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I Nengah Suwastika
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EKSTRAK DAUN TUMBUHAN MACARANGA TANARIUS (L.) M.A MENGHAMBAT LAJU PERTUMBUHAN BAKTERI STAPHYLOCOCCUS EPIDERMIDIS Rosidah, Maya Sofiyanti; Lambui, Orryani; Suwastika, I Nengah
Natural Science: Journal of Science and Technology Vol 7, No 1 (2018): Volume 7 Number 1 (March 2018)
Publisher : Univ. Tadulako

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Penelitian uji daya hambat ekstrak daun Macaranga tanarius (L.) M.A terhadap pertumbuhan bakteri Staphylococcus epidermidis. Tujuan penelitian ini yaitu untuk mengetahui daya tahan bakteri S. epidermidis terhadap ekstrak daun tumbuhan M. tanarius (L.) M.A, serta untuk mengetahui  konsentrasi ekstrak daun M. tanarius (L.) M.A yang efektif dalam menghambat pertumbuhan bakteri. Metode maserasi digunakan dalam preparasi ekstrak daun. Medium pertumbuhan bakteri yang digunakan adalah Luria Bertony (LB) dan inkubasi dilakukan pada suhu ruang selama 10 jam dengan agitasi 120 rpm. Parameter yang diukur adalah laju  pertumbuhan sel bakteri secara spektrofotometrik, penelitian ini di disusun dalam Rancangan Acak Lengkap (RAL) dengan 6 perlakuan dan 3 kali ulangan. Perlakuan yang dilakukan yaitu pemberian ekstrak pada konsentrasi 0%, 10%, 20%, 40%, 60%, serta kontrol positif menggunakan amoxicillin 2%. Hasil penelitian menunjukkan bahwa konsentrasi ekstrak daun 60% menyebabkan penghambatan  laju pertumbuhan pada fase logaritmik 4 jam setelah perlakuan.
DAFTAR ISI NATURAL SCIENCE: JOURNAL OF SCIENCE AND TECHNOLOGY Suwastika, I Nengah
Natural Science: Journal of Science and Technology Vol 8, No 2 (2019): Volume 8 Number 2 (August 2019)
Publisher : Univ. Tadulako

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Daftar Isi Natural Science: Journal of Science and Technology
UJI EFEKTIVITAS EKSTRAK DAUN TAHI AYAM (LANTANA CAMARA L.) SEBAGAI HERBISIDA ALAMI TERHADAP PERKECAMBAHAN BIJI AKASIA BERDURI (ACACIA NILOTICA (L.) WILLD. EX DELILE) Mirnawati, Mirnawati; Pitopang, Ramadhanil; Suwastika, I Nengah
Natural Science: Journal of Science and Technology Vol 6, No 2 (2017): Volume 6 Number 2 (August 2017)
Publisher : Univ. Tadulako

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Penelitian tentang ?Uji Efektivitas Ekstrak Daun Tahi Ayam (Lantana camara L.) sebagai Herbisida Alami Terhadap Perkecambahan Biji Akasia Berduri (Acacia nilotica (L.) Willd. ex Delile)? telah dilaksanakan pada bulan April sampai Desember 2016 di Laboratorium Jurusan Biologi Unit Bioteknologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Tadulako. Tujuan Penelitian ini untuk mengetahui konsentrasi pemberian ekstrak daun L. camara yang paling efektif sebagai herbisida alami dan bagaimana pengaruhnya terhadap perkecambahan biji A. nilotica. Penelitian didesain menggunakan Rancangan Acak Lengkap (RAL) yang terdiri atas lima perlakuan dan tiga ulangan dengan konsentrasi ekstrak P0= 0%, P1= 5%, P2= 10%, P3= 20%, P4= 30%. Variabel pengamatan meliputi daya kecambah (persentase perkecambahan), hari munculnya kecambah, panjang hipokotil, dan berat basah. Hasil penelitian menunjukkan bahwa pemberian ekstrak daun L. camara berpengaruh nyata menghambat perkecambahan biji A. nilotica, semakin tinggi konsentrasi ekstrak maka akan semakin berpotensi menghambat perkecambahan yang ditunjukkan dengan menurunnya persentase perkecambahan dan melambatnya munculnya kecambah serta rendahnya nilai pengukuran panjang hipokotil dan berat basah. Terdapat efektivitas herbisida alami  ekstrak daun L. camara pada konsentrasi 30% yang dapat digunakan sebagai salah satu alternatif untuk menghambat perkecambahan dan anakan A. nilotica.
INDUKSI KALUS DAN METABOLIT SEKUNDER TANAMAN BROTOWALI (TINOSPORA CRISPA L.) PADA MEDIUM MS DENGAN PENAMBAHAN ZPT 2,4-D DAN AIR KELAPA SECARA IN VITRO Sukmawati, Dewi; Herlina, Herlina; Muslimin, Muslimin; Suwastika, I Nengah
Natural Science: Journal of Science and Technology Vol 7, No 2 (2018): Volume 7 Number 2 (August 2018)
Publisher : Univ. Tadulako

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Penelitian ini bertujuan untuk mengetahui pengaruh penambahan air kelapa pada medium MS dengan kombinasi 2,4-D dalam mendorong pertumbuhan kalus dan produksi metabolit sekunder pada tanaman Brotowali (Tinospora crispa L.) secara in vitro. Penelitian ini disusun berdasarkan pada Rancangan Acak Lengkap (RAL) dengan 5 perlakuan dan 5 ulangan, media perlakuan yang digunakan adalah air kelapa 10% dan  2,4 Dikhlorofenoksiasetat (2,4-D)  dengan konsentrasi berbeda yaitu 2,0 mg/L, 2,5 mg/L, 3,0 mg/L, 3,5 mg/L, dan 4,0 mg/L. Parameter pengamatan terdiri dari waktu munculnya kalus, persentase eksplan yang membentuk kalus, berat kalus, warna kalus, tekstur kalus dan  produksi metabolit sekunder. Perlakuan M3 (MS0+ Air kelapa 10% + 3,0 mg/L) pada parameter berat kalus menunjukkan pengaruh yang nyata dari perlakuan. Warna kalus yang dihasilkan adalah kecoklatan (Browning), memiliki tekstur intermediet dan kalus mampu menghasilkan Flavonoid.
STUDI KEANEKARAGAMAN SPESIES NYAMUK ANOPHELES SP. DI KABUPATEN DONGGALA, PROVINSI SULAWESI TENGAH Fahmi, Mohammad; Fahri, Fahri; Nurwidayati, Anis; Suwastika, I Nengah
Natural Science: Journal of Science and Technology Vol 3, No 2 (2014): Volume 3 Number 2 (August 2014)
Publisher : Univ. Tadulako

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The aim of this study is to determine the diversity of morphological and genetic variation of the Anopheles sp. This study was conducted in the periode of November 2013 - February 2014 in two (2) malaria endemic areas; Labuan Village, in North Donggala and Lalombi Village, in South Banawa of South Donggala Regency. Sample collection was done around cattle cage throughout the night between 18:00 to 6:00. The capture period was 15 minutes oh each, and it was done in every hour. The collection was done by using an aspirator and the sampel was stored in paper cup prior covered by gauze pads. Morphological identification of samples was performed at Entomology Laboratory, Vector Borne Disease Research and Development Unit Donggala. Identification was done based on O'connor and Soepanto (1981). Analysis of genetic was done by RAPD-PCR method. Morphological identification found that there were three (3) species of mosquitoes from the two (2) sites, namely An. tesselatus, An. subpictus, and An. vagus. The highest spesies diversity index obtained in the Lalombi village with H' = 1,07 and the lowest value in the Labuan village with a value of  H' = 0,33. RAPD analysis showed that there were similarity on DNA amplification band patterns on An. tesselatus from the village of Labuan  and it from  Lalombi. But interestingly, there were different on DNA amplification pattern of An. vagus from these two sites.  This  results indicating that the there was genetic variation on An. vagus from these two different villages, even though its have similarity in morphological characters.
PERBANYAKAN TANAMAN MELON (CUCUMIS MELO L.) SECARA IN VITRO PADA MEDIUM MS DENGAN PENAMBAHAN INDOLE ACETIC ACID (IAA) DAN BENZIL AMINO PURIN (BAP) Lidyawati, Ni Nyoman; Waeniati, Waeniati; Muslimin, Muslimin; Suwastika, I Nengah
Natural Science: Journal of Science and Technology Vol 1, No 1 (2012)
Publisher : Univ. Tadulako

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The research on organogenesis of melon (Cucumis melo L.) was done at Tissue Culture Laboratory Forestry Faculty UNTAD Palu, during January until May 2012. The aim of this research was to determine the best concentration of IAA and BAP hormones in inducing organs of this plant.  Sterile seedling of melon was used as explants in this work. The experiment was arranged in  Completely Randomized Design (CRD)  with  4 treatments and 4 replications. The treatments were MS0 + 0,1 ppm IAA + 0,5 ppm BAP (M1), MS0 + 0,1 ppm IAA + 0,7 ppm BAP (M2), MS0 + 0,1 ppm IAA + 0,9 ppm BAP (M3) and MS0 + 0,1 ppm IAA + 1 ppm BAP (M4). Parameters observed on this study were the day appear of shoot and leaves, the number of shoots and leaves and also the present of callus and root. The result showed that the best medium for organs induction was MS0 + 0,1 ppm IAA + 1 ppm BAP, which shoot and leaf emerged in the day of 3,75 and 5,75 after induction, respectively. This media was also induced the number of shoots and leaves, i.e 4,75 and 8,75, respectively. Nevertheles this media was not suitable enough in inducing root formation.
UJI DAYA HAMBAT DAN SKRINING FITOKIMIA EKSTRAK DAUN MACARANGA TANARIUS (L.) MULL. ARG SEBAGAI ANTIBAKTERI SALMONELLA TYPHI Musdalifah, Musdalifah; Khumaidi, Akhmad; Suwastika, I Nengah
Natural Science: Journal of Science and Technology Vol 6, No 3 (2017): Volume 6 Number 3 (December 2017)
Publisher : Univ. Tadulako

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Abstract

Penelitian uji daya hambat dan skrining fitokimia ekstrak daun Macaranga tanarius (L.) M.A sebagai antibakteri Salmonella typhi telah dilaksanakan pada bulan Oktober sampai November 2016. Penelitian ini bertujuan untuk mendeskripsikan morfologi tumbuhan, mengetahui aktifitas antibakteri, serta kandungan kimia ekstrak daun M.  tanarius (L.) M.A. Penelitian in menggunakan metode deskriptif, analisis skrining fitokimia,dan eksperimental dalam uji daya hambat ekstrak yang disusun dalam Rancangan Acak Lengkap (RAL) dengan 6 perlakuan dan 3 ulangan. Perlakuan meliputi pemberian konsentrasi ekstrak daun 10%, 20%, 40% dan 60%, serta kontrol positif (kloramfenikol 1%) dan kontrol negatif (Na-CMC 1%). Hasil penelitian menunjukan bahwa ekstrak  tumbuhan M. tanarius (L.) M.A pada konsentrasi 60% menghasilkan zona hambat yang paling besar yaitu 20 mm, lebih tinggi dibandingkan konsentrasi lainnya, namun masih lebih kecil dibandingkan kontrol positif. Hasil skrining fitokimia menunjukan adanya senyawa alkaloid, tanin dan saponin pada tumbuhan Macaranga tanarius (L.) M.A
BACTERIAL MER GENES ARE POTENTIAL GENETIC MATERIAL FOR BIOREMEDIATION ON MERCURY POLUTION Suwastika, I Nengah
Natural Science: Journal of Science and Technology Vol 2, No 1 (2013): Volume 2 Number 1 (March 2013)
Publisher : Univ. Tadulako

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Remediation over mercury-contaminated areas become major concern and needs to be solved. Recently, various parts of ecosystem were contaminated because of increase of mercury emission into environment. Methyl-mercury is the most toxic form, which commonly found in contaminated area. This pollutant was rapidly increased due to uncontrolled emission of mercury from power plan, gold mining process and from other sources. The one possible and reasonable method in mercury bioremediation is by using genetically modified plant expressing bacterial-mercury resistance gene. Group of Mer gene was characterized encode mercury transporter and mercury detoxification protein in bacteria. On this study, Blast search on database based on known Mer protein sequence, it shows that these proteins were conserved among bacteria. These groups of genes are potentially to be cloned and transformed into plant for phytoremediation purposes. In order to enhance mercury uptake and reduce its toxicity, multiple genes transformation are considered to be effective approach for transgenic plant construction. This transgenic plant should survive in medium with high concentration of mercury and it should have activities in mercury uptake, detoxification, and Volatilization.
STUDI BEBERAPA ASPEK BOTANI AMORPHOPHALLUS PAEONIIFOLIUS DENNST. NICOLSON (ARACEAE) DI LEMBAH PALU Jintan, Jintan; Yuzammi, Yuzammi; Suwastika, I Nengah; Pitopang, Ramadhanil
Natural Science: Journal of Science and Technology Vol 4, No 1 (2015): Volume 4 Number 1 (March 2015)
Publisher : Univ. Tadulako

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Penelitian tentang tumbuhan Amorphophallus paeoniifolius Dennst. Nicolson di Lembah Palu dilakukan dengan metode survey dan metode deskriptif pada bulan Desember 2011 sampai April 2012.  Penelitian ini bertujuan untuk mempelajari beberapa aspek botani dari tumbuhan Amorphophallus paeoniifolius meliputi anatomi, ekologi (pengukuran faktor lingkungan abiotik, biotik, sosial ekonomi masyarakat) serta  morfologi. Pengamatan faktor lingkungan biotik  dan pengambilan sampel dilakukan di dua lokasi dengan menggunakan 5 plot pada tiap lokasi untuk tingkat pohon, sapling dan anakan, serta pengamatan jenis fauna. Penelitian deskriptif meliputi identifikasi jenis Amorphophallus, morfologi dan anatomi, dilakukan di Herbarium Celebense dan Laboratorium Bioteknologi. Hasil pengamatan anatomi menunjukkan adanya dua tipe stomata berbeda pada daun dan bunga, serta pigmen warna bunga yang terdiri dari antosianin, xantofil dan klorofil. Pohon yang dominan di lokasi penelitian adalah  Cocos nucifera  dan Lannea grandis.  Jenis fauna teridentifikasi adalah burung Pignonotus aurigaster dan serangga Scarabaeidae (Onthophagus sp) yang berperan penting dalam Amorphophallus yaitu sebagai penyebar biji dan polinator. Amorphophallus termasuk famili Araceae memiliki daun lengkap, majemuk dan batang semu warna hijau dengan totol putih. Merupakan modifikasi batang (umbi). Bunga majemuk tipe tongkol (spadix), dengan bunga jantan dan betina yang bersifat protogeni, serta mengeluarkan bau khas.
ORGANOGENESIS TANAMAN BAWANG MERAH (ALLIUM ASCALONICUM L.) LOKAL PALU SECARA IN VITRO PADA MEDIUM MS DENGAN PENAMBAHAN IAA DAN BAP Rufaida, Anna; Waeniaty, Waeniaty; Muslimin, Muslimin; Suwastika, I Nengah
Natural Science: Journal of Science and Technology Vol 2, No 2 (2013): Volume 2 Number 2 (August 2013)
Publisher : Univ. Tadulako

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Research on plant organogenesis Shallots (Allium ascalonicum L.) local Palu on MS medium with the addition of IAA and BAP, have been carried out in March and July 2013 in the Tissue Culture Laboratory, Faculty of Forestry Tadulako University. This study aims to get a combination of the concentration of IAA and BAP in MS medium onion plant organogenesis spur. Explants were used in the form of lateral shoots from the onion bulbs. This experiment is based on a complete randomized design (CRD) with 4 treatments, each treatment was repeated 5 times and every single unit test using a 2 explants. Growth media as treatments tested were: A1 (MS0 + 0.01 ppm IAA + 1 ppm BAP), A2 (MS0 + 0.05 ppm IAA + 1 ppm BAP), A3 (MS0 + 0.1 ppm IAA + 1 ppm BAP), A4 (MS0 + 0.5 ppm IAA + 1 ppm BAP). Judging from the appearance of the root, plantlet height, number of leaves, number of shoots, number of roots, chlorophyll content and the number of stomata per explant cultures tested The results showed that all treatments tested were able to induce organ onion crop Local Palu.. Based on these parameters, A1 is the best media in promoting organogenesis Local onion Palu. The media gives the best results appear to speed root, shoot number, leaf number and chlorophyll content. Other than that, the media A4 is also the best medium for the initiation stage plantlets before acclimatization. The media gives the best results for the number of stomata, while emerging roots and number of roots.
Co-Authors Agung, Muhammad Budi Akhmad Khumaidi Ali Djamhuri Anis Nurwidayati Anna Rufaida Aras, Mohammad Rezha Ari Sulistiami Asri Pirade Paserang Bill Yorsan Yonathan Cindy Claudia, Cindy Dana Sulistia ENY YUNIATI Fadhalna Fadhalna Fahri Fahri Fahri Fahri Felma Tri Utami Frastika, Dian Gazali, Faris Muhammad Haliana Haliana Haliani Haliani Handayani, Mutia Harliana Harliana Haryati Baktiar Herlina Herlina Ihwan Ihwan Imelda, Silvia Jintan Jintan Laksono Trisnantoro Lina Mahardiana Marina Marina Mazidaturohmah, Mazidaturohmah Melisa S.M. Sorentina Meryany Ananda Mirnawati Mirnawati Mohammad Fahmi Musdalifah Musdalifah Muslimin Muslimin Ni Nyoman Lidyawati Niluh Made Dwi PYD Ningsi, Putri Suria Novika Nurul Aisyah Nurvita Nurvita Orryani Lambui Paserang, Asri P. Pranata, Adhi Rahmansyah Rahmansyah Rahmat Rahmat Ramadanil Pitopang Ramadhanil Pitopang Ramadhanil Pitopang Ramadhanil pitopang Rifka Rifka Rifka Rifka Riska Tajuddin Rosidah, Maya Sofiyanti Sahril M Taher Sari, Riska Sri Niken Ariati Sri Utami Talantan, Vrillia Marannu Umrah Umrah Urfiana Urfiana Waeniati Waeniati Waeniaty Waeniaty Weaniati Weaniati Wilma Wilma Yundari, Yundari Yunus, Fahrunnisa Yuzammi Yuzammi