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KERUSAKAN SEL HATI DAN PENINGKATKAN KOLESTEROL SERUM MENCIT AKIBAT PEMBERIAN MINYAK GORENG BEKAS PAKAI Sutejo, Ika Rahmawati
IKESMA Vol 8, No 1 (2012)
Publisher : FKM - UNEJ

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Abstract

The purpose of this study were to prove that the administration of heated palm oil can cause hepatocytes damage and cholesterol serum level incerase in mouse. This research was an experimental research using 38 male Mus musculus, Swiss derived. They were divided into 3 groups; 9 were the first negative control by aquadest administration, 9 were the second negative control by fresh palm oil administration, and 20 were intervention by 27-times heated palm oil administration. The dose of administration was 1 ml/100 gram body weight, every day for 14 days. Parameter used in examining liver microscopically was the percentage of hepatocytes getting degeneration of each vision field by 100-times magnificence. The result of microscopic examination were normal hepatocytes for aquadest administration group, fatty degeneration for fresh palm oil administration group, fatty degeneration and central zone necrosis for 27-times heated palm oil group. The average value of cholesterol serum level in aquadest administration group was 101,44 ± 8,71 mg/dl, in fresh palm oil administration group was 104,66 ± 7,75 mg/dl, and in 27-times heated palm oil group was 130,15 ± 12,26 mg/dl. It could be concluded that administration of 27-times heated palm oil for 14 days can cause degeneration of hepatocytes (p<0,05) and cholesterol serum level increase (p<0,05). Keyword: heated palm oil, fatty degeneration, necrosis hepatocyte, cholesterol
In Vitro Study of the Combination of Doxorubicin, Curcuma xanthorrhiza, Brucea javanica, and Ficus septica as a Potential Novel Therapy for Metastatic Breast Cancer Sutejo, Ika Rahmawati; Putri, Herwandhani; Handayani, Sri; Jenie, Riris Istighfari; Meiyanto, Edy
Indonesian Journal of Pharmacy Vol 30 No 1, 2019
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1629.862 KB) | DOI: 10.14499/indonesianjpharm30iss1pp15

Abstract

Less optimized therapeutic effects constrain the use of doxorubicin as the main agent of chemotherapy for metastatic breast cancer, resistance and side effects. Therefore we need a combination of more than one chemopreventive agent which has different molecular targets to solve that problem. The aims of this study is to prove the inhibitory effect of ethanolic extract of rhizome of Curcuma xanthorrhiza (ECx), fruit of Brucea javanica (EBj), leave of Ficus septica (EFs) and doxorubicin (Dox) alone and its combination on migration and invasion of a highly metastatic 4T1 breast cancer cell line. Cytotoxic activity of single and combination treatment was evaluated by MTT assay, followed by an experiment of apoptosis induction by using flow cytometry. The inhibitory effect on migration was observed by the scratch wound-healing assay. Furthermore, the observation of the activity of matrix metalloproteinase-9 (MMP-9) was analyzed by gelatin zymography. The results showed that ECx, EBj, EFs, and Dox has cytotoxic activity on 4T1 cells with the value of IC50 respectively 49.7±1.53mg/mL, 59.9±1.79mg/mL, 15.2±2.12mg/mL and 1.2±0.23mM. Furthermore, combination of ECx-EBj-Dox and ECx-EBj-EFs revealed synergistic effect on 4T1 cells and decrease cell viability through the induction of apoptosis and necrosis. Based on wound healing assay, 24 hours incubation of this combination inhibited 4T1 cells migration compared to single treatment. Gelatin zymography analysis showed that this combination also inhibited the activity of MMP-9 greater than a single use. Curcuma xanthorrhiza, Brucea javanica, and Ficus septica may have potential to be developed as a combination with or without doxorubicin for metastatic breast cancer treatment.
The Selectivity of Ethanolic Extract of Buah Makassar (Brucea javanica) on Metastatic Breast Cancer Cells Sutejo, Ika Rahmawati; Putri, Herwandhani; Meiyanto, Edy
Journal of Agromedicine and Medical Sciences Vol 2, No 1 (2016)
Publisher : Medical Faculty of Jember University (Fakultas Kedokteran Universitas Jember)

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Abstract

Treatment of cancer such as surgery, radiotherapy and chemotherapy has many side effects. Chemopreventive agent is needed to reduce the side effect and increase the effectivity of therapy. The discovery of  cochemopreventive agent should consider on its selectivity to reduce side effects. The selective cochemopreventive agents work effectively in cancer cells and safe for normal cells. Buah Makassar (Brucea javanica) is a natural product that is empirically used for anti-inflammatory and antitumor. The purpose of this study is to determine the cytotoxic effect of ethanolic extract of buah Makassar against 4T1, MCF7, HeLa, and Vero cell lines. The cytotoxic test is performed by MTT assay. The parameter obtained from the cytotoxic test was IC50. Selectivity index is determined from IC50 ratio of cancer cells to normal cells. The results showed that ethanolic extract of buah Makassar has a cytotoxic activity on 4T1, MCF7, HeLa, and Vero cells with IC50 were 49,9±0,83 μg/mL; 107,6±8,14 μg/mL; 228,9±4,16 μg/mL and 395,5± 4,21 μg/mL respectively. It also has high selectivity on 4T1 metastatic breast cancer cell with selectivity index of 7,93. It can be concluded that the ethanolic extract of buah Makassar has potential to be delevoped as cochemopreventive agent especially on metastatic breast cancer. Keywords: Brucea javanica, MTT assay, selectivity index, 4T1, MCF7, HeLa, Vero
PENERAPAN TEKNOLOGI TEPAT GUNA PEMBUATAN SABUN BELERANG MENURUNKAN JUMLAH KASUS SKABIES SANTRI NURUL QARNAIN SUKOWONO JEMBER Ika Rahmawati Sutejo; Viddi Agustian Rosyidi
Jurnal Ilmu Kesehatan Masyarakat Vol 12 No 1 (2016)
Publisher : Fakultas Kesehatan Masyarakat, Universitas Jember

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Abstract

Latar Belakang: Skabies masih menjadi salah satu penyakit kulit utama santri pondokpesantren Nurul Qarnain desa Baletbaru kecamatan Sukowono. Penyakit ini menggangguproses belajar santri & menyebabkan produktivitas berkurang. Faktor yang mempengaruhiperkembangan penyakit adalah lingkungan kurang bersih dan personal higiene yang jelek.Kebersihan lingkungan dan fasilitas ponpes sudah sangat memadai sehingga faktor yangberperan utama menyebabkan penyakit adalah personal higiene yang jelek akibattidakpahamnya santri terhadap penyakit ini serta pengobatan awal yang kurang cepat dantepat.Tujuan Penelitian: Salah satu bahan penting untuk pencegahan dan pengobatan yaitu sabunbelerang harganya mahal, sehingga santri tidak membeli sabun jenis ini. Sehingga diperlukanpenerapan teknologi tepat guna berupa pelatihan pembuatan sabun belerang antiskabies.Metode Penelitian: Metode kegiatan berupa pendataan jumlah santri penderita skabies,penyuluhan tentang penyakit skabies dan pencegahannya, pelatihan pembuatan sabunbelerang antiskabies, dan pengkaderan santri untuk penanganan awal penyakit skabies.Seluruh rangkaian kegiatan dilaksanakan pada bulan Agustus-Oktober 2016 di pondokpesantren Nurul Qarnain Kecamatan Sukowono. Hasil Penelitian: Hasil penelitian awal menunjukkan 151 santri menderita skabies(prevalensi 13,48%) dan setelah kegiatan berakhir jumlah santri yang menderita skabies jauhberkurang dan tersisa sebanyak 15 santri. Kata kunci: skabies, teknologi tepat guna, sabun belerang, pondok pesantren
Prevalensi, Karakteristik dan Faktor-faktor yang Berhubungan dengan Penyakit Skabies di Pesantren Nurul Qarnain Kabupaten Jember (The Prevalence, Characteristic and Factors of Scabies in Pesantren Nurul Qarnain Jember) Ika Rahmawati Sutejo; Viddy Agustian Rosyidi; Alief Ilman Zaelany
Pustaka Kesehatan Vol 5 No 1 (2017)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Abstract

Scabies is a common skin disease caused by infection of Sarcoptes scabei, which is transmitted directly human-to-human contact especially in crowded community like pesantren. The characteristics of the students in pesantren are seemingly to be associated with Scabies. The purpose of this study was to determine the prevalence, characteristics of Scabies and its association with gender and education level of students in Pesantren Nurul Qarnain Jember. This cross sectional study was conducted to all students (1120 students) in 2016. Diagnosis was made based on anamnesis and dermatology examination. Data were analyzed using chi square test. The results showed that the prevalence of Scabies was 13.48% (male 24.89% and female 5.82%; education level tsanawiyah 15.14.1% and aliyah 10.85%). Most lesions were found in the interdigital space of the hands (40.40%). Chi square test showed significant difference between the prevalence of Scabies with gender (p=0.048) and education level (p=0.023) of the students. In conclusion, the prevalence of Scabies in Pesantren Nurul Qarnain Jember was 13.48% and there was association between the prevalence of Scabies with gender and education level of the students.  Keywords: Scabies, prevalence, students, gender, educational level
PHYTOESTEROGEN COMPONENT OF ETHANOLIC ROOT EXTRACT OF FLAXSEED (Boehmeria nivea) DECREASES CHOLESTEROL SERUM LEVEL ON OVARIECTOMIZED RATS Ika Rahmawati Sutejo; Hazmi Dwinanda Nurqistan; Imama Rasyada; Ni Nyoman Yuniasih; Annafira Yuniar; Rakhmat Ramadhani
NurseLine Journal Vol 1 No 1 (2016): May 2016
Publisher : Faculty of Nursing, Universitas Jember

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Abstract

Dyslipidemia is a major cause of morbidity, mortality and high cost of treatment. Dyslipidemia associated with coronary heart disease (CHD), which causes 4 million deaths every year. Estrogen, a hormone produced by women during menarche protect women from coronary heart disease. The decrease of estrogen production during menopause periode, higher the risk of dyslipidemia and coronary heart disease. Phytoestrogen therapy used to prevent dyslipidemia and coronary heart disease during menopause. hytoestrogens are estrogen-like compounds found in plants. Phytoestrogenic effects of Boehmeria nivea, which was used in this study, mainly contributed by isoflavone and lignan compounds. The purpose of this study was to prove the ability of ethanolic extract of root of Boehmeria nivea (EBN) to increase uterine weight and improve dyslipidemia on ovariectomized mice using a quasi-experimental with post test only design of research. Ovariectomized female rats were given an atherogenic diet of 3.5 cc egg yolk each day with an intravenous injection of 0.006 mg/200 gBB adrenaline. There are 6 groups consisting of a negative control, ovariectomized group, variectomized and atherogenic diet group, group with treatment of Boehmeria nivea (EBN) dose 125 mg/kg, 250 mg/kg, and 500 mg/kg. The results shows that there is a decrease of uterine weight on ovariectomized group compare to control group. Treatment of EBN increase uterine weight, decrease the levels of blood cholesterol and increase the levels of blood triglyceride. It can be concluded that fitoestrogen content of EBN improve lipid profile in ovariectomized rats.
Hubungan karakteristik pesenam terhadap keluhan nyeri lutut Ika Rahmawati Sutejo; I Nyoman Insan Parisudha; Septa Surya Wahyudi
MEDIKORA Vol 21, No 1 (2022): April
Publisher : Faculty of Sports Sciences, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/medikora.v21i1.45004

Abstract

Penelitian ini bertujuan untuk membuktikan hubungan karakteristik pesenam terhadap keluhan nyeri lutut. Penelitian ini merupakan penelitian analitik observasional dengan rancangan cross sectional. Populasi dalam penelitian ini adalah 60 orang, denagn rincian 30 untuk kelompok pesenam aerobik dan 30 untuk pesenam zumba. Kriteria inklusi yang dipakai adalah jenis kelamin wanita, frekuensi senam minimal satu kali dalam seminggu secara rutin selama minimal selama enam bulan, waktu senam dalam sekali latihan antara tiga puluh hingga enam puluh menit, dan telah mengisi informed consent sebelum pengisian kuesioner. Pengambilan data dilakukan dengan cara memberikan kuesioner mengunakan google form. Karakteristik pesenam yang dibandingkan pada penelitian ini antara lain frekuensi, usia, jenis senam, indeks massa tubuh (IMT), dan lama mengikuti senam dalam satu tahun. Skala nyeri lutut diukur menggunakan Number Rating Scale (NRS). Hasil analisis multivariat dengan uji logistik berganda menunjukkan hanya satu variabel yang berpengaruh signifikan, yaitu frekuensi senam dalam seminggu (p=0,044). Frekuensi senam berpengaruh terhadap nyeri lutut pesenam dengan Odds ratio (OR) sebesar 5,218. Karakteristik frekuensi senam berhubungan terhadap keluhan nyeri lutut (p=0,044) dengan nilai odds ratio 5,218. Usia tidak menunjukkan korelasi dengan keluhan nyeri lutut. Tidak ada hubungan IMT dengan nyeri lutut pesenam (p=0,178). Tidak ada hubungan jenis senam, baik zumba maupun aerobik dengan keluhan nyeri lutut. Lama mengikuti senam (dalam tahun) tidak berhubungan dengan keluhan nyeri lutut. Penelitian ini menyimpulkan frekuensi senam merupakan faktor risiko yang memengaruhi keluhan nyeri lutut pesenamThe correlation of gymnast characteristics with knee pain incidenceAbstracthis study aims to prove the relationship between the characteristics of the gymnast and complaints of knee pain. This research is an observational analytic study with a cross-sectional design. The population in this study was 60 people, with details of 30 for the aerobic gymnast group and 30 for the zumba gymnast. The inclusion criteria used were female gender, frequency of exercise at least once a week regularly for a minimum of six months, exercise time in one exercise between thirty to sixty minutes, and having filled out informed consent before filling out the questionnaire. Data collection was done by giving a questionnaire using a google form. The characteristics of the gymnasts compared in this study included frequency, age, type of exercise, body mass index (BMI), and length of time following the exercise in one year. The knee pain scale was measured using the Number Rating Scale (NRS). The results of multivariate analysis with multiple logistic tests showed that only one variable had a significant effect, namely the frequency of exercise in a week (p=0.044). The frequency of exercise has an effect on gymnast knee pain with an Odds ratio (OR) of 5.218. The characteristics of the frequency of exercise were associated with complaints of knee pain (p=0.044) with an odds ratio of 5.218. Age did not show a correlation with complaints of knee pain. There was no relationship between BMI and gymnast knee pain (p=0.178). There is no relationship between types of exercise, both Zumba and aerobics, with complaints of knee pain. Duration of exercise (in years) was not associated with complaints of knee pain. This study concludes that the frequency of exercise is a risk factor that affects the gymnast's knee pain complaints.
Literatur Review : Rapid Immunochromatography Sebagai Metode Skrining Kanker Serviks Berbasis Deteksi Onkoprotein HPV pada Urin Ika Rahmawati Sutejo; Kiky Martha Arieska
Jurnal Kesehatan Vol 8 No 3 (2020): Desember
Publisher : Politeknik Negeri Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25047/j-kes.v8i3.164

Abstract

Cervical cancer is still become main health problem especially in development country. This cancer is the second most common cancer in Indonesian women. It commonly caused by infection of Human Papillomavirus (HPV) type 16 and 18. Actually if cervical cancer was detected earlier, it shows better respond therapy than other cancers, but this opportunity is not supported by appropiate screening methods. Nowadays, pap smear and IVA are commonly used for detect cervical cancer but they only recommend for women who have had sexual intercourse. Both of them also require medical expertise and pap smear is highly cost. Based on these problem, this paper describes novel method for cervical cancer screening by using rapid immunochromatography kit which is more applicative, noninvasive, and affordable. This paper was a literature review (library research). Data obtained through the search engine using keywords: "Cervical Cancer", "HPV", "Urine", "Screening", “Lateral flow test”, and "Rapid immunochromatography". The references were taken from reliable journal in the range of 2009 to 2019. Data was also taken from textbooks and the health institution official sites which support the analysis. This method detect the presence of oncoprotein E6 & E7 of HPV-16 and HPV-18 in urinusing antigen-antibody binding principle. Sensitivity and specificity test done by comparing the results of the kit with the results of pap smear tests as a standard screening method and PCR HPV test on urinsamples. We conclude that with further protocol development and standardization to achieve clinical sensitivity, this kit is a solution for noninvasive detection of high risk cervical cancer so that treatment can be do immediately and the mortality rate due to cervical cancer can be reduced as much as possible.  
Comparison of the Effectiveness of 10% Sulfur Soap and 2-4 Ointment as Single Treatment Alief Ilman Zaelany; Ida Srisurani Wiji Astuti; Ika Rahmawati Sutejo
Journal of Agromedicine and Medical Sciences Vol 3 No 3 (2017)
Publisher : Faculty of Medicine, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/ams.v3i3.6145

Abstract

Scabies disease caused by Sarcoptes scabiei var. hominis mites has become a problem in a whole world especially in developing countries like Indonesia. Scabies has a very contagious nature especially in places with dense population levels like Islamic boarding school. Permethrin which is a drug of choice for scabies has a fairly expensive price so it is less effective when used in mass treatment. In Indonesia, sulfur preparations, especially 10% sulfur soap and 2-4 ointment are easy to obtain because the price is affordable and available at the community health clinic. The purpose of this study is to know the difference in effectiveness of 10% sulfur soap and 2-4 ointment as a single treatment and combination on scabies disease. In this clinical trial study, treatment was performed on 3 groups, 10% sulfur soap group (n=36), 2-4 ointment group (n=36) and combination group (n=36). The examination was done on days 8, 15 and 22. Data analysis using SPSS version 23 with chi-square test. The results showed that the highest recovery rate was in combination group (week-1 (22,22%), week-2 (63,89%) and week-3 (94,44%)) followed by 2-4 ointment group (week-1 (11,11%), week-2 (72,22%) and week-3 (88,89%)) and 10% sulfur soap group (week-1 (2,78%), week-2 (52,78%) and week-3 (75,0%). The results of the analysis comparing the 10% sulfur soap group with the combination group showed p value on all consecutive examinations p=0,013, p=0,339 and p=0,022. In the comparison 2-4 ointment group to the combination group obtained p value on all examinations respectively p=0,206, p=0,448 and p=0,394. The conclusion is combination therapy had the highest cure rate and statistically significant (p=<0,05) was obtained in comparison of 10% sulfur soap to combination at week-1 and week-3 examination. Keywords: scabies, comparison of the effectiveness, 10% sulfur soap, 2-4 ointment, combination, Islamic boarding school
Pemberdayaan Petani Desa Candijati dalam Pencegahan Covid-19 dan Peningkatan Status Ekonomi melalui Pembuatan Masker Jerami Aromaterapi Sutejo, Ika Rahmawati; Noveyani, Adistha Eka; Lukmawati, Avita
JURNAL KREATIVITAS PENGABDIAN KEPADA MASYARAKAT (PKM) Vol 5, No 1 (2022): Volume 5 No 1 Januari 2022
Publisher : Universitas Malahayati Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33024/jkpm.v1i1.5404

Abstract

ABSTRAK Mayoritas masyarakat Desa Candijati Arjasa Jember Jawa Timur bekerja di bidang pertanian  (64%). Desa Candijati merupakan penghasil padi terbanyak di Kecamatan Arjasa. Petani desa Candijati termasuk golongan masyarakat terdampak pandemi corona. Pembatasan mobilitas membuat petani kesulitan memasarkan produk pertaniannya. Harga produk pertanian menjadi anjlok. Pendapatan petani menjadi turun drastis. Hal ini mencetuskan kecemasan dan stress pada petani. Petani juga menghadapi ancaman terpapar covid selama pandemi, karena tidak mendapat informasi mengenai penyakit dan pencegahan yang benar. Berdasarkan permasalahan tersebut, tim pengusul pengabdian menawarkan solusi kepada mitra petani kelompok tani Desa Candijati berupa penyuluhan mengenai penyakit covid dan pencegahannya serta pelatihan membuat masker beraromaterapi. Tujuan pengabdian ini adalah mencegah paparan Covid dan mengurangi stress pada petani serta meningkatkan status ekonomi petani. Metode kegiatan terdiri dari identifikasi keluhan/penyakit petani, pemberian penyuluhan mengenai penyakit dan pencegahannya, dan pelatihan pembuatan tisue  aromaterapi. Tisue aromaterapi yang terbuat dari jerami dan ditambahkan aroma jeruk disisipkan ke dalam masker kain 2 lapis. Selain mencegah paparan virus covid, aroma jeruk pada tisue saat dipakai dalam masker dapat meredakan kecemasan sehingga membantu mengurangi stress yang dialami petani. Sasaran kegiatan ini adalah ibu-ibu petani desa Candijati kecamatan Arjasa. Hasil akhir kegiatan ini berupa produk masker aromaterapi, peningkatan ketrampilan,  pemahaman dan kesehatan masyarakat. Kata Kunci: masker, aromaterapi, tisue, jerami, petani  ABSTRACT The majority of the people of Candijati Arjasa Jember East Java work in agriculture (64%). Candijati village is the largest rice producer in the Arjasa district. Candijati farmers are part of the community affected by the corona pandemic. Restrictions on mobility make it difficult for farmers to market their agricultural products. Prices of agricultural products have fallen. Farmers' incomes have fallen drastically. This triggers anxiety and stress in farmers. Farmers also face the threat of being exposed to covid during the pandemic, because they do not get information about the disease and proper prevention. Based on these problems, the team offered solutions to farmers of Candijati Village in the form of counseling about the covid disease and its prevention as well as training to make aromatherapy masks. The purpose of this program is to prevent exposure to Covid, reduce stress, and improve the economic status of farmers. The method of activity consists of identifying farmer complaints/diseases, providing counseling about diseases and their prevention, training in making aromatherapy tisue. This aromatherapy tissue made from straw and added with the aroma of citrus is inserted into a 2-ply cloth mask. In addition to preventing exposure to the covid virus, the smell of citrus on tissues when used in masks can relieve anxiety so that it helps reduce stress. The target of this activity is the farmers of Candijati village, Arjasa sub-district. The end result of this activity is aromatherapy mask products, skill improvement, understanding, and public health.  Keywords: masks, aromatherapy, tissue, straw, farmers