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Infection of Chicken Anaemia Virus: Etiology, Epidemiology, Clinical Sign, Pathological Changes and Disease Control Wahyuwardani, Sutiastuti; Syafriati, Tatty
Indonesian Bulletin of Animal and Veterinary Sciences Vol 15, No 3 (2005)
Publisher : Indonesian Animal Sciences Society

Initially, Chicken anaemia virus (CAV) is known as CAA, which was first isolated by Yuasa in Japan in 1976. CAV particles are non enveloped with a diameter of 19 .1-20 .7nm, belonging to the family Circoviridae, genus Gyrovirus . CAV infection was first appeared in Indonesia at the same time as the outbreak of stunting and runting syndrome in 1996 with a mortality rate of 5-15% but it may reach to 60%. CAV can be transmitted vertically or horizontally. Chicken all ages is susceptible to infection. Infection of CAV occurred in young chicken flock at 2-3 weeks growth of age, causing clinical signs while in old chicken flock which is sub clinical. The signs of infectious of CAV are retarded, anaemia, anorexia, pale of face and pial. The pathology anatomy changes are pale carcases, yellowish bone marrow, atrophy of thymus and bursa fabricius . Whereas, histophatological changes are thymic necrosis of cortex and medulla, lymphocyte depletion of thymus, bursa fabricius and bone marrow. Diagnose of CAV is based on pathological changes and followed by the isolation of certain lymphoblastoid chicken cell MDCC-MSB I and then, is identified by virus neutralization. The presence of virus can also be identified by immunofluorescent and immunoperoxidase staining, in situ hybridization technique or PCR. For antibody detection, ELISA technique can be used. The syndromes of CAV infection are closely associated with those of osteopetrosis, reovirus, infectious bursal disease (IBD) and Marek. Vaccination programme in breeding farm is needed to induce maternal antibody. This paper describes the CAV disease and its occurrence in Indonesia. Key word : Chicken anaemia virus, diagnose, pathology anatomy, histopathology, control

Infection of Parainfluenza type 3 (PI-3) as one of the causative agent of pneumonia in sheep and goats Sendow, Indrawati; Syafriati, Tatty; Wiedosari, Ening; Selleck, Paul
Indonesian Journal of Animal and Veterinary Sciences Vol 7, No 1 (2002)
Publisher : Indonesian Animal Sciences Society

Serological survey was conducted to obtain the prevalence of Parainfluenza type 3 (PI-3) reactor as one of the causative agent of pneumonia in sheep and goats in abatoir at Jakarta and some small holder farms in Indonesia. Serological test using serum neutralization from 724 goat sera and 109 sheep sera indicated that only 1% of goats were serologically reactors and none of sheep sera had antibodies against PI-3 virus. Isolation of the virus from 56 bronchus and trachea swab and 345 lungs indicated that only one sampel from lung showed cythopathic effect (CPE) in Madin Darby Bovine Kidney (MDBK) cell lines identification of the virus using serum neutralization test indicated that the virus neutralized reference PI-3 antisera. The isolate came from one lung (7%) of 24 that showed histopathologically pneumonia intertitialis that usually caused by viral infection. Key words: Parainfluenza type 3 (PI-3), virus isolation, sheep, goat, pneumonia intertisialis

Epidemiology of JapaneseâBâ encephalitis infection in pigs in Riau and North Sumatera Provinces Sendow, Indrawati; Syafriati, Tatty; Hadi, Upik Kesumawati; Malole, Martin; Soviana, Susi; ., Darminto
Indonesian Journal of Animal and Veterinary Sciences Vol 8, No 1 (2003)
Publisher : Indonesian Animal Sciences Society

Epidemiology study on Japanese-B-Encephalitis (JE) was conducted in Riau and North Sumatera Provinces. A total of 190 pig sera from Riau Province and 164 pig sera from North Sumatera were tested using competitive ELISA (C-ELISA) to detect antibodies against JE virus. Insect collection was also conducted using several methods near pig farms in those provinces and identified into species to gain more information on its role to distribute JE infection. Serological results indicated that 70% pig in Sumatera and 94% pig in Riau had antibodies against JE virus. The highest prevalence of reaktor was detected in pig of more than 4 months age in both Provinces. The results of insect collection showed that Culex tritaeniorchynchus and Culex quinquefasciatus were the most dominant species in both provinces. Based on serological testing, indicated that JE virus infected pig in Sumatera and Riau Provinces, and higher reactor was obtained in older pig. Culex tritaeniorchynchus and Culex quinquefasciatus were the dominant insect species in both provinces, hence those species had a possibility to play an important role of JE transmission. Â Key words: JE, pigs, serology, insects

Seroepidemiology of Canine parvovirus infection in dogs Sendow, Indrawati; Syafriati, Tatty
Indonesian Journal of Animal and Veterinary Sciences Vol 9, No 3 (2004)
Publisher : Indonesian Animal Sciences Society

Canine parvovirus is an acute and fatal viral disease in dogs. A total of 209 local, cross breed and breed dogs sera from Kodya Bogor, Kabupaten Bogor, Sukabumi, and Jakarta, had been tested using Haemagglutination Inhibition Test (HI) with pig red blood cells. A total of 64 breed and cross breed dogs from Sukabumi and Kodya Bogor, were used as a sentinel dogs to study the epidemiology of Canine parvovirus (CPV) infection and its immunological responses caused by vaccination. The results indicated that 78% (95) breed and cross bred dogs and 59% (51) local dogs had antibody to CPV. Sentinel dogs results indicated that dogs had been vaccinated showed antibody response with the varied titre dependant upon prevaccination titre. Low prevaccinated titre gave better response than protective level titre. From 19 puppies observed, Maternal antibodi were still detected until 5 weeks old puppies. First vaccination given at less than 3 months old, should be boosted after 3 months old puppied. Antibodi titre produced by natural infection will keep untill 2 years. These data concluded that the dog condition and time of vaccination will affect the optimum antibody response. Â Key words: Canine parvovirus, haemagglutination inhibition, isolation

Gambaran seroepidemiologi dan histopatologi infeksi virus parainfluenza tipe 3 pada sapi Sendow, Indrawati; Syafriati, Tatty; Damayanti, Rini
Indonesian Journal of Animal and Veterinary Sciences Vol 9, No 2 (2004)
Publisher : Indonesian Animal Sciences Society

A study to gain seroepidemiological feature and histopathological changes in order to obtain a viral causative agent had been conducted against parainfluenza virus type 3 (PI-3) in infection cattle in Indonesia. Serological survey was conducted in different areas in Indonesia and from serum Bank to gain the information on the distribution of parainfluenza type 3 (PI-3) in large ruminants. A total of 1334 sera had been tested using serum neutralization test, and the result indicated that prevalence of reactors was varied from 0 to 60 %. The highest prevalence was 60% in sera detected from Bogor abbatoir. Reactors were also found in other areas such as West Java, Central Java, East Java, NTT and Papua. Titration results indicated that the distribution of titre was varied from 4 to 256, and titre of 8 to 32 was the most common. Titre of 128 and 256 was only found in each of 1 sera only. Isolation results indicated that no isolate was obtained from 237 samples processed. Histological examination showed that more than 60% had interstitial pneumonia, which indicated vairal infection had been occurred. This serological result indicated that PI-3 infection was detected in Indonesian large ruminants. Â Key words: Parainfluenza type 3 virus, serology, histopathology

Serological study against transmissible gastroenteritis (TGE) virus in several area in Indonesia Sendow, Indrawati; Syafriati, Tatty; Bahri, Sjamsul; Sarosa, Antonius
Indonesian Journal of Animal and Veterinary Sciences Vol 3, No 3 (1998)
Publisher : Indonesian Animal Sciences Society

A number of 1,168 pig and dog sera from 8 provinces in Indonesia were tested serologically for transmissible gastroenteritis (TGE) antibodies using serum neutralisation test to detect the prevalence of TGE in Indonesia. The sera were obtained from serum bank at Research Institute for Veterinary Science, Bogor. All sera collected before 1995 were negative antibody to TGE. However, sera collected from 2 provinces Sumatera Utara and Sulawesi Utara in 1996 had antibodies against TGE virus (14.03%). Titration of reacted sera showed varied between titres of 8 to 128. Â Key words: Transmissible gastroenteritis virus, serum neutralization test

Seroepidemiologi Nipah Virus pada Kalong dan Ternak Babi di Beberapa Wilayah di Indonesia Sendow, Indrawati; Field, Hume; Adjid, R.M. Abdul; Syafriati, Tatty; Darminto, Darminto; Morrissy, Chris; Daniels, Peter
JURNAL BIOLOGI INDONESIA Vol 5, No 1 (2008): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

ABSTRACTNipah Virus Seroepidemiology in Flying Fox and Pig Husbandry in Several Areasof Indonesia. Nipah is a dangerous zoonotic disease which was carried by flying fox.The disease had been occurred in Malaysia in 1999 and infect pigs and caused humandeath. Indonesia is adjacent country to Malaysia, hence, a serological study had beenconducted on 156 flying fox (P. vampyrus) sera from North Sumatera, West Java, CentralJava and East Java. Besides that, 2740 pig sera was randomly collected in differentprovinces to detect Nipah infection. Both flying fox and pig sera were tested usingELISA test to detect the presence of Nipah antibody. The results indicated that 37 from156 flying fox sera (23.7%) has antibodies against Nipah virus. Infections were occuredin all sampling sites with the prevalence varied from 18% to 33 %. Meanwhile, no pigsera tested (2740) had antibody against Nipah virus. Based on these results it can beconcluded that Nipah virus infections were occurred in flying fox in some parts inIndonesia, but not in pigs. It was suggested that the presence of Nipah virus in Indonesiashould be anticipated. Hence the distribution of its infection in pigs and human must beanticipated. Monitoring of Nipah infection in areas adjacent to Malaysia must be increasedto detect the entering of the disease in Indonesia.Keywords: Nipah, pigs, flying fox, serology

SEROEPIDEMIOLOGI NIPAH VIRUS PADA KALONG DAN TERNAK BABI DI BEBERAPA WILAYAH DI INDONESIA Sendow, Indrawati; Field, Hume; Adjid, R.M. Abdul; Syafriati, Tatty; Darminto, Darminto; Morrissy, Chris; Daniels, Peter
JURNAL BIOLOGI INDONESIA Vol 5, No 1 (2008): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v5i1.3205

ABSTRACTNipah Virus Seroepidemiology in Flying Fox and Pig Husbandry in Several Areasof Indonesia. Nipah is a dangerous zoonotic disease which was carried by flying fox.The disease had been occurred in Malaysia in 1999 and infect pigs and caused humandeath. Indonesia is adjacent country to Malaysia, hence, a serological study had beenconducted on 156 flying fox (P. vampyrus) sera from North Sumatera, West Java, CentralJava and East Java. Besides that, 2740 pig sera was randomly collected in differentprovinces to detect Nipah infection. Both flying fox and pig sera were tested usingELISA test to detect the presence of Nipah antibody. The results indicated that 37 from156 flying fox sera (23.7%) has antibodies against Nipah virus. Infections were occuredin all sampling sites with the prevalence varied from 18% to 33 %. Meanwhile, no pigsera tested (2740) had antibody against Nipah virus. Based on these results it can beconcluded that Nipah virus infections were occurred in flying fox in some parts inIndonesia, but not in pigs. It was suggested that the presence of Nipah virus in Indonesiashould be anticipated. Hence the distribution of its infection in pigs and human must beanticipated. Monitoring of Nipah infection in areas adjacent to Malaysia must be increasedto detect the entering of the disease in Indonesia.Keywords: Nipah, pigs, flying fox, serology

Sebuah pengamatan terhadap dinamika populasi sapi Bali di Bali Soeharsono .; D.H.A. Unruh; I Gde Sudana; M. Gunawan; Dewa N. Dharma; A.A. Gde Putra; Tatty Syafriati; Slamet Witono; I Gde Kartayadnya; Ketut Santhia; Adhy Putra; G.M. Alit Putra; M. Malole
Hemera Zoa Vol. 71 No. 2 (1983): Jurnal Hemera Zoa
Publisher : Hemera Zoa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (556.441 KB)

Suatu pengamatan terhadap sapi Bali di Bali telah dilakukan pada bulan Juni 1980 sampai dengan bulan Mei 1981.Tujuan utama dari pengamatan ini adalh untuk memperoleh data dasar mengenai produksi dan kehilangan (kematian dsb.) pada populasi.Hasil yang diperoleh menggambarkan umur, struktur, angka kematian, angka kelahiran, angka keguguran dan angka pemindahan pada sapi betina. Data ini dapat dipakai untuk menaksir kerugian yang disebabkan oleh kematian dan menyelidiki penurunan populasi.

Gambaran seroepidemiologi dan histopatologi infeksi virus parainfluenza tipe 3 pada sapi Indrawati Sendow; Tatty Syafriati; Rini Damayanti
Jurnal Ilmu Ternak dan Veteriner Vol 9, No 2 (2004): JUNE 2004
Publisher : Indonesian Center for Animal Research and Development (ICARD)

A study to gain seroepidemiological feature and histopathological changes in order to obtain a viral causative agent had been conducted against parainfluenza virus type 3 (PI-3) in infection cattle in Indonesia. Serological survey was conducted in different areas in Indonesia and from serum Bank to gain the information on the distribution of parainfluenza type 3 (PI-3) in large ruminants. A total of 1334 sera had been tested using serum neutralization test, and the result indicated that prevalence of reactors was varied from 0 to 60 %. The highest prevalence was 60% in sera detected from Bogor abbatoir. Reactors were also found in other areas such as West Java, Central Java, East Java, NTT and Papua. Titration results indicated that the distribution of titre was varied from 4 to 256, and titre of 8 to 32 was the most common. Titre of 128 and 256 was only found in each of 1 sera only. Isolation results indicated that no isolate was obtained from 237 samples processed. Histological examination showed that more than 60% had interstitial pneumonia, which indicated vairal infection had been occurred. This serological result indicated that PI-3 infection was detected in Indonesian large ruminants. Key words: Parainfluenza type 3 virus, serology, histopathology

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