Article Per Year (5 Year)
p-Index From 2019 - 2024
0
P-Index
This Author published in this journals
All Journal Jurnal Penelitian Farmasi Indonesia
desriany astina
Unknown Affiliation
Medicine & Pharmacology

Published : 1 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 1 Documents
Search

 1 
PENENTUAN AKTIVITAS ENZIM LACCASE Rhus vernicifera MENGGUNAKAN GUAIACOL SEBAGAI SUBSTRAT desriany astina
Jurnal Penelitian Farmasi Indonesia Vol. 5 No. 2 (2016): jpfi
Publisher : Lembaga Penelitian Sekolah Tinggi Ilmu Farmasi Jl. Kamboja Simpang Baru-Panam, Pekanbaru, Riau 28293 Telp. (0761) 588006, Fax. (0761) 588007 e-mail: editor-jpfi@stifar-riau.ac.id

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (240.571 KB)

Abstract

The enzyme laccase (EC.1.10.3.2: oxygen oxidoreductase) is an extracellular enzyme that has a benefit in various industrial fields. This enzyme catalyzes the oxidation of various inorganic and organic compounds such as phenolic compounds, diamine, aromatic amines and ascorbic acid (Mayer and Staples, 2002). Laccase enzyme was first isolated by Yoshida in 1883. He extracted from the Japanese lacquer tree (Rhus vernicifera) (Thurston, 1994; Risdianto, 2008 ; Shraddha dkk., 2011). Objectives of this study is to determine the commercial activity of the enzyme laccase derived from Rhus vernicifera using guaiacol as a substrate. The enzyme activity was measured using a UV-Vis spectrophotometer Thermo Scientific GENESYS 10S at a wavelength of 490 nm using guaiacol as substrate. The results of this study indicate that this enzyme has an activity of 0,0247 ± 0,0002 U/L (at pH 5,5 and a temperature of 40 oC).
Co-Authors