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All Journal bionature Agrovigor: Jurnal Agroekoteknologi Sustinere: Journal of Environment and Sustainability Jurnal Layanan Masyarakat (Journal of Public Service) Berkala Penelitian Hayati

Tini Surtiningsih

Department Of Biology, Faculty Of Science And Technology, Universitas Airlangga

Author-ID : 394715

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Energy Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health Social Sciences Transportation Veterinary Other

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Remediation of Pb(II) and Cd(II) in Sea Water by Skeletonema costatum Soedarti, Thin; Surtiningsih, Tini; Hariyanto, Sucipto; Kuncoro, Eko P.
Sustinere: Journal of Environment and Sustainability Vol 1 No 2 (2017): pp. 63 - 143 (December 2017)
Publisher : Centre for Science and Technology, IAIN Surakarta

The heavy metals that polluted sea water can end up polluting fish pond too. Hence, it is necessary to remove the heavy metals before sea water enters the fish pond. The aim of this study was to know the growth, remediation ability, and the highest of removal efficiency of marine diatom Skeletonema costatum in the lead (Pb(II)) and cadmium (Cd(II)) solution. This study used 3 x 4 factorial design, i.e. concentrations of the cell inoculation (5000 cells mL-1; 10000 cells mL-1; and 15000 cells mL-1) and concentrations of Pb(II) and Cd(II) (0; 0.5; 1; and 2 ppm), replicated five times. During five day exposure time, the cell density was observed daily using a microscope (400X), while filtrate of media was analyzed using AAS. The result showed that S. costatum could grow in Pb(II) and Cd(II) at 2 ppm, and could remediate Pb(II) and Cd(II) at 2 ppm. The highest removal efficiency of marine diatom S. costatum in Pb(II) solution was 80.5% (5000 cells mL-1 at 2 ppm on the first day), and in Cd(II) solution was 80% (15000 cells mL-1 at 0.5 ppm on the fifth day).

Pengaruh Paparan Paraquat terhadap Populasi Bakteri yang Berperan sebagai Dekomposer, Pelarut Fosfat, dan Nitrifikasi pada Tanah Perkebunan Desa Batetangnga Sulawesi Barat Sahribulan, Sahribulan; Niâ€™matuzahroh, Niâ€™matuzahroh; Surtiningsih, Tini
bionature Vol 20, No 2 (2019): Oktober
Publisher : Fakultas MIPA UNM

Abstrak. Paraquat adalah herbisida yang digunakan secara luas untuk mengontrol pertumbuhan gulma. Penggunaan paraquat dalam jangka panjang dapat mengganggu keseimbangan ekosistem termasuk mikroorganisme tanah karena keberadaan residunya di dalam tanah. Penelitian ini bertujuan untuk mengevaluasi pengaruh paparan herbisida paraquat terhadap populasi bakteri yang berperan sebagai dekomposer, pelarut fosfat, dan nitrifikasi pada tanah perkebunan. Penelitian ini merupakan penelitian observasional. Pengaruh paparan paraquat terhadap populasi mikroba dievaluasi dari nilai Total Plate Count (CFU/g Tanah). Bakteri yang berperan sebagai dekomposer, pelarut fosfat, dan nitrifikasi pada tanah yang terpapar dan yang tidak terpapar herbisida paraquat. Data penelitian dianalisis secara deskriptif. Hasil penelitian menunjukkan bahwa nilai TPC populasi bakteri yang berperan sebagai dekomposer, pelarut fosfat dan nitrifikasi diantaranya bakteri amilolitik, selulolitik, proteolitik, lipolitik, pelarut fosfat, dan penambat nitrogen pada tanah yang tidak terpapar herbisida paraquat masing-masing sebesar 7.34, 7.04, 7.48, 7.04, 7.77, dan 5.00 (CFU/g). Sedangkan pada tanah yang terpapar herbisida paraquat masing-masing sebesar 6.42, 6.04, 6.25, 6.41, 6.29, dan 4.85 (CFU/g). Kata kunci: paraquat, populasi bakteri, dekomposer, pelarut fosfat, nitrifikasi.

INTERAKSI PSEUDOMONAD PENDARFLUOR INDIGENUS DENGAN Glomus aggregatum TERHADAP SERANGAN PENYAKIT BATANG BERLUBANG DAN PERTUMBUHAN TANAMAN TEMBAKAU Gita Pawana; - Syekhfani; Tini Surtiningsih; Wiwiek Sri Wahyuni
Agrovigor Vol 5, No 2 (2012): SEPTEMBER
Publisher : Universitas Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/agrovigor.v5i2.312

Properties are favorable for the growth of plants from mycorrhizal arbuscular fungi and pseudomonads fluorescenct still need the wider examine, the positive interaction that may be obtained from their association. The purpose of this study was to examine the interaction of Pseudomonas fluorescens Pfim20 with Glomus aggregatum in the rhizosphere of tobacco and evaluate whether the interaction can increase of growth and suppress hollow stalk disease. The method of research is evaluation of antagonistic P. fluorescens Pfim20 towards Pectobacterium carotovorum, followed by green house experiment. The results obtained, the association of P. fluorescens Pfim20 with G. aggregatum positive interaction, on the presence or the absence of association between of P. fluorescens Pfim20 with G. aggregatum there are not level of disease severity and progression of hollow stalk disease, positive interaction between P. fluorescens Pfim20 with G. aggregatum can increase the uptake of phosphate, but can not give a higher biomass.

PELATIHAN PEMBUATAN PUPUK ORGANIK CAIR PADA KELOMPOK TANI DI KABUPATEN PROBOLINGGO Tini Surtiningsih; Fatimah Fatimah; Ni’matuzahroh Ni’matuzahroh; Agus Supriyanto; Tri Nurhariyati
Jurnal Layanan Masyarakat (Journal of Public Services) Vol. 2 No. 1 (2018): Jurnal Layanan Masyarakat
Publisher : Universitas Airlangga

This community service aims to apply the method of making and liquid organic fertilizer and improving the skills of farmers in making liquid organic fertilizer. In addition to reducing the dependence of farmer groups on inorganic fertilizers and utilizing the results of sugarcane waste, namely molasses and microbes as a formula in the manufacture of liquid organic fertilizer. The method that was carried out was to increase insight into liquid organic fertilizer, and how to make it. The results of the socialization of liquid organic fertilizers showed that the insights of farmer groups increased by an average of 77% through the pretest and post test values during socialization. Based on the evaluation shows that the farmer group has been able to make liquid organic fertilizer independently. The socialization of making liquid organic fertilizer provides additional insight for farmer groups on the use of liquid organic fertilizer for agriculture.AbstrakPengabdian kepada masyarakat ini bertujuan untuk menerapkan cara pembuatan dan pupuk organik cair dan meningkatkan keterampilan petani dalam membuat pupuk organik cair. Selain itu untuk mengurangi ketergantungan kelompok tani terhadap pupuk anorganik dan memanfaatkan hasil limbah tebu yaitu molase dan mikroba sebagai formula dalam pembuatan pupuk organik cair. Metode yang dilakukan adalah peningkatan wawasan mengenai pupuk organik cair, dan cara pembuatannya. Hasil sosialisasi pupuk organik cairmenunjukkan bahwa wawasan kelompok tani meningkat rata-rata sebesar 77% melalui nilai pretest dan post test saat sosialisasi. Berdasarkan evaluasi menunjukkan bahwa kelompok tani telah mampu membuat pupuk organik cair secara mandiri. Sosialisasi pembuatan pupuk organik cair memberikan tambahan wawasan bagi kelompok tani terhadap pemanfaatan pupuk organik cair bagi pertanian.

PELATIHAN PENGOLAHAN LIMBAH TOILET MENGGUNAKAN FORMULA MIKROBA DI PERUMAHAN TENGGILIS SURABAYA Fatimah Fatimah; Tini Surtiningsih; Agus Supriyanto; Nima’tuzahroh Nima’tuzahroh; Salamun Salamun; Tri Nurhariyati
Jurnal Layanan Masyarakat (Journal of Public Services) Vol. 2 No. 1 (2018): Jurnal Layanan Masyarakat
Publisher : Universitas Airlangga

Blockage of septic tanks is one of the problems in the community related to the discharge of toilet waste produced. The use of synthetic toilet cleaners that are not environmentally friendly is one of the factors that can inhibit the process of decomposing organic waste. The existence of microbial killer ingredients in toilet cleaning materials that come into the septic tank will actually inhibit the growth of essential microbes that play a role in the perfection of the decomposition process. Lack of understanding of the community related to microbial processes in the processing of toilet waste is one of the factors inability of the community to overcome these problems. This community service aims to disseminate information related to the processing of organic waste, the role of microbes in the processing of toilet waste, how to make microbial formulas that play a role in the processing of toilet waste, as well as efforts to increase the creativity of the community to process their own waste to improve environmental sanitation and improve community welfare. The results of the training showed that the average pretest and post test scores of the cadres of Tenggilis Surabaya village experienced a significant increase in value with a mean value of 37.78 (pretest) to 62.22 (post-test). Toilet waste processing cadres have been able to make biotoilet products that can be used to process limbat toilets in their homes. The informationand insights they receive can be passed on to the community from each cadre.AbstrakPenyumbatan septic tank merupakan salah satu permasalahan di masyarakat terkait dengan buangan limbah toilet yang dihasilkan. Penggunaan pembersih toilet yang berbahan sintetik yang tidak ramah lingkungan merupakan salah satu faktor yang dapat menghambat proses penguraian limbah organik. Keberadaan bahan pembunuh mikroba dalam bahan-bahan pembersih toilet yang ikut masuk ke dalam septik tank justru akan menghambat pertumbuhan mikroba esensial yang berperan dalam kesempurnaan proses dekomposisi. Kurangnya pemahaman masyarakat terkait dengan proses mikroba dalam pengolahan limbah toilet merupakan salah satu faktor ketidakmampuan masyarakat dalam mengatasi permasalahan tersebut. Pengabdian masyarakat ini bertujuanuntuk mensosialisasikan informasi terkait proses pengolahan limbah organik, peran mikroba dalam pengolahan limbah toilet, cara membuat formula mikroba yang berperan dalam pengolahan limbah toilet, serta upaya meningkatkan kreativitas masyarakat untuk mengolah limbahnya sendiri guna meningkatkan sanitasi lingkungan dan meningkatkan kesejahteraan masyarakat. Hasil pelatihan menunjukkan bahwa rerata nilai pretest dan post test kader kelurahan Tenggilis Surabaya mengalami peningkatan nilai yang cukup signifi kan dengan rerata nilai dari 37,78 (pretest) menjadi 62,22 (post test). Kader pengolahan limbah toilet telah mampu membuat produk biotoilet yang dapat dimanfaatkan untuk mengolah limbat toilet di rumah masing-masing. Informasi dan wawasan yang mereka terima dapat di teruskan kepada komunitas asal masing-masing kader.

The potency of Micrococcus sp. L II 61 bacteria as oil sludge cleaning agent Ni’matuzahroh Ni’matuzahroh; Intan Ayu Pratiwi; Tini Surtiningsih; Fatimah Fatimah; Sri Sumarsih
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 22 No 2 (2017): June 2017
Publisher : The East Java Biological Society

This research aimed to reveal the ability of Micrococcus sp. L II 61 bacteria that was isolated from Pegirian Surabaya as oil cleaning agent. This is an experimental research to detect the presence of biosurfactant and lipase enzyme in culture supernatant of Micrococcus sp. L II 61 with aliphatic hy-drocarbon (cooking oil) as a substrate growth. Biosurfactant production was evaluated by measuring the surface tension of supernatant using tensiom-eter du Nouy and measuring the emulsification activity value using diesel oil as hydrocarbon test. Lipase enzyme was detected by measuring lipolitic activity value of crude enzyme (culture supernatant) by using p-nitrofenil palmitic (p-npp) as a substrate test. Data were analyzed descriptively. The results showed that Micrococcus sp. L II 61 produced biosurfactant with surface tension decreasing of culture supernatant up to 30.27 ± 1.17 mN/m compared than aquadest and value of hydrocarbon emulsification activity (AE 1 hour) up to 20.24 ± 0.68 %. Culture of Micrococcus sp. L II 61 after 16 hours incubation have a lipolytic activity 33.53 ± 0.14 U/mL at pH 7 and 37 oC. Supernatant of Micrococcus sp. L II 61 100% (v/v) give the high-est percentage of oil sludge solubility, i.e. 86.38 ± 2.39%. Micrococcus sp. L II 61 is a highly potential to be developed as oil sludge cleaning agent.

EKSPLORASI DAN IDENTIFIKASI BAKTERI SELULOLITIK PADA LIMBAH DADUK TEBU (Saccharum Officinarum L) Evy Ratnasari Ekawati; Ni’matuzahroh Ni’matuzahroh; Tini Surtiningsih; Agus Supriyanto
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 18 No 1 (2012): December 2012
Publisher : The East Java Biological Society

This study aims to determine the diversity of cellulolytic bacteria in sugarcane trash (Saccharum offi cinarum L). This study was an explorative research by observing the clear zone in the medium CMC (carboxyl methyl cellulose) with 0.1% congo red. Characterization of cellulolytic bacteria was through by observing the bacterial of colony morphology, cell shape and physiology characteristics. The research datas were analyzed descriptively. From the results of this study, it were obtained six types of isolates that grew well on CMC medium. Based on the characterization of bacteria, six kinds of these isolates belongs to Cellulomonas sp., Cellvibrio sp., Cytophagasp., Micrococcus sp. and Pseudomonas sp. genera.

BIOFERTILISASI BAKTERI Rhizobium PADA TANAMAN KEDELAI (Glycine Max (L) Merr.) Tini Surtiningsih; Farida; Tri Nurhariyati
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 15 No 1 (2009): December 2009
Publisher : The East Java Biological Society

The aim of this research want to know the influence of the addition Rhizobium bacteria species, dose and combination both of them, on growth and production of soybean plant (Glycine max (L) Merr.). The experimental design of this research was factorial design 4×2, 4 species of Rhizobium are R1 = Rhizobium japonicum, R2 = R. phaseoli, R3 = R. leguminosarum, R4 = mixture of R1, R2 and R3, and 2 dose of inoculan Rhizobium (D1 = 5 m/plant, and D2 = 10 ml/plant) with 1010 sel bacteria/ml and 5 replications. Independent variable is species of Rhizobium, dose of inoculan Rhizobium and combination both of them. Dependent variable is dry matter, weight of nodules and dry weight of seeds. The harvest data was analyzed by Kruskal-Wallis Test using 5% level (a = 0.05) followed by Mann- Whitney Test. The result of this research show that species of Rhizobium, dose of inoculan Rhizobium and combination both of them present insignificant result (a > 0.05) on soybean growth and production, but the mixture of Rhizobium species with high level dose of bacteria, present better result than single species with low dose of bacteria.

BIODEGRADASI MINYAK OLEH Rhodotorula dan Candida HASIL ISOLASI DARI PELABUHAN TANJUNG PERAK SURABAYA Tri Nurhariyati; Ni’matuzahroh Ni’matuzahroh; Tini Surtiningsih
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 12 No 1 (2006): December 2006
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/394

A research about isolation and capability of isolat yeast from Tanjung Perak Harbor, Surabaya in degrading kerosen, gas oil ,and lubricant were conducted. This research were done to know the influence of Rhodotorula, Candida and Mix of Rhodotorula and Candida; the influence of oil type and interaction of both (between yeast and oil) in decreasing oil weight. Research design was laboratory experimental and using the factorial 4 × 3 with five replication. The data were analyzed by two-way ANOVA (p: 0.05) and followed by t test. The result of the biodegradation test showed that the kind of yeast, the kind of oil weight and interaction of both influence in decreasing of oil weight. The most decreasing of oil weight obtained by mix of Rhodotorula and Candida (82.55%), kerosen (66.59%) and combination mix of yeast (Rhodotorula and Candida) and kerosen (89.32%).

KEANEKARAGAMAN KHAMIR PENDEGRADASI MINYAK HASIL ISOLASI DARI PELABUHAN TANJUNG PERAK SURABAYA Tri Nurhariyati; Ni’matuzahroh Ni’matuzahroh; Tini Surtiningsih
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 9 No 2 (2004): June 2004
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/427

The aims of this research was to obtain diversity of hydrocarbonoclastic yeast isolated from Tanjung perak harbor Surabaya. Exploration of yeast was conducted by isolation and identification of isolated yeast. Identification of yeast based on characteristics of colonies, cell shape, and biochemical tests. From this research, it was obtained 9 hydrocarbonoclastic yeasts. They were 8 generas: Rhodotorula, Candida, Geotrichum, Torulopsis, Trichosporon, Cryptococcus, Debaryomyces, and Saccharomyces.

Co-Authors - Syekhfani Agus Supriyanto Evy Ratnasari Ekawati Farida Fatimah Fatimah Gita Pawana Intan Ayu Pratiwi Kuncoro, Eko P. Niâ€™matuzahroh, Niâ€™matuzahroh Nima’tuzahroh Nima’tuzahroh Ni’matuzahroh Ni’matuzahroh Ni’matuzahroh Ni’matuzahroh Ni’matuzahroh Ni’matuzahroh Ni’matuzahroh Ni’matuzahroh Ni’matuzahroh Ni’matuzahroh Sahribulan, Sahribulan Salamun Salamun Sri Sumarsih Sucipto Hariyanto Thin Soedarti Tri Nurhariyati WIWIEK SRI WAHYUNI

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