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All Journal Jurnal Ilmu Pertanian Indonesia Jurnal Teknologi Industri Pertanian E-jurnal Agro-Industri Indonesia Forum Pasca Sarjana International Journal of Science and Engineering (IJSE) International Journal of Renewable Energy Development TEKNIK Teknotan: Jurnal Industri Teknologi Pertanian Jurnal Sains Materi Indonesia Microbiology Indonesia Majalah Kedokteran Bandung BIOTROPIA - The Southeast Asian Journal of Tropical Biology AL KAUNIYAH Agrointek Jurnal AgroBiogen Jurnal Penelitian Pascapanen Pertanian Jurnal Teknologi Pertanian Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) Jurnal Litbang Industri Register: Jurnal Ilmiah Teknologi Sistem Informasi Jurnal Bioteknologi & Biosains Indonesia (JBBI) EDUFORTECH IJEMS (Indonesian Journal of Environmental Management and Sustainability) Seminar Nasional Lahan Suboptimal Menara Perkebunan Teknologi Indonesia STI Policy and Management Journal JURNAL ILMU KEFARMASIAN INDONESIA PERENNIAL Wood Research Journal : Journal of Indonesian Wood Research Society Wood Research Journal : Journal of Indonesian Wood Research Society Jurnal Litbang Industri Makara Journal of Science Makara Journal of Technology
Khaswar Syamsu
Bogor Agriculture University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Electrical & Electronics Engineering Energy Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Public Health Veterinary Other

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The Fermentation Study on Polyhydroxyalkanoates Produced by Ralstonia eutropha from Hydrolized Sago Starch as The Carbon Source Atifah, Nur; Syamsu, Khaswar; Suryani, Ani
Jurnal Teknologi Pertanian Vol 8, No 3 (2007)
Publisher : Fakultas Teknologi Pertanian Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (281.987 KB)

Abstract

Polyhydroxyalkanoates (PHAs), microbial bioplastics, not only were similar in properties with those made of petrochemical products, they were also completely biodegradable. At the moment, the price of PHAs was still high. This research was conducted to investigate the ability of Ralstonia eutropha to produce PHAs on a sago starch-based substrate, one of the cheap and abundantly renewable resources in Indonesia, and to characterize the PHAs produced. The microorganism was grown on the hydrolyzed sago starch with an initial sugar concentration of 30 g/lIt was found that the best value of the maximum specific growth rate (µmax) was  0,188 h-1. Kinetic parameters at 96 hour-batch fermentation showed the respective final cell and PHA concentrations of 4,41 g/L and 1,44 g/L. The yields of cell biomass (Yx/s), PHAs per gram of cells (Yp/x), PHAs per gram of substrate (Yp/s) and the percentage of substrate consumption  (∆S/So) were, 0.15 g cell/g sugar; 0.35 g PHA/g cells; 0.06 g PHA/g sugar and 99%, respectively.  The PHAs produced were predominantly composed of poly(3-hydroxybutyrate) (PHB) as the functional groups with a melting point of 163,96 oC.Keywords : hydrolyzed sago starch, PHA, Ralstonia eutropha
Penentuan Teknologi Proses Pembuatan Gambir Murni dan Katekin Terstandar dari Gambir Asalan Yeni, Gustri; Syamsu, Khaswar; Mardliyati, Etik; Muchtar, Hendri
Jurnal Litbang Industri Vol 7, No 1 (2017)
Publisher : Institution for Industrial Research and Standardization of Industry - Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (528.758 KB) | DOI: 10.24960/jli.v7i1.2846.1-10

Abstract

Uncaria gambir Roxb. is an industrial crop commodity that has a high economic value and good prospect for farmers and exporter. Gambier of traditional processed products (raw gambier) generally have various catechin content, so it is required further handling to increase the purity of catechins from raw gambier. The research was aimed to get a process technology on the making of pure gambier and standardized catechins. Sources of raw materials was obtained from extraction process through steaming leaves and twigs of gambier (KA) using equipment from aluminum (RA) and from iron (RF). Purification of raw gambier through repeated extraction with water could decrease tannin content from 24% to 2.4% and increase catechin levels between 40% to 74%. Gambier with a low tannin content (catechin KA) through re-extraction using water had the highest increase of catechin content with a color of yellowish white product. The iron-containing equipment affected the color of the pure gambier produced, which was reddish-brown. The effect of solvent on further extraction using ethyl acetate solvent resulted in higher catechin content (up to 99%) compare to ethanol (95%). The catechin purity of KA samples was tested by looking at its stability at varying levels of acidity, showing catechins of gambier was stabile at pH 6.ABSTRAK Uncaria gambir Roxb. merupakan komoditas tanaman industri yang memiliki nilai ekonomi tinggi serta mempunyai prospek cukup baik bagi petani dan pemasok negara-negara asing. Gambir hasil olahan tradisional (gambir asalan), umumnya memiliki kandungan katekin yang beragam, sehingga diperlukan penanganan lebih lanjut untuk meningkatkan kemurnian katekin dari gambir asalan. Tujuan penelitian adalah untuk mendapatkan teknologi proses pembuatan gambir murni dan katekin terstandar. Sumber bahan baku diperoleh dari proses ekstraksi melalui pengukusan daun dan ranting tanaman gambir (KA) menggunakan peralatan dari aluminium (RA) dan dari besi (RF). Pemurnian gambir asalan melalui ekstraksi berulang dengan air dapat menurunkan kadar tanin, yaitu dari 24% sampai 2,4% dan meningkatkan kadar katekin, yaitu antara 40% sampai 74%. Gambir dengan kandungan tanin rendah (katekin KA) melalui ekstraksi ulang menggunakan air memiliki peningkatan kadar katekin tertinggi dengan warna produk putih kekuningan. Peralatan mengandung besi berpengaruh terhadap warna dari gambir murni yang dihasilkan, yaitu berwarna coklat kemerahan. Pengaruh pelarut terhadap ekstraksi lanjut menggunakan pelarut etil asetat menghasilkan kadar katekin lebih tinggi (sampai 98%) dibandingkan etanol (95%). Kemurnian katekin sampel KA diuji dengan melihat stabilitasnya pada berbagai tingkat keasaman, menunjukkan katekin dari gambir stabil pada pH 6.
Penentuan Kondisi Terbaik Ekstraksi Antioksidan dari Gambir Menggunakan Metode Permukaan Respon Yeni, Gustri; Sa’id, E. Gumbira; Syamsu, Khaswar; Mardliyati, Etik
Jurnal Litbang Industri Vol 4, No 1 (2014)
Publisher : Institution for Industrial Research and Standardization of Industry - Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1403.273 KB) | DOI: 10.24960/jli.v4i1.637.39-48

Abstract

Extract of Uncaria gambier contains phenolic compounds that have function as an antioxidants. Antioxidant content depends on the extraction conditions. To obtain high antioxidant activity of the phenolic compounds, the optimization process was carried out using response surface methodology (RSM) with three variables namely  the ratio of solvent (ethyl acetat : ethanol), the ratio of sample : solvent and time of extraction with the antioxidant activity DPPH (IC50) as response. Optimization result showed a quadratic polynomial regression equation of Y = 137.25 + 5.18 (R.solvent) + 24.75 (R.sample) + 11.53 (Time of.extrac) + 6.17 (R.solvent x sample) + 3.14 (Rsolvent x Time of.extrac) + 13.79 (R.sample x Time of.extrac) – 5.44 (R.solvent2) – 15.33 (R.sample2) – 5.42 (Time of.extrac2). The optimum response was achieved at the solvent ratio of ethyl acetat : ethanol = 0,37 : 0,55, ratio of sample : solvent = 1:2 and time of extraction 20 hours.  Validation result revealed that the predicted value (IC50 = 82.89 mg/mL) of this model was reasonably close to the experimental result (IC50 = 82,735 ± 0,362 mg/mL). Visual characterization of the extracts indicated that the extracts have different colour in each treatment, depending on the ratio of the solvent used.ABSTRAK Ekstrak dari Uncaria gambir mengandung senyawa fenolik katekin yang berfungsi sebagai antioksidan. Kandungannya pada hasil ekstrak bergantung pada kondisi ekstraksi yang digunakan. Untuk mendapatkan kondisi ekstraksi terbaik senyawa fenolik dari gambir dengan aktivitas antioksidan yang tinggi dilakukan proses optimasi menggunakan metode permukaan respon (RSM) pada tiga variabel yaitu rasio pelarut, rasio sampel dengan pelarut dan lama ekstraksi dengan respons aktivitas antioksidan terhadap antiradikal DPPH (IC50). Hasil optimasi menunjukkan persamaan kuadrat polinomial regresi yaitu Y = 137,25 + 5,18 (Rpelarut) + 24,75 (Rsampel) + 11,53 (Lamaeks) + 6,17 (Rpelarut x sampel) + 3,14 (Rpelarut x lamaeks) + 13,79 (Rsampel x lamaeks) - 5,44 (Rpelarut2) - 15,33 (Rsampel2) - 5,42 (Lamaeks2). Kondisi optimum dicapai pada rasio etil asetat dengan etanol  0,37:0,55, rasio sampel dengan pelarut 1:2 dan lama ekstraksi 20 jam. Hasil validasi menunjukkan nilai prediksi (IC50 = 82.89 mg/ml) dari model tidak berbeda signifikan dengan nilai hasil validasi (IC50= 82,735 ± 0,362 mg/ml). Karakteristik produk hasil ekstraksi secara visual menunjukkan warna yang berbeda dari setiap perlakuan dan tergantung dari rasio pelarut yang digunakan.
PENINGKATAN PRODUKSI SEFALOSPORIN C DARI Acremonium chrysogenum CB2/11/1.10.6 DENGAN OPTIMASI MEDIA MENGGUNAKAN METODE RESPON PERMUKAAN Prabandari, Erwahyuni; Hidayati, Dyah Noor; Dewi, Diana; Islamiati, Eni Dwi; Syamsu, Khaswar
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol 4, No 1 (2017): June 2017
Publisher : Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (816.471 KB) | DOI: 10.29122/jbbi.v4i1.1808

Abstract

Cephalosporin is a β-lactam antibiotic produced by Acremonium chrysogenum using submerged fermentation. Carbon and nitrogen are the most influential medium ingredients for cephalosporin formation. The purpose of this study was to obtain the best composition of media for cephalosporin C production. Response surface methodology was used for production optimization. The results showed that molasses of 70 g/Lwas the best carbon source, while the best nitrogen source was the combination of corn steep liquor, urea and ammonium sulphate. DL-methionine, carbon, and nitrogen source significantly affected  the production of cephalosporin C. The mathematically modelled optimization showed that the highest production of cephalosporin C (3876 mg/L) was obtained using medium composition of 68.28 g/L molasses, 71.61 g/L nitrogen, and 0.4 g/L DL-methionine. Laboratory verification using the same medium composition produced 3696 mg/L of cephalosporin C, being 4.65% different from the mathematically optimized results. Medium optimization increased the cephalosprin C production which was 1.48 times higher than that using the previous medium, where the maximum production was only 2487 mg / L.Keywords: Carbon,  cephalosporin C, cultivation medium, nitrogen, A. chrysogenum ABSTRAKSefalosporin C adalah golongan antibiotik β-lactam yang dihasilkan Acremonium chrysogenum melalui fermentasi cair. Komponen yang sangat berpengaruh terhadap produksi sefalosporin C adalah sumber karbon dan nitrogen. Penelitian ini bertujuan mendapatkan komposisi media terbaik untuk produksi sefalosporin C. Optimasi dilakukan menggunakan metode respon permukaan. Hasil menunjukkan bahwa molases 70 g/L adalah sumber karbon terbaik dan kombinasi corn steep liquor, urea dan ammonium sulfat adalah sumber nitrogen terbaik. DL-methionin, sumber karbon, dan nitrogen berpengaruh nyata terhadap produksi sefalosporin C. Optimasi menggunakan model matematika menunjukkan produksi sefalosporin C tertinggi (3876 mg/L) yang diperoleh dengan komposisi media 68,28 g/L molases, 71,61 g/L nitrogen, dan  0,4 g/L DL-methionin. Verfikasi di laboratorium menggunakan komposisi media yang sama menghasilkan sefalosporin C sebesar 3696 mg/L, berbeda 4,65% dibanding dengan hasil optimasi matematis. Optimasi media mampu meningkatkan produksi sefalosprin C sebesar 1,48 kali dibanding media yang digunakan sebelumnya, dimana maksimal hanya menghasilkan 2487 mg/L.Kata kunci: Karbon, sefalosporin C, media kultivasi, nitrogen, A. chrysogenum
PERFORMANCE OF MICROWAVE PRETREATMENT ON ENZYMATIC AND MICROWAVE HYDROLYSIS OF BETUNG BAMBOO (Dendrocalamus asper) Fatriasari, Widya; Syafii, Wasrin; Wistara, Nyoman; Syamsu, Khaswar; Prasetya, Bambang
Teknologi Indonesia Vol 37, No 3 (2014)
Publisher : LIPI Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jti.v37i3.232

Abstract

In this present study, we focused on how the changes in the best microwave pretreatment affected the performance of enzymatic and microwave hydrolysis of pretreated bamboo. This solid fraction was subjected to enzymatic hydrolysis following NRE L protocol and microwave acid-hydrolysis with/without presence of activated carbon (0.5 g/g) at 1% and 5% of sulfuric acid concentration for 512.5 m at 330 watt. Short duration microwave-acid hydrolysis of microwave pretreated bamboo showed better reducing sugar yield than that of enzymatic one. Microwave heating for 12.5 inm at 330 W hydrolyzed with 1% acid using microwave irradiation demonstrated a high reducing sugar yield (25.81% of dry biomass or 27.12% of dry substrate). In this treatment, 37.92% of hollocellulose can be converted into reducing sugar or equivalent to 36.12% of maximum potential sugar released. This yield was increased 6.09 times compared to the highest reducing sugar yield coming from enzymatic hydrolysis using 20 FPU/g of cellulase enzymes. Reducing sugar yield obtained with 5% microwave acid hydrolysis of microwave pretreatment for 5 m at 770 W was equal to the result of hydrolysis for 12.5 m at 330 W. Unfortunately, the activated carbon as solid catalyst in microwave hydrolysis could only reduce brown compound without reducing sugar yield improvement.
Optimization of Penicillium Lagena Medium Cultivication on Antifungal Pathogen of Phellinus Lamaoensis Using Surface Methode Nabilah, Siti; Sunaryanto, Rofiq; Syamsu, Khaswar
TEKNIK Vol 38, No 2 (2017): (Desember 2017)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (368.107 KB) | DOI: 10.14710/teknik.v38i2.10306

Abstract

Phellinus lamaoensis (Murr.) Hein is fungal pathogen that can cause brown root rot disease in cocoa, tea, rubber, and coffee plants. Endophytic fungi, Penicillium lagena, isolated from bandotan (Ageratum conyzoides Linn.), medicinal plant, is able to inhibit the growth of pathogenic, P. lamaoensis. The effect of carbon source, nitrogen source, and mineral solution was studied. Lactose, yeast extract, and mineral solution were media components which showed significant effect toward production of P. lagena active compound. Composition optimization of these three medium components was done by response surface methodology (RSM). The Optimal response region of the significant factor was predicted by using a second order polynomial model with statistical design, central composite design (CCD). Higest production of P. lagena active compound by quadratic model was predicted to be 69.233%  with medium composition 44.77 g L-1 lactose, 13.02 g L-1 yeast extract, and 15.95 mL L-1 mineral solution. Verification value in laboratory is 58.365%, lower 15.7% than its prediction. Optimization increase P. lagena active compound 9 fold compared to unoptimize media.
PENINGKATAN PRODUKSI SEFALOSPORIN C DARI Acremonium chrysogenum CB2/11/1.10.6 DENGAN OPTIMASI MEDIA MENGGUNAKAN METODE RESPON PERMUKAAN Prabandari, Erwahyuni; Hidayati, Dyah Noor; Dewi, Diana; Islamiati, Eni Dwi; Syamsu, Khaswar
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 4 No. 1 (2017): June 2017
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (816.471 KB) | DOI: 10.29122/jbbi.v4i1.1808

Abstract

Cephalosporin is a β-lactam antibiotic produced by Acremonium chrysogenum using submerged fermentation. Carbon and nitrogen are the most influential medium ingredients for cephalosporin formation. The purpose of this study was to obtain the best composition of media for cephalosporin C production. Response surface methodology was used for production optimization. The results showed that molasses of 70 g/Lwas the best carbon source, while the best nitrogen source was the combination of corn steep liquor, urea and ammonium sulphate. DL-methionine, carbon, and nitrogen source significantly affected  the production of cephalosporin C. The mathematically modelled optimization showed that the highest production of cephalosporin C (3876 mg/L) was obtained using medium composition of 68.28 g/L molasses, 71.61 g/L nitrogen, and 0.4 g/L DL-methionine. Laboratory verification using the same medium composition produced 3696 mg/L of cephalosporin C, being 4.65% different from the mathematically optimized results. Medium optimization increased the cephalosprin C production which was 1.48 times higher than that using the previous medium, where the maximum production was only 2487 mg / L.Keywords: Carbon,  cephalosporin C, cultivation medium, nitrogen, A. chrysogenum ABSTRAKSefalosporin C adalah golongan antibiotik β-lactam yang dihasilkan Acremonium chrysogenum melalui fermentasi cair. Komponen yang sangat berpengaruh terhadap produksi sefalosporin C adalah sumber karbon dan nitrogen. Penelitian ini bertujuan mendapatkan komposisi media terbaik untuk produksi sefalosporin C. Optimasi dilakukan menggunakan metode respon permukaan. Hasil menunjukkan bahwa molases 70 g/L adalah sumber karbon terbaik dan kombinasi corn steep liquor, urea dan ammonium sulfat adalah sumber nitrogen terbaik. DL-methionin, sumber karbon, dan nitrogen berpengaruh nyata terhadap produksi sefalosporin C. Optimasi menggunakan model matematika menunjukkan produksi sefalosporin C tertinggi (3876 mg/L) yang diperoleh dengan komposisi media 68,28 g/L molases, 71,61 g/L nitrogen, dan  0,4 g/L DL-methionin. Verfikasi di laboratorium menggunakan komposisi media yang sama menghasilkan sefalosporin C sebesar 3696 mg/L, berbeda 4,65% dibanding dengan hasil optimasi matematis. Optimasi media mampu meningkatkan produksi sefalosprin C sebesar 1,48 kali dibanding media yang digunakan sebelumnya, dimana maksimal hanya menghasilkan 2487 mg/L.Kata kunci: Karbon, sefalosporin C, media kultivasi, nitrogen, A. chrysogenum
PENGARUH TEPUNG TERIPANG PASIR (Holothuria scabra) TERHADAP PERILAKU SEKSUAL DAN KADAR TESTOSTERON DARAH MENCIT (Mus musculus) Nurjanah, Sarifah; Gumbira-Sa'id, E; Syamsu, Khaswar; Suprihatin, Suprihatin; Riani, Etty
Majalah Kedokteran Bandung Vol 41, No 3
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Masyarakat meyakini teripang dapat dijadikan makanan yang dapat meningkatkan vitalitas laki-laki. Penelitian ini bertujuan untuk mengkaji potensi teripang sebagai aprodisiaka dengan mempelajari pengaruh tepung teripang pasir terhadap perilaku seksual mencit jantan. Metode yang digunakan dalam penelitian ini adalah metode eksperimental laboratories. Perlakuan yang dikaji adalah pemberian tepung teripang pada tingkat dosis kandungan steroid 10, 30 dan 50 ìg steroid/100 g bobot badan mencit jantan, sebagai kontrol digunakan perlakuan tanpa pemberian hormon dan pemberian metil testosteron. Pemberian ekstrak dilakukan selama 12 hari. Pengamatan yang dilakukan meliputi perilaku seksual dan kadar testosteron darah mencit. Perilaku seksual mencit jantan yang diamati meliputi aktifitas kissing vagina dan mounting, yang dilakukan satu jam setelah pemberian ekstrak. Hasil penelitian menunjukkan bahwa pemberian tepung teripang dapat meningkatkan frekuensi perilaku seksual mencit. Hal tersebut ditunjukkan oleh meningkatnya frekuensi perilaku kissing vagina dan mounting dibandingkan dengan kontrol. Frekuensi perilaku seksual tertinggi ditunjukkan oleh mencit yang diberi tepung teripang pada konsentrasi kandungan steroid 10 ìg /100 g bobot badan dengan frekuensi kissing vagina 25 kali dalam 30 menit dan mounting 6 kali dalam 30 menit. Pemberian tepung teripang juga dapat meningkatkan kadar testosteron dalam darah mencit percobaan. Hal ini diduga karena tepung teripang mengandung steroid juga mengandung sejumlah nutrisi yang sangat berguna dalam peningkatan libido. Hasil penelitian membuktikan bahwa tepung teripang berpotensi sebagaiaprodisiaka alami.Kata kunci: tepung teripang, perilaku seksual, kadar testosteronEFFECT OF SANDFISH (HOLOTHURIA SCABRA) POWDER ON THE SEXUAL BEHAVIOR AND THE BLOOD TESTOSTERONE LEVEL OF THE MALE MOUSE (MUS MUSCULUS)Sea cucumber is generally believed as a natural material that can be used as a tonic food to increase man vitality. The aim of this study was to investigate the effect of sandfish powder on sexual behavior and blood testosterone level of male mice. Method applied in the study was laboratory experimental method. Mature male mice were treated with administration of sandfish powder with three dosage rate of steroid content (10, 30 and 50 ìg/100 g body weight) during 12 days, whereas for control treatment were without hormone administration and with the metil testosterone administration. Parameters that were investigated were kissing vagina and mounting for sexual behavior and the blood testosterone level of male mice. It was found that administration of sandfish powder significantly give effect on the number of kissing vagina and mounting compared to control. Administration of 10 ìg/100 g body weight on male mice showed the highest sexual behavior with 25 kissing vagina for and 6 mounting for 30 minutes. Moreover, administration of sandfish powder increased the testosterone level in the male mice blood. This may due to the steroid contained in sandfish powder and nutrition value that increase mice libido. The study proved that the sandfish powder has a potential as a nature aphrodisiac.Keywords: sandfish powder, sexual behavior, testosterone level DOI: http://dx.doi.org/10.15395/mkb.v41n3.247
Purifikasi dan Karakterisasi α-amilase Termostabil dari Bacillus stearothermophilus TII-12 Lestari, Puji; Richana, Nur; Darwis, Abdul Aziz; Syamsu, Khaswar; Murdiyatmo, Untung
Jurnal AgroBiogen Vol 7, No 1 (2011): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v7n1.2011.p56-62

Abstract

Purification and Characterization of Thermostableα-amylase from Bacillus stearothermophilus TII-12. PujiLestari, Nur Richana, Abdul A. Darwis, Khaswar Syamsu,and Untung Murdiyatmo. Thermostable α-amylase is apotential enzyme employed in the starch processing andwidely used in food industries, but this enzyme is stillimported. The local enzyme production would be moreeconomist and useful for its broad applications. Here wereport α-amylase from indigenous bacteria TII-12 which waspurified and characterized, as well as analyzed its hydrolysisproduct on cassava starch. The enzyme of Bacillusstearothermophilus TII-12 partially purified by ultrafiltration,acetone precipitation and gel filtration (Sephadex G-100)showed the reduced total activity, total protein and yield, butincreased the specific activity. The enzyme had a Km of 1,06mg/ml and Vmax of 1,21 mol/min, with optimal activity at pH 7and 90oC. An apparent molecular mass was of 192.932,8Dalton, as estimated by Native-Polyacrylamide Agarose Gelelectrophoresis. Its activity was inhibited by the divalentcation chelator such as EDTA and CuSO4 but activated bycalcium ion. Hydrolysis products of this enzyme on cassavastarch were glucose, dextrin, maltose and oligosaccharides.After 24 hours of hydrolysis, the concentration of glucoseand maltose reached 51.970 and 10.090 ppm, respectively.The thermostable α-amylase of TII-12 is an endo-α-amylaseand prospective to be applied on starch liquefaction withhigh temperature process.
The characteristic changes of betung bamboo (Dendrocalamus asper) pretreated by fungal pretreatment Fatriasari, Widya; Syafii, Wasrin; J Wistara, Nyoman; Syamsu, Khaswar; Prasetya, Bambang
International Journal of Renewable Energy Development Vol 3, No 2 (2014): July 2014
Publisher : Center of Biomass & Renewable Energy, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ijred.3.2.133-143

Abstract

The fungal pretreatment effect on chemical structural and morphological changes of Betung Bamboo was evaluated based on its biomass components after being cultivated by white rot fungi, Trametes versicolor. Betung bamboo powder (15 g) was exposed to liquid inoculum of white rot fungi and incubated at 270C for 15, 30 and 45 days. The treated samples were then characterized by FT-IR spectroscopy, X-Ray diffraction and SEM-EDS analyses. Cultivation for 30 days with 5 and 10% loadings retained greater selectivity compared to that of the other treatments. FTIR spectra demonstrated that the fungus affected the decreasing of functional group quantities without changing the functional groups. The decrease in intensity at wave number of 1246 cm-1 (guaiacyl of lignin) was greater than that at wave number of 1328 cm-1 (deformation combination of syringyl and xylan) after fungal treatment. X-ray analysis showed the pretreated samples had a higher crystallinity than the untreated ones which might be due to the cleavage of amorphous fractions of cellulose. The pretreated samples have more fragile than the untreated ones confirmed by SEM. Crystalline allomorph calculated by XRD analysis showed that fungus pretreatment for 30 days has transformed triclinic structure of cellulose to monoclinic structure.
Co-Authors . Gunawan . Gunawan A. Aziz Darwis A. Herryandie Abdul Aziz Darwis Adhi Kusnadi Aida Farida Anas M. Fauzi Anggun Rahman Rahman Ani Suryani Ani Suryani Aninda Sekar ZAHRA Anwar Nur Anwar Nur ASEP RISWOKO ASTUTIATI NURHASANAH Bambang Prasetya Bambang Prasetya Bambang Prasetya Darwis, Abdul Aziz Dede Rais Diana Dewi Diana Dewi, Diana Diana Nurani Djumali Mangunwidjaja Djumali Mangunwidjaja Djumali Mangunwidjaja Dwi - Susilaningsih Dwi Purnomo Dwi Purnomo Dwi Setyaningsih DYAH NOOR HIDAYATI Dyah Noor Hidayati E. Gumbira-Sa’id E. Mardliyati Endang Gumbira Said Endang Warsiki Eni Dwi Islamiati Eni Dwi Islamiati Eni Dwi Islamiati, Eni Dwi Erwahyuni E Prabandani ERWAHYUNI ENDANG PRABANDARI ERWAHYUNI ENDANG PRABANDARI Erwahyuni Prabandari Erwahyuni Prabandari, Erwahyuni Essa Annisa Syadiah Etik Mardliyati Etik Mardliyati, Etik Etty Riani FENTRI PARAMITHA PUTRI Fitriani Kasim Gamma Irca Pradikta Gumbira-Sa'id, E Gustri Yeni Han Roliadi Hanifah FUADI Hanny Hafiar Hendri Muchtar Hendri Muchtar, Hendri Herastuti Sri I Nyoman Jaya Wistara Ike Sitoresmi M Purbowati Illah Sailah Irawadi Jamaran IS HELIANTI Isroi Isroi Isroi Joko Santoso Lanjar Sumarno Lanjar Sumarno Lestari, Puji Liesbetini Haditjaroko Liesbetini Hartoto Lucky Risanto M Irfani ABDULLAH M. ANWAR NUR M. Daud Mardliyati, Etik Muhammad Daud Muhammad Rasyid RAMADHAN Muhammad Tasrif Muhammad Tasrif Mulyorini Rahayuningsih Murdiyatmo, Untung N. A. Evalia Nabilah, Siti Nadia STEPANI Nastiti Siswi Indrasti nFN Misgiyarta NIKNIK NURHAYATI Ninik Purbosari Nur Atifah Nur Richana Nurika Asih SETIYANI Nusaibah - Nusaibah Prayoga SURYADARMA Rini Purnawati Rofiq Sunaryanto Rofiq Sunaryanto S Heris Anita S Joni Munarso Sa’id, E. Gumbira Sapta Raharja Prayoga Suryadarma dan T. Ikhsan Azmi Ani Suryani Sarifah Nurjanah Selvia Mahilda RIDHOHA Shinta PERMATASARI Siti Nabilah Sony Hartono Wijaya Sudirman Sudirman Suprihatin Suprihatin Surono SURONO Tri Panji Tun T. Irawadi Tun Tedja Irawadi TUN TEDJA IRAWADI Tun Tedja Irawadi Wahono Sumaryono Warsiki, Endang Wasrin Safii Wasrin Syafii dan Kurnia Sofyan Gustan Pari Buchari Widya Fatriasari Widya FATRIASARI Yandra Arkeman Yandra Arkenan Yeni, Gustri Yeni, Gustri