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HISTOPATHOLOGICAL CHANGES OF KIDNEY OF BROILER CHICKEN EXPOSED TO CHRONIC HEAT STRESS Irene Teh Kai Xin; Hani Plumeriastuti; Chairul Anwar; Kadek Rachmawati; Suzanita Utama; Djoko Legowo
Journal of Basic Medical Veterinary Vol. 8 No. 2 (2019): Journal of Basic Medical Veterinary, December 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i2.20411

Abstract

The aim of this research was to know histopathological changes of kidney of broiler chicken exposed to chronic heat stress. Twenty broilers were divided randomly into 2 groups, 10 broilers each are being exposed to chronic heat stress for 21 days. The first 21 days were the adaptation period in a chamber with temperature 24-28o C and humidity 40-55%. After 21 days, continue with exposure to heat stress in a chamber with temperature 36-40o C and humidity 50-65% in 8 hours per day. After exposed to chronic heat stress, kidney tissues were processed, and kidney tissue histopathological changes were evaluated by using the Klopfleisch modified scoring method. The data was analyzed by Mann – Whitney Test. The result of this research showed that chronic heat stress exposure causing the presence of degeneration of tubular epithelial cell, necrosis of tubular epithelial cell, necrosis of glomerular and interstitial infiltration. From the analysis data the overviewof multiparametric showed that when control group compared with the treatment group showed significantly difference (p<0.05). 
EFEK PEMBERIAN EKSTRAK RUMPUT KEBAR (Biophytum petersianum Klotzsch) TERHADAP JUMLAH SEL LEYDIG MENCIT (Mus musculus) JANTAN YANG DIPAPAR 2,3,7,8-TETRACHLORODIBENZO-P-DIOXIN Milla Nursadida; Hani Plumeriastuti; Yeni Dhamayanti; Widjiati Widjiati; Epy Muhammad Luqman; Arimbi Arimbi
Journal of Basic Medical Veterinary Vol. 8 No. 1 (2019): Journal of Basic Medical Veterinary, June 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i1.19998

Abstract

This study was aim to examine the effect of kebar grass extract (Biophytum petersianum Klotzsch) to against number leydig cells of mice (Mus musculus) by exposed 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD). Twenty five male mice (Mus musculus) 4 months with avarage body weight 20 g were used. These animals were divided into five groups (K(-), K(+), P1, P2 and P3). K(-) was treated with placebo, K(+) was treated by exposed TCCD single dose injection intaperitoneal 7µg/KgBw, P1 was treated by exposed TCCD single dose injection intaperitoneal 7µg/KgBw and kebar grass extract 0,045 mg/g Bw/day P2 was treated by exposed TCCD single dose injection intaperitoneal 7µg/KgBw and kebar grass extract 0,080 mg/g Bw/day, P3 was treated by exposed TCCD single dose injection intaperitoneal 7µg/KgBw and kebar grass extract 1,350 mg/g Bw/day. This research has been conducted for 53 days. The data were compared using ANOVA and Duncan test by SPSS 22.4 for windows. The result showed that Kebar Grass Extract in all of groups can prevent the damage of leydig cells in testis that exposed by TCCD significantly (p<0,05) and kebar grass extract 0,135 mg/kgBw/day can increase amount of leydig cells maximaly.
POTENSI KANDUNGAN ANTIOKSIDAN RUMPUT KEBAR (Biophytum Petersianum Klotzsch) TERHADAP APOPTOSIS DAN GAMBARAN HISTOPATOLOGIS TESTIS PADA MENCIT (MUS MUSCULUS) YANG DIPAPAR 2,3,7,8-Tetrachlorodibenzo-p-dioxin Dewita Dewita; Widjiati Widjiati; Rimayanti Rimayanti; Tri Wahyu Suprayogi; Mas'ud Hariadi; Hani Plumeriastuti
Journal of Basic Medical Veterinary Vol. 8 No. 1 (2019): Journal of Basic Medical Veterinary, June 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i1.19986

Abstract

2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is the most toxic compound in the dioxin group. This compound is a pollutant for the environment and very harmful to human health and enter the body through the mucous membranes in the mouth and the respiratory tract and can be transmitted through the placenta and lactation. The aim of this study was to know the antioxidantpotency of to resolve reproduction disturbance caused by TCDD exposure.Thirty Balb/C male mice were divided into five different groups, the negative control group, a positive control group exposed to TCDD at a dose of 7 μg/kg BW, P1 group of groups exposed to TCDD doses of 7 μg / kg BW and given Biophytum petersianum extract 0.05mg/gBB/day, group P2 group exposed to TCDD doses of 7μg/kgBW and given 0.080mg/gBB/day, and group P3 were exposed to TCDD dose of 7μg/kgBB and given the extract of Biophytum petersianum 0.135mg/gBB/day during day 2 to day 55. On the 56th day the mice were sacrificed and apoptotic examination and spermatogenic cell histopathological features were performed on the testis. The results showed that: P2 (0,433 ± 0,497; p<0,05) and P3 (0,200 ± 0,000 p<0,05) groups were the most effective group in decreasing spermatogenic cell apoptosis compared to positive control group (2,933 ± 1,5832 p<0,05). The Johnsen score result showed that P2 (9,400 ± 0,420) and P3 (9,800 ± 0,253) groups improving the histopathologic picture of spermatogenic cells in seminiferous tubules compared to positive control group (7,20 ± 0,400) p<0,05. Conclusion of this study were Biophytum petersianum is effective to solve reproduction disturbances caused by exposure of TCDD and the P3 group is the most effective group.
HISTOPATHOLOGICAL APPEARANCE OF THYMUS ON BROILER UNDER CHRONIC HEAT STRESS Ratna Andriani; Arimbi Arimbi; Dadik Rahardjo; Hani Plumeriastuti; Djoko Legowo; Eka Pramyrtha Hestianah
Journal of Basic Medical Veterinary Vol. 8 No. 1 (2019): Journal of Basic Medical Veterinary, June 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i1.19987

Abstract

The aim of this research was to know histopathological change of broiler’s thymus which exposed by chronic heat stress. The results revealed that there were negative impact on immune organ (thymus). Broiler that exposed to chronic heat stress showed significantly increased number of heterophil and apoptosis histiocytes and cell depletion. There were 20 broilers that divided into 2 groups, each group consist of 10 broiler. Control group (P0) reared at temperature 24-28oC and humidity 40-55% and Heat Stress group (P1) reared at temperature 36-40oC on the litter housing system at Experimental Animals Laboratory, Faculty of Veterinary Medicine, Universitas Airlangga. Before the treatments, broiler had been reared from day-old-chicks to 21-day-old, then continued with heat stress condition from day 22 to 42. Thymus was collected on day 42 to examine histopathological change of thymus and analyzed using Mann-Whitney Test. The results showed significant difference (P<0.05) between Control group and HS (Heat Stress) group by increased number of heterophil, apoptosis histiocytes and cell depletion.
PENGARUH PEMBERIAN PROPOLIS LEBAH TERHADAP GAMBARAN HISTOPATOLOGI HEPAR MENCIT (Mus musculus) BETINA YANG DIPAPAR LOGAM BERAT PB ASETAT [Pb(C2H3O2)2] Sartika Dewi Rachmani; Erma Safitri; Roesno Darsono; Eka Pramyrtha Hestianah; Hani Plumeriastuti; Kuncoro Puguh Santoso
Journal of Basic Medical Veterinary Vol. 8 No. 1 (2019): Journal of Basic Medical Veterinary, June 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i1.19988

Abstract

Lead acetate in the hepatobiliary system may cause peroxidation catalysis of unsaturated fatty acids, reduce nitrogenoxide and increase hydroxyl radical. Lead acetate produces oxidative stress characterized by free radical formation and inhibits lipid peroxidation. Giving antioxidants can neutralize free radicals from the detrimental effects that arise on the process or excess oxidation reactions. The purpose of this research was to find out how the effect of bee propolis on histopathologic images of hepatic mice (Mus musculus) of females exposed to lead acetate [Pb(C2H3O2)2]. The subjects were 25 mice (Mus musculus) mushulus of BALB/C strain with average weight 25-30 gram and 8 weeks old, divided into 5 treatment groups, each consisting of 5 heads per group. The K- group was given a Tween 80 solution at a doses of 0.5 mg/kgBW for 20 consecutive days. The K+ group, which was given only lead acetate at a doses of 10 mg/kgBW orally for 10 days. P1, P2, and P3 were given 10 mg/kgBW lead acetate solution orally for 10 days. The following 10 days were given bee propolis with doses of P1 200 mg/kgBW, P2 400 mg/kgBW, and P3 800 mg/kgBW. On the 21th days the mice were dissected, to observe the extent of the damage. All data were performed using a statistical test with Kruskal Wallis test and if there was a marked difference between treatment groups (p<0.05), then the Mann-Whitney test was followed. The results obtained that bee propolis can repair hepatic cell damage in mice (Mus musculus) of females exposed to lead acetate. Increased dose of bee propolis is ineffective in repairing hepatic cell damage in mice (Mus musculus) of females exposed to lead acetate.
THE EFFECT OF POLYPROPYLENE PLASTIC RESIDUE ON HEATED PALM OIL AGAINST HISTOPATHOLOGICAL CHANGES OF SMALL INTESTINE ON MALE WHITE RAT (Rattus norvegicus) WISTAR STRAIN Aulia Puspa Amaris; Lita Rakhma Yustinasari; Djoko Legowo; Hani Plumeriastuti; Chairul Anwar; Nove Hidajati
Journal of Basic Medical Veterinary Vol. 8 No. 2 (2019): Journal of Basic Medical Veterinary, December 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i2.20409

Abstract

This study was conducted to investigate the effect of polypropylene residue on heated palm oil against histopathological changes of small intestine on male white rat (Rattus norvegicus) wistar strain. Eighteen male white rats divided into three groups and were administered via peroral gavage with different treatments for five weeks. The treatment were Control (84 grams palm oil), P1 (84 grams palm oil with 2.3 grams plastic), and P2 (84 grams palm oil with 6.81 grams plastic). The histopathological changes of small intestine were examined by using Barthel Scoring method was analysed using Kruskal Wallis and continued with Mann-Whitney test. The result showed that were significant difference (p<0.05) between treatment groups. The worse damage of polypropylene plastic residue was 6.81 grams.
PENGUJIAN VAKSIN HEPATITIS B FASE SUBKRONIS TERHADAP BERAT ORGAN DAN DIAMETER PULPA PUTIH LIMPA TIKUS PUTIH (Rattus norvegicus) Afifatus Anindya; Kuncoro Puguh Santoso; Fedik Abdul Rantam; Kadek Rachmawati; Chairul Anwar Nidom; Thomas Valentinus Widiyatno; Hani Plumeriastuti
Journal of Basic Medical Veterinary Vol. 8 No. 2 (2019): Journal of Basic Medical Veterinary, December 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i2.20410

Abstract

This study aims to determine the effect of hepatitis B vaccine of spleen weight and white pulp diameter in rat (Rattus norvegicus). This study used 20 male wistar rats aged 8 weeks with an average weight of 200-250 grams, which was divided into four groups. Control group (P0) was given PBS solution, treatment group 1 (P1) was given type 1 hepatitis B vaccine, treatment group 2 was given type 2 hepatitis B vaccine, and treatment group 3 (P3) was given type 3 hepatitis B vaccine. Dose administered 0.4 ml and given repeatedly at days 0,30, and 60. Animals model to euthanize on day 75 for sampling. The spleen organ is weighed after the necropsing process and then the spleen is stored for the preparation of histopathologic with HE staining (Hematoxylin Eosin). The data obtained are weight data of spleen organ and spleen white pulp diameter. The results showed the highest weight of spleen organ 0.92 gram in group P0 and the largest white pulp diameter 34,34 μm in group P1. The results of data analyzed using one-way ANOVA (Analysis of Variance) and continued with Duncan alpha test. One Way Anova and Duncan alpha test showed that there were non significantly different between groups (p>0.05). The conclusion is we know that hepatitis B vaccine type 1,2, and 3 can’t give the effect for spleen weight and white pulp diameter in rat (Rattus norvegicus).
THE EFFECT OF POLYPROPYLENE PLASTIC RESIDUE ON HEATED PALM OIL AGAINST THE HISTOPATHOLOGICAL CHANGES OF KIDNEY ON MALE RATS (Rattus norvegicus) Shahani Azpriyanne Cahyono; Hana Eliyani; Thomas Valentinus Widiyatno; Hani Plumeriastuti; Nove Hidajati; Kuncoro Puguh Santoso
Journal of Basic Medical Veterinary Vol. 8 No. 2 (2019): Journal of Basic Medical Veterinary, December 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i2.20415

Abstract

This research was conducted to observe the effect of polypropylene plastic residue on heated palm oil against the histopathological changes on rat’s kidney. There were 18 male rats (Rattus norvegicus) that used as the examination animal and were divided into three groups and were given the mixture of heated palm oil and polypropylene plastic per oral with intubation needle. The treatment was done for five weeks. The treatment for P0 group was 84 g of palm oil, for P1 was 84 g of palm oil with 2.3 g of plastic, and for the P2 was 84 g of palm oil with 6.8 g of plastic. The histopathological changes of the kidney were observed using microscope with 400x magnification and the data was analysed using Kruskal-Wallis and continued with Mann Whitney U test. The result showed that there were significant differences between three groups of treatment (P<0.05). There were effects of polypropylene plastic residue on heated palm oil against the histopathological changes of kidney in this research such as cast, epithelial sloughing and necrosis in the mild scale.
EFEKTIVITAS PEMBERIAN NANOPARTIKEL EKSTRAK DAUN JUWET (Syzygium cumini) SEBAGAI ADJUVANT TERAPI TERHADAP GAMBARANHISTOPATOLOGI PULMO MENCIT (Mus musculus) YANG DIINFEKSI Plasmodium bhergei Zerlinda Dyah Ayu; Lilik Maslachah; Koesnoto Supranianondo; Endang Suprihati; Hani Plumeriastuti; Rahmi Sugihartuti
Journal of Basic Medical Veterinary Vol. 9 No. 1 (2020): Journal of Basic Medical Veterinary, June 2020
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v9i1.21091

Abstract

The purpose of this study was to learn how to use juwet nanoparticle leaf extract (Syzygium cumini) as an adjunct therapy in pulmonary histopathological mice (Mus musculus) supported by Plasmodium berghei. This study used 30 rats divided into six groups consisting of K0 (disagree without agreement), P1 (fight without agreement), P2 (fight and dispute with chloroquine), P3 (fight and talk with juwet leaf extract), P4 (useful and useful with juwet leaf extract)) and P5 (extracts and combinations given with chloropine and juwet leaf extract nanoparticles) protected by 1x105 in 0.2 ml of Plasmodium berghei. Data were analyzed by Kruskal Wallis using SPSS and continued with the Mann-Whitney test. The results of this study showed that there were significant differences (p <0.05) between P2 and P5, but did not show a significant difference (p> 0.05) with P1, P3 and P4. Based on this study, can be witnessed by juwet nanoparticles (Syzygium Cumini) additional therapeutic leaf extract which is expected to reduce pulmonary damage (hemmoragi, alveolar congestion, edema, hemosiderin) mice (Mus musculus) infected with Plasmodium berghei. 
PENGARUH PEMAPARAN KARBOFURAN PADA INDUK MENCIT (Mus musculus) TERHADAP GAMBARAN HISTOPATOLOGI GINJAL ANAK MENCIT MASA LAKTASI Edi Purnomo; Epy Muhammad Luqman; Hermin Ratnani; Hani Plumeriastuti; Maslichah Mafruchati; Yeni Dhamayanti
Journal of Basic Medical Veterinary Vol. 9 No. 1 (2020): Journal of Basic Medical Veterinary, June 2020
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v9i1.21095

Abstract

Carbofuran is a broad spectrum carbamat insecticide which is used in various farm practices. An exposure of carbofuran has been reported able to induce Reactive Oxygen Species (ROS) or free radical in kidney. Free radical caused cells damage. The present study was designed to investigate the effect of carbofuran in kidneys histopathologic of mice pup whose dam were exposed by carbofuran during the lactation period. High levels of ROS can bind to various biomolecules such as proteins, lipids and nucleic acids so that carbofuran has a negative effect on tissue functions and structures. Free radical caused changes in the pathological conditions of the tissue including the kidneys. The dams were devided into four groups of five animals each. P0 animal group was received aquadest 0,1 ml per body weight (BW), P1 animal group was treated carbofuran at the dose of 1/16 LD50 (0,3125 mg per BW), P2 animal group was treated carbofuran at the dose of 1/8 LD50 (0, 625 mg per BW), and P3 animal group was treated carbofuran at the dose of 1/4 LD50 (1,25 mg per BW). The carbofuran was exposed via oral postnatal day 1st until 9th postnatal day after delivery. At postnatal day 10th, offspring were sacrified. The kidney of mice pup was collected and processed for microscopic examination with hematoxilin-eosin staining. Microscopic observations on the histology of the mice pup kidney showed an increase in the degree of kidney damage as an increase in the dose of carbofuran given to the dams. The results showed tubular changes in the form of tubular degeneration, inflammatory cell infiltration and tubular necrosis in the kidney of mice pups.