Suliasih Suliasih
Pusat Penelitian Biologi, Lembaga Ilmu Pengetahuan Indonesia, Jln. Raya Jakarta-Bogor Km. 46, Cibinong Science Center, Jawa Barat, Indonesia

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KEBERADAAN BAKTERI PELARUT FOSFAT DAN AKTIVITAS ENZIM FOSFATASE TANAH DAERAH PERAKARAN TANAMAN OBAT DARI KEBUN RAYA CIBODAS suliasih, Suliasih
Jurnal Rekayasa Lingkungan Vol 6, No 3: Jurnal Rekayasa Lingkungan
Publisher : BPPT

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (112.563 KB)

Abstract

A study was undertaken to investigate to occurance of phosphate solubilizing bacteria from rhizosphere soil samples of medicine plants in Cibodas Botanical Garden. 13 soil samples of medicine plants are collected randomly The result shows that 71 isolates of phosphate solubilizing bacteria were isolated, and 10 species of these organism was identified as Azotobacter sp, Bacillus sp, Chromobacterium sp, C.violaceum, Citrobacter sp. , Enterobacter sp., E. liquefaciens. Nitrosomonas sp., Serratia rubidaea, Sphaerotillus natans. Azotobacter sp. And Bacillus sp. Are found in all of soil tested. Conversely, Serratia rubidaea is only in the sample from rhizosphere of Plantago mayor The activity of acid alkaline phosphatase in soil tested ranged from 0.78 – 60,18 ugp nitrophenole/g/h, with the higest values being recorded in soil sample from rhizosphere of “Lavender”.Keywords : phosphate solubilizing bacteria, soil enzyme phosphatase
PENGUJIAN PUPUK HAYATI KALBAR UNTUK MENINGKATKAN PRODUKTIVITAS TANAMAN KEDELAI (Glycine max) var. BALURAN Subowo, Yohanes Bernard; Sugiharto, Arwan; Suliasih, Suliasih; Widawati, Sri
Caraka Tani: Journal of Sustainable Agriculture Vol 25, No 1 (2010): March
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (57.022 KB) | DOI: 10.20961/carakatani.v25i1.15756

Abstract

Research aimed to evaluate the ability of kalbar biofertilizer to improve productivity of soybean. Experiment was conducted in the field experimental plot of Cibinong Science Centre. Soybean (Glycine max) var Baluran was treated with a series of fertilizer i.e.: compost containing Nitrogen fixing microbes, compost containing Phosphate solubilizing microbes, compost containing lignocelullosic degrading fungus, Kalbar biofertilizer (compost containing Nitrogen fixing, Phosphate solubilizing and lignocellulosic degrading microbes), chemist fertilizer, compost and control. The height of plant, fresh weight of biomass, number of leaves, pod number, and weight of seed were observed. The Nitrogen, Phosphate and Carbon which exist on the soil observed before planting, while flowering and while harvesting. The result was the application of kalbar biofertilizer able to increase the Carbon (C), Nitrogen (N) and Phosphor (P) content in the soil. This treatment also increased the fresh weight of biomass (22%), the number of pods (11, 11%) and the weight of seed (12,22%).
PENGARUH INTRODUKSI KOMPOS PLUS TERHADAP PRODUKASI BOBOT KERING DAUN KUMIS KUCING (ORTHOSIPHON ARISTATUS) PADA TIGA MACAM MEDIA TANAH Widawati, Sri; Suliasih, Suliasih; Syaifudin, Syaifudin
JURNAL BIOLOGI INDONESIA Vol 3, No 3 (2002): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v3i3.3475

Abstract

ABSTRACTA green house experiment were conducted to study the application of ?compost plus? on the growth of Orthosiphon aristatus. The experiment was designed in Complete Randomized Design with factorial and five replicates. The first factors were fertilizer application (compost plus, compost, and control). The second factors were 3 kinds of soil (i.e. soil from Cibinong, Ciomas, and Sukabumi). The result showed that compost plus application increased 113.90 gram/pot of dry weight of Orthosispon aristatus leaves compared with compost application and control in 3 kinds of soil.Key words : Compost plus (compost + microbes), Orthosispon aristatus
DAYA PACU MIKROBA PELARUT FOSFAT DAN PENAMBAT NITROGEN PADA TANAMAN JAGUNG Latupapua, H.J.D.; Suliasih, Suliasih
JURNAL BIOLOGI INDONESIA Vol 3, No 2 (2001): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v3i2.3477

Abstract

ABSTRACTThe Stimulating Capacities of the Phosphate Solubilizing and the Nitrogen Fixing Microbes on the Maize Plant. The green house experiment has been carried out to study the effectiveness of the phosphate solubilizing microbes (PSM) and nitrogen fixing bacteria (NFB) on the growth of maize (Zea mays). The phosphate solubilizing microbes were Aspergillus sp. and Gigaspora margarita while the nitrogen fixing bacteria were Azospirillum sp. and Azotobacter sp. have been used in this experiment. The aim of the experiment was to find the suitable and effective inoculant on the maize and its expected the growth of the plant. The experiment used The Completely Randomize Design with three replicates. The treatments were uninoculated plant (control), the plants inoculated with single microbe of PSM, NFB and the combination of both. The results indicated that the phosphate solubilizing microbes (PSM) and the nitrogen fixing bacteria (NFB) which were either used in single or combination formula could stimulate the plant growth. The dual inoculation of Azotobacter sp. and Aspergillus sp. were found the higest yield of maize.Key words : The phosphate solubilizing microbes, the nitrogen fixing bacteria, Mycorrhiza vesicular arbuscular, Zea mays.
KEBERADAAN BAKTERI PELARUT FOSFAT DAN AKTIVITAS ENZIM FOSFATASE TANAH DAERAH PERAKARAN TANAMAN OBAT DARI KEBUN RAYA CIBODAS suliasih, Suliasih
Jurnal Rekayasa Lingkungan Vol 6, No 3: Jurnal Rekayasa Lingkungan
Publisher : BPPT

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (112.563 KB)

Abstract

A study was undertaken to investigate to occurance of phosphate solubilizing bacteria from rhizosphere soil samples of medicine plants in Cibodas Botanical Garden. 13 soil samples of medicine plants are collected randomly The result shows that 71 isolates of phosphate solubilizing bacteria were isolated, and 10 species of these organism was identified as Azotobacter sp, Bacillus sp, Chromobacterium sp, C.violaceum, Citrobacter sp. , Enterobacter sp., E. liquefaciens. Nitrosomonas sp., Serratia rubidaea, Sphaerotillus natans. Azotobacter sp. And Bacillus sp. Are found in all of soil tested. Conversely, Serratia rubidaea is only in the sample from rhizosphere of Plantago mayor The activity of acid alkaline phosphatase in soil tested ranged from 0.78 ? 60,18 ugp nitrophenole/g/h, with the higest values being recorded in soil sample from rhizosphere of ?Lavender?.Keywords : phosphate solubilizing bacteria, soil enzyme phosphatase
Pengaruh Kompos yang Diperkaya Bakteri Penambat Nitrogen dan Pelarut Fosfat terhadap Pertumbuhan Tanaman Kapri dan Aktivitas Enzim Fosfatase dalam Tanah Widawati, S; Suliasih, Suliasih; Muharam, Agus
Jurnal Hortikultura Vol 20, No 3 (2010): September 2010
Publisher : Indonesian Center for Horticulture Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jhort.v20n3.2010.p%p

Abstract

ABSTRAK. Penggunaan pupuk organik dalam budidaya sayuran memiliki beberapa keuntungan, terutama untukmempertahankan kondisi tanah dan menekan penggunaan pupuk anorganik. Penelitian dilaksanakan di DesaCidawu (1.250 m dpl.), Cibodas, Kabupaten Cianjur, Provinsi Jawa Barat, mulai bulan Januari sampai Desember2007. Sampel tanah dikoleksi dari daerah berbeda di Pontianak, Kalimantan Barat. Penelitian bertujuan mengetahuipengaruh kompos yang diperkaya dengan bakteri pelarut fosfat (phosphate solubilizing bacteria=PSB) terhadappertumbuhan tanaman kapri dan aktivitas enzim fosfatase di dalam tanah. Penelitian menggunakan rancangan acakkelompok dengan lima perlakuan dan tiga ulangan. Varietas kapri yang digunakan ialah varietas lokal. Perlakuanterdiri atas (A) tanpa pupuk, (B) pupuk anorganik (TSP+KCl+Urea), (C) kotoran ayam + sekam, (D) kompos, dan(E) kompos plus, yaitu kompos yang diberi campuran bakteri, yaitu bakteri penambat nitrogen simbiotik, bakteripenambat nitrogen nonsimbiotik, dan bakteri pelarut fosfat. Hasil penelitian menunjukkan bahwa campuran bakteriyang diisolasi dari tanah gambut di Kalimantan Barat yang terkandung dalam kompos plus dapat beradaptasi denganbaik pada lahan di lokasi penelitian. Populasi total SNFB, NSNFB, dan PSB meningkat masing-masing menjadi 9,15x 108, 9,34 x 108, dan 9,35 x 108 sel/g tanah. Keberadaan campuran bakteri tersebut mampu meningkatkan aktivitasenzim fosfatase asam dan basa di dalam tanah. Peningkatan tertinggi aktivitas enzim fosfomonoesterase asam danbasa dicapai pada perlakuan kompos plus. Perlakuan tersebut juga meningkatkan berat buah kapri dibandingkandengan kontrol (75,32 %), perlakuan pupuk kimia (45,48%), kotoran ayam + sekam ( 31,19 %), dan kompos (15,60%). Pemanfaatan campuran bakteri pelarut fosfat tersebut dalam kompos diharapkan dapat digunakan secara meluasdalam pembudidayaan kapri dalam sistem organic farming (OF), sehingga berperan dalam peningkatan produksi dansekaligus menekan penggunaan pupuk anorganik.ABSTRACT. Widawati, S., Suliasih, and A. Muharam. 2010. The Effect of Compost Enriched with SymbioticNitrogen Fixing and Phosphate Solubilizing Bacteria on the Growth of Peas and the Activity of PhosphataseEnzymes in the Soil. The use of organic materials on cultivation of vegetable crops has some advantages, especiallyfor maintaining suitable soil conditions and decreasing the utilization of inorganic fertilizers. The research wascarried out at Cidawu Village, Cibodas (1,250 m asl.), Cianjur, West Java, from January to December 2007. Soilsamples were collected from some different areas in Pontianak, West Kalimantan. The research was aimed todetermine the effect of compost enriched with mixed phosphate solubilizing bacteria (PSB) on the growth of peasand on the activity of phosphatase enzymes in the soil. A randomized block design with five treatments and threereplications was used in the experiment. A local variety of peas was utilized in the experiment. The treatments werethe addition of fertilizers consisted of (A) without any fertilizer, (B) with inorganic fertilizers i.e. TSP+KCl+Urea,(C) with chicken dung + rice husk, (D) with compost, and (E) with compost plus (compost enriched with symbioticnitrogen fixing bacteria/SNFB, nonsymbiotic nitrogen fixing bacteria/NSNFB, and PSB). The results showed thatthe mixed bacteria isolated from peat soil in West Kalimantan in the compost plus (treatment E) properly adaptedin soil conditions at the site of the experiment. The total populations of SNFB, NSNFB, and PSB were increased upto 9.15 x 108, 9.34 x 108, and 9.35 x 108 cell/g soil, respectively. The occurrence of the mixed bacteria increased theactivity of acid and alkaline phosphatases in the soil. The highest activities of acid and alkaline phosphomonoesteraseenzymes in the soil achieved by the treatment of compost plus. The treatment increased the fresh weight of peascompared to control (75.32 %), to the chemical fertilizers (45.48%), to chicken dung+rice husk ( 31.19 %), and tocompost (15.60 %). The widely application of the mixed PSB in compost is hopefully established in cultivation ofpeas in the organic farming (OF) system, then it will confidently support on the increase of peas production, and thedecrease of inorganic fertilizers as well.