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All Journal Jurnal Kimia Terapan Indonesia Seminar Nasional Insinyur Profesional (SNIP)

A. Sidik

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Author-ID : 3944110

Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Engineering Industrial & Manufacturing Engineering Materials Science & Nanotechnology

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FERMENTASI ALFA AMILASE DARI ASPERGILLUS ORYZAE PADA MEDIA SAGU METROXYLON Yetti M. Iskandar; Dine Agustine; A. Sidik; Linar Z.Udin; A. T. Karossi
Jurnal Kimia Terapan Indonesia Vol 4, No 1 (1994)
Publisher : Research Center for Chemistry - LIPI

Alpha amylase is an extracellular enzyme which can be obtained from Aspergillus oryzae fermentation. The production of the alpha amylase in fermentation of sago starch media (Metroxylon sp)in 600, 800 and 1500 ml scale at 30°C for 7 to 9 days in aerobic condition has been conducted. The observations at day-3 indicated that the maximum enzyme specific activity assayed at 40°C for 30 minutes incubation, was 1096 U/g protein, 963 U/g protein and 810 U/g protein for 600, 800 and 1500 ml scale respectively. At this condition starch utilization for growth reached 69% for the 600 and 800 ml scale and 71% for the 1500 ml scale and the biomass production was 6.03 g dry weight/L media, 4.03 g dry weight/L media and 5.66% g dry weight/L media for the 600, 800 and 1500 ml scale respectively.

PENGARUH CARA PENAMBAHAN ENZIM GLUKOAMILASE DAN ION LOGAM ALKALI DAN ALKALI TANAH PADA PROSES SAKARIFIKASI PATI SAGU A. T. Karossi; Agus Muchliawan; Linar Z.Udin; A. Sidik
Jurnal Kimia Terapan Indonesia Vol 5, No 1 (1995)
Publisher : Research Center for Chemistry - LIPI

Production of glucose syrup enzymatically employs both alpha-amylase and glucoamylase. Alpha amylase acts during liquifaction while glucoamylase in the saccharification process. In the present study the glucoamylase was obtained from Rhyzopus oryzae fermentation for five days using 4 liter scale LKB fermentor. The influence of single stage and multiple stage additions of glucoamylase on alpha amylase liquified sago starch indicated no significant difference on the saccharification. The presence of 0.2 - 0.8 mM Li+, Na+, K+, Mg++, Ca++ and-Bar+ salts enchanced the glucoamylase activity whereas at the level of 1 mM they acted as inhibitors. The results of HPLC analysis of the saccharification product showed that the glucoamylase hydrolysed 83.3% of the sago starch yielding free glucose.

PEMURNIAN GLUKOAMILASE DARI HASIL FERMENTASI KAPANG RHIZOPUS ORYZAE Linar Z. Udin; Rini Noviyanti; A. Sidik; A. T. Karossi
Jurnal Kimia Terapan Indonesia Vol 4, No 1 (1994)
Publisher : Research Center for Chemistry - LIPI

Purification of the glucoamylase R. oryzae was carried out by addition of ammonium sulfate 80% saturation, on the fermentation broth at 4°C. The precipitate formed by centrifugation at 9000 rpm was then dialyzed in buffer solution and then concentrated using freeze dryer. It was found that the specific activity of the enzyme was around three-fold higher the crudeenzyme from fermentation broth and the purity of the enzyme was almost twelve-fold.purer than the crude enzyme. The molecular weight of the glucoamylase was found to be 36,000 as determined by SDS gel electrophoresis. The optimum pH witli soluble starch as substrate was at pH 4.5 and the optimum temperature was 55°C while the Km Value was 0.027%.

APLIKASI MEMBRAN DALAM PEMEKATAN ENZIM GLUKOAMILASE A. Syahril; S Nana; L. Z. Udin; A. Sidik; A. T. Karossi
Jurnal Kimia Terapan Indonesia Vol 3, No 1 (1993)
Publisher : Research Center for Chemistry - LIPI

Preparation of glucoamylase enzyme by fermentation of sago and soy bean meals had been done. Enzyme concentration was carried out by ultrafiltration process, where polysulfone membranes were used as medium filter. Membranes used in this experiment were prepared with several treatments, such as coagulation temperature and composition and beside that effect of solvent and additive are also observed. From the results of the observation during fermentation process in enzyme preparation, its clear that pH of solution changed in that pH increased with increasing fermentation time. The highest enzyme activity was shown on the sixth day of fermentation namely 2.908 U/ml with a specific enzyme activity of 1495.8 U/g protein. There is fluctuation in protein content during [ermenuuion process, but the highest level was obtained on the tenth day [ermenuuion (3.102 mg/l). The highest specific enzyme activity was shown on the sixth day fermentation (1495.8 U1g protein). The best membrane for the enzyme concentration by ultrafiltration process in this experiment are found form the membrane prepared from dimethyl acetamide as solvent and polyvinylpyrrolidane as additive. This membrane gave rejection coefficient of more than 90% andflux as much as 20.571 l/m2.hour.

Evaluasi Pekerjaan Jembatan Sementara Dengan Menggunakan Box Culvert Dan CSP (Corrugated Steel Pile) Sebagai Penunjang Pelaksanaan Pekerjaan Bendungan Margatiga A. Sidik
Seminar Nasional Insinyur Profesional (SNIP) Vol. 1 No. 1 (2021): Prosiding SNIP Vol.1 No.1
Publisher : Fakultas Teknik

Bangunan penunjang merupakan bangunan atau fasilitas yang dibangun untuk menunjang pelaksanaan pekerjaan konstruksi agar pekerjaan dapat terlaksana sesuai dengan rencana. Salah satu bangunan penunjang pada pelaksanaan bendungan adalah jembatan sementara yang digunakan untuk akses penyebrangan. Bangunan jembatan sementara berfungsi sebagai akses jalan yang menghubungkan pekerjaan yang ada di main dam kanan dan kiri bendungan. Pada Bendungan Margatiga yang terletak di Lampung Timur, terdapat pelaksanaan pekerjaan jembatan sementara menggunakan Box Culvert dan CSP (Corrugated Steel Pipe). Pelaksanaan pekerjaan jembatan Box Culvert dan CSP (Corrugated Steel Pipe), dibangun dengan beberapa yang ditinjau diantaranya aspek penampang basah, waktu pelaksanaan, dan biaya pekerjaannya. Jembatan boxculvert merupakan jembatan sementara untuk akses pekerjaan ke sisi tengah main dam dan jembatan CSP (Corrugated Steel Pipe) merupakan jembatan sementara untuk akses untuk penyebrangan ke sisi kanan main dam. Dengan melakukan evaluasi pelaksanaan pekerjaan jembatan sementara tersebut diharapkan dapat memberikan gambaran evaluasi efektifitas pembangunan pekerjaan tersebut sebagai pekerjaan penunjang sehingga diketahui efisiensipelaksanaan pekerjaannya. Evaluasi pelaksanaan jembatan sementara ini diharapkan menjadi referensi dalam menentukan pembangunan jembatan sementara kedepannya.

Co-Authors A. Syahril A. T. Karossi Agus Muchliawan Agustine, Dine L. Z. Udin Linar Z. Udin Linar Z.Udin Rini Noviyanti S Nana Yetti M. Iskandar

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