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All Journal AQUATIC SCIENCE & MANAGEMENT Jurnal MIPA PHARMACON CHEMISTRY PROGRESS Jurnal Penelitian Hutan Tanaman Jurnal Ilmu dan Teknologi Pangan
Edi Suryanto
Jurusan Kimia Fak. MIPA Unsrat Manado
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Decision Sciences, Operations Research & Management Mathematics Medicine & Pharmacology Physics

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EFIKASI Bacillus thuringiensis TERHADAP HAMAULAT DAUN GAHARU Heortia vitessoides Lestari, Fajar; Suryanto, Edi
Jurnal Penelitian Hutan Tanaman Vol 9, No 4 (2012): JURNAL PENELITIAN HUTAN TANAMAN
Publisher : Pusat Penelitian dan Pengembangan Peningkatan Produktivitas Hutan

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Abstract

ABSTRACTHeortia vitessoides worms attack gaharu plants in Kandangan, Regency of Hulu Sungai Selatan and Barabai, Regency of Hulu Sungai Tengah, Central Kalimantan province. This pest attack causes stunted growth of the gaharu plants. The objective of this study was to determine the efficacy of Bacillus thuringiensis against the worm pests H. vitessoides. The study was conducted in the protected forest laboratory of the Forest Research Institution of Banjarbaru and in the region of gaharu stands in Kandangan and Barabai, South Kalimantan. The research results showed that the insecticide with the active substance B. thuringiensis at a concentration of 0.5 g/l and 1.5gr/l effectively caused larval mortality of 100% on day 3, and the treatment with the concentration of 1 g/l and 2 g/l effectively caused 100% mortality of larvae on day 2 at the laboratory scale. At the field scale all concentrations resulted in 100% larval mortality at day 3. Variations of concentrations used did not significantly affect mortality of larvae in the laboratory and on the field.ABSTRAKUlat Heortia vitessoides menyerang tanaman gaharu di Kandangan Kabupaten Hulu Sungai Selatan dan Barabai Kabupaten Hulu Sungai Tengah Provinsi Kalimantan Selatan. Serangan hama ini menyebabkan terjadinya gangguan pertumbuhan pada tanaman gaharu. Tujuan penelitian ini untuk mengetahui efikasi Bacillus thuringiensis terhadap hama ulat H. vitessoides.   Penelitian dilakukan di laboratorium perlindungan hutan Balai Penelitian Kehutanan Banjarbaru dan areal tegakan gaharu di Kandangan dan Barabai, Kalimantan Selatan. Hasil penelitian menunjukkan bahwa insektisida berbahan aktif B. thuringiensis pada konsentrasi 0,5 gr/l dan 1,5gr/l efektif menyebabkan mortalitas larva sebesar 100% pada hari ke-3, perlakuan dan konsentrasi 1 gr/l dan 2 gr/l efektif menyebabkan mortalitas larva sebesar 100% pada hari ke-2 pada skala laboratorium. Skala lapangan menunjukkan bahwa semua konsentrasi menyebabkan mortalitas larva 100% pada hari ke-3, sementara variasi konsentrasi yang digunakan tidak berpengaruh nyata terhadap mortalitas larva pada skala laboratorium dan skala lapangan.
AKTIVITAS ANTIOKSIDAN DAN ANTIFOOKSIDASI DARI EKSTRAK BUAH LABU KUNING (Cucurbita Moschata) [Antioksidan Activities And Antifotooxidation of Pumpkin (Cuurbita Moschata) Extracts] Gumolung, Dokri; Suryanto, Edi; Mamuaja, Christin
Jurnal Ilmu dan Teknologi Pangan Vol 1, No 1 (2013)
Publisher : Pascasarjana Unsrat

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The objective of this study were to identify carotenoid, phenolic contents, and to determine the free radical scavenging activiy, and active oxygen species on the skin, flesh, flakes and seeds of pumpkin.Active oxygen compound quenching effects was conducted using linoleic acid as substrate containing erythrosine as a sensitizer and exposed under continuous illumination (400 lux) with the fluorescent light for up to 15 hours. The results showed that all of the pumpkin parts containing beta-carotene and phenolic. All parts of the fruit had a free radical scavenging activity and activity oxygen species.Scavenging free radical activity of EET-Skin 78.85%, EET-Meat 62.16%, EET-tuft 74.74% and 78.28% EET-seed respectively were observed in this study. In the measurement of oxygen singlet activity stabilizer in the photooxidation of linoleic acid system for 15 hours, the amount of extract concentration significantly contribute to reduce the oxidation of linoleic fatty acid. The extract seeds from pumpkin was the most strongly inhibit the oxidation of linoleic fatty acid in the photooxidation. It can be concluded that pumpkin seeds extract had the most strongest free radical scavenging activity and active oxygen species.
Extraction of Pectin from Cui Lemon (Citrus microcarpa) and Its Application in Pineapple Jam Rompas, Veckie Frankie; Mamuaja, Christine F.; Suryanto, Edi
Jurnal Ilmu dan Teknologi Pangan Vol 4, No 2 (2016)
Publisher : Pascasarjana Unsrat

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Cui lemon (Citrus microcarpa Bunge) is a type of small citrus variety that is widely available in North Sulawesi. It is very popular as a preservative and deodorizing agent of fishy odor in fish products, and it is used in traditional chilly sauce "Dabu-Dabu" with a very distinctive aroma arouse your appetite. Lemon cui contains vitamin C and phenolic compounds. Lemon is an important economic value and is rich in antioxidant phytochemicals particularly high content of vitamin C. The objectives of this study were to examine the effect of temperature, and extration time on the pectin yield, and examine the effect of lemon pectin addition on panelist preference of pineapple jam. The pectin in the sample was extracted at different temperatures, namely  70, 80, 90 and 100 0C with 60 and 80 minutes extraction time, then the pectin was applied in the processing of pineapple jam.  The pectin extraction was carried out in the following procedures. The experiment was arranged in completely randomized design (CRD) in factorial experiment.  The jam products were calculated and analyzed forAnalysis conducted for pectin yield, methoxyl, moisture, and ash contents.  The data by were analyzed using analysis of variance (ANOVA) and followed by LSD test if the anova was significant. The results showed that temperature affected yield of extraction of pectin  significantly (P<0.05) whereas time had no effect (P>0.05). Addition of 1% pectin in pineapple jam significantly affected (P<0.05) the aroma and texture,but were not for color and the taste (P>0.05). The panelist test on pineapple jam showed that, in average, the panelists liked the addition of pectin treatment for parameter of aroma and texture, but for the color and taste parameters panelists give a neutral assessment. Extraction at a temperature of 100 0C for 80 minutes resulted in optimal pectin yield (3.74%) with methoxyl, moisture, and ash contents of 12.66%, 10.37%, 1.63%, respectively.%. Keywords: lemon cui, extraction, pectin, methoxyl, pineapple jams
KANDUNGAN FENOLIK DAN AKTIFIVATS ANTIOKSIDAN EKSTRAK KULIT ARI KENARI (Canarium vulgare Sp.) Djasibani, Hemy R.; Djarkasi, G.S. Suhartati; Suryanto, Edi
Jurnal Ilmu dan Teknologi Pangan Vol 1, No 1 (2013)
Publisher : Pascasarjana Unsrat

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Canary is original plants Indonesia which growing many in Indonesia area part of east, like North Sulawesi, Maluku, Seram and Alor island. This study aims to determine the total conten of phenolic compounds and antioxidant activity of extracts of canary husk of some species of Canarium vulgare Sp. solvent is hexane, ethanol, ethyl acetate and acetone. Results showed that the canary husk extract of Canarium vulgare Sp. species has a total phenolic content and antioxidant activity are great. Highest total phenolic content of the extract produced by solvent acetone was 171.0 mg/kg, followed by extracts of ethyl acetate, ethanol, and hexane has a total phenolic content of consecutive 111,17 mgkg, 99,67 mg/kg, and 67,17 mg/kg and the highest antioxidant activity in the solvent ethyl acetate extract of 93.66% followed by 92.97% acetone extract, ethanol amounted to 89.92% and by 14.45% hexane. Result of this research indicate that canary husk acetone extract have fenolik content and highest antioxidant.
Chemical Characteristics and Antioxidant Activity of Instant Drink Lemon Kalamansi (Citrus microcarpa) with Addition of Clove Leaf (Eugenia carryophyllus) and Nutmeg Meat (Myristica fragrans) Extracts Edam, Mariati; Suryanto, Edi; Djarkasi, Gregoria S. S.
Jurnal Ilmu dan Teknologi Pangan Vol 4, No 1 (2016)
Publisher : Pascasarjana Unsrat

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Lemon kalamansi, clove leaf and meat nutmeg have bioactive compounds that have potential as antioxidants so it can serve as raw material for made of instant drink. This study aims to analyze the chemical characteristics and antioxidant activity of instant drink lemon kalamansi with the addition of clove leaf and meat nutmeg extract. The research method is experimental research with a completely randomized design as many as three levels of treatment with three replications arranged in the form of formulations. The results showed that the instant drink lemon kalamansi characteristics containing antioxidant such as phenolic compounds which range from 24,50-130,51 µg/mL (highest in formulation B is the addition of clove leaf extract 20%), vitamin C ranged 278,70-390,55 mg/100 g bahan  (highest in formulation B is 390,55 mg/100 g bahan), as well as the activity of antioxidant free-radical scavengers ranged 18,11-46,73% (formulation B, namely the addition 20% of clove leaf extract showed the highest activity in counteract free radicals). Based on the results of this study it can be concluded that the instant drink lemon kalamansi with the addition of meat nutmeg and clove leaf extract contains antioxidant compounds that have potential as functional drinks. Keywords: antioxidant, clove leaf, instant drink, kalamansi, nutmeg meat
Physicochemical Characteristics and Antioxidant Activity of Rice Analog from Baruk Sago (Arenga microcarpha) and Purple Sweet Potatoes (Ipomea batatas L. Poiret) Kaemba, Almawaty; Suryanto, Edi; Mamuaja, Christine F.
Jurnal Ilmu dan Teknologi Pangan Vol 5, No 1 (2017)
Publisher : Pascasarjana Unsrat

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Rice analog is an imitation of rice which made ingredients such as tubers and cereals that are similar in shape or nutrition composition as rice. The aim of this research was to physicochemical characteristic of rice analog from baruk sago and purple sweet potatoes, and to analyze antioxidant compound and its activity. The physical characteristic which evaluated as the color using color analyzer, although chemical characteristic includes moisture content, ash content, fat content, protein, fiber and carbohydrate using AOAC method. An antioxidant compound which evaluated was anthocyanin, and antioxidant activity was total antioxidant which evaluated using spectrophotometry. The result showed that rice analog which possesses concentration 65% was the best favorite level having good, aroma, taste and excelent texture using hedonic scales. Physical characteristic of rice analog color exhibit L 62,01, a* 12,49 dan b* 1,54. Rice analogue possesses chemical characteristic moisture content 7,99%, ash content 0,94%, fat content 0,81%, protein content 0,092%, fiber content 2,89%, and carbohydrate content 87,28%. Rice analogue possess anthocyanin 8,68 mg/L and exhibit total antioxidant 31,52 mg/mL. Physical characteristic of color from analog rice was less bright, and chemical characteristic was close to rice. Analog rice possess antioxidant compounds and show antioxidant activity. Keywords: anthocyanin, baruk sago, physicochemical, purple sweet potatoes, rice analog, total antioxidant
The yield, nitrogen content, and dye’s binding capacity of chitin and chitosan of rotifer Brachionus rotundiformis Modaso, Riny; Suryanto, Edi; Tallei, Trina; Rumengan, Inneke F.M
AQUATIC SCIENCE & MANAGEMENT Edisi Khusus 1 (2013): Mei
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jasm.0.0.2013.2286

Abstract

Chitin and chitosan from rotifer have not been previously explored due to the problem of high biomass required for extraction. This study aimed to obtain the chitin yield from rotifer biomass produced in mass culture, and to characterize the basic properties of chitin and chitosan, especially, nitrogen content and dye binding capacity. Methods of extraction and deacetylation of chitin were adopted from Chandumpai et al. (2004) with modification. The nitrogen content was analyzed using the semi-micro Kjeldahl method (AOAC, 1984), and dye binding capacity using the method developed by Cho et al. (1998). Results show that the yield of chitin obtained from the rotifer sample was relatively small (4.64%), and the yield of chitosan was even smaller, only 2,62%. The proportion of chitosan over chitin was 52,4%. The nitrogen content of chitin and chitosan of rotifer were 4.23 to 4.36% and 7.12-7.23%, respectively. The capacity of chitin to maintain the bonded dye was relatively stronger than that of chitosan, but the chitosan had higher capacity to absorb the dye. The characterization of other important properties of chitin and chitosan to be developed as biopolymer for industry is further aspects to be assessed© Eksplorasi kitin dan kitosan dari rotifer belum pernah dilakukan sebelumnya karena dihadapkan pada permasalahan kebutuhkan biomassa yang banyak. Penelitian ini bertujuan untuk memperoleh rendemen kitin dari biomassa rotifer hasil kultur massal, dan menjajaki karakterisasi awal sifat fisika kimia kitin yang mendasar yaitu daya ikat zat warna dan kadar nitrogen. Ekstraksi kitin dan kitosan dilakukan berdasarkan metode Chandupai et al. (2004), kadar nitrogen menggunakan metode semi-micro Kjeldahl (AOAC, 1984), dan daya ikat zar warna ditentukan menurut metode Cho et al. (1998). Dari penelitian ini, rendemen kitin yang diperoleh dari hasil ekstraksi relatif kecil berkisar 4,64 %, dan rendemen kitosan bahkan lebih kecil lagi hanya 2,62%. Proporsi kitosan dari kitin yaitu 52.4 %. Kadar nitrogen pada kitin rotifer berkisar 4,23-4,36 % dan kitosan berkisar dari 7,12-7,23%. Kitin memiliki sifat yang lebih baik dalam ketahanan untuk mengikat zat warna sebaliknya kitosan memperlihatkan sifatnya yang dapat mengikat zat warna dalam tingkat adsorpsi yang tinggi dibanding kitin. Karakterisasi  sifat fisika kimia kitin dan kitosan yang penting lainnya untuk pengembangannya sebagai biopolimer dalam skala industri, merupakan aspek-aspek yang perlu dikaji lebih lanjut©
AKTIVITAS ANTIOKSIDAN DARI EKSTRAK KULIT NANAS (ANANAS COMOSUS (L) MERR) Hatam, Sri Febriani; Suryanto, Edi; Abidjulu, Jemmy
PHARMACON Vol 2, No 1 (2013): pharmacon
Publisher : PHARMACON

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ABSTRACT The objectives of this research were to determine antioxidant n activity from pineapple peal extract. Pineapple peel extracted by maceration, soxhlet and reflux using ethanol 80% as a solvent. Phenolics and flavonoids content of Crude extract were analyzed.Evaluation of antioxidant activity using DPPH free radicals method, The results shows that maceration, soxhlet and reflux extract possess phenolics content 16,53; 28,78; 16,02 g/mL respectively, and  flavonoid total content 3,514; 5,115; 4,414 g/mL respectively. Maceration extract with concentration 3000 g/mL possess low free radical scavenging activity, and soxhlet extract possess the highest.Inhibition concentration 50% (IC50) of soxhlet extract were 602,56 g/mL, it?s indicate that soxhlet extract possess high scavenging of 50% free radicals. This result shows that pineapple peel extract possess phytochemical phenolics and flavonoids that potential to be antioxidant and as active compound of sun screen. Keywords: antioxidant, pineapple peel extract.
POTENSI ANTIOKSIDAN FENOLIK DARI FAMILI MYRTACEAE DAN PERANNYA SEBAGAI BAHAN AKTIF TABIR SURYA Suryanto, Edi
CHEMISTRY PROGRESS Vol 3, No 2 (2010)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/cp.3.2.2010.18983

Abstract

ABSTRACTSuryanto et al., 2010. The potential of phenolic antioxidant from Myrtaceae family as sunscreen active compound.The objectives of study was to determine free radical scavenging activity and measure sun protective activity of the phenolic extract in Myrtaceae family in vitro. Analyses of phytochemicals were based on total phenolic, flavonoid and condensed tannins. The antioxidant activities of extracts were determined by free radical 1.1-diphenyl-2-picrylhydrazyl (DPPH) assay. Syzygium polyanthum, clove and Eugenia aquea powder was extracted with methanol 80% at room temperature for 24 hours. Free radical scavenging activity of each extracts evaluated at 100-200 ppm level. The higher total phenolic and tannin content was find clove leave extract, followed by Eugenia aquea leave extract and Syzygium polyanthum leave extract. Contrary, Eugenia aquea leave possessed higher contents of flavonoid than clove extract and Syzygium polyanthum extract. The clove leave extract showed higher DPPH free radical scavenging than Syzygium polyanthum and Eugenia aquea leave extract at 200 ppm level. Free radical scavenging activity of three extracts increased with increasing concentration of extract. The result showed also that clove phenolic extract possessed high potential as ingredient of sunscreen followed by Eugenia aquea and Syzygium polyanthum leave phenolic extract. Result of measuring the sun protection factor (SPF) were 34.38; 33.98 and 7.92, resvectively. These result suggested that three extracts in Myrtaceae family leave having potential as antioxidant and sun protection factor (SPF) value is high that including in low protection (6-8) and medium protection (>15).Keywords : antioxidant, Myrtaceae family, phenolic extract, sunscreen
EFEK LAMA PERENDAMAN EKSTRAK KALAMANSI (Citrus microcarpa) TERHADAP AKTIVITAS ANTIOKSIDAN TEPUNG PISANG GOROHO (Musa spp.) Kiay, Nancy; Suryanto, Edi; Mamahit, Lexie
CHEMISTRY PROGRESS Vol 4, No 1 (2011)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/cp.4.1.2011.26502

Abstract

ABSTRACTKiay et al., 2011. The effect calamansi (Citrus microcarpa) extract on antioxidant activity of goroho banana fluor(Musa spp.).Banana is local food commodity that used as sources of carbohydrate beside paddy. Fresh mature banana canbe produced as banana flour to prolong its lifetime and minimalize its nutrition degradation. Callamansi (Citrusmicrocarpa) contained antioxidant compound. Does callamansi extract improve antioxidant activity of gorohobanana flour. The aim of this research was to determine antioxidant activity of goroho banana flour whichimmersed with calamansi extract.This research using laboratory experiment methods to analyze total phenolic content, free radical scavengingactivity, total antioxidant and vitamins C of calamansi and goroho banana flour using spectrophotometer UV-vis.This research using complete random design (rancangan acak lengkap, RAL), thus obtained data wereanalyzed using statistic. Significant difference between groups were analyzed using ANOVA with p<0,05. Ifthere is significant difference between groups, data were analyzed using Duncan’s multiple range test (DMRT).The results showed that banana flour immersed with calamansi extract possess to total phenolic content 3,84mg/kg gallic acid, free radical scavenging activity 81,20%, total antioxidant 28,42 ppm and vitamins C 0,09mg/100 g sample (p>0,05) compared with vitamin C, citric acid and blank. Its caused by callamansi antioxidantdiffuse into banana flour. Banana flour immersed with vitamins C possess to total phenolic content 1,66 mg/kggallic acid, free radical scavenging activity 41,98%, total antioxidant 22,8 ppm and vitamins C 0,09 mg/100 gsample, Banana flour immersed with citric acid possess to total phenolic content 1,42 mg/kg gallic acid, freeradical scavenging activity 36,66%, total antioxidant 15,15 ppm and vitamins C 0,09 mg/100 g sample andblank possess to total phenolic content 1,49 mg/kg gallic acid, free radical scavenging activity 39,94%, totalantioxidant 16,63 ppm and vitamins C 0,09 mg/100 g sample. Antioxidant activity from calamansi extract andgoroho banana flour caused by it phenolics compound that act as antioxidant.As conclusions of this research, calamansi possess an antioxidant activity. Then goroho banana flour immersedwith calamansi extract possess high antioxidant activity. This research suggested that calamansi extract can beused to produce banana flour with antioxidant activity..Keywords : callamansi, goroho banana, flour, antioxidant
Co-Authors Abigael, Reliana Adisti A. Rumayar, Adisti A. Adithya Yudistira Adrian, Gerild Aliwu, Indriwati Alvin Febryanto Ginting, Alvin Febryanto Amelia Walangitan Aneke Korua, Aneke Audy Wuntu Chairil Anwar Chaleb Paul Maanari Christine Mamuaja Colling, Erningsi Dareda, Christina Tang Defny S. Wewengkang, Defny S. Dewa Gede Katja Dika, Oki Oktaviani Djasibani, Hemy R. Dokri Gumolung Edam, Mariati Edam, Mariati Elisa Putri Tarigan, Elisa Putri Ering, Aprily A. P. Etni Togolo Fahcrudin, M Fajar Lestari Fetty Indriaty FITRIA NINGRUM, FITRIA Frenly Wehantouw Gregoria S. S. Djarkasi Harry S. J. Koleangan Hasan Datu, Hasan Henry Aritonang Hindang S. Kaempe Indriawan, Rebecca Teisha Inneke F. M Rumengan Jeanette Manarisip Jemmy Abidjulu Jessy Paendong Johnly A Rorong Johnly A. Rorong Judith Mandei Juliana Christie Kurniawan Julius Pontoh Kabalmay, Julista Alfa Kaemba, Almawaty Kaemba, Almawaty Karepu, Meifry Gavrila Karisoh, Marsel Refanli Landeng, Patrisia Jaklin Landjang, Enrico Yoel Leo Arifsandi Budiarso, Leo Arifsandi Lexie Mamahit Lidya I. Momuat Lidya Irma Momuat Liemey Iviane Lumempouw Liemey Lumempouw Lisa Purnawati Saleh Luayli, Lina Makanaung, Eresia Mamuaja, Christin Max R. J. Runtuwene Meiske Lumingkewas Meiske S. Sangi Mercy Taroreh Momuat, Lidya Mongan, Jemsi Nancy Kiay, Nancy Nova . Olha Rantung, Olha Paijo, Auliya Rizky Harjono Palente, Ivon Pangemanan, David Albert Parinding, Yosep Rudol Pasanda, Immanuel M. Paulina V. Y. Yamlean Raganata, Tirza C. Riny Modaso Rizky H. Mawardi Rompas, Veckie Frankie Rondonuwu, Samuel D. J. Rorong, Johnly Alfrets Runtuwene, Max R. J Ryan Vanly Saryana Sakalaty, Evanda Selvian Talapessy Sembiring, Elia Sersermudy, Chesya Hana Shirley E. S. Kawengian Sri Febriani Hatam Sri Sudewi, Sri Susmantoyo, Adinda Putri Maharani Tapa, Fitriyanti La Taroreh, Mercy R. I. Taufik Hidayat Trina Tallei Waworuntu, Mutiara G. Yenny Tambunan