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AKTIVITAS ENZIM 2,4-D MONOOKSIGENASE DARI BERBAGAI MIKROBA Nunik Sulistinah
BERITA BIOLOGI Vol 4, No 5 (1999)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v4i5.1242

Abstract

Nine cultures both from fungi and bacteria have been selected for testing their 2,4-D monooxygenase activity to degrade 2,4-Dtohlorophenoxyacetic acid (2,4-D). The results showed that all the cultures which were tested grows at 1000 ppm 2,4-D. Three cultures (Trichoderma viride. Asperoillus niqer and Isolat E (unidentified)) of the nine cultures are able to grow at 4000 ppm. T. viride grows well on Minimal Basal Media which contained glucose and 2000 ppm 2,4-D and produced the highest biomass (0.8660 g/l) than the others. The biomass of T. viride grew on MBM (without glucose) and added with 2000 ppm 2,4-D is 0,6520g/l. This indicated that the culture is tolerant to 2,4-D and able to use 2,4-D compound as energy and carbon sources for its growth. But we failed to prove the 2,4-D monooxygenase activity of supernatant of T. viride by measuring the changing of pH-value in the 2,4-D breakdown reaction.
PERTUMBUHAN BEBERAPA ISOLAT MIKROBA DARI BERBAGAI LIMBAH INDUSTRI PADA BENZAMIDA Nunik Sulistinah; Bambang Sunarko
BERITA BIOLOGI Vol 5, No 1 (2000)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v5i1.1104

Abstract

Twenty five microbes could be isolated from industrial effluents.Seven isolates of those examined microbes were able to grow on benzamide as sources of carbon,energy,and nitrogen..The highest growth on benzamide was shown by bacterial isolate D1.Besides on benzamide, isolate D1 could grow on acetamide,acrylamide, benzamide,nicotinamide and propionamide, respectively.. On carboxylic acids, however isolate D1 could grow only on acetic acid, propionic acid, and benzoic acid as carbon and energy sources.When isolate D1 grew on 40 mM benzamide, the doubling time(\j was 6 h 40 minutes, the specific growth rate (\J) was 0,046 h'\ the attained maximum cell biomass was 4.96 g cell dry weigtAiter medium, and the yield coefficient (Y) was 124 g cell dry weight/mole benzamide.