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All Journal Jurnal Veteriner Jurnal Medik Veteriner Journal of Parasite Science Journal of Applied Veterinary Science and Technology journal of Basic Medical Veterinary

Kuncoro Puguh Santoso

Department Of Basic Veterinary Medicine - Faculty Of Veterinary Medicine, Universitas Airlangga

Author-ID : 4007359

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

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Articles

Aktivitas Superoxide Dismutase pada Serum Darah Babi Landrace yang Disembelih dengan Metode Electrical Stunning Shabrina Fauzia Prayoga; Kuncoro Puguh Santoso; Nove Hidajati; Faisal Fikri; Maya Nurwartanti Yunita; Muhammad Thohawi Elziyad Purnama
Jurnal Veteriner Vol 22 No 2 (2021)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Tujuan penelitian ini untuk menentukan aktivitas Superoxide Dismutase (SOD) pada babi Landrace yang disembelih dengan metode stunning dan non-stunning. Sebanyak 18 ekor babi Landrace jantan dewasa yang telah dikebiri dengan masing-masing bobot 115±20 kg dibagi menjadi dua kelompok, yakni stunning dan non-stunning dengan pengujian serum pada pre dan post treatment. Electrical stunning dilakukan dengan aliran listrik pada dua elektroda sebesar 220 volt dan 1,3 ampere. Sampel darah diambil sebanyak 3 mL, kemudian diuji aktivitas SODnya.. Hasil aktivitas SOD dianalisis dengan uji T berpasangan untuk membandingkan aktivitas SOD serum antara stunning dan non-stunning pada fase pre dan post treatment. Hasil stunning menunjukkan penurunan signifikan (p<0,05) aktivitas SOD sebesar 56,12±54,47 dibandingkan non-stunning sebesar 137,84±66,63. Aktivitas SOD yang rendah tampak signifikan (p<0,05) juga pada pre-treatment dibandingkan dengan post-treatment. Dapat disimpulkan bahwa penyembelihan dengan metode electrical stunning menurunkan aktivitas SOD pada serum babi Landrace.

Prevalence of Nematodiasis in Omasum and Abomasum of Goats Slaughtered at Pegirian Surabaya Slaughter House Through Gatrointestinal Surgery Method Dewi Mariyam; Sri Mumpuni Sosiawati; Kuncoro Puguh Santoso; Setiawan Koesdarto; Sarmanu Sarmanu; Ira Sari Yudaniayanti
Journal of Parasite Science (JoPS) Vol. 2 No. 1 (2018): Journal of Parasite Science
Publisher : Universitas Airlangga

The purpose of this research is to determine the kind of nematodes and to determine the prevalence result. Thirtysix samples of omasum and abomasum were collected from RPH Pegirian from September-November 2017 then using gastrointestinal surgery to get fresh adult worm, afterwards using Semichen-Acetic Carmine stainning method to makes the object easy to microscopic identify. The result showed that species was found Haemonchus contortus, as an identify result show that adult worm has a character on a anterior part called cervical papilae, female worm has a barber’s pole on body also posterior part has a vulva flap, posterior part of male worm has a bursa copulatrix, spicula, gubernaculum and dorsal rays looks like Y shape. Seventeen positive samples and the prevalence rate was 47,2%, 273 adult worms and female worms 215 (78,75%) and the male worms 58 (21,25%). Chi-Square statistical test (P <0.05) showed that 13 positive samples of kambing kacang (65%) were higher infected Haemonchosis infection than 4 potitive samples of PE (25%).

Microscophy Identification of Toxocara cati First Stage Larvae and Second Stage Larvae Eny Coolfina Simarmata; Kusnoto Kusnoto; Mochamad Lazuardi; Setiawan Koesdarto; Endang Suprihati; Kuncoro Puguh Santoso
Journal of Parasite Science (JoPS) Vol. 3 No. 1 (2019): Journal of Parasite Science
Publisher : Universitas Airlangga

This study was aimed to identify the ultra structural morphology of Toxocara cati First Stage Larvae and Second Stage Larvae using Light Microscope. Toxocara cati larvae were obtained from adult worm eggs then were treated in phospat buffer saline with comparasion 1 : 1 until it reached the stage of larvae 1 and 2. The shell of egg Toxocara cati were thick and usually consist of three layers. The first layer was inner membrane, the second layer was middle layer and the third was outer layer. The results of larvae morphology that were identified using light microscope showed that the anterior part of Toxocara cati first stage larvae has a dorsal lip and esophagus and intestine on the posterior part but it could not be identified perfectly. The anterior part of Toxocara cati second stage larvae that were identified has three lips that leads directly into the oesophagus. Three lips on the anterior part of Toxocara cati larvae 2 consist of a dorsal lip and two subventral lips. Morphological of Toxocara cati larvae similar with the Toxocara cati adult worms because morphology of larvae and adult stage was difficult to differentiated.

Necrosis Description of Mice Liver Induced with Monosodium Glutamate and Methanol Robusta Coffee Bean Extract (Coffea canephora) Tisya Yumn Al-Zuhroh; Kuncoro Puguh Santoso; Maya Nurwartanti Yunita; Nove Hidajati; Ratih Novita Praja
Jurnal Medik Veteriner Vol. 4 No. 2 (2021): October
Publisher : Faculty of Veterinary Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol4.iss2.2021.213-220

This study aimed to analyze the effect of methanol robusta coffee bean extract (Coffea canephora) on liver histopathological description in mice induced with monosodium glutamate (MSG). This study was used 20 male mice for 5 treatment groups: K + (MSG 0.12 mg and Vitamin C 6 mg), K- (MSG 0.12 mg and CMC Na 0.1 ml), P1 (MSG 0.12 mg and RCE 0.1 mg), P2 (MSG 0.12 mg and RCE 0.2 mg), and P3 (MSG 0.12 mg and RCE 0.4 mg) per orally for 42 days. Liver histological scores were analyzed using Kruskal Wallis test. The results of Kruskal Wallis test scored p=0,391 which indicates that the data is not significant (p>0.05). In the control and treatment groups there were no significant. In conclusion, the giving methanol robusta coffee bean extract did not show a decrease in hepatosite damage that was different from the negative control treatment group.

Detection of Salmonella sp. on Layer Chicken Eggs Sold at The Vegetable Market of Magetan Regency Brillian Inawulan Syamsiar; Kuncoro Puguh Santoso; Ira Sari Yudaniayati; Diyantoro Diyantoro
Journal of Applied Veterinary Science And Technology Vol. 1 No. 2 (2020): October 2020
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/javest.V1.I2.2020.34-38

Background: Eggs are poultry products that cannot be separated from the possibility of Salmonella contamination. Traditional markets are places where consumers can get chicken eggs with poor hygiene and sanitation aspects. Habits of today's society that often consumes raw or undercooked eggs can trigger Salmonellosis. Purpose: To identify the presence of Salmonella sp. in eggs sold in Magetan Regency Vegetable Market. Method: Sampling of eggs from different random stores. The eggs then proceeded with selection pre-enrichment, selective enrichment, isolation, and identification. Results: The handling of eggs in the Magetan Regency Market was quite good in its storage period, and purebred eggs sold in the Magetan Regency Market were negatively contaminated with Salmonella sp. Conclusion: Broiler chicken eggs sold in Magetan Vegetable Market are safe to consume.

PENGARUH PEMBERIAN PROPOLIS LEBAH TERHADAP GAMBARAN HISTOPATOLOGI HEPAR MENCIT (Mus musculus) BETINA YANG DIPAPAR LOGAM BERAT PB ASETAT [Pb(C2H3O2)2] Sartika Dewi Rachmani; Erma Safitri; Roesno Darsono; Eka Pramyrtha Hestianah; Hani Plumeriastuti; Kuncoro Puguh Santoso
Journal of Basic Medical Veterinary Vol. 8 No. 1 (2019): Journal of Basic Medical Veterinary, June 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i1.19988

Lead acetate in the hepatobiliary system may cause peroxidation catalysis of unsaturated fatty acids, reduce nitrogenoxide and increase hydroxyl radical. Lead acetate produces oxidative stress characterized by free radical formation and inhibits lipid peroxidation. Giving antioxidants can neutralize free radicals from the detrimental effects that arise on the process or excess oxidation reactions. The purpose of this research was to find out how the effect of bee propolis on histopathologic images of hepatic mice (Mus musculus) of females exposed to lead acetate [Pb(C2H3O2)2]. The subjects were 25 mice (Mus musculus) mushulus of BALB/C strain with average weight 25-30 gram and 8 weeks old, divided into 5 treatment groups, each consisting of 5 heads per group. The K- group was given a Tween 80 solution at a doses of 0.5 mg/kgBW for 20 consecutive days. The K+ group, which was given only lead acetate at a doses of 10 mg/kgBW orally for 10 days. P1, P2, and P3 were given 10 mg/kgBW lead acetate solution orally for 10 days. The following 10 days were given bee propolis with doses of P1 200 mg/kgBW, P2 400 mg/kgBW, and P3 800 mg/kgBW. On the 21th days the mice were dissected, to observe the extent of the damage. All data were performed using a statistical test with Kruskal Wallis test and if there was a marked difference between treatment groups (p<0.05), then the Mann-Whitney test was followed. The results obtained that bee propolis can repair hepatic cell damage in mice (Mus musculus) of females exposed to lead acetate. Increased dose of bee propolis is ineffective in repairing hepatic cell damage in mice (Mus musculus) of females exposed to lead acetate.

PENGARUH PEMBERIAN EKSTRAK SAMBILOTO (Andrographis Paniculata) TERHADAP GAMBARAN HISTOPATOLOGI GINJAL YANG DIINDUKSI GENTAMISIN PADA TIKUS (Rattus norvegicus) Anggi Dwi Nugrahani; Lianny Nangoi; Dewa Ketut Meles; Thomas Valentinus Widiyatno; Iwan Sahrial Hamid; Kuncoro Puguh Santoso
Journal of Basic Medical Veterinary Vol. 8 No. 1 (2019): Journal of Basic Medical Veterinary, June 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i1.19989

This research was aimed to know the effect of leaves extract of sambiloto as a nephroprotector on renal histopathology features induced by gentamicin in white rats (Rattus norvegicus). The effect of leaves extract of sambiloto was tested in 25 rats. Twenty five male white rat were randomly divided into five groups; K- as negative control was given carboxy methyl cellulosa in 15 days, P0 was given carboxyl methyl cellulosa in 15 days, P1 was given leaves extract of sambiloto of 177 mg/kg bw/day/po in 15 days, P2 was given leaves extract of sambiloto of 189 mg/kg bw/day/po in 15 days, P3 was given leaves extract of sambiloto of 239,5 mg/kg bw/day/po in 15 days. On the 15 day of experimental, P0, P1, P2, P3 was given 200 mg/kg bw of gentamicin. Gentamicin solutions on P0, P1, P2 and P3 treated through intramuscular injection. After 24 hours of gentamicin induced, take the rats renal by laparotomy and then making preparations histopathology with staining Haematoxylin-Eosin (HE). Based on the result of Kruskall-Wallis statistical analysis on renal histopathology showed this research that the treatment have significant difference (p < 0,05). The leaves extract of sambiloto treated group (P3) gave the best value on the renal morfology, it gave a good effects in preventing nephrotoxicity in white rats.

EFFECT OF Polygonum minus (KESUM) LEAVES ETHANOLIC EXTRACT ON HISTOPATHOLOGICAL CHANGES ON THE WALL AORTA OF MICE (Mus musculus) INDUCED BY CADMIUM CHLORIDE ANTIOXIDANT Putri Anggraheni Kusumaningrum; Lita Rakhma Yustinasari; Iwan Sahrial Hamid; Sri Agus Sudjarwo; Kuncoro Puguh Santoso; Chairul Anwar
Journal of Basic Medical Veterinary Vol. 8 No. 2 (2019): Journal of Basic Medical Veterinary, December 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i2.20408

This study was conducted to investigate the protective effect Polygonum minus leaves extract on the histopathological changes on aorta wall of mice (Mus musculus) induced by cadmium chloride. Thirty male mice were divided into five groups and were administered via intragastric gavage with different treatments for 21 days. The treatment were Negative Control (CMC Na 0.5% solution + aquadest), Positive Control (CMC Na0.5% solution + 8 mg/kg bw of mercuric chloride), Treatment 1, 2, and 3 (200, 400, and 800 mg/kg bw of Polygonum minus leaves extract respectively + 8 mg/kg bw of cadmium chloride). The microscopec examine followed analyzed by One Way ANOVA continued with Duncan test for counting the amount of foam cell and the histopathological changes of wall aorta were examined by using Ifora Scoring method was analysed using Kruskal Wallis and continued with Mann-Whitney test. The result showed Polygonum minus leaves extract could protect wall aorta of mice from the damage effect of cadmium chloride. The best dose of Polygonum minus on this research was 400 mg/kg bw.

PENGUJIAN VAKSIN HEPATITIS B FASE SUBKRONIS TERHADAP BERAT ORGAN DAN DIAMETER PULPA PUTIH LIMPA TIKUS PUTIH (Rattus norvegicus) Afifatus Anindya; Kuncoro Puguh Santoso; Fedik Abdul Rantam; Kadek Rachmawati; Chairul Anwar Nidom; Thomas Valentinus Widiyatno; Hani Plumeriastuti
Journal of Basic Medical Veterinary Vol. 8 No. 2 (2019): Journal of Basic Medical Veterinary, December 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i2.20410

This study aims to determine the effect of hepatitis B vaccine of spleen weight and white pulp diameter in rat (Rattus norvegicus). This study used 20 male wistar rats aged 8 weeks with an average weight of 200-250 grams, which was divided into four groups. Control group (P0) was given PBS solution, treatment group 1 (P1) was given type 1 hepatitis B vaccine, treatment group 2 was given type 2 hepatitis B vaccine, and treatment group 3 (P3) was given type 3 hepatitis B vaccine. Dose administered 0.4 ml and given repeatedly at days 0,30, and 60. Animals model to euthanize on day 75 for sampling. The spleen organ is weighed after the necropsing process and then the spleen is stored for the preparation of histopathologic with HE staining (Hematoxylin Eosin). The data obtained are weight data of spleen organ and spleen white pulp diameter. The results showed the highest weight of spleen organ 0.92 gram in group P0 and the largest white pulp diameter 34,34 μm in group P1. The results of data analyzed using one-way ANOVA (Analysis of Variance) and continued with Duncan alpha test. One Way Anova and Duncan alpha test showed that there were non significantly different between groups (p>0.05). The conclusion is we know that hepatitis B vaccine type 1,2, and 3 can’t give the effect for spleen weight and white pulp diameter in rat (Rattus norvegicus).

THE EFFECT OF POLYPROPYLENE PLASTIC RESIDUE ON HEATED PALM OIL AGAINST THE HISTOPATHOLOGICAL CHANGES OF KIDNEY ON MALE RATS (Rattus norvegicus) Shahani Azpriyanne Cahyono; Hana Eliyani; Thomas Valentinus Widiyatno; Hani Plumeriastuti; Nove Hidajati; Kuncoro Puguh Santoso
Journal of Basic Medical Veterinary Vol. 8 No. 2 (2019): Journal of Basic Medical Veterinary, December 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i2.20415

This research was conducted to observe the effect of polypropylene plastic residue on heated palm oil against the histopathological changes on rat’s kidney. There were 18 male rats (Rattus norvegicus) that used as the examination animal and were divided into three groups and were given the mixture of heated palm oil and polypropylene plastic per oral with intubation needle. The treatment was done for five weeks. The treatment for P0 group was 84 g of palm oil, for P1 was 84 g of palm oil with 2.3 g of plastic, and for the P2 was 84 g of palm oil with 6.8 g of plastic. The histopathological changes of the kidney were observed using microscope with 400x magnification and the data was analysed using Kruskal-Wallis and continued with Mann Whitney U test. The result showed that there were significant differences between three groups of treatment (P<0.05). There were effects of polypropylene plastic residue on heated palm oil against the histopathological changes of kidney in this research such as cast, epithelial sloughing and necrosis in the mild scale.

Co-Authors Afifatus Anindya Anggi Dwi Nugrahani Arif Nur Muhammad Ansori Astria Novitasari Nidom Balqis Afifah Brillian Inawulan Syamsiar Chairul Anwar Chairul Anwar Nidom Dewi Mariyam Dhani Prakoso Diyantoro Diyantoro Eka Pramyrtha Hestianah Endang Suprihati Eny Coolfina Simarmata Erma Safitri Faisal Fikri Fedik Abdul Rantam Hamid, Iwan Sahrial Hana Eliyani Hani Plumeriastuti Imam Mustofa Ira Sari Yudaniayanti Ira Sari Yudaniayati Jola Rahmahani Kadek Rachmawati Kusnoto Kusnoto Lianny Nangoi Lita Rakhma Yustinasari Maya Nurwartanti Yunita Meles, Dewa Ketut Mochamad Lazuardi Muhammad Khaliim Jati Kusala Muhammad Thohawi Elziyad Purnama Nor Laela Ramadhaniyah Nove Hidajati Putri Anggraheni Kusumaningrum Ratih Novita Praja Reviany Vibrianita Nidom Roesno Darsono Sarmanu Sarmanu Sartika Dewi Rachmani Setiawan Koesdarto Setyarina Indrasari Shabrina Fauzia Prayoga Shahani Azpriyanne Cahyono Soeharsono Soeharsono Sri Mumpuni Sosiawati Thomas Valentinus Widiyatno Tisya Yumn Al-Zuhroh

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