuhan bakteri Escherichia coli sedangkan fraksi F5 hanya positif menghambat pertumbuhan bakteri Staphylococcus aureus. Pemisahan lebih lanjut terhadap fraksi aktif antibakteri (F4) menggunakan teknik kromatografi lapis tipis (KLT) preparatif, menghasilkan 3 fraksi (fraksi F4.1, F4.2, dan F4.3), dimana fraksi F4.1 menunjukkan fraksi yang paling aktif menghambat pertumbuhan bakteri uji dengan diameter zona hambat sebesar 9 mm terhadap Staphylococcus aureus dan 11 mm terhadap Escherichia coli pada konsentrasi uji 100 ppm. Hasil identifikasi terhadap fraksi F4.1 (isolat) menggunakan Kromatografi Gas-Spektroskopi Massa (KG-SM) dan pendekatan ”database” WILEY7 menunjukkan bahwa isolat merupakan campuran 2 komponen senyawa yaitu bis (2-etilheksil) ester dan kemungkinan senyawa baru golongan triterpenoid dengan rumus molekul C32H66O6 .  
Aktivitas Antimakan Daun Tenggulun (Protium javanicum Burm. F.) terhadap Ulat Kubis Plutella xylostella Sukadana, I Made; Santi, Sri Rahayu; Monikayani, Ni Wayan
Jurnal Media Sains Vol 2, No 2 (2018): JURNAL MEDIA SAINS
Publisher : LPPM Universitas Dhyana Pura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36002/jms 3.v2i2.428

Abstract

ABSTRAKDaun tenggulun (Protium javanicum Burm. F.) secara tradisional telah dimanfaatkan sebagai obat dan insektisida seperti repelan dan antimakan terhadap larva Epilachna sparsa suatu bioindikator universal terhadap hama ulat sehingga dapat dikembangkan untuk ulat lain seperti ulat kubis. Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi golongan senyawa aktif antimakan daun tenggulun (Protium Javanicum Burm. F.) terhadap ulat kubis Plutella xylostella. Hasil maserasi 1000 g serbuk kering daun tenggulun dengan metanol menghasilkan 42,7218 g ekstrak kental metanol. Ekstrak ini menunjukkan aktivitas antimakan 71,01% pada konsentrasi 0,1% (b/v) yang selanjutnya dipisahkan dengan n-heksana, etil asetat, dan air. Ekstrak n-heksana, etil asetat, dan air pada konsentrasi 0,1% (b/v) menunjukkan aktivitas antimakan berturut-turut 30,56%, 84,24%, dan 5,05%. Pemurnian ekstrak aktif etil asetat dilakukan menggunakan kromatografi kolom dengan fase diam silika gel dan fase gerak etil asetat-n-heksana (1:1) menghasilkan tiga fraksi. Fraksi B yang menunjukkan aktivitas antimakan paling tinggi yaitu 87,26% pada konsentrasi 100 ppm dan relatif murni secara KLT. Hasil identifikasi menunjukkan isolat merupakan senyawa golongan triterpenoid alkohol dengan gugus fungsi OH, CH3, CH2, CH, C=O, -Vis pada 282,5 nm (???*), 403 nm (n??*), dan 437,5 nm (n??*).Kata kunci: Protium javanicum Burm. F., tenggulun, antimakan, Plutella xylostella, triterpenoid alkoholABSTRACTTenggulun leaves (Protium javanicum Burm. F.) have been used traditionally as a medicine and insecticide such as repellant and antifeedant for Epilachna sparsa larva a universal bioindicator to larva that can be developed for other larva such as cabbage larva. This research aimed to isolate and identify antifeedant active compounds from tenggulun leaf (Protium javanicum Burm. F.) to cabbage larva Plutella xylostella. Dry powder of tenggulun leaves extracted with methanol was obtained 42,7218 g of methanol crude extract. This extract showed 71,01% antifeedant activity at 0,1% (w/v) subsequently separated by partition to obtain n-hexane, ethyl acetate, and water extracts. Extract of n-hexane, ethyl acetate, and water at concentration 0,1% (w/v), showed the antifeedant activity of 30,56%, 84,24%, and 5,05%. Purification of etil acetat extract using silica gel column chromatography and mobile phase ethyl acetate-n-hexane (1:1) yielded three fractions. Fraction B is the most active which showed 87,26% antifeedant activity at 100 ppm was found relatively pure with thin layer chromatography. The antifeedant active isolate was identified as triterpenoid alcohol group of compounds which have functional group OH, CH3, CH2and gives UV-Vis rays at 282,5 nm (???*), 403 nm (???*), and 437,5 nm (n??*).Keywords: Protium javanicum Burm. F., tenggulun, antifeedant, Plutella xylostella, triterpenoid alcohol
TOKSISITAS EKSTRAK DAN ISOLAT RUMPUT LAUT Eucheuma spinosum Melisa Anggraini; I Made Dira Swantara; I Made Sukadana
CAKRA KIMIA (Indonesian E-Journal of Applied Chemistry) Vol 9 No 1 (2021): Cakra Kimia (Indonesian E-Journal of Applied Chemistry)
Publisher : Magister Program of Applied Chemistry, Udayana University, Bali-INDONESIA

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Abstract

ABSTRAK: Rumput laut memiliki peran sebagai penjaga kestabilan ekosistem laut, tempat hidup dan perlindungan bagi biota lain, serta memiliki potensi ekonomis sebagai bahan baku kesehatan. Beberapa spesies rumput laut dapat dimanfaatkan untuk kesehatan karena mengandung senyawa kimia yang memiliki aktivitas biologis. Eucheuma spinosum mengandung senyawa metabolit sekunder seperti flavonoid, triterpernoid, dan steroid. Penelitian ini dilakukan untuk mengetahui lebih lanjut mengenai toksisitas dan kandungan senyawa aktif yang terdapat dalam E. spinosum. Uji toksisitas dilakukan menggunakan metode Brine Shrimp Lethality Test (BSLT) dan identifikasi senyawa dilakukan dengan uji fitokimia. Proses ekstraksi 6 kg E. spinosum segar dilakukan dengan metode maserasi menghasilkan 12.78 g ekstrak kasar. Selanjutnya ekstrak kasar dipartisi menghasilkan 5.84 g ekstrak heksana, 0.54 g ekstrak kloroform dan 0.52 g ekstrak n-butanol yang masing-masing memiliki nilai LC50­ berturut-turut sebesar 75.199; 115.577; dan 549.499 ppm. Ekstrak n-heksana menunjukkan hasil positif terhadap alkaloid dan steroid. Pemisahan ekstrak n-heksana dengan kromatografi kolom menghasilkan empat fraksi dengan fraksi B memiliki toksisitas tertinggi sebesar 44.254 ppm. ABSTRACT: Seaweed has a role as the stability guard of the marine ecosystem, living place, and sanctuary for others and has economical potential as health materials. Several species of seaweed can be used for health cause it is contained chemicals that have biological activity. Eucheuma spinosum contains secondary metabolites such as flavonoid, triterpenoid, and steroid. This research aim is to find out more about active compounds that can be found in E. spinosum. The toxicity is determined using Brine Shrimp Lethality Test (BSLT) and the compound is identified using phytochemical screening. Extraction of 6 kg of fresh E. spinosum was using maceration method generated 12.78 g of crude extract. The crude extract is partitioned generated 5.84 g of n-hexane extract, 0.54 g of chloroform extract dan 0.52 g of n-butanol extract which consecutively showed LC50 75.199; 115.577; and 549.499 ppm. The n-hexane extract showed contains alkaloid and steroid. The n-hexane extract is separated by chromatography column generated 4 fractions which are fraction B has the highest toxicity with LC50 44.254 ppm.
AKTIVITAS ANTIBAKTERI EKSTRAK METANOL KULIT PISANG PECAH SERIBU (Musa x paradisiaca L.) TERHADAP BAKTERI Staphylococcus aureus dan Escherichia coli Wiwik Susanah Rita; Irvansyah Habibi Resaputra; I Made Sukadana
CAKRA KIMIA (Indonesian E-Journal of Applied Chemistry) Vol 8 No 2 (2020): Volume 8, Nomor 2, 2020
Publisher : Magister Program of Applied Chemistry, Udayana University, Bali-INDONESIA

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Abstract

ABSTRAK: Musa x paradisiaca L. merupakan salah satu jenis pisang yang berpotensi sebagai antibakteri. Tujuan penelitian ini adalah untuk mengetahui aktivitas antibakteri ekstrak metanol kulit buah pisang pecah seribu (Musa x paradisiaca L.) terhadap bakteri Staphylococcus aureus dan Escherichia coli serta menentukan kandungan metabolit sekundernya. Ekstraksi dilakukan dengan cara maserasi menggunakan pelarut metanol 95% pengujian aktivitas antibakteri dilakukan dengan metode sumur difusi, dan kandungan metabolit sekuder dalam ekstrak metanol dilakukan dengan uji fitokimia. Hasil uji fitokimia menunjukkan bahwa ekstrak mengandung metabolit sekunder flavonoid, fenol, alkaloid, dan triterpenoid. Hasil uji antibakteri menunjukkan bahwa Konsentrasi Hambat Minimum (KHM) dan Konsentrasi Bunuh Minimum (KBM) ekstrak metanol terhadap Staphylococcus aureus sebesar 4% dan Escherichia coli sebesar 6% dengan daya hambat masing-masing sebesar 6,83 dan 5,25 mm. ABSTRACT: Musa x paradisiaca L. is one type of banana that has potential as an antibacterial. The purpose of this study was to determine the antibacterial activity of the methanol extract of pecah seribu banana peels (Musa x paradisiaca L.) against Staphylococcus aureus and Escherichia coli and determine the content of secondary metabolites. Extraction was carried out by maceration using 95% methanol. Antibacterial activity assay was perfomed by the diffusion well method and the content of secondary metabolites in the methanol extract was carried out by phytochemical tests. The phytochemical test results show that the extract contains secondary metabolites of flavonoid, phenol, alkaloid, and triterpenoid. The antibacterial assay results revealed that the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of methanol extract against Staphylococcus aureus of 4% and Escherichia coli of 6% with inhibition of 6.83 and 5.25 mm, respectively.
POTENSI ANTIIMPLAMASI SECARA IN VIVO EKSTRAK ETANOL BATANG ANTAWALI (Tinospora sinensis) PADA TIKUS WISTAR YANG DIINDUKSI KARAGENAN Ida Ayu Putu Sri Adnyasari; Ni Made Puspawati; I Made Sukadana
CAKRA KIMIA (Indonesian E-Journal of Applied Chemistry) Vol 5 No 2 (2017)
Publisher : Magister Program of Applied Chemistry, Udayana University, Bali-INDONESIA

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Abstract

ABSTRAK: Penelitian ini bertujuan untuk menguji aktivitas antiinfamasi dari ekstrak etanol batang antawali (Tinospora sinensis) dan mengidentifikasi kandungan kimianya. Uji aktivitas antiinflamasi dilakukan secara in vivo dengan metode induksi karagenan, sedangkan identifikasi kandungan kimia secara kualiatif dengan uji fitokimia. Penelitian ini menggunakan 25 ekor tikus putih jantan galur Wistar yang dibagi menjadi 5 kelompok yaitu kontrol negatif (P0), kontrol positif (P1), dan kelompok ekstrak uji (P2, P3, dan P4) dengan dosis 125 mg/kg BB, 250 mg/kg BB, dan 500 mg/kg BB. Hasil uji aktivitas antiinflamasi menunjukkan bahwa semakin rendah dosis yang diberikan, maka akan menghasilkan persentase hambatan inflamasi yang semakin besar. Pemberian dosis 125 mg/kg BB mampu menghasilkan persentase hambatan tertinggi, diikuti dengan dosis 250 mg/kg BB, serta 500 mg/kg BB yaitu masing-masing sebesar 64,71%; 52,94%; dan 29,41% pada menit ke-360. Ekstrak etanol batang antawali memiliki nilai ED50 sebesar 51,521 mg/kg BB. Secara statistik dengan uji One Way ANOVA menunjukkan nilai p<0,05. Hasil uji fitokimia menunjukan bahwa ekstrak etanol batang Tinospora sinensis mengandung senyawa alkaloid, fenolik, dan steroid. ABSTRACT: This study aimed to evaluate the antiinflammatory activity of ethanolic extract of antawali stem (Tinospora sinensis) and to identify its phytochemical constituents. In vivo antiinflammatory activity test was performed on Wistar rat oedem induced by carrageenan, while identification of chemical constituents was done with phytochemical screening. In this study, 25 male Wistar strains were divided into 5 groups : negative control (P0), positive control (P1), test extract group (P2, P3, and P4) with doses of 125 mg/kg BW, 250 mg/kg BW, and 500 mg/kg BW. Antiinflammatory activity test results showed that the lower the dose given, it will show a greater percentage of inflammatory inhibition. The dose of 125 mg/kg BW was able to produce the highest percentage of inhibition, followed by doses of 250 mg/kg BW, and 500 mg/kg BW at 64.71%; 52.94%; and 29.41% in the 360th minute. The extract of ethanol stem antawali active as antiinflamasi with ED50 that was equal to 51,521 mg/kg BB. Statistic One Way ANOVA test showed p value < 0,05. The result of phytochemical screening showed that stem ethanolic extract of Tinospora sinensis contains alkaloids, phenolic, and steroids.
POTENSI ANTIMAKAN EKSTRAK DAUN TENGGULUN (Protium javanicum Burm. F.) PADA ULAT KUBIS (Plutella xylostella) Sri Rahayu Santi; I Made Sukadana
CAKRA KIMIA (Indonesian E-Journal of Applied Chemistry) Vol 7 No 2 (2019): volume 7, Nomor 2, 2019
Publisher : Magister Program of Applied Chemistry, Udayana University, Bali-INDONESIA

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Abstract

ABSTRAK: Daun tenggulun (Protium javanicum Burm. F.) memiliki aktivitas antimakan terhadap larva Epilachna sparsa dan berpotensi untuk dikembangkan sebagai pestisida nabati pada hama target spesifik yaitu ulat kubis (Plutella xylostella). Penelitian ini bertujuan untuk menentukan komponen senyawa yang terkandung dalam ekstrak aktif antimakan daun tenggulun terhadap ulat kubis. Hasil ekstraksi 1000 g serbuk kering daun tenggulun dengan methanol, n heksana dan etil asetat berturut-turut menghasilkan 7,2535 g ekstrak kental n-heksana , 5,5680 g ekstrak kental etil asetat, dan 20,1852 g ekstrak air. Hasil uji aktivitas antimakan menunjukkan etil setat mempunyai aktivitas terbesar ( 84,24 % pada konsentrasi 0,1%). Pemurnian ekstrak aktif etil asetat dengan kromatografi kolom ( silika gel dan fase gerak etil asetat-n-heksana (1:1)) menghasilkan tiga fraksi, dan fraksi B menunjukkan aktivitas antimakan paling tinggi (87,26% pada konsentrasi 100 ppm). Hasil analisis fraksi B dengan GC-MS menghasilkan 77 senyawa, enam diantaranya yaitu senyawa dengan Rt: 22,11 (Mr 170), 23,65’ (Mr 168), 24,6’ (Mr 220), 27,64 (Mr. 260), 31,74’ (Mr. 204), dan 32,8’ (Mr 222) teridentifikasi melalui pendekatan database NIST dan WILET 229 diduga berturut-turut adalah senyawa: linalol oksida, S(+)-5-(1-hidroksi-1-metil)-2-metil-2-sikloheksenon, 2,6-di-t-butil 2,5-sikoheksadiena 1,4-dion, karyopillen, 2-sikloheksen-1-on-4-hidroksi-3,5,6-trimetil-4-(3-oxo-butenil), dan Etil- Oktadekanoat. Kata kunci: Protium javanicum Burm. F., tenggulun, antimakan, Plutella xylostella ABSTRACT: Tenggulun leaves ((Protium javanicum Burm. F.) has antifeeding activity on Epilachna sparsa larvae and potential to be developed as pesticide on specific target such as cabbage caterpillar (Plutella xylostella). The purpose of this research is to determine chemical compounds containing on the active extracts of the leaves. The extraction process was conducted by maceration of 1000 grams the leaves with methanol followed by partition with n-hexane, ethyl acetate, and water resutling viscous extracts of 7.2535 g, 5.5680 g, and 20.1852 g respectively. Antifeeding test showed that ethyl acetate extract has the highest activity as antifeeding which is 84.24% with concentration of 0.1 %. Purification process of the ethyl acetate active extract was conducted by coloum chromatography using silica gel and mobile phase of the mixture of ethyl acetate and n-hexane (1 : 1) resulting three fraction where the B fraction showing the highest antifeeding activity which 87.26 % using solution of 100 ppm. Further, analysis the B fraction with GC-MS showed 77 chemical compounds where 6 of them identified by NIST and WILET 229 databases were confirmed as linanol oxide, S(+)5-(1-hydroxy-1-methyl)-2-methyl-2-cyclohexanone, 2,6-di-t-buthyl 2,5-cyclohexadiene 1,4-dion, caryopillen, 2-cyclohexen-1-on-4-hydroxy-3,5,6-trimethyl-4-(3-oxo-buthenyl), and ethyl octadecanoate.
KANDUNGAN SENYAWA STEROID-ALKALOID PADA EKSTRAK n-HEKSANA DAUN BERINGIN (Ficus benjamina L) I Made Sukadana
Jurnal Kimia (Journal of Chemistry) Vol. 5, No. 2 Juli 2011
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

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Abstract

Isolation of steroid-alkaloid compounds from Banyan leaf (Ficus benjamina L.) has been carried out. As much as 27.13 g of concentrated ethanol extract was resulted from 1.0 kg dried powders of Banyan leaf that was macerated using ethanol as a solvent. Further, this extract was partitioned with n-hexane to obtain 11.39 g concentrated extract of n-hexane. Ethanol extract was dissolved in ethanol-water (7:3), then was evaporated leaving water extract. Futhermore these water extract was partitioned with chloroform to obtain 0.7 g concentrated chloroform extract which was dark green and 2.21 g concentrated water extract which was green.Separations of n-hexane extract containing steroids using column chromatography (stationary phase : silica gel 60 and mobile phase : chloroform-ethylacetate (4:1)) resulted in fourteen fractions with F8 fraction being the most positive toward Liebermann-Burchard reagent, but this fraction contains at least in five components. Purification of the F8 fraction using TLC preparative resulted in six fractions (F8.1, F8.2, F8.3, F8.4, and F8.5) with F8.1 fraction being the most active steroid.The infrared spectrophotometer analysis suggested that the isolate (F8.1 fraction) was a steroid-alkaloid compound having functional groups including O-H stretching at 3448.72 cm-1 that was supported by one band C-OH streching at 1381.03 cm-1, the band of C-H aldehyde group at 2924.09 and 2854.65 cm-1 and the carbonyl group C=O of aldehyde at 1735.9 cm-1. Absorption at 2337.72 cm-1 indicated C?N stretching which was supported by N-H aliphatic and C-N aliphatic groups at 1172.72 and 1111.00 cm-1 respectively. The other groups were C=C aliphatic (1558.48 cm-1), C-H aliphatic bending (1458.18 cm-1), and C-O-C stretching (1257.59 cm-1). The gas chromatogram and mass spectra showed an isomer compound with Mr of 393 and molecular formula of C24H27O4N which was an alkaloide.
SENYAWA ANTIBAKTERI GOLONGAN FLAVONOID DARI BUAH BELIMBING MANIS (Averrhoa carambola Linn.L) I M. Sukadana
Jurnal Kimia (Journal of Chemistry) Vol. 3, No. 2 Juli 2009
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

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Abstract

Isolation and identification of active antibacterial compounds from star fruit (Averrhoa carambola Linn). hasbeen carried out. As much as 140.56 g of concentrated methanol extract was resulted from 10 kg star fruit that wasmacerated using methanol. This extract was dissolved into methanol-water (7:3) and then partitioned with n-hexaneand chloroform respectively, resulting in concentrated extracts of 0.10 g of n-hexane, 0.07 g of chloroform, and48.01 g of water. The result of phytochemical test for flavonoid for all of the extracts suggested that water extractsshowed most be test intense to the flavonoid.Separation of water extract using column chromatography resulted in FB fractions that was orange as muchas 0.2027 g and contains flavonoid. Infra red and UV-vis spectroscopy were employed in order to identify the FBfraction. The result of identification showed that isolate (fraction FB) was cathechin compounds which containedhydroxyl groups at C-3, C-7 and C-4’. It also had functional groups like –OH, C-H aromatic, C-H aliphatic, C=Caromatic, C-O alcohol, and it did not contained C=O carbonyl group. The isolate inhibited E.coli growth at 100 ppmand S. aureus grouth at 500 ppm.
ISOLASI DAN IDENTIFIKASI SENYAWA ANTIMAKAN DARI BATANG TUMBUHAN BROTOWALI (Tinospora tuberculata BEUMEE.) I M. Sukadana; Wiwik Susanah Rita; Frida R. Koreh
Jurnal Kimia (Journal of Chemistry) Vol. 1, No. 2 Juli 2007
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

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Abstract

Isolation and identification of antifeedant compounds from brotowali stem (Tinospora tuberculataBEUMEE.) was done. Brotowali dry powder of 1 kg was extracted using methanol by maceration, The methanolextract then was fractionated repeatedly by n-hexane, so that methanol and n-hexane extracts were obtained. Both ofextracts were evaporated by rotary vacuum evaporator, so concentrated methanol and n-hexane extracts wereobtained and then their antifeedant activity was tested. More active extract was separated by thin layerchromatography (TLC) then continued by column chromatography utilizes silica gel 60 as a stationary phase and thebest mobile phases of TLC. Fractions obtained were tested of their antifeedant activity. More active fraction thenwas tested its purity and identified by phytochemical test and UV-vis and infrared spectrophotometer.As much as 97.07 g of concentrated methanol extract was resulted from 1 kg brotowali dry powder.Fractionation of the methanol extract using n-hexane resulted 22.92 g of concentrated methanol and 21.71 g ofconcentrated n-hexane. n-hexane extract points out more activity as antifeedant than methanol extract. The bestmobile phase of TLC was n-hexane: chloroform (1:1 ). The unite result from the column chromatography wasobtained 3 fractions where fraction c points out antifeedant active. The purity test of fraction c was obtained 1 spotso continued by phytochemical test and UV-vis and infrared spectrophotometer. Phytochemical test andspectrophotometry analysis from active isolate (fraction c) point out that the compound included triterpenoid groupthat absorb on wavelength 288.6 nm and 310.6 nm, and be considered having functional group of O-H bonded, CO,C-H, C=O, C=C, and C-H.
AKTIVITAS ANTIBAKTERI SENYAWA GOLONGAN TRITERPENOID DARI BIJI PEPAYA (Carica papaya L.) I M. Sukadana; Sri Rahayu Santi; N. K. Juliarti
Jurnal Kimia (Journal of Chemistry) Vol. 2, No. 1 Januari 2008
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

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Abstract

Isolation, identification, and antibacterial activity examination have been conducted for triterpenoidscompounds of carica papaya L. Maseration of 500 g pawpaw seed powder in n-hexane gave 21.66 g extract whichreacts with Liebermann-Buched reagent resulting in colour change (yellow to purple). Column chromatographyseparation of the extract resulted in 0.05 g yellow isolate (F3) which contains aldehyde triterpenoids. Infrared spectraindicated aliphatic –C-H stretching, -CH2 bending, -CH3 bending, and C=O. Ultra violet – visible spectra showedabsorption at 228.5 nm and proad peak at 287.7 nm. Antibacterial axamination showed that 1000 ppm at the isolateinhibits the growth of Escherichia coli and Staphylococcus auerus.
Co-Authors A. A. Bawa Putra Ali Djamhuri Anak Agung Istri Sri Wiadnyani dan Luh Putu Arisanti Frida R. Koreh G. A. G. Indukirana I Made Bakta I Made Dira Swantara I Made Jawi I Made Oka Adi Parwata I Made Siaka I Wayan Putu Sutirta Yasa Ida Ayu Putu Sri Adnyasari Irvansyah Habibi Resaputra Karna Wijaya Melisa Anggraini Melli Melli Monikayani, Ni Wayan N. K. Juliarti N. W. Bogoriani N. W. I. Paramitha Ni Made Puspawati Putra Manuaba, I. B S. R. Santi S. Rahayu Sri Rahayu Santi Sri Wahjuni WIWIK SUSANAH RITA