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PENGARUH PEMBERIAN LIMBAH PABRIK PAKAN (PAKAN CECERAN) YANG DIFERMENTASI DENGAN STARTER FUNGSIONAL TERHADAP PROFIL KOLESTEROL PADA AYAM BROILER (The Effect of Waste Poultry Feeds (Scattered Feed) that was Fermented with Fungsional starteron Cholesterol Pr Fajrina, Ishmah Tri; Sumarsih, Sri; Wahyuni, Hanny Indrat
Animal Agriculture Journal Vol 3, No 3 (2014): Volume 3, Nomor 3, Tahun 2014
Publisher : Animal Agriculture Journal

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Abstract

ABSTRAK Penelitian bertujuan mengkaji pengaruh pemberian limbah pabrik pakan (pakan ceceran) yang difermentasi dengan starter fungsional (terdiri dari ekstrak limbah sayur fermentasi, cairan rumen, pollard dan akuades disebut juga starfungs) terhadap profil kolesterol pada ayam broiler.Materi yang digunakan adalah 105 ekor day old chick (DOC) ayam broiler, pakan limbah pabrik pakan, pakan komersial BR11 dan ‘starfungs’. Penelitian dilakukan menggunakan Rancangan Acak Lengkap (RAL) yaitu 3 perlakuan dengan 7 ulangan.Perlakuan  yang digunakan yaitu T0 = pakan komersil, T1 = pakan tanpa fermentasi dan T2 = pakan fermentasi. Parameter yang diamati meliputi: total kolesterol, low density lipoprotein (LDL) dan high density lipoprotein (HDL) darah ayam broiler. Analisis data yang digunakan adalah analisis ragam dan uji jarak berganda Duncan (UJBD).Hasil penelitian menunjukkan bahwa tidak ada pengaruh nyata pemberian pakan fermentasi limbah pabrik pakan terhadap kadar kolesterol, LDL dan HDL darah. Berdasarkan hasil penelitian dapat disimpulkan bahwa pemberian limbah pabrik pakan yang difermentasi dengan ‘starfungs’ tidak memberikan pengaruh terhadap profil kolesterol darah ayam broiler dilihat dari kadar kolesterol, LDL dan HDL.Kata Kunci: limbah pabrik pakan fermentasi (pakan ceceran); ayam broiler; kolesterol; LDL dan HDL ABSTRACT The research was aimed to study the effect of waste poultry feeds (scattered feed) that was fermented with fungsional starter (that contain extarct of fermented vegetable waste, rumen fluid, pollard and aquades also called starfungs) on cholesterol profile in broiler chickens. The material used was 105 day old broiler chick (DOC), waste poultry feeds, commercial feed BR11 and 'starfungs'. A completely randomized design (CRD) was applied with 3 treatments and 7 replications of each treatment. The treatments measured were commercial feed (T0), feed unfermented (T1) and fermented feed (T2). The parameters observed were: total cholesterol, low density lipoprotein (LDL) and high density lipoprotein (HDL) on broiler blood. Analysis of variance and Duncan multiple range test (DMRT) were used to analysis data. The results of the research showed that there was no significant effect of feeding fermented waste poultry feeds on blood cholesterol levels, LDL and HDL. The conclusion was that waste poultry feeds fermented with starfungs had no effect on blood cholesterol profile of broiler that could be seen on the level cholesterol, LDL and HDL.Keywords: waste poultry feeds (scattered feed); broiler chicken; cholesterol; LDL and HDL
Peningkatan Dalam Menyusun Rencana Pelaksanaan Melalui Supervisi Klinis Pada Kepala SD Daerah Binaan II UPTD Pendidikan Kecamatan Kalikotes Kabupaten Klaten Semester Gasal Tahun Pelajaran 2014/2015 Sumarsih, Sri
Jurnal Varidika: Varia Pendidikan Volume 26 No. 2, Desember 2014
Publisher : Universitas Muhammadiyah Surakarta

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The aim of this study was to describe the increase in the principal’s efforts in preparing the implementation plan through clinical supervision at State Primary School Pa tronage II Regional District of Klaten Kalikotes odd semester of 2014/2015 academic year. Subject and source of research data were five school principals. Methods of data collection were observation, documentation, and testing. Data analysis used a critical and compara- tive analysis. Indicators of success used the minimum 76 and a target of 100% complete- ness. Results of research and discussion suggest that the observation of the principal in the supervision of lesson preparation plan in detail can be described from previsus cycle  to cycle I, there was an increase of 60%, from cycle to cycle II, there is an increase of 80%, and from the first cycle to the second cycle previsus there is an increase of 20%, it can be concluded that the start of the second cycle to previsus cycle is significantly, and principals in the preparation of the lesson plan in detail can be described from previsus cycle to the first cycle, an increase of 60%, from previsus cycle to second cycle, an increase of 100%, and from the first cycle to the second cycle there is an increase of 40%, it can be concluded that the start of the second cycle to before cycle is significantly.
EXPRESSION OF b-XYLOSIDASE ENCODING GENE IN PHIS/ Bacillus megaterium MS SYSTEM Sumarsih, Sri
Indonesian Journal of Tropical and Infectious Disease Vol 2, No 1 (2011)
Publisher : Institute of Topical Disease

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b-Xylosidase encoding gene from G. thermoleovorans IT-08 had been expressed in the pHIS1525/ B. megaterium MS941 system. The b-xylosidase gene (xyl) was inserted into plasmid pHIS1525 and propagated in E. coli DH10b. The recombinant plasmid was transformed into B. megaterium MS941 by protoplast transformation. Transformants were selected by growing the recombinant cells on solid LB medium containing tetracycline (10 µg/ ml). The expression of the b-xylosidase gene was assayed by overlaid the recombinant B. megaterium MS941 cell with agar medium containing 0.2% ethylumbelliferyl-b-D-xyloside (MUX). This research showed that the b-xylosidase gene was succesfully sub-cloned in pHIS1525 system and expressed by the recombinant B. megaterium MS941. Theaddition of 0.5% xylose into the culture medium could increase the activity of recombinantactivity of recombinant of recombinantb-xylosidase by 2.74 fold. The recombinant B. megaterium MS941 secreted 75.56% of the expressed b-xylosidase into culture medium. The crude extract b-xylosidase showed the optimum activity at 50° C and pH 6. The recombinant b-xylosidase was purified from culture supernatant by affinity chromatographic method using agarose containing Ni-NTA (Nickel-Nitrilotriacetic acid). The pure b-xylosidase showed a specific activity of 10.06 Unit/mg protein and relative molecular weight ± 58 kDa.
Exploration of Lipase Enzyme from Soil through Metagenomic Approach Sumarsih, Sri; Baktir, Afaf; Andina, Budi Putri Ayu
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UNEJ e-Proceeding

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This research aimed to explore lipase enzyme from soil through metagenomic approach. The DNA was extracted directly from the topsoil and river sediment by SDS-based lysis method. The soil genomic DNA was sheared into approximately 40-kb fragments The construction of genomic library was carried out by cloning the fragment DNA into  fosmid pCC2FOS vector and host E. coli EPI300-T1R accordance with the protocols of CopyControl™ Fosmid production library kit. Screening of lipase-producing clones was performed based on its lipolytic activity using Luria-Bertani agar plate 12.5 µg/ ml chloramphenicol, 10 µg/ml Rhodamine B and 1 % olive oil as a substrate. The assay plates were incubated at 550 C. The lipolytic activity was identified as an orange around colonies. There were 5 (five) clones of 32 screened-clones that expressed lipolytic activity. The next research will characterize the enzyme and determine the sequence of the enzyme-encoding gene. Keywords: Lipase, soil, metagenomic, fosmid vector, pCC2FOS, E. coli EPI300-T1R
PERAN BARU PUSTAKAWAN DI ERA MEA Sumarsih, Sri
Orbith Vol 13, No 3 (2017): November 2017
Publisher : Orbith

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Peran baru pustakawan di era masyarakat ekonomi Asean (MEA) memberikan dampak kepada para pustakawan untuk berbenah diri dengan meningkatkan kompetensi diri baik dalam meningkatkan kemampuan diri, kemampuan sosial, intelektual dan bidang IT merka untuk dapat bersaing dan sejajar dengan pustakawan dari luar. Adanya persaingan tersebut diperlukan untuk menjadi pustakawan yang smart dalam berinovasi untuk meningkatkan kualitas diri mereka. Strategi yang perlu dilakukan oleh para pustakawan adalah salah satunya dengan memiliki standar kompetensi yang telah ditetapkan oleh Special Libraries Association (SLA) yaitu pustakawan perlu memiliki kompetensi profesional, kompetensi pribadi dan kompetensi Inti atau umum.Kata Kunci: Pustakawan, Kompetensi, MEA
KEBUTUHAN DAN PERILAKU PENCARIAN INFORMASI STAF PENGAJAR POLITEKNIK NEGERI SEMARANG DALAM MELAKSANAKAN KEGIATAN PENELITIAN Sumarsih, Sri
Orbith Vol 13, No 1 (2017): Maret 2017
Publisher : Orbith

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Penelitian ini dengan tujuan untuk mengetahui kebutuhan informasi staf pengajar dalam melaksanakan kegiatan penelitian, perilaku pencarian informasi staf pengajar dalam melaksanakan kegiatan penelitian serta hambatan-hambatan yang dihadapi oleh staf pengajar dalam melaksanakan kegiatan penelitian. Metode penelitian ini adalah metode kualitatif deskriptif. Analisis yang digunakan dalam penelitian ini adalah analisis isi menyajikan hasil akhir dalam bentuk narasi deskriptif. Hasil dari penelitian ini adalah a) Subjek penelitian sesuai dengan bidang kompetensi, b) Sumber informasi yang dibutuhkan buku, jurnal, surat kabar, internet, dan wawancara dengan narasumber, c) Tahun publikasi yaitu terbitan terbaru lima tahun terakhir, akan tetapi tahun publikasi lama juga diperlukan. d) Lokasi sumber informasi diperoleh di luar perpustakaan Polines maupun lembaga terkait dengan bidang yang diteliti, e) Pola pencarian informasi mengikuti pola David Ellis kemudian ditambah dengan menggunakan pencarian informasi melalui akses internet, f) hambatan yang dihadapi paling dominan adalah hambatan dari faktor lingkungan.Kata Kunci : Kebutuhan Informasi, Perilaku Pencarian Informasi, Hambatan dalam Pencarian Informasi, Informasi
KINERJA Saccharomyces cerevisiae REKOMBINAN [GLO1] DALAM PROSES SIMULTAN HIDROLISIS PATI DAN FERMENTASI UNTUK PRODUKSI BIOETANOL Baktir, Afaf; Cholifah, Nur; Sumarsih, Sri
BERITA BIOLOGI Vol 9, No 5 (2009)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

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Abstract

Recent development in fermentations for bioethanol production were focused three factors, i.e. abundance and cheap substrates,superior yeast fermenting the substrates, and simultaneous saccharification and fermentation (SSF) technology.Nowadays national and world bioethanol production still depend on sugar cane and starchy materials.This research aims to determinate the optimum simultaneous saccharification and fermentation (SSF) conditions to identify the performance of local strain Saccharomyces cerevisiae recombinant [GLO1] in the producing bioethanol from starch.The optimum conditions for SSF process are in a media composition containing glucose 2% (w/v), starch 5% and at aeration rate 50 rpm.At these optimum conditions Saccharomyces cerevisiae recombinant [GLO1] produce 25.36% (v/v) bioethanol at day-20 of the fermentation process design.
KEBERADAAN BAKTERI Escherichia coli DAN Salmonella sp PADA LIMBAH PABRIK PAKAN UNGGAS (PAKAN CECERAN) YANG DIFERMENTASI DENGAN STARTER FUNGSIONAL (The Escherichia coli and Salmonella sp in Wastes Poultry Feeds (Scattered Feed) that was Fermented with Fun Hakim, Luqman; Utama, Cahya Setya; Sumarsih, Sri
Animal Agriculture Journal Vol 3, No 3 (2014): Volume 3, Nomor 3, Tahun 2014
Publisher : Animal Agriculture Journal

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ABSTRAKTujuan penelitian yaitu mengkaji keberadaan bakteri Escherichia coli dan Salmonella sp pada limbah pabrik pakan unggas (pakan ceceran) yang difermentasi dengan starter fumgsional (yang terdiri dari ekstrak limbah sayur fermentasi, cairan rumen, pollard dan akuades disebut juga ‘starfungs’). Rancangan yang digunakan adalah Rancangan Acak Lengkap (RAL) dengan 4 perlakuan dan 4 ulangan. Perlakuan yang diamati adalah T0 : pakan ceceran + 0% ‘starfungs’, T1 : : pakan ceceran + 1% ‘starfungs’, T2 : : pakan ceceran + 2% ‘starfungs’, T3 : : pakan ceceran + 3% ‘starfungs’. Parameter yang diamati adalah keberadaan bakteri Escherichia coli dan Salmonella sp pada limbah pabrik pakan (pakan ceceran). Hasil penelitian menunjukkan penambahan ‘starfungs’ mulai level 1% (T1) mampu menekan keberadaan Escherichia coli. Penambahan ‘starfungs’ tidak berpengaruh terhadap keberadaan Salmonella sp. Simpulan penelitian adalah ‘starfungs’ mampu mempengaruhi keberadaan Escherichia coli, akan tetapi tidak dapat mempengaruhi keberadaan Salmonella sp.Kata kunci: limbah pabrik pakan unggas (pakan ceceran); ‘starfungs’; Escherichia coli; Salmonella sp  ABSTRACT The research was aimed to examine the Escherichia coli and Salmonella sp in wastes poultry feeds (scattered feed) that was fermented with fungsional starter (that contain extarct of fermented vegetable waste, rumen fluid, pollard and aquades also called starfungs’).  A completely randomined design was applied with 4 treatment and 4 replications. The treatments measured were T0 : scattered feed + 0% ‘starfungs’, T1 : scattered feed + 1% ‘starfungs’, T2 : scattered feed + 2% ‘starfungs’, T3 : scattered feed + 3% ‘starfungs’. The parameters measured were presence Escherichia coli and Salmonella sp in wastes poultry feeds (scattered feed) that was fermented with ‘starfungs’. Analysis of variance and Duncan multiple range test (DMRT) were used to analyze data. The results of research showed that the ‘starfungs’ began to level 1% (T1) to suppress the Escherichia coli. Additional ‘starfungs’ did not have a significant effect to suppress the Salmonella sp. This could be concluded that ‘starfungs’ could suppress the Escherichia coli started from level 1% (T1), however, it could not suppress the Salmonella sp.Keyword: wastes poultry feeds (scattered feed); ‘starfungs’; Escherichia coli; Salmonella sp
PENGARUH BAKTERI ASAM LAKTAT SEBAGAI STARTER PADA PROSES ENSILASE Sumarsih, Sri
Jurnal Litbang Provinsi Jawa Tengah Vol 13, No 2 (2015): Jurnal Litbang Provinsi Jawa Tengah
Publisher : Badan Penelitian dan Pengembangan Provinsi Jawa Tengah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36762/litbangjateng.v13i2.396

Abstract

The aim of the research was to evaluate the effect of addition starter Lactic Acid Bacteria (LAB) on organoleptic quality of napier grass silage. The completely randomized design was been used on this research with 3 treaments and 5 replications. The treatments were different level LAB ( 0 %, 1 % dan 2% v/w). The parameters were physical organoleptic quality silage (pH, color, odor, texture, and molds). The results showed that physical organoleptic quality of napier grass silage with a starter BAL organoleptic significant (p <0.05) better than without the addition of starter BAL. The conclusion was the use of starter lactic acid bacteria on  ensilage process produces physical organoleptic qualities of good silage.
PENGARUH ARAS UREA DAN LAMA PEMERAMAN YANG BERBEDA TERHADAP SIFAT FISIK AMONIASI ENCENG GONDOK (Bichomia crassipes) Sumarsih, Sri; Tampoebolon, B. I. M.
Jurnal Litbang Provinsi Jawa Tengah Vol 1, No 2 (2003): Jurnal Litbang Provinsi Jawa Tengah
Publisher : Badan Penelitian dan Pengembangan Provinsi Jawa Tengah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36762/litbangjateng.v1i2.33

Abstract

An experiment was done to determine the effect of different level of urea and time of periode amoniation  on properties physically of eceng gondok (Eichornia crassipes) amoniation. The level of urea used were LI= 0%,  L2=3%, L3=5% and 1A=7%. Time of periode amoniation were TI=  0, T2= 14  and T3=28 days. Data were collected from the experiment and analyzed by the analysis of Variance (ANOVA) with Competely Randomized Design with 4 x 3 factorial arrangement. There were 2 replications. The result showed that there were no interaction between level of urea and  time of periode amoniation on properties physically of enceng gondok (Eichomia  crassipes) amoniation. Level urea influence (p<0.05) on the properties physically of enceng gondok (Eichornia crassipes) amoniation).