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All Journal Jurnal Ilmu Tanah dan Lingkungan (Journal of Soil Science and Environment) Agrikultura Microbiology Indonesia JTSL (Jurnal Tanah dan Sumberdaya Lahan) Journal of Degraded and Mining Lands Management AGRIVITA, Journal of Agricultural Science Jurnal Agroekoteknologi Jurnal Agro Kultivasi Scientific Contribution Oil and Gas Jurnal Agrotek Indonesia AGROLOGIA : Jurnal Ilmu Budidaya Tanaman Soil REns Jurnal Tanah dan Iklim Composite: Jurnal Ilmu Pertanian Media Kontak Tani Ternak

Pujawati Suryatmana

Departemen Ilmu Tanah Dan Sumberdaya Lahan Fakultas Pertanian Universitas Padjadjaran

Author-ID : 395349

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Earth & Planetary Sciences Energy Environmental Science Immunology & microbiology Medicine & Pharmacology Social Sciences Veterinary

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Karakteristik Azolla pinnata sebagai Pengganti Bahan Pembawa Pupuk Hayati Padat Bakteri Penambat N2 dan Bakteri Pelarut P Mieke Rochimi Setiawati; Pujawati Suryatmana; Amalia Chusnul
Soilrens Vol 15, No 1 (2017)
Publisher : Fakultas Pertanian Universitas Padjadjaran

The use of inorganic fertilizers for a long term can reduce soil fertility. One of the alternatives solving this problem is by using solid biofertilizers. Solid biofertilizer requires carrier materials that can support the viability of potential microbial within. Azolla pinnata is one of the carriers that has potential as a substitute for commonly used carrier materials. This study aims to determine the effect of Azolla pinnata and other organic materials with inoculant dosage on viability of bacterial population, N-total, P-total of solid biofertilizer. This research was conducted at Soil Biology Laboratory, Department of Soil Science, Faculty of Agriculture, Padjadjaran University in January-February 2015. The treatments were organic matter (Azolla pinnata, litter compost and peat) with various dosages of bacterial inoculant (20%, 25% 30%). This experiment used the Completely Random Design with factorial pattern. The main observations of the study were analyzed by ANOVA test and continued with Duncan test with 5% confidence level. The results showed that Azolla pinnata carrier has significantly effect to the increase of N-Total and P total of solid biofertilizer. The inoculant dosage of 30% affects the population increase of Azotobacter sp., and endophytic bacteria, but does not effect to the population of Phosphate Solubilizing Bacteria and Azospirillum sp. Azolla pinnata can be recommended as an alternative carrier that can replace peat and litter compost materials.The results of this study was illustrate Azolla pinnata can be recommended as an alternative carrier for solid biofertilizer that can replace peat and litter compost material with 30% inoculant dosage.Keywords: Azolla pinnata compost, litter, peat, solid biofertilizer

Pengaruh Jenis Bahan Organik dan Bakteri Pereduksi Sulfat Terhadap Konsentrasi Fe dan Mn dalam Remediasi Air Asam Tambang Apong Sandrawati; Pujawati Suryatmana; Ikrar Nusantara Putra; Nadia Nuraniya Kamaluddin
Soilrens Vol 17, No 1 (2019)
Publisher : Fakultas Pertanian Universitas Padjadjaran

Acid mine drainage (AMD) is one of the important issues in the environmental damage caused by coal mining activity. Remediation of AMD could be done by active and passive method. Passive method is cheaper and more efficient. Constructed wetland is one of passive method in AMD remediation. The objective of this research was to determine the effect of organic matter and sulphate reducing bacteria (SRB) combination to Fe and Mn concentration in water and substrate. The research used experimental design of Randomized Block Design (RBD) consisted of nine treatments and repeated three times. The result of this research showed that sulphate reducing bacteria and kind of organic matter combination significantly affected water pH, concentration of soluble Mn, and concentration of Fe and Mn in the substrate. Application of 100 % compost and SRB inoculation gave the best increase of water pH. Application 100 % sawdust with SRB inoculation provided the lowest concentration of soluble Fe in outlet water. Meanwhile, 100% sawdust application without inoculation of SRB increased of Mn soluble concentration in outlet water.

POTENSI Azotobacter sp. ST.04 DAN KOMPOS Azolla pinata UNTUK MENINGKATKAN EFISIENSI BIODEGRADASI HIDROKARBON DALAM PROSES BIOREMEDIASI LIMBAH MINYAK BUMI Pujawati Suryatmana; Mieke Rochimi Setyawati
Jurnal Agroekoteknologi Vol 6, No 1 (2014)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

The Petroleum hydrocarbon waste is one of Pollutant contributing which cause problem in environmental. Azotobacter is a rizobacteria type which is capable fixating dinitrogen. The result investigation showed that Azotobacter sp strain AV04 is capable producing many extra-cellulary biomolecule. That strain AV04 known is capable producing a biomolecule which acting as strong bioemulsifier. Azolla pinata is a hydro plant that can contributes of N, P dan K nutrition to the Soil. This plant has potentialy as a nutrition organic source. The research was focused on the role of the two agents, i.e. Azotobacter sp starin AV04 as the biosurfactan producing bacteria and Azolla pinata composted in the biodegradation hydrocarbon processes. Results showed that the Total Petroleum Hydrocarbon (TPH) efficiency in the 1st week and 5th week, which was in the treatment of 10% (w/w) of A. pinata and 2% (v/w) of Azotobacter sp starin AV04, was reaching in value 56.15 % and 98.03 % respectively. In the optimum treatment showed that the TPH removal efficiency consistently was significantly rising up as long as 5 week period of bioremediation. The residue compounds of Petroleum after one week period incubation on the optimum treatment were constituting hydrocarbon comprise of 26 species of hydrocarbon compound, while at the same time and in the control treatment founded the hydrocarbon compounds remain were still about 37 species of Hydrocarbon compound.

Efek Inokulasi Konsorsium Mikroba dan Aplikasi Nutrisi terhadap Populasi Azotobacter spp, Serapan N, Pertumbuhan Tanaman dan Hasil Tomat (Solanum lycopersicum L.) pada Sistem Hidroponik Pujawati Suryatmana; Mahdi Argawan Putra; Nadia Nuraniya Kamaluddin; Mieke Rochimi Setiawati; Betty Natalie Fitriatin; Reginawanti Hindersah
Soilrens Vol 19, No 1 (2021)
Publisher : Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/soilrens.v19i1.35089

Hydroponic system is an alternative agricultural cultivation technology and a potential system to overcome the decreasing availability of land. Tomato production with this system has its own market segment. Balanced nutrient management combined with inoculation of functional microbial consortium (N-fixing bacteria and phosphate solubilizers) is one of the efforts to increase tomato production in a hydroponic system. This experiment aimed to examine the effect of microbial consortium and hydroponic nutrition on tomato plant growth, Azotobacter spp. population, N uptake and tomato yield. The research design used was a factorial randomized block design (RBD) consisted of two factors. The first factor was the dose of the microbial consortium, consisting of three levels (0 mL/polybag, 5 mL/polybag, and 10 mL/polybag. The second factor was the nutritional dose consisting of three levels (0 mL, 250 mL, and 500 mL). The experimental results showed that there was no interaction between the microbial consortium treatment nutrients on hydroponic plants’ height, N uptake, Azotobacter spp. population and tomato yield. Increased nutrient dose significantly increased N uptake, with the highest yield at 500 ml/pot. But the increased nutrients dose did not lead to significantly different tomato yields. Inoculation of the microbial consortium did not significantly increase all tested parameters.

KARAKTERISTIK PERTUMBUHAN DAN WAKTU GENERASI ISOLAT Azotobacter sp. DAN BAKTERI ENDOFITIK ASAL EKOSISTEM LAHAN SAWAH Mieke Rochimi Setiawati; Pujawati Suryatmana; Diyan Herdiyantoro; Zahra Ilmiyati
Jurnal Agroekoteknologi Vol 6, No 1 (2014)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

Azotobacter sp. and N2-fixation endophytic bacteria are microbes that play a role in fixation the N2 from the free air and helps supply of N to plants and can make efficient use of N derived from inorganic fertilizer. N2-fixation microbes utilization in rice fields can reduce the cost of production of rice plants. By studying the characteristics of the two types of beneficial bacteria that is expected to be engineered to enhance the role of these bacteria contribute in supplying N to rice crops. The method used in this study is exploratory and observe the characteristics of growth and generation time of bacteria for 72 hours. The results of this study indicate that the bacterial isolates of Azotobacter sp-1 has the highest population in 48 hours with a total population of 1.58 x107 cfu ml-1 , whereas bacteria Azotobacter sp-2 has the highest population in 60 hours with a total population of 3.12 x106 cfu ml-1 . Both N2-fixation endophytic bacteria populations reached the highest peak in the 60th hour with a total population of N2-fixation endophytic bacteria-1 at 1.12 x 108 cfu ml-1 of N2-fixation endophytic bacteria-2 of 9.4 x 107 cfu ml-1 of both isolates endophytic bacteria showed a similar growth pattern. Azotobacter sp. isolates from rhizosfir of the rice plant have a difference generation time, while the two isolates of N2-fixation endophytic bacteria from tissue of rice plants having the same generation time. Isolates of Azotobacter sp.-1 had the fastest of generation time, capable of generating new cells every 158.66 minutes or every 2.64 hours.

EFFECTS OF PETROFILIC MICROORGANISMS AND BULKING AGENT ON HYDROCARBON’S BIODEGRADATION EFFICIENCY Zulkifliani, Zulkifliani; Suryatmana, Pujawati; Sylvia, Annisa Rosalina; Syafrizal, Syafrizal
Scientific Contributions Oil and Gas Vol 39, No 3 (2016)
Publisher : Scientific Contributions Oil and Gas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29017/SCOG.50.3.100

Bioremediation is a method which can be used to reduce the amount of pollutants or toxic substances that damage the environment through the use of microorganisms to remove the contaminants. The purpose of this research is to determine the effects of petrofilic microorganisms and bulking agent on the enhancement of hydrocarbon’s biodegradation efficiency, soil water content, growth rate of petrofilic bacteria and Azotobacter vinelandii. Nine treatments in this research were a petrofilic consortium (Pseudomonas spp., Actinomycetes sp., and petrofilic fungi) and Azotobacter vinelandii (biosurfactan), as well as bulking agent (rice husk charcoal and baglog waste of Oyster Mushrooms). The experiment design used was a randomized block design with three replications, so there were 27 experimental units (microscosmos). The result was that the application of petrofilic microorganisms and bulking agent is the best treatment to enhance the efficiency of hydrocarbon’s biodegradation, the growth rate of petrofilic bacteria, and soil water content. The petrofilic consortium treatment with the highest value of biodegradation efficiency reached 83,9%.

Peningkatan produktivitas tanaman kedelai kultivar Anjasmoro asal benih terdeteriorasi dengan kompos Trichoderma dan bokashi Sumadi Sumadi; Denny Sobardini Sobarna; Pujawati Suryatmana; Meddy Rachmadi; Erni Suminar
Kultivasi Vol 17, No 3 (2018)
Publisher : Universitas Padjadjaran

Sari. Trichoderma spp merupakan golongan fungi yang bersifat antipatogen, khususnya layu kecambah, sedangkan bokashi merupakan kompos yang diperkaya dengan beberapa mikroba yang bermanfaat bagi pertumbuhan tanaman.Tulisan ini merupakan rangkuman penelitian pot menggunakan kompos Trichoderma spp sebagai pelapis benih terdeteriorasi disertai bokashi kotoran hewan yang dilakukan pada tanah lahan kering dan tanah sawah pada tahun 2014 dan 2017. Percobaan pertama membandingkan efektivitas beberapa agen hayati sebagai pelapis benih pengaruhnya terhadap vigor benih dan hasil tanaman. Hasil percobaan menunjukkan penggunaan kompos Trichoderma hasilnya lebih baik dibandingkan agen hayati lainnya. Hasil percobaan selanjutnya menunjukkan bahwa pelapisan benih dengan 1 sampai 3 g kompos Trichoderma spp per 100 butir benih pengaruhnya tidak nyata dibandingkan dengan penggunaan pelapis benih berupa pestisida, baik terhadap vigor maupun hasil biji per tanaman. Pemberian bokashi sebaliknya secara nyata mampu meningkatkan hasil biji per tanaman . Pemberian bokashi 15 t ha-1menghasilkan biji seberat 19.83 g per tanaman atau setara dengan 2,379 t ha-1 atau 27,3 % lebih tinggi dari kontrol.Kata kunci : Benih terdeteriorasi, pelapisan benih, Trichoderma, Bokashi Abstract. Trichoderma spp is a group of antipatogenic fungi, especially seedling wilted, while bokashi is a compost enriched with some microbes that are beneficial to plant growth. This paper is a compilation of pot experiment using Trichoderma compost for coating deteriorated seed and accompanied by bokashi on dry land soil and paddy soil in 2014 and 2017 respectively. The first experiment compares the effectiveness of several biological agents as a seed coating of its effect on seed vigor and yield. The results of first experiment showed that the use of 2 g Trichoderma compost 100 seeds-1 was better than other biological agents. However between 1.2 and 3 g of Trichoderma compost per 100 seeds was not significant effect on seed vigor and yield. The results of experiment showed that the coating of seeds with 1 – 3 g of Trichoderma spp compost 100 compared with pesticide seed coatings. Increased of yield only affected bokashi application. Application of bokashi 15 t ha-1 abble to produce 19.83 g seed per plant or equivalent to 2.379 t ha-1 or 27.3% higher than the control.Key words: deteriorated seeds, seed coating, Trichoderma, bokashi

Keragaman Mikroflora, Mikrofauna, Kandungan C-organik, dan Total N Tanah Sawah Akibat Aplikasi Azolla dan Pupuk Hayati Mieke Rochimi Setiawati; Pujawati Suryatmana; Tualar Simarmata
Soilrens Vol 18, No 1 (2020)
Publisher : Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/soilrens.v18i1.29041

This study was conducted to determine the effect of Azolla pinnata and biofertilizers (Azotobacter and Phosphate Solubilizing Bacteria) on the total microflora, microfauna, organic C, N-total soil, and C/N ratio of soil in lowland rice. The study was carried out in the SPLPP experimental field (Agricultural Research and Development Research Studio) of the Faculty of Agriculture, Padjadjaran University, Jelekong Village, Ciparay, Bandung Regency, West Java, with an altitude of 628 meters above the sea level. The experimental design used was factorial randomized block design consisted of two factors and repeated four times. The first factor was the provision of Azolla with two levels: without Azolla and 3 tons ha-1 of Azolla pinnata. The second factor was the provision of biofertilizer with three levels, without biofertilizer, 5 L ha-1 biofertilizer, and 10 L ha-1 biofertilizer. The results showed that there was an interaction between A. pinnata with biofertilizer on the increase in the total bacterial population. Total fungus, microfauna (nematodes), and soils N-total content did not increase with the addition of Azolla or biofertilizers. Independently A. pinnata increased soil C-organic matter while biofertilizer 10 L ha-1 increased the total population of actinomycetes.

Pengaruh Amelioran Partikel Nano Batuan Fosfat dan Jamur Pelarut Fosfat terhadap Sifat Kimia Inceptisols Cilembu, Jawa Barat Fajri Syahid Nurhakim; Pujawati Suryatmana; Muhammad Amir Solihin; Rina Devnita; Mahfud Arifin
Jurnal Tanah dan Iklim (Indonesian Soil and Climate Journal) Vol 44, No 2 (2020)
Publisher : Balai Besar Penelitian dan Pengembangan Sumberdaya Lahan Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jti.v44n2.2020.155-162

Abstrak. Inceptisols Cilembu memiliki potensi besar untuk pertanian khususnya untuk budidaya ubi jalar Cilembu. Rendahnya kandungan P-tersedia yang merupakan unsur hara esensial berdampak pada produktivitas tanah. Aplikasi amelioran partikel nano batuan fosfat dan Jamur Pelarut Fosfat (JPF) memiliki kemampuan dalam meningkatkan P-tersedia Inceptisols Cilembu. Penelitian ini bertujuan untuk mengkaji pengaruh amelioran partikel nano batuan fosfat dan JPF terhadap beberapa sifat kimia Inceptisols di sekitar Cilembu. Metode penelitian menggunakan Rancangan Acak Lengkap Faktorial. Faktor pertama yaitu amelioran partikel nano batuan fosfat yang terdiri atas empat taraf, yaitu b0 = 0% (0 g), b1 = 2% (20 g kg-1 tanah), b2 = 4% (40 g kg-1 tanah), dan b3 = 6% (60 g kg-1 tanah). Faktor kedua yaitu JPF yang terdiri atas dua taraf, yaitu h0 = (0 g) dan h1 = JPF (10 g per kg-1 tanah). Parameter yang diamati: pH, P-tersedia, Kapasitas Tukar Kation (KTK), dan populasi JPF. Data hasil pengamatan dianalisis menggunakan analisis varians pada taraf 5%, dilanjutkan dengan uji beda jarak Duncan pada taraf 5%. Hasil analisis varians menunjukkan tidak terdapat interaksi antara amelioran partikel nano batuan fosfat dan JPF terhadap parameter yang diamati. Aplikasi amelioran partikel nano batuan fosfat berpengaruh nyata dalam meningkatkan pH dan P-tersedia. Penggunaan JPF berpengaruh nyata meningkatkan nilai KTK.Abstract. Inceptisols in Cilembu have a great potential for agriculture, especially for sweet potato cultivation. The low available P content, which is an essential nutrient, has an impact on soil productivity. The application of nano particle phosphate rock ameliorant and Phosphate Solubilizing Fungi (PSF) may increasing available P of Inceptisols in Cilembu. The research aimed to find out the effects of nano particle phosphate rock ameliorant and PSF on selected chemical properties of Inceptisols in Cilembu area. The research method used Factorial Completely Randomized Design. The first factor was ameliorant nano particles of phosphate rock application consisted of four levels, namely b0 = 0% (0 g), b1 = 2% (20 g kg-1 of soil), b2 = 4% (40 g kg-1 of soil), b3 = 6 % (60 g kg-1 of soil). The second factor was PSF treatment consisted of two levels, namely h0 = without PSF and h1 = PSF 10 g kg-1 of soil. Parameters observed were pH, available P, Cation Exchange Capacity (CEC), and PSF population. The data were analyzed with analysis of variance at the significance level of 5%, and continued with Duncan multiple range test at the significance level of 5%. The results showed that there was no interaction between ameliorant nano particles of phosphate rock application and PSF inoculation on the observed parameters. The application of ameliorant nano particles of phosphate rock significantly increased soil acidity (pH) and available P. The inoculation of PSF significantly increased the CEC.

Potensi Tanaman Sorgum (Sorghum bicolor L.), Azotobacter sp. dan Pseudomonas sp. Sebagai agen biologis dalam proses Fitoremediasi Hidrokarbon minyak bumi Pujawati Suryatmana; Alyani Shabrina; Nadia Nuraniya Kamaluddin; Betty Natalie Fitriatin; Reginawanti Hindersah; Mieke Rochimi Setiawati
Soilrens Vol 18, No 1 (2020)
Publisher : Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/soilrens.v18i1.29039

Phytoremediation using Sorghum (Sorghum bicolor L.) plant is an alternative green technology to overcome oil polluted soil. Improving of the phytoremediation performance are needed stimulating agent to enhance the rate of hydrocarbon biodegradation and increase the plant growth. One of the bioagent that can act as biostimulant is Azotobacter sp. and Pseudomonas sp., because thouse bioagent can provide elements N and P, and phytohormone for sorghum plants. The purpose of this study is to examine the role of Azotobacter sp. and Pseudomonas sp. as a biostimulant for Sorghum bicolor L. in the process of biodegradation of petroleum hydrocarbon in soil system. The factorial randomized block design was used as the experimental design. The treatment consisted of 4 application levels of Azotobacter sp. and 4 levels of application of Pseudomonas sp. The results showed that there was no interaction between Azotobacter sp. and Pseudomonas sp. aplication on the efficiency of hydrocarbon degradation of the population of Azotobacrer spp., Pseudomonas spp. and plant height increase. Azotobacter sp. and Pseudomonas sp. also did not show an increase in value on each test variable. While, the potential of sorghum plants without the application of Azotobacter sp. and Pseudomonas sp. (control treatment) showed the value of the efficiency of biodegradation in the range of values of 60.442% - 68.165% during 14 weeks period and not significantly different from other treatments.

Co-Authors . WISJNUPRAPTO Agung Karuniawan Alyani Shabrina Amalia Chusnul ANNE NURAINI Anne Nurbaity Anne Nurbaity Apong Sandrawati Aten Komarya Bagus Adityo Betty N. Fitriatin Betty Natalie Fitriatin Betty Natalie Fitriatin Camilla Salsabilla Christine Angel Dedeh H. Arief Denny Sobardini Sobarna Denny Sobardini Sobarna Diyah Sri Utami DIYAN HERDIYANTORO Diyan Herdiyantoro Diyan Herdiyantoro Diyan Herdiyantoro EDWAN KARDENA Eka Safitri Emma Trinurani Sofyan Enny Ratnaningsih Erni Erni Erni Suminar Evi Entang Fatimah Evi Entang Fatimah Fadhilla Oktavianingtyas Trisilvi Fajri Syahid Nurhakim Febby Nur Indriani Gordon Pius Marihot Harry Rum Haryadi Ibnu Rizki Perdana Ikrar Nusantara Putra Ikrar Nusantara Putra Indra Herliana Iva Fitriani Jihan Fitria Meilani Khumairah, Fiqriah Hanum Lia Nur Linda Mahdi Argawan Putra Mahfud Arifin Maya Damayani Maya Damayanti Meddy Rachmadi Mieke Rochimi S Mieke Rochimi Setiawati Mieke Rochimi Setiawati Mieke Rochimi Setiawati Mieke Rochimi Setiawati Mieke Rochimi Setiawati Mieke Rochimi Setiawati Mieke Rochimi Setiawati Mieke Rochimi Setiawati Mieke Rochimi Setiawati Mieke Rochimi Setyawati Muhammad Agus Mulyana Muhammad Amir Solihin Nadia N. Kamaluddin Nadia Nuraniya Kamaluddin Nanda Aditya Setyawan NENNY NURLAENY Ninda Meiditia Putri Nizar Ulfah Nurullita Fitri Qurnia Oviyanti Mulyani Probo Condrosari Rara Rahmatika Risanti Reginawanti Hindersah Reginawati Hindersah Rhazista Noviardi Rhazista Noviardi Richard A. Gunawan Ridha Hudaya Rija Sudirja Rina Devnita Shinta Nurrizqi Indrayani Silmi Rahadiana Putri Sumadi Sumadi Syafrizal Syafrizal SYARIFUL MUBAROK Sylvia, Annisa Rosalina Tarra Martiana Dewi Tualar Simarmata Tualar Simarmata Ummu ‘Azizah Halimah Uum Umiyati Vera Oktavia Subarja Yori Tridendra Yudhistari Sihombing Yuliati Machfud Yusup Hidayat Zahra Ilmiyati Zellya Handyman Zulkifliani, Zulkifliani

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