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All Journal Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Christanti Sumardiyono
Fakultas Pertanian Universitas Gadjah Mada Yogyakarta
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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EKSPLORASI DAN IDENTIFIKASI TRICHODERMA ENDOFITIK PADA PISANG Taribuka, Johanna; Sumardiyono, Christanti; Widyastuti, Siti Muslimah; Wibowo, Arif
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 16, No 2 (2016): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (19.933 KB)

Abstract

Exploration and identification of endophytic Trichoderma in banana. Endophytic fungi Trichoderma is an organism that can used as biocontrol agent. This study aims to isolate and identify endophytic Trichoderma in roots of healthy banana plants from three districts in Yogyakarta, which will be used as biological control agent against the pathogen Fusarium oxysporum f.sp. cubense. Isolation was conducted using TSM (Trichoderma Selective Medium). We obtained six isolates of endophytic Trichoderma spp., i.e., Swn-1, Swn-2, Ksn, Psr-1, Psr-2, and Psr-3. Molecular identification was done by using ITS1 and ITS2 primer pain and sequenced. The sequence of DNA obtained was analysed and compared with NCBI database by using BLAST-N programe. The results showed that all isolates were amplified at 560-bp. Phylogenetic analysis showed that isolates Swn-1, Swn-2 and Psr-1 are homologous to Trichoderma harzianum, isolate Ksn homologous to Trichoderma asperrellum, isolate Psr-2 homologous to Trichoderma gamsii, and isolate Psr-3 homologous to Trichoderma koningiopsis, with the homologous value of 99%.
EKSPLORASI DAN IDENTIFIKASI TRICHODERMA ENDOFITIK PADA PISANG Taribuka, Johanna; Sumardiyono, Christanti; Widyastuti, Siti Muslimah; Wibowo, Arif
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 16, No 2 (2016): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (188.943 KB) | DOI: 10.23960/j.hptt.216115-123

Abstract

Exploration and identification of endophytic Trichoderma in banana. Endophytic fungi Trichoderma is an organism that can used as biocontrol agent. This study aims to isolate and identify endophytic Trichoderma in roots of healthy banana plants from three districts in Yogyakarta, which will be used as biological control agent against the pathogen Fusarium oxysporum f.sp. cubense. Isolation was conducted using TSM (Trichoderma Selective Medium). We obtained six isolates of endophytic Trichoderma spp., i.e., Swn-1, Swn-2, Ksn, Psr-1, Psr-2, and Psr-3. Molecular identification was done by using ITS1 and ITS2 primer pain and sequenced. The sequence of DNA obtained was analysed and compared with NCBI database by using BLAST-N programe. The results showed that all isolates were amplified at 560-bp. Phylogenetic analysis showed that isolates Swn-1, Swn-2 and Psr-1 are homologous to Trichoderma harzianum, isolate Ksn homologous to Trichoderma asperrellum, isolate Psr-2 homologous to Trichoderma gamsii, and isolate Psr-3 homologous to Trichoderma koningiopsis, with the homologous value of 99%.
Karakterisasi Isolat Rhizoctonia sp. Patogenik dan Rhizoctonia Mikoriza Pada Tanaman Anggrek Tanah Spathoglottis plicata Soelistijono, Soelistijono; Priyatmojo, Achmadi; Semiarti, Endang; Sumardiyono, Christanti
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 16, No 2 (2011): June 2011
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (300.536 KB) | DOI: 10.24002/biota.v16i2.121

Abstract

Rhizoctonia mikoriza merupakan jamur yang mampu berasosiasi dengan anggrek tanah. Selain sebagai mikoriza, terdapat isolat Rhizoctonia sp. patogen dan penyebab penyakit busuk akar pada Spathoglottis plicata. Penelitian ini bertujuan mengetahui perbedaan antara Rhizoctonia sp. patogen dan Rhizoctonia mikoriza secara morfologi dan molekular menggunakan teknik RAPD. Hasil penelitian secara morfologi menunjukkan bahwa warna koloni, panjang dan jumlah inti sel isolat Rhizoctonia sp. patogen dan Rhizoctonia mikoriza pada S. plicata tidak berbeda, tetapi berbeda pada ketebalan sel dan pengelompokan isolat berdasarkan uji anastomosis hifa. Teknik molekuler RAPD menunjukkan bahwa setiap isolat Rhizoctonia sp. patogen dan Rhizoctonia mikoriza memiliki perbedaan pada struktur DNA.
Pengaruh Lengas Nisbi dan Suhu terhadap Kerusakan Gaplek Akibat Aspergillus flavus Selama Penyimpanan Oramahi, H. A.; Sumardiyono, Christanti; Pusposendjojo, Nursamsi; Haryadi, Haryadi
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 2 (2010): June 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (317.519 KB) | DOI: 10.24002/biota.v15i2.2723

Abstract

A study on the spoilage of dried cassava by Aspergillus flavus was still limited so that such study is very important to do. This experiment was done to examine the effect of relative humidity (RH) and storage room temperature on the development of A. flavus and dried cassava spoilage during storage time. Based on the population of A. flavus, starch content, reduction sugar content, and water content of dried cassava, it was concluded that RH 65%, the temperature of 30 and 35oC had better storage condition for dried cassava because it resulted in the lowest population of A. flavus and the lowest deterioration of dried cassava.
Dominansi dan Seleksi Jamur Aspergillus Perusak Gaplek Oramahi, H. A.; Sumardiyono, Christanti; Pusposendjojo, Nursamsi; Haryadi, Haryadi
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 1 (2010): February 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (205.524 KB) | DOI: 10.24002/biota.v15i1.2646

Abstract

Dried cassava is a suitable host for the growth and development of the genus Aspergillus. Fungi reported to grow on dried cassava were A. clavatus, A. flavus, A. foetidus, A. fumigatus, A. niger, A. ochraceus, A. oryzae, A. tamarii, A. zonatus, dan Aspergillus sp. From those species, A. flavus is the most important species because of its toxigenic characteristic on agricultural product. This study was conducted to study dominant species of Aspergillus causing the most severe deterioration on stored dried cassava and causing the highest deterioration in dried cassava. The isolates were then cultured for determination of dominant species. The in vitro experiment was to obtain the species of Aspergillus that is resulted in highest change of dried cassava spoilage. Based on relative index frequency (Rif) and presence index of the fungus (Pif), A. flavus was the most dominant species and causing the highest deterioration on the dried cassava.
Co-Authors A.A. Ketut Agung Cahyawan W Achmadi Priyatmojo Arif Wibowo Endang Semiarti Haryadi - Hasan Ashari Oramahi Johanna Taribuka, Johanna Nursamsi Pusposendjojo, Nursamsi SITI MUSLIMAH WIDYASTUTI Yoga Yuniadi