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Yetti M.lskandar
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Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Materials Science & Nanotechnology

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PENGGANDAAN SKALA PRODUKSI ENZIM GLUKOAMILASE DARI LABU KOCOK KE FERMENTOR 10 L Patuan L. P. Siagian; A. T. Karossi; Yetti M.lskandar; Tigor N. Surawidjaja; Ngadiman Ngadiman
Jurnal Kimia Terapan Indonesia Vol 4, No 2 (1994)
Publisher : Research Center for Chemistry - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4518.73 KB) | DOI: 10.14203/jkti.v4i2.285

Abstract

Glucoamylase was produced in a 10 L stirred tank fermentor using Rhizopus oryzae L16 and sago (Metroxylon spy starch. Fermentation conditions were adapted from the results obtained from shake flask (250 mL) and 4L fermentor experiments. At the present investigation, the temperature was set at 30°C, pHs were at a constant value of 4.0, 4.5, 5.0, 5.5, and 6.0. Agitation rate at aeration 1.5 vvm were adjusted to 286 rpm, 300 rpm, and 350 rpm (medium volume 6 L). The maximum production of glucoamylase was reached at agitation rate 350 rpm, aeration 1.5 wm and pH 4.0. At day-s, the glucoamylase activity was 2,285 U/L and its specific activity was 9,326 U/g protein. At day-5 the specific activity increased to 13,631 U/g protein. This maximum production was reached at an average kLa of43 h(-1)
RADIASI SINAR ULTRA VIOLET STRAIN ASPERGILLUS ORYZAE UNTUK MENINGKATKAN PRODUKSI ALFA AMILASE Yetti M.lskandar; Linar Z. Udin; A. T. Karossi
Jurnal Kimia Terapan Indonesia Vol 5, No 1 (1995)
Publisher : Research Center for Chemistry - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2699.427 KB) | DOI: 10.14203/jkti.v5i1.244

Abstract

Mutation of Aspergillus oryzae was carried out by ultra violet irradiation at 254 nm. The mutans obtained with 0, 10, 20,30, 40 and 50 minutes irradiation were screened for their amylolytic activity and alpha amylase production. The latter was carried out by aerobic fermentation using sago (Metroxylon spy starch in shake flasks for five days at 30°C with orbital shake at 120 rpm. The observation indicated that the mutant resulted from 10 minute irradiation demonstrated a maximum alpha amylase activity of 1675 Unit/g protein at day-d. The amylase activity was assayed at 40°C for 30 minute incubation. The starch utilization was 87% and 3.84 g dry weight of biomass per L medium was produced. The specific activity of alpha amylase obtained from untreated parental strain was 1069 Unitlg protein. Starch consumption and biomass production was 80% and 3.62 g dry weight/ L medium, respectively. The increase of alpha amylase specific activity at 10 minute irradiation time was 56%.
PRODUKSI GLUKOAMILASE DARI RHIZOPUS ORVZAE L16 PADA MEDIA PATI SAGU (METROXYLON) YANG MENGANDUNG EKSTRAK TAUGE Yetti M.lskandar; Linar Z.Udin; A. T. Karossi
Jurnal Kimia Terapan Indonesia Vol 4, No 2 (1994)
Publisher : Research Center for Chemistry - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2079.861 KB) | DOI: 10.14203/jkti.v4i2.287

Abstract

Glucoamylase production from Rhlzopus oryzae L16 has been carrried out in a fermentation medium using sago (Metroxylon sp) starch. Various levels of mung bean sprout extract (1% - 5%) was added into the medium as vitamin source. The fermentation process was carried out at 30°C in aerobic condition with an agitation rate of 120 rpm and for five days. The results of the fermentation was compared with an other fermentation medium containing malt extract 3%. The glucoamylase specific activity of 4500 Unitslg protein, starch consumption of 62.5% and biomass produced of 2.93 g dry weighill: medium were demonstrated with the latter medium. It was found that media which contained 4% mung bean sprout extract had maximum glucoamylase specific activity of 19720 Unitslg protein at day-3. The enzyme activity was assayed at 55°C with incubation time 10 minutes. At this third day of fermentation the starch utilization reached 55.5% and the biomass production was 3.12 g dry weight/L medium.
Co-Authors A. T. Karossi Linar Z. Udin Linar Z.Udin Ngadiman Ngadiman Patuan L. P. Siagian Tigor N. Surawidjaja