Article Per Year (5 Year)
p-Index From 2019 - 2024
0
P-Index
This Author published in this journals
All Journal Jurnal Kimia Terapan Indonesia
L. Z. Udin
Unknown Affiliation
Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Materials Science & Nanotechnology

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
APLIKASI MEMBRAN DALAM PEMEKATAN ENZIM GLUKOAMILASE A. Syahril; S Nana; L. Z. Udin; A. Sidik; A. T. Karossi
Jurnal Kimia Terapan Indonesia Vol 3, No 1 (1993)
Publisher : Research Center for Chemistry - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3340.801 KB) | DOI: 10.14203/jkti.v3i1.297

Abstract

Preparation of glucoamylase enzyme by fermentation of sago and soy bean meals had been done. Enzyme concentration was carried out by ultrafiltration process, where polysulfone membranes were used as medium filter. Membranes used in this experiment were prepared with several treatments, such as coagulation temperature and composition and beside that effect of solvent and additive are also observed. From the results of the observation during fermentation process in enzyme preparation, its clear that pH of solution changed in that pH increased with increasing fermentation time. The highest enzyme activity was shown on the sixth day of fermentation namely 2.908 U/ml with a specific enzyme activity of 1495.8 U/g protein. There is fluctuation in protein content during [ermenuuion process, but the highest level was obtained on the tenth day [ermenuuion (3.102 mg/l). The highest specific enzyme activity was shown on the sixth day fermentation (1495.8 U1g protein). The best membrane for the enzyme concentration by ultrafiltration process in this experiment are found form the membrane prepared from dimethyl acetamide as solvent and polyvinylpyrrolidane as additive. This membrane gave rejection coefficient of more than 90% andflux as much as 20.571 l/m2.hour.
PRODUKSI ALFA-AMILASE OLEH ASPERGILLUS ORYZAE DALAM MEDIA PATI SAGU (Metroxylon sp.) S. Pudjiraharti; L. Z. Udin; A. T. Karossi
Jurnal Kimia Terapan Indonesia Vol 7, No 1-2 (1997)
Publisher : Research Center for Chemistry - LIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3400.641 KB) | DOI: 10.14203/jkti.v7i1-2.221

Abstract

The production 0f alpha-amylase in sago starch media by A. oryzae have-been performed in Biostat-B stirred tank fermentor with working volume of 2 L. The condition was adapted from the fermentation using Biotech fermentor: working volume 4 Liters, temperature 27°e, aeration 0,75 wm and agitation of 300 rpm. The concentrations of inoculum added into the medium were 2,5 - 3% v|v. The maximum enzyme speslfic activites around 300-460 U|g protein was obtained at fermentation using inoculum concentration of 2,5%, while the maximum enzyme specific activity of 850 U|g protein was also obtained at fermentation using inoculum concentration of 3%. The maximum enzyme specific activity was achieved at day 5 or 6 of fermentation.Fermentation using various concentrations of inoculum in erlenmeyer flask scale was carried out to investigate the inoculum concentration which resulting maximal enzyme activity. The concentrations used were 5.0%; 7.5%; 10%; and 12.5% v|v. Fermentation was done at 30°C and agitation of 120 rpm. The highest enzyme activity of 12,640 Vlg protein was resulted at fermentation with inoculum concentration of 12.5% v|v at day-5. Application into fermentator two liters at temperature 30°C, aeration 1.5 vvm and agitation of 500 rpm showed enzyme production in earlier time (one day fermentation) to achievedenzyme activity of around 1000-1300 U|g protein.
Co-Authors A. Sidik A. Syahril A. T. Karossi S Nana S. Pudjiraharti