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All Journal Jurnal Sain Veteriner Jurnal Veteriner Aceh Journal of Animal Science Jurnal Medik Veteriner Journal of Parasite Science Ovozoa: Journal of Animal Reproduction journal of Basic Medical Veterinary

Endang Suprihati

Division Of Veterinary Parasitology, Faculty Of Veterinary Medicine, Universitas Airlangga

Author-ID : 393918

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

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Variasi Morfologi dan Deteksi Leucocytozoon caulleryi dengan Metode PCR pada Ayam Ras di Wilayah Endemis Indonesia Endang Suprihati; Wiwik Misaco Yuniarti
Jurnal Sain Veteriner Vol 35, No 2 (2017): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

The purpose of this study was to analyze the variation in morphology and detection Leucocytozoon caulleryi by PCR in mtDNA genes cty b that attacking bred chickens in endemic areas of Indonesia. This study was divided into three stages, data collection of Leucocytozoonosis cases in endemic areas of Indonesia; identification of parasites Leucocytozoon caulleryi morphologically through microscopic examination; and the identification of Leucocytozoon caulleryi cyt b genes that attack bred chickens in endemic areas by PCR. The results showed that there are variations in morphology of Leucocytozoon caulleryi that attacking chicken in endemic areas of Indonesia. Gamete morphometry of L. caulleryi had an average length and width 18.233 ± 4.672 dan 12.934 ± 3.349 µm. Nested PCR clearly showed positive reaction of Leucocytozoon infections by amplicons in 600 bp and 03 bp length.

Deteksi Cryptosporidium canis pada Anjing di Kota Surabaya (CRYPTOSPORIDIUM CANIS DETECTION IN DOGS IN THE CITY OF SURABAYA) Romy Muhammad Dary Mufa; Nunuk Dyah Retno Lastuti; Fedik Abdul Rantam; Lucia Tri Suwanti; Endang Suprihati; Didik Handijatno; Mufasirin Mufasirin
Jurnal Veteriner Vol 21 No 2 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (138.954 KB)

Cryptosporidiosis is a disease caused by Cryptosporidium spp. protozoan parasites and are zoonotic. Cryptosporidium canis is the main species that infects dogs. Transmission of C. canis in dogs to humans is possible. This study aims to detect microscopic C. canis infection based on morphology and molecularity using the Polymerase Chain Reaction (PCR) in dogs in Surabaya City. A total of 80 diarrhea dog feces samples were taken from Animal Hospitals and animal clinics in several areas in the Surabaya City, then added potassium dichromate and stored at 4OC. Detection was made of the presence of Cryptosporidium spp. oocysts microscopically which is then confirmed by molecular examination using the PCR method. The results showed 40 positive samples containing Cryptosporidium spp., oocysts, with a size of 2-6 ?m. Ten samples from the total positive sample of Cryptosporidium spp. oocysts by microscopic examination, with the PCR test there were seven positive samples of C. canis. Based on the results of the study it can be concluded that the species that causes Cryptosporidiosis in dogs in Surabaya City is C. canis. The high cases of Cryptosporidiosis in dogs can be a warning to be able to prevent Cryptosporidium spp. infections, especially in pets that have the potential as a reservoir in spreading disease.

Deteksi Molekuler Blastocystis sp. pada Babi Terinfeksi di Kabupaten Tabanan dan Badung, Provinsi Bali, Indonesia (MOLECULAR DETECTION OF BLASTOCYSTIS INFECTION IN PIGS AT TABANAN AND BADUNG DISTRICT, BALI PROVINCE, INDONESIA) Doohan Mahendra; Lucia Tri Suwanti; Nunuk Dyah Retno Lastuti; Mufasirin Mufasirin; Endang Suprihati; Wiwik Misaco Yuniarti; Ni Komang Aprilina Widisuputri
Jurnal Veteriner Vol 21 No 2 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (141.288 KB)

This study aims to identify morphology and molecular of Blastocystis infection in pigs in the Tabanan and Badung District, Bali Province, Indonesia. 100 samples of pig stool were taken, fifty samples from the Tabanan and fifty samples from Badung District. Blastocystis sp. isolated and identified microscopically and confirmed by polymerase chain reaction (PCR) as a molecular identification method. 63% (63/100) Blastocystis is found in pig feces from these two District by microscopic detection. Positive samples were found 27 samples from Badung and 36 samples from Tabanan. Five samples were taken from each District conducted a confirmation test using the PCR method and found 60% (6/10) positive samples, two samples from Badung and four samples from Tabanan. The results of this study indicate that in healthy pigs, they also contain parasites that have the potential to spread disease to humans. Therefore, the high incidence of Blastocystis in pigs can be an alarm or warning to prevent Blastocystis infection from livestock that can potentially to be a reservoir in the spread of disease.

Deteksi Molekuler Entamoeba suis pada Babi di Rumah Potong Hewan Pegirian Surabaya Fransiska Cicilia Beka; Nunuk Dyah Retno Lastuti; Lucia Tri Suwanti; Koesdarto Koesdarto; Mufasirin Mufasirin; Endang Suprihati; Dyah Ayu Kurniawati
Jurnal Veteriner Vol 22 No 3 (2021)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Penelitian ini bertujuan untuk mendeteksi E. suis pada babi yang dipotong di Rumah PotongHewan (RPH) Pegirian, Surabaya. Sebanyak 63 sampel feses babi dikoleksi dari RPH Pegirian Surabaya. Deteksi morfologis Entamoeba spp. dilakukan dengan metode apung (analisis parasitologi). Selain itu, penentuan spesies E. suis dilakukan dengan PCR menggunakan primer Esuis764 (5’-ATC AAA TCA ATT AGG CAT AAC TA-3’) dan Esuis765 (5’-AAT TAA AAC CTT ACG GCT TTA AA-3’) dan prodak PCR yang diharapkan adalah 320 bp. DNA dari sampel positif diekstraksi dengan DNAzol. PCR dilakukan pada beberapa sampel positif yang dipilih secara acak dari analisis parasitologi. Hasil penelitian menunjukkan 57,14% (36/63) sampel secara morfologis positif Entamoeba sp. Hasil penentuan spesies berdasarkan analisis molekuler menemukan bahwa protozoa yang menginveksi babi di Rumah Potong Hewan Pegirian, Surabaya adalah E. suis.

Vaksininasi Protein Ekskretori-Sekretori Toxoplasma gondii Hasil Biakan in vivo Membangkitkan Respons Imun Non Protektif (THE VACCINATION OF Toxoplasma gondii EXCRETORY-EXCRETORY PROTEINS FROM IN VIVO CULTURE ENHANCED IMMUNE RESPONSE UNABLE PROTECTIVE) Mufasirin .; Endang Suprihati
Jurnal Veteriner Vol 14 No 1 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (119.743 KB)

The aims of this research was to explore the immune response and protectiveness of mice whichwere vaccinated with Toxoplasma gondii excretory-secretory proteins produced from in vivo culture. A totalof 50 Balb/c strain mice were allotted into five groups. Mice in group 1 to 3 were vaccinated with 20.7 kDa,35.3 kDa, and 100.9 kDa of the protein, respectively. Whereas mice in group 4 were given total excretory-secretory antigen ( total ESA5), and mice in group 5 were used as control (PBS). Booster vaccinated wasconducted at two weeks following the first vaccination. Prior the challenge test, five mice were sacrificed forimmunologlobulin-G (IgG) analysis. The analysis of IgG using enzyme linked immunosorbent assay (ELISA).Challenge test used 1x103 T. gondii tachyzoites which were given to the animals intraperitoneally. Theresults showed that vaccination using T.gondii excretory-secretory proteins as well as T. gondii total ESAscould enhanced immune response as detected by the markedly presence of T. gondii IgG. However, thiswas unable to protect against T. gondii RH strain infection.

Zoonotic potential of gastrointestinal parasite in long-tailed Macaque Macaca fascicularis at Baluran National Park, Situbondo, East Java, Indonesia Dyah Ayu Kurniawati; Lucia Tri Suwanti; Nunuk D.R. Lastuti; Setiawan Kusdarto; Endang Suprihati; Mufasirin Mufasirin; Arif Pratiwi
Aceh Journal of Animal Science Vol 5, No 1 (2020): July 2020
Publisher : Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/ajas.5.1.15397

Baluran National Park (BNP) is one of the highest number of tourist visit among Indonesian national park. In the past decades, excessive feeding has induced change in macaque behaviour which increased the number of recorded human-macaque interaction. The close contact between macaque and humans can increase the risk of disease transmissions. This study aimed to identify gastro intestinal (GI) parasite in the long-tailed macaque. To provide identification, we adopted morphologic methods. We collected 100 faeces from unidentiﬁed individuals of Long-tailed macaque in BNP. Fecal samples were tested using direct smear and modified sugar floatation techniques. Microscopic examination showed 89% (89/100) samples were found to be positive of GI parasite. The prevalence of protozoa infection was higher (89%) than helminth (83%). The most prevalent GI parasite is Trichostrongylus sp (66%) following with Entamoeba sp. (53%), Strongyloides sp. (32%), Blastocystis sp. (32%), Trichuris sp.(17%), Giardia sp. (10%) and Enterobius sp. (3%). All of GI parasite that successfully identified have zoonotic concern. In conclusion, GI parasites found in faeces of long-tailed macaque at Baluran National Park potentially a zoonotic transmission.Keywords : GI Parasite; Long tailed Macaque; Baluran National Park; Zoonosis

Infestation Pattern of Lice In Laying Ducks In Village of Kramat District of Bangkalan Region of Bangkalan Kiki Amalia Rama; Poedji Hastutiek; Oky Setyo Widodo; Endang Suprihati; Agus Sunarso; Soeharsono Soeharsono
Journal of Parasite Science (JoPS) Vol. 1 No. 2 (2017): Journal of Parasite Science
Publisher : Universitas Airlangga

The purpose of this research is to identify lice and to know the pattern of infestation of lice that infest laying ducks in Village of Kramat, District of Bangkalan, Region of Bangkalan. Forty samples of laying ducks were taken in Kramat Village and identified in Entomology and Protozoology Laboratory of Parasitology Department of Veterinary Faculty of Airlangga University, the research was conducted from July to September 2017. This study used an explorative observational research design. Lice taken from a sample of laying ducks are fed into an ointment pot containing 70% alcohol to preserve lice and labeled in accordance with the infected duck's limb region. Identification of lice using Permanent mounting method without coloration. The results of identification, the lice that infest laying ducks consists of three types of Anaticola crassicornis 60%, Menacanthus stramineus 25% and Lipeurus caponis 17.5%. The results of infestation pattern, infected body's region is head-neck, wings and back. The wings infested two types of lice were A. crassicornis and L. caponis, head-neck infested M. stramineus and in the infected back region of A. crassicornis.

The Conformity of Diagnostic Test Between Burrow Ink Test Method With Skin Scrapping Method of Scabies in Rabbit (Lepus domesticus) Fadila Zikri Amanda; Poedji Hastutiek; Emy Koestanti Sabdoningrum; Nunuk Dyah Retno Lastuti; Endang Suprihati; Hana Eliyani
Journal of Parasite Science (JoPS) Vol. 2 No. 1 (2018): Journal of Parasite Science
Publisher : Universitas Airlangga

This study intend to know the conformity of diagnostic test between burrow ink test method with skin scrapping method of scabies in rabbit. Scabies caused by Sarcoptes scabiei which has a predilection in stratum corneum and lucidum. Clinical symptoms was characterized by papula or crusting of the skin. This study presents a laboratory explorative research with cross sectional approach. Samples used 20 rabbits that infested scabies naturally. Data obtained by performing diagnostic burrow ink test method and skin scrapping in rabbits which are infested scabies naturally. KAPPA test used as data analysis. Results showed that the number of positive results based on burrow ink test method are 12 rabbits and 8 rabbits are negative while skin scrapping obtained the positive results are 11 rabbits and 9 rabbits are negative. KAPPA test analysis performed on the overall study data was obtained 0,490. The conclusion of this study is burrow ink test method has a good conformity with the skin scrapping in rabbits which are infested scabies naturally that can be used for diagnostic analysis of scabies with a simple method, faster, and have the same ability with skin scrapping without hurting animals.

Prevalance of Gastrointestinal Protozoa of Cats in Animal Hospital and Animal Clinic in Surabaya Amara Lintang Pagati; Lucia Tri Suwanti; Chairul Anwar Nidom; Wiwik Misaco Yuniarti; Sarmanu Sarmanu; Endang Suprihati
Journal of Parasite Science (JoPS) Vol. 2 No. 2 (2018): Journal of Parasite Science
Publisher : Universitas Airlangga

Research of gastrointestinal protozoan in cats at Surabaya are still limited. Protozoa infection of the gastrointestinal tract can cause diarrhea and even zoonosis. This research aimed to identify and determine the prevalence of protozoan in cats in animal hostptal and animal clinic in Surabaya. Ninety fecal samples were collected from 2 animal clinic and one animal hospital. Samples were examined e wet mount (native, sedimentation, and floatation) and (Ziehl Nellsen) stain. Protozoa was identified by using a light microscope with 400x and 1000x magnification. The result showed 68,89% of samples were positively infected by gastrointestinal protozoa. The protozoa were Blastocystis sp, Cryptosporidium sp, Giardia sp, and Eimeria sp. By chi square test, there was not significant differences the prevalence of gastrointestinal protozoan in cat between sex, age, breed, and diarrhea status

Microscophy Identification of Toxocara cati First Stage Larvae and Second Stage Larvae Eny Coolfina Simarmata; Kusnoto Kusnoto; Mochamad Lazuardi; Setiawan Koesdarto; Endang Suprihati; Kuncoro Puguh Santoso
Journal of Parasite Science (JoPS) Vol. 3 No. 1 (2019): Journal of Parasite Science
Publisher : Universitas Airlangga

This study was aimed to identify the ultra structural morphology of Toxocara cati First Stage Larvae and Second Stage Larvae using Light Microscope. Toxocara cati larvae were obtained from adult worm eggs then were treated in phospat buffer saline with comparasion 1 : 1 until it reached the stage of larvae 1 and 2. The shell of egg Toxocara cati were thick and usually consist of three layers. The first layer was inner membrane, the second layer was middle layer and the third was outer layer. The results of larvae morphology that were identified using light microscope showed that the anterior part of Toxocara cati first stage larvae has a dorsal lip and esophagus and intestine on the posterior part but it could not be identified perfectly. The anterior part of Toxocara cati second stage larvae that were identified has three lips that leads directly into the oesophagus. Three lips on the anterior part of Toxocara cati larvae 2 consist of a dorsal lip and two subventral lips. Morphological of Toxocara cati larvae similar with the Toxocara cati adult worms because morphology of larvae and adult stage was difficult to differentiated.

Co-Authors Aditya Yudhana Afiyah Rahmadani Agnes Theresia Soelih Estoepangestie Agus Sunarso Akbar Wijaya Putra Purnama Amara Lintang Pagati Amelia Dwita Safitri Andaru Rizki Arif Pratiwi Arif Pratiwi Arimbi Arimbi Azizah Bilqis Nurkarimah Boedi Setiawan Cahaya Panjaitan Chairul Anwar Nidom David Mohamad Qadafi Dhio Asmaydo Dian Ayu Permatasari Didik Handijatno Doohan Mahendra Dyah Ayu Kurniawati Dyah Ayu Kurniawati Dyah Ayu Kurniawati Ellza Agatha Damayanti Emy Koestanti Sabdoningrum Eny Coolfina Simarmata Erma Safitri Fadila Zikri Amanda Faisal Fikri Fedik Abdul Rantam Firman Hadi Fanani Fransiska Cicilia Beka Ghifari Lutfi Fauzi Hana Eliyani Hani Plumeriastuti Hartono Hartono Ismudiono Ismudiono Iwan Sahrial Hamid Jihaan Haajidah Kiki Amalia Rama Koesdarto Koesdarto Koesnoto Supranianondo Kuncoro Puguh Santoso Kusnoto Kusnoto Kusriningrum Rochiman Lianny Nangoi Lilik Maslachah Lilik Maslachah Lucia Tri Suwanti, Lucia Tri Marchelia Arifiandani Maudika Pamela Fiorenza Meta Aprilia Meyreta Doti Alcaterana Mochamad Lazuardi Mohammad Anam Al Arif Mohammad Sukmanadi Mufasirin Muhammad Fahmi Abdillah Muhammad Hambal Muhammad Thohawi Elziyad Purnama Mustofa Helmi Effendi Nenny Harijani Ni Komang Aprilina Widisuputri Nining Sari Virgandina Vinola Nizar Bachrudin Prihandono Nunuk D.R. Lastuti Nunuk Dyah Retno Lastuti Nunuk Dyah Retno Lastuti Nurin Ardhiani Oky Setyo Widodo Poedji Hastutiek Prima Ayu Wibawati Pudji Srianto Rahmi Sugihartuti Ratna Damayanti Restiadi, Tjuk Imam Retno Wulansari Rochmah Kurnijasanti Romy Muhammad Dary Mufa Sarmanu Sarmanu Setiawan Koesdarto Setiawan Kusdarto Soeharsono Soeharsono Sri Mumpuni Sosiawati Sunarso, Agus Sunaryo Hadi Warsito Suzanita Utama Trilas Sardjito Warsito, Sunaryo Hadi Wiwik Misaco Yuniarti Zerlinda Dyah Ayu

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