Suliasih Suliasih
Pusat Penelitian Biologi, Lembaga Ilmu Pengetahuan Indonesia, Jln. Raya Jakarta-Bogor Km. 46, Cibinong Science Center, Jawa Barat, Indonesia

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PENGUJIAN PUPUK HAYATI KALBAR UNTUK MENINGKATKAN PRODUKTIVITAS TANAMAN KEDELAI (Glycine max) var. BALURAN Subowo, YB.; Sugiharto, Arwan; Suliasih, Suliasih; Widawati, Sri
Caraka Tani - Jurnal Ilmu Ilmu Pertanian Vol 25, No 1 (2010)
Publisher : Caraka Tani - Jurnal Ilmu Ilmu Pertanian

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Penelitian     mengarah     pada     evaluasi     kemampuan     dari      pupuk     hayati     kalbar     untuk meningkatkan   produktivitas  dari  tanaman  kedelai.  Percobaan  pada  plot  percobaan  lapangan  di  LIPI Cibinong.  Kacang  kedelai(  Glycine  max )   variasi  Baluran  diperlakukan  dengan  satu rangkaian  pupuk yaitu: Nitrogen berisi kompos mikroba perbaikan, Fosfat berisi kompos mikroba solubilizing, kompos mengandung lignocelullosic menurunkan populasi jamur, Pupuk hayati Kalbar (Kompos memperbaiki kandungan  nitrogem, Fosfat solubilizing  dan lignocellulosic  mengurangi  derajat mikroba), pupuk kimia, kompos  dan  kontrol.  Kenaikan  pupuk    berat  segar  dari  biomass,  angka  dari  daun-daun,  angka  buah polong, dan berat dari biji diamati. Nitrogen, Fosfat dan Karbon yang berada pada tanah sebelum tanaman, saat   fase  generatif   pembungaan   dan   saat   panen.   Hasil   aplikasi   pengujian   pupuk   kalbar   mampu meningkatkan  Karbon  (C  ), Nitrogen  (N  ) dan  Phosphor  (P  ) konten  pada  tanah.  Perlakuan  ini  juga meningkatkan berat segar dari biomass (22%), buah polong (11, 11%) dan berat dari biji (12,22%).
Pengaruh Kompos yang Diperkaya Bakteri Penambat Nitrogen dan Pelarut Fosfat terhadap Pertumbuhan Tanaman Kapri dan Aktivitas Enzim Fosfatase dalam Tanah Widawati, S; Suliasih, Suliasih; Muharam, Agus
Jurnal Hortikultura Vol 20, No 3 (2010): September 2010
Publisher : Indonesian Center for Horticultural Research and Development

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ABSTRAK. Penggunaan pupuk organik dalam budidaya sayuran memiliki beberapa keuntungan, terutama untukmempertahankan kondisi tanah dan menekan penggunaan pupuk anorganik. Penelitian dilaksanakan di DesaCidawu (1.250 m dpl.), Cibodas, Kabupaten Cianjur, Provinsi Jawa Barat, mulai bulan Januari sampai Desember2007. Sampel tanah dikoleksi dari daerah berbeda di Pontianak, Kalimantan Barat. Penelitian bertujuan mengetahuipengaruh kompos yang diperkaya dengan bakteri pelarut fosfat (phosphate solubilizing bacteria=PSB) terhadappertumbuhan tanaman kapri dan aktivitas enzim fosfatase di dalam tanah. Penelitian menggunakan rancangan acakkelompok dengan lima perlakuan dan tiga ulangan. Varietas kapri yang digunakan ialah varietas lokal. Perlakuanterdiri atas (A) tanpa pupuk, (B) pupuk anorganik (TSP+KCl+Urea), (C) kotoran ayam + sekam, (D) kompos, dan(E) kompos plus, yaitu kompos yang diberi campuran bakteri, yaitu bakteri penambat nitrogen simbiotik, bakteripenambat nitrogen nonsimbiotik, dan bakteri pelarut fosfat. Hasil penelitian menunjukkan bahwa campuran bakteriyang diisolasi dari tanah gambut di Kalimantan Barat yang terkandung dalam kompos plus dapat beradaptasi denganbaik pada lahan di lokasi penelitian. Populasi total SNFB, NSNFB, dan PSB meningkat masing-masing menjadi 9,15x 108, 9,34 x 108, dan 9,35 x 108 sel/g tanah. Keberadaan campuran bakteri tersebut mampu meningkatkan aktivitasenzim fosfatase asam dan basa di dalam tanah. Peningkatan tertinggi aktivitas enzim fosfomonoesterase asam danbasa dicapai pada perlakuan kompos plus. Perlakuan tersebut juga meningkatkan berat buah kapri dibandingkandengan kontrol (75,32 %), perlakuan pupuk kimia (45,48%), kotoran ayam + sekam ( 31,19 %), dan kompos (15,60%). Pemanfaatan campuran bakteri pelarut fosfat tersebut dalam kompos diharapkan dapat digunakan secara meluasdalam pembudidayaan kapri dalam sistem organic farming (OF), sehingga berperan dalam peningkatan produksi dansekaligus menekan penggunaan pupuk anorganik.ABSTRACT. Widawati, S., Suliasih, and A. Muharam. 2010. The Effect of Compost Enriched with SymbioticNitrogen Fixing and Phosphate Solubilizing Bacteria on the Growth of Peas and the Activity of PhosphataseEnzymes in the Soil. The use of organic materials on cultivation of vegetable crops has some advantages, especiallyfor maintaining suitable soil conditions and decreasing the utilization of inorganic fertilizers. The research wascarried out at Cidawu Village, Cibodas (1,250 m asl.), Cianjur, West Java, from January to December 2007. Soilsamples were collected from some different areas in Pontianak, West Kalimantan. The research was aimed todetermine the effect of compost enriched with mixed phosphate solubilizing bacteria (PSB) on the growth of peasand on the activity of phosphatase enzymes in the soil. A randomized block design with five treatments and threereplications was used in the experiment. A local variety of peas was utilized in the experiment. The treatments werethe addition of fertilizers consisted of (A) without any fertilizer, (B) with inorganic fertilizers i.e. TSP+KCl+Urea,(C) with chicken dung + rice husk, (D) with compost, and (E) with compost plus (compost enriched with symbioticnitrogen fixing bacteria/SNFB, nonsymbiotic nitrogen fixing bacteria/NSNFB, and PSB). The results showed thatthe mixed bacteria isolated from peat soil in West Kalimantan in the compost plus (treatment E) properly adaptedin soil conditions at the site of the experiment. The total populations of SNFB, NSNFB, and PSB were increased upto 9.15 x 108, 9.34 x 108, and 9.35 x 108 cell/g soil, respectively. The occurrence of the mixed bacteria increased theactivity of acid and alkaline phosphatases in the soil. The highest activities of acid and alkaline phosphomonoesteraseenzymes in the soil achieved by the treatment of compost plus. The treatment increased the fresh weight of peascompared to control (75.32 %), to the chemical fertilizers (45.48%), to chicken dung+rice husk ( 31.19 %), and tocompost (15.60 %). The widely application of the mixed PSB in compost is hopefully established in cultivation ofpeas in the organic farming (OF) system, then it will confidently support on the increase of peas production, and thedecrease of inorganic fertilizers as well.
Aplikasi Pupuk Organik dan Bakteri Pelarut Fosfat untuk Meningkatkan Pertumbuhan Tanaman Tomat dan Aktivitas Mikroba Tanah Suliasih, Suliasih; Widawati, S; Muharam, Agus
Jurnal Hortikultura Vol 20, No 3 (2010): September 2010
Publisher : Indonesian Center for Horticultural Research and Development

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ABSTRAK. Pemanfaatan bahan organik untuk budidaya tomat dewasa ini difokuskan untuk menekan penggunaanbahan kimia yang berlebihan, sehingga kerusakan lingkungan dapat diminimalkan. Percobaan dilaksanakan di DesaCidawu, Cibodas, Cianjur (1.250 m dpl.), sejak Januari sampai Desember 2007. Pupuk organik yang digunakanyaitu kompos dan kotoran ayam + sekam, sedangkan pupuk hayati yaitu bakteri pelarut fosfat (phosphatesolubilizing bacteria = PSB). Tujuan percobaan adalah mendapatkan inokulan yang efektif untuk meningkatkanpertumbuhan dan hasil tomat, serta menstimulasi aktivitas mikroba dan enzim fosfatase di dalam tanah. Percobaanmenggunakan rancangan acak kelompok dengan lima perlakuan, yaitu tanpa pemupukan maupun inokulan (K),pemberian inokulan PSB (P1), pemberian kompos (P2), pemberian kotoran ayam+sekam (P3), dan pemberianpupuk kimia NPK (P4), masing-masing dengan tiga kali ulangan. Varietas tomat yang digunakan adalah Gondol.Hasil percobaan menunjukkan bahwa pemberian pupuk organik atau inokulan PSB dapat meningkatkan pertumbuhantanaman tomat dan hasil buahnya, serta populasi PSB dan aktivitas enzim fosfatase di dalam tanah. Pemberianinokulan PSB menghasilkan peubah tinggi tanaman tomat tertinggi (108,3 cm) dibandingkan dengan kontrol (72,3cm), dan meningkatkan hasil buah sebanyak 88,2% dibandingkan dengan kontrol, dan juga lebih tinggi daripadapemberian pupuk kimia NPK. Peningkatan tertinggi populasi PSB dan aktivitas enzim fosfatase asam serta basadalam tanah setelah panen tomat juga diperoleh dari pemberian inokulan PSB dibandingkan dengan perlakuanlain. Implementasi inokulan PSB dan pupuk organik secara meluas dalam pembudidayaan tomat diharapkandapat mendorong peningkatan produksi, produktivitas, dan mutu buah tomat untuk memenuhi kebutuhan pasar.ABSTRACT. Suliasih, S. Widawati, and A. Muharam. 2010. The Application of Organic Fertilizers andPhosphate Solubilizing Bacteria to Increase the Growth of Tomato and Soil Microbial Activities. The use oforganic matters on tomato cultivation is focused to decrease the utilization of chemical substances for minimizingenvironmental degradation. An experiment was conducted at Cidawu Village, Cibodas, Cianjur (1,250 m asl.) todetermine the suitable and effective inoculant to increase the growth of tomato plants, and also to stimulate soil microbialactivities. The tomato variety used was Gondol. The organic fertilizers were compost, chicken dung plus rice husk,and phosphate solubilizing bacteria (PSB), as a biofertilizer. A randomized block design with three replications wasutilized in this experiment. The treatments consisted of without organic fertilizers or PSB (control) (K), inoculationof PSB (P1), application of compost (P2), (4) application of chicken dung plus rice husk (P3), and application of thechemical fertilizer (NPK) (P4). The results showed that the application of organic fertilizers and PSB increased thegrowth of tomato plants and also PSB population, acid, and alkaline phosphatase activities in the soil after harvesting.The inoculation of PSB resulted in heighest plant height (108.3 cm) compared to control (72.3 cm) and also thehighest tomato yield compared to the control treatment (88.2%), and even it was higher than the yield caused by theapplication of the chemical fertilizer. The highest increase of PSB population and the activities of acid and alkalinephosphatase enzymes in soil after harvesting was also caused by the application of the PSB inoculant compared to theother treatments. The applications of organic fertilizers and PSB for wide scale cultivation of tomato expectantly playan important role for increasing production, productivity, and quality of tomato to fulfill market demand for the product.
Pengaruh Introduksi Kompos Plus terhadap Produkasi Bobot Kering Daun Kumis Kucing (Orthosiphon aristatus) pada Tiga Macam Media Tanah Widawati, Sri; Suliasih, Suliasih; Syaifudin, Syaifudin
JURNAL BIOLOGI INDONESIA Vol 3, No 3 (2002): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (207.723 KB) | DOI: 10.14203/jbi.v3i3.3475

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ABSTRACTA green house experiment were conducted to study the application of “compost plus” on the growth of Orthosiphon aristatus. The experiment was designed in Complete Randomized Design with factorial and five replicates. The first factors were fertilizer application (compost plus, compost, and control). The second factors were 3 kinds of soil (i.e. soil from Cibinong, Ciomas, and Sukabumi). The result showed that compost plus application increased 113.90 gram/pot of dry weight of Orthosispon aristatus leaves compared with compost application and control in 3 kinds of soil.Key words : Compost plus (compost + microbes), Orthosispon aristatus
Daya Pacu Mikroba Pelarut Fosfat dan Penambat Nitrogen pada Tanaman Jagung Latupapua, H.J.D.; Suliasih, Suliasih
JURNAL BIOLOGI INDONESIA Vol 3, No 2 (2001): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (199.511 KB) | DOI: 10.14203/jbi.v3i2.3477

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ABSTRACTThe Stimulating Capacities of the Phosphate Solubilizing and the Nitrogen Fixing Microbes on the Maize Plant. The green house experiment has been carried out to study the effectiveness of the phosphate solubilizing microbes (PSM) and nitrogen fixing bacteria (NFB) on the growth of maize (Zea mays). The phosphate solubilizing microbes were Aspergillus sp. and Gigaspora margarita while the nitrogen fixing bacteria were Azospirillum sp. and Azotobacter sp. have been used in this experiment. The aim of the experiment was to find the suitable and effective inoculant on the maize and its expected the growth of the plant. The experiment used The Completely Randomize Design with three replicates. The treatments were uninoculated plant (control), the plants inoculated with single microbe of PSM, NFB and the combination of both. The results indicated that the phosphate solubilizing microbes (PSM) and the nitrogen fixing bacteria (NFB) which were either used in single or combination formula could stimulate the plant growth. The dual inoculation of Azotobacter sp. and Aspergillus sp. were found the higest yield of maize.Key words : The phosphate solubilizing microbes, the nitrogen fixing bacteria, Mycorrhiza vesicular arbuscular, Zea mays.
KARAKTER RESPIRASI DAN MINERALISASI KARBON ORGANIK PADA SAMPEL TANAH DIKOLEKSI DARI PULAU BANGKA [Respiration and Organic Carbon Mineralization Character in Soil Samples Collected from Bangka Island] Rahmansyah, Maman; Suliasih, Suliasih
BERITA BIOLOGI Vol 15, No 1 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3877.84 KB) | DOI: 10.14203/beritabiologi.v15i1.2855

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The study was designed to explore soil biomass content and soil enzymatic activities that involved in carbon organic soil mineralization. Samples of soil were collected from two locations in Bangka Island. Bulk samples of top soil (TP) and excavated (TG)  were collected from garden soil in Pangkalpinang. Other soils were gathered from forest floor (TU), beneath pelawan (Tristaniopsis merguensis Griff.) trees which is endemic plant to North Bangka forest. Soil biological character was evaluated by measuring soil microbial population, respiration rate, and cellulase and amylase activities. Experiments were carried out in Research Center for Biology, Indonesian Institute of Sciences, on dried soil treatment (Control), soil moistened with 5 mL H2O in 100g soil (Gluc.0), and soil moistened with 5 mL 1% glucose solution (Gluc.1). Bacterial population obtained was higher than fungal population in all soil samples. Soil respiration activity in TU after treatment was 4.51±0.49; 5.26±0,79; and 8.28±1.41 ppm CO2/100g-soil/5-minutes, respectively. Meanwhile , respiration in TP  were 3.65 ± 2.12; 3.57 ± 1.18; and 7.94 ± 1.05 ppm CO2/100g-soil/5-minutes; and in the TG are 2.61 ± 0.70; 3.34 ± 0.94; and 5.46 ± 2.30 ppm CO2/100gsoil/5-minutes. Cellulase activity of all samples were not significantly different. Forest soil cellulase activity was significantly different compared to garden soil. Glucose induction was positively increased amylase activity compared to cellulase. Soil enzyme, soil respiration activities and microbial population of forest soil could be used as a reference to recover degraded land in Bangka Island.
PENGARUH SALINITAS DAN INOKULAN BAKTERI TERHADAP PERTUMBUHAN TANAMAN TERUNG (Solanum melongena L.) [The Effect of Salinity and Bacteria Inoculant on The Growth of Eggplant (Solanum melongena L.)] Suliasih, Suliasih; Widawati, Sri
BERITA BIOLOGI Vol 15, No 1 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3403.499 KB) | DOI: 10.14203/beritabiologi.v15i1.2854

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Nitrogen fixing dan phosphate solubilizing bacteria are important PGPR bacterial in addition of plant nutrients by increasing N and P, especially in saline soils. Experiments were conducted to observe the effects of  soil salinity and bacterial inoculant on growth eggplant (Solanum melongena) at green house of  Microbiology division, Research Center for Biology, The Indonesian Institute of Sciences,  Cibinong. Experiment laid out as factorial based randomized complete design  with three replications. Five levels of watering consisted of (1). Fresh water, (2). NaCl 0.1%, (3). NaCl 1%, (4). Sea water 75 %+25% fresh water, (5). Sea water 100%  as first factors. Five levels of inoculations/biofertilizers consisted of (1).control without fertilizer (K), (2). NPK (P), (3). Nitrogen Fixing bacteria (BPN1), all treatments was repeated 3 times  BPN2, mixed inoculation (BPN1 +  BPN2 + Phosphate Solubilizing Bacteria/BPF) as second factors.  Results showed that biofertilizer application increased growth and yield of eggplant in various levels of salinity at about 21.56%-53.35%  compared to uninoculated plants
AKTIVITAS FOSFATASE TANAH DILINGKUNGAN BENTANG HUTAN ALAMI DAN NON-ALAMI Suliasih, Suliasih; Rahmansyah, Maman
BERITA BIOLOGI Vol 9, No 6 (2009)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (656.287 KB) | DOI: 10.14203/beritabiologi.v9i6.856

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The spatial dispersal of acid and alkaline phosphates activity in forest soils appears to be controlled by position in the landscape and its soil microbial density. Soil bacteria expressively produced significant level of acid phosphatase in the investigation.The variety of acid phosphatase activity (8.25-37.55 />-nitrophenol.g"soil.h") noted higher and followed by alkaline (0.78-7.15 p-nitrophenol.g-soil.h" ), correspondingly. Acid and alkaline phosphates were exist in both of soil of natural as well as for non-natural forest soil landscape, with the value (po/o=0.6210) of correlation are 0.6889 and 0.6532, respectively. Phosphate Solubilizing Bacteria (PSB) average density in natural forest soil equivalent to 0.61xl0Â colony forming unit (cfu) and total bacteria is 160xl0 cfu, while the PSB in non-natural forest soil is 7.75xl0 cfu and total bacteria is 48.25x10 cfu. Concerning to the forest environment as soil bacteria inhabitants of PSB, there were significantly difference of PSB population in natural and non-natural landscape forest. Forest environment is negatively affect the soil enzymes activities under Pinus maritima and Caliandra calothyrsus as the lowest activities, and to the highest ones under the Schima wallichii and Eucalyptus saligna vegetation.
BIODIVERSITAS Basidiomycetes DIKECAMATAN KELILA, KABUPATEN JAYAWIJAYA, PROVINSI PAPUA DAN MANFAATNYA SEBAGAI BAHAN MAKANAN DAN OBAT TRADISIONAL Imamuddin, Hartati; Suliasih, Suliasih
BERITA BIOLOGI Vol 6, No 5 (2003)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (284.366 KB) | DOI: 10.14203/beritabiologi.v6i5.1195

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Biodiversity of Basidiomycetes was studied in Kelila, Jayawijaya, Papua.The aim of this observation was to identify species diversity of Basidiomycetes and their utilities. Sixty one species were found in the location. The 19 species were edible mushroom while 7 species have been used as traditional medicines and some species have a common odours (smell) and 1 species (Clavatia sp.) contains clavatin which could be used as arm tumour agent.
THE POPULATION OF NITROGEN FIXING BACTERIA AND PHOSPHATE SOLUBILIZING BACTERIA IN THE RHIZOSPHERE FROM GUNUNG HALIMUN NATIONAL PARK Widawati, Sri; Suliasih, Suliasih
BERITA BIOLOGI Vol 5, No 6 (2001)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (266.562 KB) | DOI: 10.14203/beritabiologi.v5i6.1075

Abstract

The biodiversity of nitrogen fixing bacteria (Rhizobium, Azotobacter and Azospirillum) and phosphate solubilizing bacteria in rhizosphere collected from Gunung Halimun National Park were studied.Bacterial population was counted from soil rhizosphere collected from various ecosystem types (determined based on elevation) namely 600 m, 1000 m, 1100 m, 1500 m and 1800 m above sea level.The results showed that the highest population of N-fixing and P solubilizing bacteria at Cikaniki (1100 asl) are founded in the plant rhizosphere of Schima wallichii in plot Suzuki A I and Altingia exelsa in Suzuki A III,at Gunung Botol area is in 1000 m asl,and Ciptarasa area is in 600 m asl.The population of N-fixing and P solubilizing bacteria at Gunung Halimun was influenced by the vegetation type, soil pH,and the elevation of area. Rhizobium, Azotobacter, Azospirillum (N-fixing bacteria); Pseudomonas sp, and Bacillus megaterium (P solubilizing bacteria), are most dominant at 600 m, 1000 m and 1100 m asl.