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All Journal Jurnal Ilmu Tanah dan Lingkungan (Journal of Soil Science and Environment) Agrikultura Microbiology Indonesia JTSL (Jurnal Tanah dan Sumberdaya Lahan) Journal of Degraded and Mining Lands Management AGRIVITA, Journal of Agricultural Science Jurnal Agroekoteknologi Jurnal Agro Kultivasi SAINS TANAH - Journal of Soil Science and Agroclimatology Scientific Contribution Oil and Gas Jurnal Agrotek Indonesia AGROLOGIA : Jurnal Ilmu Budidaya Tanaman Soil REns Jurnal Tanah dan Iklim Media Kontak Tani Ternak Composite: Jurnal Ilmu Pertanian

Pujawati Suryatmana

Department Of Soil Science And Land Resource Management, Faculty Of Agriculture, Universitas Padjadjaran, Indonesia

Author-ID : 395349

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Earth & Planetary Sciences Education Energy Environmental Science Immunology & microbiology Medicine & Pharmacology Social Sciences Veterinary

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Reducing Pb accumulation in roots of sweet potato under low lead-contaminated soil by Azotobacter inoculation Reginawanti Hindersah; Vera Oktavia Subarja; Pujawati Suryatmana; Rija Sudirja; Agung Karuniawan; Yusup Hidayat
Journal of Degraded and Mining Lands Management Vol 10, No 2 (2023)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15243/jdmlm.2023.102.4271

Agricultural soil is possibly threatened by lead (Pb) contamination due to the intensive use of fertilizers. The rhizobacteria were recommended for the bioremediation of soils contaminated by low concentrations of Pb. The experiment was conducted to observe the Azotobacter's ability to proliferate in Pb-contaminated broth and to decrease the Pb availability in soil, Pb uptake by sweet potato roots, and sweet potato growth. The resistance test was performed by growing five Azotobacter isolates in N-free broth with various Pb levels. A pot experiment was conducted in a factorial randomized block design to test three levels of Pb in soil and two Azotobacter isolates. The results showed that Azotobacter Azv4 and A. choroococcum were resistant to 100 mg L-1 Pb in N-free broth. In the pot experiment, Azotobacter Azv4 Inoculation caused less Pb in soil and roots of sweet potatoes grown in Pb-contaminated soil than A. choroococcum. Either Azotobacter or Pb soil did not influence vine length. However, Azv4 was more prominent in increasing branch number, root volume and length; higher Pb in soil reduced branch number but did not affect root parameters. Azotobacter Azv4 increased more shoot and root dry weight compared to A. choroococcum, but both isolates did not change the shoot-to-root ratio (S/R). The Pb contamination only reduced root dry weight and reduced the S/R. This research considered utilizing rhizobacteria Azotobacter for reducing Pb levels in soil and roots; and increasing sweet potato biomass.

Efektifitas Azotobacter sp. Dan Pseudomonas sp. Pada tanaman sorgum (Shorgum bicolor L.) dalam proses fitoremediasi limbah minyak bumi Pujawati Suryatmana; Mieke Rochimi Setiawati; Nadia Nuraniya Kamaluddin; Diyan Herdiyantoro
Jurnal Agro Vol 9, No 2 (2022)
Publisher : Jurusan Agroteknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/20146

Interaksi sinergis antara tanaman-bakteri dapat digunakan untuk meningkatkan kinerja fitoremediasi tanah yang terkontaminasi hidrokarbon (HC). Penelitian bertujuan untuk karakterisasi plant growth promoting rhizobacteria: Azotobacter sp. dan Pseudomonas sp. dalam kinerja fitoremediasi limbah minyak bumi menggunakan tanaman sorgum (Sorghum bicolor L.). Penelitian menggunakan rancangan acak kelompok faktorial (RAK-Faktorial) terdiri dari inokulasi Azotobacter sp. sebanyak 0%, 1%, 2%, dan 3% per konsentrasi total petroleum hydrocarbon (TPH), dan inokulasi Pseudomonas sp 0%, 1%, 2%, dan 3% per TPH. Hasil penelitian menunjukkan bahwa tidak ada interaksi antara inokulasi Azotobacter sp. dan Pseudomonas sp. terhadap seluruh variabel respon. Namun, efek mandiri terlihat pada efisiensi biodegradasi HC, yaitu inokulasi Azotobacter sp dosis 3% berbeda secara signifikan dengan kontrol pada fase ke 4 minggu setelah tanam (MST), tetapi tidak signifikan pada fase 14 MST. Inokulasi Pseudomonas sp. tidak memberikan efek yang signifikan antar perlakuan terhadap efisiensi biodegradasi HC pada dua periode pengamatan. Populasi Azotobacter sp. dan Pseudomonas sp. menurun pada periode 4 MST, selanjutnya meningkat pada periode 14 MST. Pertumbuhan tanaman sorgum mengalami retardasi selama 14 MST. Residu hidrokarbon terakumulasi lebih tinggi di bagian akar daripada di bagian tajuk tanaman. Azotobacter sp. dan Pseudomonas sp. berperan sebagai binding agent yang dapat menghambat serapan hidrokarbon oleh akar sorgum.ABSTRACTThe synergism interaction between plant-bacteria can be used to improve performance of phytoremediation hydrocarbon (HC) contaminated soil. The study aimed to characterize Azotobacter sp. and Pseudomonas sp. in petroleum phytoremediation performance using sorghum (Sorghum bicolor L). The research used a randomized block design with two factors: Azotobactersp. inoculation 0%, 1%, 2% and 3% per total petroleum hydrocarbon (TPH) concetration, and Pseudomonas sp inoculation: 0%, 1%, 2% and 3% per TPH concentration. The results showed there was no interaction between Azotobacter sp. and Pseudomonas sp. inoculation to each response variable. The independent effect of 3% Azotobacter sp. to biodegradation HC efficiency was significantly different from the control at phase 4 weeks after planting (WAP), but not significantly different at phase 14 WAP. Pseudomonas sp. effect did not show a significantly different between treatments on the hydrocarbon biodegradation efficiency in the two observation periods. Azotobacter sp. and Pseudomonas sp. population decreased at period 4 WAP, then increased at period 14 WAP. The Sorghum growth was retarded during 14 WAP. Hydrocarbon residues accumulated higher in the roots than in shoot. Azotobacter sp. and Pseudomonas sp. act as a binding agent that can inhibit the hydrocarbons uptake by sorghum roots.

PENGARUH PENAMBAHAN TOP SOIL INCEPTISOL DAN KOMPOS PADA TAILING AMALGAMASI TERHADAP PANJANG SULUR, DIAMETER SULUR DAN JUMLAH CABANG TANAMAN UBI JALAR (Ipomoea batatas L.) Indra Herliana; Pujawati Suryatmana; Reginawanti Hindersah; Rhazista Noviardi
Jurnal Tanah dan Sumberdaya Lahan Vol. 8 No. 1 (2021)
Publisher : Departemen Tanah, Fakultas Pertanian, Universitas Brawijaya

Tailings are waste generated from the processing of gold ore. The tailings in Kertajaya Village, Sukabumi are generally dumped into a pond or garden which is then used by the community to grow crops. The characteristics of the tailings, which contain low organic matter, microorganism activity, CEC and essential nutrients, make it necessary to improve their condition. This study aimed to determine the effect of adding top soil and compost to the tailings on the growth of sweet potatoes in the vegetative phase. This experiment used a factorial randomized block design with two factors, namely a top soil-tailing ratio of 3 levels (70: 30% w/w; 50: 50% w/w; and 30: 70% w/w) and a compost dose of 4. level (without compost (control); 10 t ha-1; 20 t ha-1; and 30 t ha-1) with 3 replications. The results showed that the combination of the addition of top soil ratios and the dose of compost to the tailings had no effect on the addition of tendrils length, tendril diameter and number of sweet potato branches during the vegetative phase. The addition of 70% top soil independently on the tailings had an effect on the highest increase in tendril length, tendril diameter and number of branches of sweet potato. Meanwhile, the application of compost dosage of 30 t ha-1 had the highest effect on the number of branches Â Â of sweet potato.

Pemanfaatan Limbah Rumah Tangga sebagai Sumber Nutrisi dalam Kegiatan Pertanian Urban Nadia Nuraniya Kamaluddin; Ummu ‘Azizah Halimah; Nanda Aditya Setyawan; Pujawati Suryatmana; Mieke Rochimi Setiawati
Media Kontak Tani Ternak Vol 5, No 1 (2023): Februari
Publisher : Fakultas Peternakan, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/mktt.v5i1.44658

Dalam mengatasi keterbatasan lahan hijau di perkotaan, masyarakat mulai melakukan penghijuan dipadatnya perkotaan atau biasa disebut dengan pertanian urban. Kegiatan pertanian urban dapat membantu meningkatkan udara segar dari sekian banyaknya polusi yang mengotori udara perkotaaan, mewujudkan pola hidup yang sehat, dan berkontribusi dalam menjaga ketahanan pangan. Hidroponik merupakan salah satu budidaya tanaman dengan memanfaatkan air sebagai media tanam.. Selain bahan anorganik yang dapat dimanfaatkan dalam kegiatan pertanian urban, sampah organik pun dapat diolah menjadi nutrisi bagi tanaman untuk menjadi mikroorganisme lokal. Mikroorganisme ini sangat bermanfaat bagi nutrisi tanaman. Pemanfaatan sampah organik dan anorganik ini menjadi solusi terbaik bagi masyarakat untuk mengolah limbah rumah tangga sekaligus bernilai tambah sebagai sumber nutrisi dalam pertanian urban.

Potensi Kompos, tepung Azolla dan Dedak sebagai bahan Pembawa Bakteri Pemfiksasi N (BPN) dalam meningkatkan pertumbuhan dan hasil gabah Padi Gogo pada Inceptisols Jatinangor Pujawati Suryatmana; Jihan Fitria Meilani; Nadia Nuraniya Kamaluddin; Tualar Simarmata
Soilrens Vol 20, No 2 (2022)
Publisher : Dept Ilmu Tanah & Sumberdaya Lahan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/soilrens.v20i2.45268

Rice is an important commodity in Indonesia because rice is the main source of carbohydrates for the Indonesian. Rice production in Indonesia relies on lowland rice, but the productivity of lowland rice is decreasing. One of the efforts to increase rice national production is utilizing upland rice plants. The problem with upland rice is its low productivity. Nitrogen-fixing biological fertilizers can be used to increase the productivity of upland rice plants. The effect of different doses of nitrogen fertilizer and nitrogen fixing bacteria with different carrier materials on the growth and yield of upland rice in Inceptisols Jatinangor was studied. Randomized block design with two factors: the recommended dose of urea fertilizer (100% and 50%) and the nitrogen-fixing bacteria factor with different carriers (compost, rice bran, and Azolla powder) were applied as an experimental design. The experimental results showed that the interaction of nitrogen fertilizer dose and nitrogen-fixing bacteria with different carriers had no significant effect on plant height, number of panicles, root shoot ratio, and weight of 1000 grains of upland rice plants. Application of nitrogen-fixing bacteria with compost, bran, and Azolla powder as carriers was able to increase the average weight of the filled grain higher than the control.

Pengaruh Pupuk Hayati Berbasis Azolla terhadap Nitrogen Tanah, Nitrogen Tanaman, Populasi Azotobacter, dan Hasil Tanaman Mentimun Pada Inceptisol Jatinangor Mieke Rochimi Setiawati; Evi Entang Fatimah; Diyan Herdiyantoro; Apong Sandrawati; Uum Umiyati; Pujawati Suryatmana
Soilrens Vol 20, No 2 (2022)
Publisher : Dept Ilmu Tanah & Sumberdaya Lahan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/soilrens.v20i2.45271

The low productivity of cucumber plants in Indonesia can be caused by several factors, one of which is fertilization. Fertilization on Inceptisol soil which has a low nitrogen content is needed to increase cucumber crop yields. The use of inorganic fertilizers is limited to avoid environmental pollution, so the use of biofertilizers can be an environmentally friendly option. The purpose of this study was to determine the effect of concentration and interval application of azolla extract based biofertilizer on the total soil N, plant N content, Azotobacter population and cucumber yield. The experiment was carried out from August to November 2021 at the Ciparanje Experimental Garden, Faculty of Agriculture, Padjadjaran University. This experiment used a Completely Randomized Block Design of a combination concentrations (0, 5, 10, 15, 20 mL.L-1) and time of application (1, 2, 3 times). The results showed that the application of azolla extract based biofertilizer with different concentrations and application times had a significant effect on the azotobacter population but had no significant effect on soil nitrogen levels, plant nitrogen concentrations, and cucumber yields. The addition of 20 mL/L 2 times application of azolla-based biofertilizer increased cucumber fruit weight by 35.97% compared to control but did not cause a significant increase in yield

Effect of Molybdenum on Population of Bacillus Consortium and Production of Indole 3-Acetic Acid Aliya Zahrah Adawiah; Sandra Amalia Riyadi; Reginawanti Hindersah; Pujawati Suryatmana
Agrologia Vol 12, No 1 (2023): Agrologia: Jurnal Ilmu Budidaya Tanaman
Publisher : Faculty of Agriculture, Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/ajibt.v12i1.1771

Molybdenum (Mo) is one of nutrients that contributes on process of fixing N2 and it’s contained in growth medium of N2-fixing bacteria. Bacillus is bacterium that widely used as biofertilizer because it can fix N2 in the atmosphere and produce phytohormones in form of Indole 3-Acetic Acid (IAA) which are benefical for plant growth. The purpose of this research was to determine the effect of Mo addition on Bacillus consortium in a growth medium of 2%molasses + 10grams peptone + 3grams beef extract against vegetative cell population, spore population and IAA hormone production after incubated for 3, 6 and 9 days. This research was conducted at Soil Biology Laboratory, Faculty of Agriculture, Padjadjaran University. The method to calculate total population of vegetative cells is the serial dilution plate method with 80ºC heating for 30 minutes for total spore population, while the production of IAA hormone was measured using UV-Vis spectrophotometer with Salkowski reagent at a wavelength of 530 nm. All data subjected to Student-t test with p ≤ 0.05. The results of the study by using the Student-t test showed that Mo increased the vegetative cell population on day 3 but did not affect the vegetative cell population on day 6 and 9. The spore population of the Bacillus consortium was not determined by Mo. The production of IAA hormone in cultures with Mo was not different from that in cultures without Mo.

Phosphate availability, P-uptake, phosphatase, and yield of maize (Zea mays L.) affected by kaolin based P-solubilizer and P fertilizer in Inceptisols Betty Natalie Fitriatin; Muhammad Nafariz Budiman; Pujawati Suryatmana; Nadia Nuraniya Kamaluddin; Dedi Ruswandi
Kultivasi Vol 22, No 1 (2023): Jurnal Kultivasi
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/kultivasi.v22i1.42847

Inceptisols have problem in phosphate availability. Soil P content is very low available to plants because it is bound by soil colloids. One of the efforts to increase the P nutrient in the soil in a sustainable way by using P-Solubilizers that can dissolve phosphate in the soil so that it is available for plants. The purpose of experiment was to determine the effect of the combination dose of kaolin based P-Solubilizer and P fertilizer for improving P availability, P uptake, phosphatase, and maize yield on Inceptisols. The kaolin-based P-Solubilizer was used a consortium of phosphate solubilizing microbes (PSM) consisting of Bacillus subtilis, Burkholderia cepacea, Pseudomonas mallei, and Trichoderma asperellum. This experiment was conducted in the experimental field of the Laboratory of Soil Chemistry and Plant Nutrition, Faculty of Agriculture, Universitas Padjadjaran, Jatinangor, from July to December 2021. The experiment used a randomized block design (RDB) method with nine treatments and three replications, with details of 0 P-Solubilizer + 0 P-fertilizer; 100% P-fertilizer; 100% P-solubilizer; and combination 50%, 75 %, 100%, and 150% P-solubilizer with 50%, 75%, and 100% P-fertilizer. P-solubilizer 100% recommended dose 50 kg ha-1 and P-fertilizer recommended dose 100 kg ha-1. The results showed that the dose of 100% P-Solubilizer (50 kg ha-1) + 75% P (75 kg ha-1) showed the best results in increased P-availability (346,93%), P-uptake (312,5%), Phosphate activity (33,5%), and maize yields (48,09%) compared to without application of P-solubilizer and P-fertilizer. This consortium isolate could be developed as a P-Solubilizer with the ability to increase the efficiency of P up to 25%.

Effect of NPK and Bacillus-coated NPK fertilizer on biomass, nutrient content in soil and nutrient uptake by lettuce Reginawanti Hindersah; Mieke Rochimi Setiawati; Pujawati Suryatmana; Betty Natalie Fitriatin; Fasa Aditya; Gita Bina Nugraha; Rara Rahmatika Risanti; Priyanka Asmiran
Kultivasi Vol 22, No 1 (2023): Jurnal Kultivasi
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/kultivasi.v22i1.43608

The Inoculation of beneficial soil microbes is an effective method for lowering doses of inorganic fertilizers. This study was aimed to observe and compare the effect of doses and formulas of Bacillus-coated NPK (BCN) and conventional NPK fertilizers on biomass, major macro-nutrient in soil and their uptake by shoots of lettuce (Lactuca sativa L.); as well as evaluate the potency of BCN for decreasing doses of NPK fertilizers. The greenhouse experiment was set up in a randomized block design with seven treatments and five replications. The treatments included one and a half doses of recommended NPK fertilizer and two BCN fertilizer formulas; control treatment was without any fertilizer. This experiment showed that NPK fertilizer had comparable effect with BCN on growth traits; but application of NPK and coated NPK had a potency to increase the fresh weight of lettuce up to 24-45% which was in line with the increase of shoot-to-roots ratio. The potassium (K) content in soil and their uptake in lettuce shoots depend on doses and type of NPK but Nitrogen (N) and Phosphorus (P) content in soil and in shoot were not determined by treatments. The results showed that the recommended NPK dose (200 kg/ha) for lettuce can be reduced up to 50%; moreover, 50% of BCN enabled to maintain the N, P and K uptake as well as the lettuce yield.

The effect of beneficial microorganism as biofertilizer application in hydroponic-grown tomato Mieke Rochimi Setiawati; Nandha Afrilandha; Reginawanti Hindersah; Pujawati Suryatmana; Betty Natalie Fitriatin; Nadia Nuraniya Kamaluddin
SAINS TANAH - Journal of Soil Science and Agroclimatology Vol 20, No 1 (2023): June
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/stjssa.v20i1.63877

The use of N2-fixing bacteria and growth hormone-producing rhizobacteria delivers nitrogen, enhances nutrients absorption by plants, and reduces the usage of inorganic fertilizers. Implementing biofertilizer in the hydroponic system as a means to reduce application of synthetic nutrient is recently in interest due to economic, food safety, and sustainability factors. This study determines the effects of biofertilizer dose on tomato yields in the hydroponics system. A randomized block design was utilized that consisted of seven treatments, namely 100% inorganic fertilizer and 0% biofertilizer (control), and various doses of inorganic nutrient combined with 25%, 50%, 75%, and 100% biofertilizer. The result illustrated that the application of biofertilizer augmented the population of endophytic bacteria, Azotobacter sp., Azospirillum sp., phosphate solubilizing bacteria, and nitrogen content. The distinct combination of biofertilizer did not alter the phosphorus and potassium content compared to control samples however resulted in superior tomato grade. The 50% inorganic fertilizer and 100% biofertilizer combination amplified the weight of the fruit by 36% compared to the control. This finding indicates that the application of biofertilizers in the hydroponic system for tomato plants is not only beneficial in minimizing the dosage of inorganic fertilizers but also enhancing the fruit quality.

Co-Authors . WISJNUPRAPTO Agung Karuniawan Aliya Zahrah Adawiah Alyani Shabrina Amalia Chusnul Anas Ramdhani ANNE NURAINI Anne Nurbaity Anne Nurbaity Anni Yuniarti Apong Sandrawati Aten Komarya Bagus Adityo Betty N. Fitriatin Betty Natalie Fitriatin Betty Natalie Fitriatin Betty Natalie Fitriatin Camilla Salsabilla Christine Angel Dedeh H. Arief Dedi Ruswandi Denny Sobardini Sobarna Denny Sobardini Sobarna Diyah Sri Utami DIYAN HERDIYANTORO Diyan Herdiyantoro Diyan Herdiyantoro Diyan Herdiyantoro EDWAN KARDENA Eka Safitri Emma Trinurani Sofyan Enny Ratnaningsih Erni Erni Erni Suminar Evi Entang Fatimah Evi Entang Fatimah Fadhilla Oktavianingtyas Trisilvi Fajri Syahid Nurhakim Fasa Aditya Febby Nur Indriani Fera Siti Meilani Fiky Yulianto Wicaksono Gita Bina Nugraha Gordon Pius Marihot Harry Rum Haryadi Ibnu Rizki Perdana Ikrar Nusantara Putra Ikrar Nusantara Putra Indra Herliana Iva Fitriani Jihan Fitria Meilani Khumairah, Fiqriah Hanum Leoni Silvia Lia Nur Linda Mahdi Argawan Putra Mahfud Arifin Maya Damayani Maya Damayanti Meddy Rachmadi Mieke Rochimi S Mieke Rochimi Setiawati Mieke Rochimi Setiawati Mieke Rochimi Setiawati Mieke Rochimi Setiawati Mieke Rochimi Setiawati Mieke Rochimi Setiawati Mieke Rochimi Setiawati Mieke Rochimi Setiawati Mieke Rochimi Setiawati Mieke Rochimi Setiawati Mieke Rochimi Setiawati Mieke Rochimi Setiawati Mieke Rochimi Setyawati Muhammad Agus Mulyana Muhammad Amir Solihin Muhammad Nafariz Budiman Nadia N. Kamaluddin Nadia Nuraniya Kamaluddin Nadia Nuraniya Kamaluddin Nadia Nuraniya Kamluddin Nadia Rachelita Nanda Aditya Setyawan Nandha Afrilandha NENNY NURLAENY Ninda Meiditia Putri Nizar Ulfah Nurullita Fitri Qurnia Oviyanti Mulyani Pirda Nurhopipah Priyanka Asmiran Probo Condrosari Rara Rahmatika Risanti Reginawanti Hindersah Reginawati Hindersah Rhazista Noviardi Rhazista Noviardi Richard A. Gunawan Ridha Hudaya Rija Sudirja Rina Devnita Sandra Amalia Riyadi Shinta Nurrizqi Indrayani Silmi Rahadiana Putri Sumadi Sumadi Syafrizal Syafrizal SYARIFUL MUBAROK Syifa Nabila Kurnia Sylvia, Annisa Rosalina Tarra Martiana Dewi Tualar Simarmata Tualar Simarmata Ummu ‘Azizah Halimah Uum Umiyati Vera Oktavia Subarja Yori Tridendra Yudhistari Sihombing Yuliati Machfud Yusup Hidayat Zahra Ilmiyati Zellya Handyman Zulkifliani, Zulkifliani

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