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Journal : Bioscientist : Jurnal Ilmiah Biologi

Ekstrak Daun Jati (Tectona grandis) Alternatif Pewarna pada Penghitungan Jumlah dan Viabilitas Sel Kultur Dibandingkan dengan Pewarna Tryphan Blue Sri Andayani, I Gusti Ayu; Sulastri, Sri; Hananto, Dwi Ampera; Sriasih, Made
Bioscientist : Jurnal Ilmiah Biologi Vol 8, No 2 (2020): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bjib.v8i2.3015

Abstract

Tryphan blue comes from toluidine which is one of the bases of toluene isomer which has a natural pigment content of anthsiasin. Tryphan blue is commonly used for cell counting and for the calculation of tissue viability in laboratory mice. Basically the use of tryphan blue is dangerous for laboratory workers because it has a carcinogenic effect, mutagenesis, so other materials are needed especially safer and harmless natural materials. This study aims to compare the quality of teak leaf dye extract as a natural dye on the calculation of the number and viability of culture cells compared to tryphan blue dye. The study used the Complete Randomized Design (RAL) method with three treatment factors (96% ethanol, 14% citric acid, and Phospat Buffer Saline (PBS)) and observations of 5 replays be used for the calculation of the number and viability of spleen cells in the 1st, 10th, 20th, and 30th minutes with tryphan blue as a positive control. The results showed that the quality of teak leaf dye with ethanol solvent 96% viability does not differ materially, provides good color quality and obtained the same viability time as tryphan blue.
Co-Authors A. H. Sutanto, A. H. Abdullah, Uhud Achmad Taufik Adi Gunawan, Adi Adrian Gunawan, Adrian Agus Sukarno Alamsyah, Rifan Aldy Rialdy Atmadja, Aldy Rialdy Anisa Zairina Arliani, Pradini Nur’amalia Ashadi Sasongko Atan Baas Sinuhaji, Atan Baas BASAR, GIGIN G. KAMIL BASAR, GIGIN G. KAMIL Baskoro Adi Prayitno Budi M. Taftazani Budiman Tampubolon Chairul Yoel, Chairul Chichik Nirmasar, Chichik Chika Nur Pebriani, Chika Nur Danny Dwi Septianto, Danny Dwi Dedeh Dedeh, Dedeh Dessy Hasanah Siti Asiah, Dessy Hasanah Siti Devi Ayu Rizki, Devi Ayu Dwi Kusuma Purnamasari, Dwi Kusuma Erwan Erwan, Erwan Fifi Puspita Gigin Ginanjar Kamil Basar, Gigin Ginanjar Kamil Hadi, Sulvana Hananto, Dwi Ampera Harahap, Chairul Adillah Harahap, Sari Leyli Hery Wibowo, Hery Hetty Krisnani, Hetty I Made Sudana Jaya, Asrul Johnwan Usman, Johnwan Kodaruddin, Wina Nurdini Krisrupianti, Lupi Kurnia Sholihah, Kurnia Lusi Epsilawati Lusianai, Wa Ode M. Iqbal Made Sriasih, Made Majidah Maridi Maridi Masrul Masrul Maulana Irfan, Maulana Meilanny Budiarti S., Meilanny Budiarti MUHAMMAD ALI Muhammad Ferdryansyah, Muhammad Nadir Naim, Muh Rizman Nandang Mulyana, Nandang Ngurah Intan Wiratmini NI LUH SURIANI Noersida Raid, Noersida Nur Hidayat NURHAQIM, SONI AKHMAD NURHAQIM, SONI AKHMAD Pakendek, Adriana Pardi Pardi, Pardi Pradini Nur’amalia Arliani, Pradini Nur’amalia Purwanto, Moch RAMADHANI, WIDYA SUCI RAMADHANI, WIDYA SUCI Rendy H Pratama, Rendy H Ria Noerianingsih Firman, Ria Noerianingsih Rindang Ekawati Roko Patria Jati, Roko Patria Rudi Saprudin Darwis, Rudi Saprudin Sahadi Humaedi Sari, Diah Puspita Sigit Widiyarto, Sigit Sopyan, Taupik Sri Andayani, I Gusti Ayu Sumiati Sumiati Suryani . Sutresna, Yoyon Suwirta, Utty Syamsuhaidi, Syamsuhaidi Triana, Annisya Usman, Lismayani Utami Dyah Syafitri Wardani, Win Yuli Widya Hary Cahyati