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All Journal Jurnal Ilmu Pertanian Indonesia Hemera Zoa Media Veteriner Jurnal Sain Veteriner ARSHI Veterinary Letters
Titiek Sunartatie
Department Of Infectious Diseases And Veterinary Public Health, Faculty Of Veterinary Medicine, IPB University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Veterinary

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Eksplorasi Antibakteri dari Kapang Tanah Arboretum Rizal Dwinto Rochman; Titiek Sunartatie; Usamah Afiff
Jurnal Ilmu Pertanian Indonesia Vol. 25 No. 3 (2020): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.25.3.456

Abstract

Research to discover new antibacterial agent has been increasing due to the increasing number of pathogenic bacteria become antibiotic-resistant. Microbiomes in soil mold have antibacterial potency by producing secondary metabolites. The aim of this research was to isolate soil molds from the Faculty of Forestry Arboretum at IPB University. Arboretum’s soil was sampled ± 7.5 g from several points with maximum depth of 15 cm from surface. Mold isolate were cultivated for 28 days using waterbath shaker. Cultivated mycelium and soil filtrate were then separated and extracted using different methods and solvents, prior to antibacterial activity test using agar well diffusion method. Six isolates from Aspergillus, Penicillium, Paecilomyces, and Verticilium were used in this research. Cultivatation using sabouraud dextrose broth (SDB) contains pepton and dextrose in 25°C capable of stimulating mold growth. The research showed the result of secondary metabolit had antibacterial characteristic toward tested bacteria. The results of antibacterial activity test on both mycelium and soil filtrate extract showed that soil mold isolates had antibacterial activity against Staphylococcus aureus and Escherichia coli. Isolate 103A originated from soil filtrate formed the largest inhibition zone on both tested pathogens. Keywords: antibacteria, mycelium, soil filtrate, soil mold
PF-27 Incidence of Feline Dermatophytosis at Dramaga, Bogor in 2013-2018 Agustin Indrawati; Titiek Sunartati; Handina Rakhmawati
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (489.126 KB)

Abstract

Indonesia has a tropical climate and high humidity that give a high prevalence for fungal infections in cat skin such as Feline dermatophytosis (ringworm). Dermatophytosis is the most common fungal infection of cats and one of the most important infectious skin diseases in this species. It may be transmitted to other animal species and also contangious nature, zoonotic potential (Moriello, 2014). Feline dermatophytosis cases are caused by genera Microsporum, Trichophyton, and Epidermophyton. All of these agents produce proteolytic and keratolytic enzymes that enable them to utilize keratin as the sole source of nutrition after colonization of the dead, keratinized portion of epidermal tissue (mostly stratum corneum and hairs, sometimes nails) (Mattei et al., 2014). The aim of this study are to identified and evaluate the kind of fungal that can cause feline dermatofitosis in Dramaga in 2013 until 2018.
The Inhibitory Effect of Aflatoxin on The Growht of Bacillus megaterium and Staphylococcus epidermidis Eko Sugeng Pribadi; Unang Patriana; Titiek Sunartatie
Media Veteriner Vol. 5 No. 1 (1998): Media Veteriner
Publisher : Media Veteriner

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (451.653 KB)

Abstract

B. megaterium and S. epidermidis were used to study the inhibitory effect or pure aflatoxin and aflatoxin-contaminated rice. Results showed that the inhibitory effect was more pronounced In B. megatarium growth. The inhibitory effect of pure aflatoxin started at the concentration of 20 μg/ml and persisted to the concentration of 25 μg/ml (P
Trichophyton mentagrophytes sebagai Agen Penyebab Dermatofitosis pad Kambing Titiek Sunartatie
Jurnal Sain Veteriner Vol 28, No 1 (2010): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2496.292 KB) | DOI: 10.22146/jsv.459

Abstract

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Sensitivitas antimikroba dari bakteri terisolasi yang paling umum pada Feline Upper Respiratory Infection Karen Lee; Usamah Afiff; Safika Safika; Titiek Sunartatie
ARSHI Veterinary Letters Vol. 5 No. 3 (2021): ARSHI Veterinary Letters - Agustus 2021
Publisher : School of Veterinary Medicine and Biomedical Sciences, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avl.5.3.55-56

Abstract

Pada kucing, infeksi saluran pernapasan atas (URI) dapat dikaitkan dengan infeksi bakteri primer atau sekunder dan umumnya dilakukan pengobatan dengan antimikroba. Penggunaaan antimikroba yang tidak tepat, dan penggunaannya berlebihan. Tidak ada protokol rinci untuk pengobatan, seperti yang tersedia untuk pengobatan manusia. Tujuan penelitian ini adalah untuk mengetahui kepekaan antimikroba dari bakteri yang diidentifikasi pada kucing yang terinfeksi URI. Dilakukan isolasi dan identifikasi bakteri pada sampel kucing yang terinfeksi URI kemudian dilanjutkan dengan uji kepekaan antibiotik amoksisilin, doksisiklin, tetrasiklin, azitromisin, siprofloksasin, dan sefotaksim dengan metode difusi Kirbey Bauer Agar Dics. Berdasarkan hasil penelitian, bakteri yang dapat diidentifikasi adalah Enterobacter spp, Streptococcus spp, Staphylococcus spp, Pseudomonas spp, Seratia spp, Yersinia spp, Micrococcus spp, Klebsiella spp dan Hafnia spp. Ditemukan resistansi antibiotik amoksisilin pada empat isolat Staphylococcus spp dan dua isolat resistan terhadap sefotaksim. Resistansi antibiotik amoksisilin dan tetrasiklin ditemukan pada satu isolat Streptococcus spp, dua isolat resistan terhadap sefotaksim serta dua isolat intermediet terhadap doksisiklin. Satu isolat bakteri Enterobacter spp resistan terhadap amoksisilin, azitromisin dan dua isolat resistan terhadap sefotaksim. Semua isolat yang diuji sensitif terhadap siprofloksasin. Berdasarkan uji kepekaan antibiotik, sebagian besar isolat bersifat sensitif, namun terdapat ada isolat yang resistan terhadap antibiotik, terutama amoksisilin dan sefotaksim.
Co-Authors Agustin Indrawati Handina Rakhmawati Karen Lee Rizal Dwinto Rochman Safika S, Safika Unang Patriana Usamah Afiff