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All Journal Medical Journal of Indonesia JFIOnline Jurnal Aisyah : Jurnal Ilmu Kesehatan Pharmaceutical Sciences and Research (PSR)
Herman Suryadi
Departemen Farmasi FMIPA UI
Health Professions Medicine & Pharmacology Nursing Public Health Veterinary

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Quantitative analysis of lactose and lactulose in urine by high performance liquid chromatography for determination of intestinal lactase activity Dharmasetiawani, Nani; Suryadi, Herman; Firmansyah, Agus
Medical Journal of Indonesia Vol 12, No 1 (2003): January-March
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (231.365 KB) | DOI: 10.13181/mji.v12i1.84

Abstract

Determination of intestinal lactase activity is directly done by measuring its activity in intestinal epithelium. This is an invasive method and ethically can not be done in healthy infants. Indirectly, determination of lactase activity, stated as excretion and ingestion ratio of lactose and lactulose, needs 30 hours hospitalized infants. The aim of this study was to look for a method for determination of lactase activity which is not invasive and not necessary hospitalized. Using this method  lactose and lactulose were given as a single oral load after 2 hours fasting. Urine were collected for 5 hours starting from consuming sugar solution and then lactose and lactulose concentration in the urine were measured by High Performance Liquid Chromatography. The results showed single oral load of lactose and lactulose can be used for determination of lactase activity in infant and the infants were observed only for 7 hours. (Med J Indones 2003; 12: 8-12) Keywords: Lactase activity, Lactulose, Lactose, High Performance Liquid Chromatography
OPTIMASI DAN VALIDASI METODE ANALISIS ASAM NIKOTINAT SERTA STABILITAS INOSITOL HEKSANIKOTINAT Harahap, Yahdiana; Suryadi, Herman; Wardatun, Sri
JFIOnline | Print ISSN 1412-1107 | e-ISSN 2355-696X Vol 6, No 1 (2012)
Publisher : Indonesian Research Gateway

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Abstract

Nicotinic acid is a therapeutic agent for treatment atherosclerosis. Inositol hexanicotinate isan agent that can be hydrolyzed with release nicotinic acid. The low level of free nicotinicacid from inositol hexanicotinate in blood, it’s the reason why it needs method analysis withhigh sensitive and selective. The aims of this research were to optimize and validationmethod analysis nicotinic acid and stability study of inositol hexanicotinate by highperformance liquid chromatography. The method was optimated with variation compositionmobile phase, variation flow rate and optimation process exctraction. Condition analysiswere optimum with use a Kromasil column (250 mm x 4,6 mm) RP, mobile phase mixeddipotassium hydrogen phosphate and potassium dihydrogen phosphate 10 mM containingtetrabuthylammonium bromide 5 mM pH 7 with acetonitril (100:9), flow rate 0,8 ml/minute,with internal standard coffein in 263 nm wave lenght. The standard curve was linear over aconcentration range 124,84 to 5000 ng/ml of nicotinic acid in plasma. The HPLC method wasvalidated with accuracy -6,8779 to 3,09 %, precision 0,3 to 3,71 % and recovery 93,12 -103,09 %. The results of a stability study indicated that inositol hexanicotinate was unstablein plasma samples, but was stable in 0,6 M perchloric acid for to 24 hour at 40C. ABSTRAKAsam nikotinat merupakan obat yang dapat digunakan untuk ateroskerosis dan inositolheksanikotinat merupakan senyawa yang dapat terhidrolisis melepaskan asam nikotinat.Penelitian ini bertujuan untuk optimasi dan validasi metode analisis asam nikotinat, sertamenentukan stabilitas inositol heksanikotinat menggunakan kromatografi cair kinerja tinggi.Kondisi analisis yang optimum diperoleh dengan menggunakan kolom Kromasil (250 mm x4,6 mm) RP, fase gerak campuran kalium dihidrogen fosfat dan dikalium hidrogen fosfat 10mM yang mengandung tetrabutilammonium bromida 5 mM pH 7 dengan asetonitril (100:9),kecepatan alir 0,8 ml/menit, dengan baku dalam kafein, yang dideteksi pada panjanggelombang 263 nm. Kurva kalibrasi linier dari 124,84 sampai 5000 ng/ml. Hasil validasimetode menunjukkan akurasi -6,87 hingga 3,09 %, presisi 0,3 hingga 3,71 % dan perolehankembali 93,12 hingga 103,09%. Hasil uji stabilitas menunjukkan bahwa inositolheksanikotinat tidak stabil dalam plasma tetapi stabil dalam asam perklorat 0,6 M padapenyimpanan 40C selama 24 jam.
OPTIMASI DAN VALIDASI METODE ANALISIS ASAM NIKOTINAT SERTA STABILITAS INOSITOL HEKSANIKOTINAT Harahap, Yahdiana; Suryadi, Herman; Wardatun, Sri
Jurnal Farmasi Indonesia Vol 6, No 1 (2012)
Publisher : Jurnal Farmasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35617/jfi.v6i1.94

Abstract

Nicotinic acid is a therapeutic agent for treatment atherosclerosis. Inositol hexanicotinate isan agent that can be hydrolyzed with release nicotinic acid. The low level of free nicotinicacid from inositol hexanicotinate in blood, itâ??s the reason why it needs method analysis withhigh sensitive and selective. The aims of this research were to optimize and validationmethod analysis nicotinic acid and stability study of inositol hexanicotinate by highperformance liquid chromatography. The method was optimated with variation compositionmobile phase, variation flow rate and optimation process exctraction. Condition analysiswere optimum with use a Kromasil column (250 mm x 4,6 mm) RP, mobile phase mixeddipotassium hydrogen phosphate and potassium dihydrogen phosphate 10 mM containingtetrabuthylammonium bromide 5 mM pH 7 with acetonitril (100:9), flow rate 0,8 ml/minute,with internal standard coffein in 263 nm wave lenght. The standard curve was linear over aconcentration range 124,84 to 5000 ng/ml of nicotinic acid in plasma. The HPLC method wasvalidated with accuracy -6,8779 to 3,09 %, precision 0,3 to 3,71 % and recovery 93,12 -103,09 %. The results of a stability study indicated that inositol hexanicotinate was unstablein plasma samples, but was stable in 0,6 M perchloric acid for to 24 hour at 40C. ABSTRAKAsam nikotinat merupakan obat yang dapat digunakan untuk ateroskerosis dan inositolheksanikotinat merupakan senyawa yang dapat terhidrolisis melepaskan asam nikotinat.Penelitian ini bertujuan untuk optimasi dan validasi metode analisis asam nikotinat, sertamenentukan stabilitas inositol heksanikotinat menggunakan kromatografi cair kinerja tinggi.Kondisi analisis yang optimum diperoleh dengan menggunakan kolom Kromasil (250 mm x4,6 mm) RP, fase gerak campuran kalium dihidrogen fosfat dan dikalium hidrogen fosfat 10mM yang mengandung tetrabutilammonium bromida 5 mM pH 7 dengan asetonitril (100:9),kecepatan alir 0,8 ml/menit, dengan baku dalam kafein, yang dideteksi pada panjanggelombang 263 nm. Kurva kalibrasi linier dari 124,84 sampai 5000 ng/ml. Hasil validasimetode menunjukkan akurasi -6,87 hingga 3,09 %, presisi 0,3 hingga 3,71 % dan perolehankembali 93,12 hingga 103,09%. Hasil uji stabilitas menunjukkan bahwa inositolheksanikotinat tidak stabil dalam plasma tetapi stabil dalam asam perklorat 0,6 M padapenyimpanan 40C selama 24 jam.
Uji Efektivitas Antimikroba Beberapa Merek Dagang Pembersih Tangan Antiseptik Radji, Maksum; Suryadi, Herman; Ariyanti, Dessy
Majalah Ilmu Kefarmasian
Publisher : UI Scholars Hub

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Abstract

In Modern life, peoples are more conscious to keep their healthy. One of the way to keep our body healthy is by keeping our hand clean. Washing hands with soap and water, sometimes is unpractical since it need water to rinse off the soap. Prod-uct innovation has been developed to overcome the unpractical side, which is called antiseptic hand wash or hand sanitizer. This study has been done to evaluate the antimicrobial activity of some hand sanitizer products marketed in Jakarta. The re-sult showed that alcohol-based hand sanitizer and triclosan-based hand sanitizer used in this experiment were significantly reduce bacterial counts on hands, but not all of the samples are effective in reducing fungal counts on hands.
Pemeriksaan Bakteriologis Air Minum Isi Ulang Di Beberapa Depo Air Minum Isi Ulang Di Daerah Lenteng Agung dan Srengseng Sawah Jakarta Selatan Radji, Maksum; Oktavia, Heria; Suryadi, Herman
Majalah Ilmu Kefarmasian
Publisher : UI Scholars Hub

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Abstract

Recently, refilled drinking water stores are flourishing in the some cities of Indonesia. This research tries to find out the quality of refilled drinking water at some shop in Jagakarsa, South Jakarta. The samples of refilled drinking water were taken from thir-teen shops around Lenteng Agung and Srengseng Sawah, Jagakarsa area. The bacte-riological test of refilled drinking water was to detect the availability of Coliform bacteria and identification of some bacterial pathogens such as Escherichia coli, Sal-monella, Staphylococcus aureus, Clostridium perfringens and Pseudomonas aeruginosa. It had been concluded that two of thirteen samples of refilled drinking water had the total number of bacteria above of the limit number according to the standard about the quality and requirement of drinking water. Four of the thirteen samples contain Sta-phylococcus aureus and none of the samples contain Escherichia coli, Salmonella, Clostridium perfringens and Pseudomonas aeruginosa.
Purifikasi Inhibitor Atpase/Rna Helikase Virus Japanese Encephalitis Dari Streptomyces chartreusis Elfita, Lina; Ratnakomala, Shanti; Suryadi, Herman; Lisdiyanti, Puspita; Utama, Andi
Majalah Ilmu Kefarmasian Vol. 6, No. 2
Publisher : UI Scholars Hub

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Abstract

Japanese encephalitis virus (JEV) is a neuropathogenic virus commonly caused cen-tral nervous diseases such as meningitis and severe encephalitis. Although vaccine has been developed, no specific and effective drug is available so far. We previously carried out a screening of inhibitor of JEV RNA helicase, an enzyme that essential for virus replication, from Actinomycetes and found that Streptomyces chartreusis produce the inhibitor of JEV RNA helicase. In this study, an extracellular protein which has inhibition activity on ATPase activity of JEV RNA helicase was purified from supernatant of Streptomyces chartreusis culture by ammonium sulfate pre-cipitation and size exclusion chromatography. SDS-PAGE analysis showed a single band with aproximate molecular mass of 11,4 kDa, suggesting that the inhibitor was successfully purified into a single protein.
Amobilisasi Sel Lactobacillus Acidophilus FNCC116 Untuk Demineralisasi Limbah Kulit Udang Dalam Pengolahan Kitin Betha, Ofa Suzanti; Setyahadi, Siswa; Suryadi, Herman
Majalah Ilmu Kefarmasian
Publisher : UI Scholars Hub

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Abstract

Chitin, a homopolimer, is the most abundant renewable natural resources after cellulose. Chitin and its derivatives hold many applications in agriculture, textile, pharmacy and medic. Chitin that extracted from waste shrimp shells by biological fermentation has better quality than chemical procees. Demineralization of chitin by biological procees use lactic acid as product of fermentation. Deproteinization of chitin use proteolytic activity of enzyme that produce by bacteria in fermentation. Lacto-bacillus acidophilus FNCC116 has been immobilized by entrapment methods and 2% sodium alginate in 0,2 M CaCl2 as the matric . The ability of immobilized Lacto-bacillus acidophilus FNCC116 cell in fermentation was tested. The fermentation that was carried out in medium which consist of 6% glukosa, 1,5% yeast extract, 0,003% MnSO4 0,003% FeSO4.7H2O, 0,02% MgSO4.7H2O and has been producted 2,24% lactic acid. Demineralization of waste shrimp shell with 30% immobilized Lactobacillus acidophilus FNCC116 cell has successfully decreased ash content tol 1,18% and produced lactic acid maximum 2.24%. Immobilization of Lactobacillus acidophilus FNCC116 cell promised an efficient method in bioproceesing of chitin recovery.
Analisis Formalin dalam Sampel Ikan dan Udang Segar dari Pasar Muara Angke Suryadi, Herman; Kurniadi, Maryati; Melanie, Yuanki
Majalah Ilmu Kefarmasian Vol. 7, No. 3
Publisher : UI Scholars Hub

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Abstract

The use of formalin as a food additive has been prohibited by the ministry of health as stipulated in The Indonesia Ministry of Health Regulations No.722/Menkes/Per/IV/88. However, in recent years the Indonesian authorities have found trace amounts of formalin as a preservative in perishable foods such as fish and shrimp. The aim of this research is to identify the use of formalin in fresh fish and shrimp samples sold in Muara Angke Market as the fresh seafood market in Jakarta. The first step of this research was formalin identification in fish and shrimp samples and continued by quantitative analysis to assure the results obtained. Qualitative determination of formalin was carried out by Schryver reagent and the quantitative determination was carried out pectrophotometrically using Nash reagent. Validation of UV-Vis spectrophotometric method for determination of formalin showed that Nash reagent was suitable to determine formalin. The limit of detection, limit of quantitation, and coefficient of variation for formalin were 0,0102 mg/L, 0,0341 mg/L, and 0,09%, respectively. Recovery of formalin in fish samples was 89,79-109,58% and shrimp samples was 82,11-97,76%. Qualitative determination in six fish samples and six shrimp samples showed negative results and the quantitative analysis confirmed that formalin was not found in the fresh fish and shrimp samples from Muara Angke Market.
Analisis Kandungan Ion Flourida pada Sampel Air Tanah dan Air PAM Secara Spektrofotometri Astriningrum, Yodifta; Suryadi, Herman; Azizahwati, Azizahwati
Majalah Ilmu Kefarmasian Vol. 7, No. 3
Publisher : UI Scholars Hub

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Abstract

Fluoride ion is one of the compounds that are known to have benefits in the prevention of dental caries when used in certain concentrations, but also hasnegative effects that may cause the occurrence of dental and bone fluorosis when the intake was in excessive concentration. One of the fluoride intakes comes fromwater that is consumed. The aim of this research was to identify and measurefluoride ion levels in groundwater and piped water that used as drinking water consumption in the community. Measurement of fluoride ion concentration isdone by using visible spectrophotometry at the maximum wavelength of 586 nmusing the sodium 2-parasulfophenylazo 1,8-dihydroxy-naphthalene-3,6disulfonate (SPADNS)-zirconil acid reagent. This method was optimized by thesearch of range of absorption which stable for 10 minutes after reagent addition.The limit of detection, limit of quantitation, and coefficient of variation forfluoride ion were 0.0452 mg/L, 0.1506 mg/L, and 0,63%, respectively. While therecovery of fluoride ion in sample were in the range of 90,50-102,04%. The measurement results of the samples showed levels of fluoride ions in groundwaterand piped water varied between 0.05 to 0.78 mg/L. This range was still within allowed levels according the rules of Indonesian health ministers No.492/MENKES/PER/IV/2010 where the maximum allowable fluoride concentration is 1.5 mg/L.
Analisis Kandungan Ion Fluorida pada Sampel Air Tanah dan Air PAM Secara Spektrofotometri Astriningrum, Yodifta; Suryadi, Herman
Majalah Ilmu Kefarmasian Vol. 8, No. 2
Publisher : UI Scholars Hub

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Abstract

Fluoride ion is one of the compounds that are known to have benefits in the prevention of dental caries when used in certain concentrations, but also hasnegative effects that may cause the occurrence of dental and bone fluorosis when the intake was in excessive concentration. One of the fluoride intakes comes fromwater that is consumed. The aim of this research was to identify and measurefluoride ion levels in groundwater and piped water that used as drinking water consumption in the community. Measurement of fluoride ion concentration isdone by using visible spectrophotometry at the maximum wavelength of 586 nm using the sodium 2-parasulfophenylazo 1,8-dihydroxy-naphthalene-3,6disulfonate (SPADNS)-zirconil acid reagent. This method was optimized by thesearch of range of absorption which stable for 10 minutes after reagent addition.The limit of detection, limit of quantitation, and coefficient of variation forfluoride ion were 0.0452 mg/L, 0.1506 mg/L, and 0,63%, respectively. While therecovery of fluoride ion in sample were in the range of 90,50-102,04%. The measurement results of the samples showed levels of fluoride ions in groundwaterand piped water varied between 0.05 to 0.78 mg/L. This range was still within allowed levels according the rules of Indonesian health ministers No.492/MENKES/PER/IV/2010 where the maximum allowable fluoride concentration is 1.5mg/L.
Co-Authors . Harmita . Hayun Agus Firmansyah ANDI UTAMA Arif Arrahman, Arif Arifianti, Ayun Erwina Azizahwati Azizahwati, Azizahwati Berna Elya Chairunissa, Ananda Hanny Dessy Ariyanti Forestrania, Roshamur Cahyan Fready SIP Hayun Hayun, Hayun Heria Oktavia, Heria Irianti, Marina Ika Jantan, Ibrahim Lina Elfita Luh Putu Ratna Sundari Makau, Juliann Nzembi Maksum Radji Maryati Kurniadi Melanie, Yuanki Mun'im, Abdul Nani Dharmasetiawani Ofa Suzanti Betha PUSPITA LISDIYANTI Rahmasari, Ratika SHANTI RATNAKOMALA Siswa Setyahadi Sri Wardatun Syarifah, Andiri Niza Yahdiana Harahap Yodifta Astriningrum, Yodifta