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PENGUJIAN VAKSIN HEPATITIS B FASE SUBKRONIS TERHADAP BERAT ORGAN DAN DIAMETER PULPA PUTIH LIMPA TIKUS PUTIH (Rattus norvegicus) Afifatus Anindya; Kuncoro Puguh Santoso; Fedik Abdul Rantam; Kadek Rachmawati; Chairul Anwar Nidom; Thomas Valentinus Widiyatno; Hani Plumeriastuti
Journal of Basic Medical Veterinary Vol. 8 No. 2 (2019): Journal of Basic Medical Veterinary, December 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i2.20410

Abstract

This study aims to determine the effect of hepatitis B vaccine of spleen weight and white pulp diameter in rat (Rattus norvegicus). This study used 20 male wistar rats aged 8 weeks with an average weight of 200-250 grams, which was divided into four groups. Control group (P0) was given PBS solution, treatment group 1 (P1) was given type 1 hepatitis B vaccine, treatment group 2 was given type 2 hepatitis B vaccine, and treatment group 3 (P3) was given type 3 hepatitis B vaccine. Dose administered 0.4 ml and given repeatedly at days 0,30, and 60. Animals model to euthanize on day 75 for sampling. The spleen organ is weighed after the necropsing process and then the spleen is stored for the preparation of histopathologic with HE staining (Hematoxylin Eosin). The data obtained are weight data of spleen organ and spleen white pulp diameter. The results showed the highest weight of spleen organ 0.92 gram in group P0 and the largest white pulp diameter 34,34 μm in group P1. The results of data analyzed using one-way ANOVA (Analysis of Variance) and continued with Duncan alpha test. One Way Anova and Duncan alpha test showed that there were non significantly different between groups (p>0.05). The conclusion is we know that hepatitis B vaccine type 1,2, and 3 can’t give the effect for spleen weight and white pulp diameter in rat (Rattus norvegicus).
PENGARUH PEMBERIAN PROPOLIS LEBAH TERHADAP GAMBARAN HISTOPATOLOGI HEPAR MENCIT (Mus musculus) BETINA YANG DIPAPAR LOGAM BERAT PB ASETAT [Pb(C2H3O2)2] Sartika Dewi Rachmani; Erma Safitri; Roesno Darsono; Eka Pramyrtha Hestianah; Hani Plumeriastuti; Kuncoro Puguh Santoso
Journal of Basic Medical Veterinary Vol. 8 No. 1 (2019): Journal of Basic Medical Veterinary, June 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i1.19988

Abstract

Lead acetate in the hepatobiliary system may cause peroxidation catalysis of unsaturated fatty acids, reduce nitrogenoxide and increase hydroxyl radical. Lead acetate produces oxidative stress characterized by free radical formation and inhibits lipid peroxidation. Giving antioxidants can neutralize free radicals from the detrimental effects that arise on the process or excess oxidation reactions. The purpose of this research was to find out how the effect of bee propolis on histopathologic images of hepatic mice (Mus musculus) of females exposed to lead acetate [Pb(C2H3O2)2]. The subjects were 25 mice (Mus musculus) mushulus of BALB/C strain with average weight 25-30 gram and 8 weeks old, divided into 5 treatment groups, each consisting of 5 heads per group. The K- group was given a Tween 80 solution at a doses of 0.5 mg/kgBW for 20 consecutive days. The K+ group, which was given only lead acetate at a doses of 10 mg/kgBW orally for 10 days. P1, P2, and P3 were given 10 mg/kgBW lead acetate solution orally for 10 days. The following 10 days were given bee propolis with doses of P1 200 mg/kgBW, P2 400 mg/kgBW, and P3 800 mg/kgBW. On the 21th days the mice were dissected, to observe the extent of the damage. All data were performed using a statistical test with Kruskal Wallis test and if there was a marked difference between treatment groups (p<0.05), then the Mann-Whitney test was followed. The results obtained that bee propolis can repair hepatic cell damage in mice (Mus musculus) of females exposed to lead acetate. Increased dose of bee propolis is ineffective in repairing hepatic cell damage in mice (Mus musculus) of females exposed to lead acetate.
PENGARUH PEMBERIAN EKSTRAK SAMBILOTO (Andrographis Paniculata) TERHADAP GAMBARAN HISTOPATOLOGI GINJAL YANG DIINDUKSI GENTAMISIN PADA TIKUS (Rattus norvegicus) Anggi Dwi Nugrahani; Lianny Nangoi; Dewa Ketut Meles; Thomas Valentinus Widiyatno; Iwan Sahrial Hamid; Kuncoro Puguh Santoso
Journal of Basic Medical Veterinary Vol. 8 No. 1 (2019): Journal of Basic Medical Veterinary, June 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i1.19989

Abstract

This research was aimed to know the effect of leaves extract of sambiloto as a nephroprotector on renal histopathology features induced by gentamicin in white rats (Rattus norvegicus). The effect of leaves extract of sambiloto was tested in 25 rats. Twenty five male white rat were randomly divided into five groups; K- as negative control was given carboxy methyl cellulosa in 15 days, P0 was given carboxyl methyl cellulosa in 15 days, P1 was given leaves extract of sambiloto of 177 mg/kg bw/day/po in 15 days, P2 was given leaves extract of sambiloto of 189 mg/kg bw/day/po in 15 days, P3 was given leaves extract of sambiloto of 239,5 mg/kg bw/day/po in 15 days. On the 15 day of experimental, P0, P1, P2, P3 was given 200 mg/kg bw of gentamicin. Gentamicin solutions on P0, P1, P2 and P3 treated through intramuscular injection. After 24 hours of gentamicin induced, take the rats renal by laparotomy and then making preparations histopathology with staining Haematoxylin-Eosin (HE). Based on the result of Kruskall-Wallis statistical analysis on renal histopathology showed this research that the treatment have significant difference (p < 0,05). The leaves extract of sambiloto treated group (P3) gave the best value on the renal morfology, it gave a good effects in preventing nephrotoxicity in white rats.
EFFECT OF Polygonum minus (KESUM) LEAVES ETHANOLIC EXTRACT ON HISTOPATHOLOGICAL CHANGES ON THE WALL AORTA OF MICE (Mus musculus) INDUCED BY CADMIUM CHLORIDE ANTIOXIDANT Putri Anggraheni Kusumaningrum; Lita Rakhma Yustinasari; Iwan Sahrial Hamid; Sri Agus Sudjarwo; Kuncoro Puguh Santoso; Chairul Anwar
Journal of Basic Medical Veterinary Vol. 8 No. 2 (2019): Journal of Basic Medical Veterinary, December 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i2.20408

Abstract

This study was conducted to investigate the protective effect Polygonum minus leaves extract on the histopathological changes on aorta wall of mice (Mus musculus) induced by cadmium chloride. Thirty male mice were divided into five groups and were administered via intragastric gavage with different treatments for 21 days. The treatment were Negative Control (CMC Na 0.5% solution + aquadest), Positive Control (CMC Na0.5% solution + 8 mg/kg bw of mercuric chloride), Treatment 1, 2, and 3 (200, 400, and 800 mg/kg bw of Polygonum minus leaves extract respectively + 8 mg/kg bw of cadmium chloride). The microscopec examine followed analyzed by One Way ANOVA continued with Duncan test for counting the amount of foam cell and the histopathological changes of wall aorta were examined by using Ifora Scoring method was analysed using Kruskal Wallis and continued with Mann-Whitney test. The result showed Polygonum minus leaves extract could protect wall aorta of mice from the damage effect of cadmium chloride. The best dose of Polygonum minus on this research was 400 mg/kg bw.
THE EFFECT OF POLYPROPYLENE PLASTIC RESIDUE ON HEATED PALM OIL AGAINST THE HISTOPATHOLOGICAL CHANGES OF KIDNEY ON MALE RATS (Rattus norvegicus) Shahani Azpriyanne Cahyono; Hana Eliyani; Thomas Valentinus Widiyatno; Hani Plumeriastuti; Nove Hidajati; Kuncoro Puguh Santoso
Journal of Basic Medical Veterinary Vol. 8 No. 2 (2019): Journal of Basic Medical Veterinary, December 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i2.20415

Abstract

This research was conducted to observe the effect of polypropylene plastic residue on heated palm oil against the histopathological changes on rat’s kidney. There were 18 male rats (Rattus norvegicus) that used as the examination animal and were divided into three groups and were given the mixture of heated palm oil and polypropylene plastic per oral with intubation needle. The treatment was done for five weeks. The treatment for P0 group was 84 g of palm oil, for P1 was 84 g of palm oil with 2.3 g of plastic, and for the P2 was 84 g of palm oil with 6.8 g of plastic. The histopathological changes of the kidney were observed using microscope with 400x magnification and the data was analysed using Kruskal-Wallis and continued with Mann Whitney U test. The result showed that there were significant differences between three groups of treatment (P<0.05). There were effects of polypropylene plastic residue on heated palm oil against the histopathological changes of kidney in this research such as cast, epithelial sloughing and necrosis in the mild scale.