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All Journal Jurnal Ilmiah Perikanan dan Kelautan Jurnal Medik Veteriner J-Dinamika: Jurnal Pengabdian Kepada Masyarakat Ovozoa: Journal of Animal Reproduction journal of Basic Medical Veterinary
Epy Muhammad Luqman
Departemen Anantomi Veteriner Fakultas Kedokteran Hewan Universitas Aitlangga
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Immunology & microbiology Medicine & Pharmacology Veterinary

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Pengaruh Substitusi Artemia spp. dengan Keong Mas (Pomacea canaliculata) dan Cacing Tanah (Lumbricus rubellus) Terhadap Pertumbuhan dan Retensi Protein Benih Ikan Gabus (Channa striata) [Substitution Effect Of Artemia spp. With Golden Snail (Pomacea canaliculata) and Worm (Lumbricus rubellus) On The Growth and Protein Retention Snakehead Seed (Channa striata) ] Moch. Amin Alamsjah; Ditari Kurnia D.; Epy Muhammad Luqman
Jurnal Ilmiah Perikanan dan Kelautan Vol. 5 No. 2 (2013): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (83.026 KB) | DOI: 10.20473/jipk.v5i2.11400

Abstract

Abstract Freshwater culture commodity such as snakehead fish (C. striata) has high sufficient demand. Attempts for maintaining snakehead fish population (C. striata) has been done by cultivating. Natural feeding form Artemia spp. can increase the survival rate of snakehead fish seed (C. striata), but the usage of Artemia spp. make farmers pay high cost that it can cause loss. This study aimed to determine the effect of substitution of Artemia spp. with golden snails (Pomacea cancaliculata) and worm (Lumbricus rubellus) on the growth and protein retention of snakehead fish seed. Methods that used in this study is experimental method, with Completely Randomized Design (RAL) as an experimental design. Treatment is given in the form of nine different types of feed that includes, P0 treatment with Artemia spp., P1 with worm feed (L. rubellus), P2 with golden snail feed (P. cannaliculata), P3 with feed Artemia spp. 75% and worm (L. rubellus) 25%, P4 with feed Artemia spp. 50% and worm (L. rubellus) 50%, P5 with feed Artemia spp. 25% and worm (L. rubellus) 75%, P6 with feed Artemia spp. 75% and golden snail (P. cannaliculata) 25%, P7 with feed Artemia spp. 50% and golden snail (P. cannaliculata) 50%, P8 with feed Artemia spp. 25% and golden snail (P. cannaliculata) 75%. Each treatment was repeated three times. Data analysis using analysis of variance (ANOVA). The results showed that substitution Artemia spp. with golden snail (P. canniculata) with worm (L. rubellus) doesn’t give real effect (P>0.05) to the growth and protein retention of seed snakehead fish (C. striata). The average of daily growth rate (SGR) 1.60-1.889%, average absolute growth 1.2391.522cm and protein retention 40.664-49.406%. The results showed that golden snail (P. canniculata) and worm (L. rubellus) can be used as substitution feed of Artemia spp. for snakehead fish (C. striata).
Pengaruh injeksi nikotin terhadap motilitas, viabilitas, dan integritas membran spermatozoa mencit (Mus Musculus) Kautsar Pandu Pramudita; Widya Paramita Lokapirnasari; Suheni Susilowati; Hermin Ratnani; Erma Safitri; Epy Muhammad Luqman
Ovozoa : Journal of Animal Reproduction Vol. 9 No. 3 (2020): Ovozoa : Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v9i3.2020.77-81

Abstract

The process of spermatogenesis is sensitive to nicotine, a main component of cigarette smoke. This study aimed to observe the effect of nicotine injection on motility, viability, and membrane integrity of the spermatozoa of mice (Mus musculus). The mice were 10 weeks old, weighing about 20 grams, and in healthy and fertile conditions. Mice were adapted for 1 week by being given feed in the form of pellets and drinking water every day. Twenty of the mice were divided into four groups. Group K as a control was injected with 0.9% NaCl at 2.5 mg/Kg bw. Group P1 was injected with nicotine at 2.5 mg/Kg bw. P2 was injected with nicotine at 5 mg/Kg bw. Group P3 was injected with nicotine at 10 mg/Kg bw. The injection was carried out subcutaneously as much as 0.5 ml once a day for 35 days. Data analysis used the Analysis of Variances (ANOVA), followed by Duncan's Multiple Range Test. The results showed that the viability, motility, and membrane integrity of the spermatozoa decreased significantly (p <0.05) according to the increase in the dose of nicotine injected. It could be concluded that subcutaneous nicotine injection linearly decreased the motility, viability, and membrane integrity of the spermatozoa of mice.
Probiotic Potential of Lactic Acid Bacteria on Feed Efficiency, Weight and Carcass Percentage in Ducks Evania Haris Chandra; Widya Paramita Lokapirnasari; Sri Hidanah; Muhammad Anam Al-Arif; Wiwik Misaco Yuniarti; Epy Muhammad Luqman
Jurnal Medik Veteriner Vol. 5 No. 1 (2022): April
Publisher : Faculty of Veterinary Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol5.iss1.2022.69-73

Abstract

The aim of the study was to determine the effect of the use of probiotic lactic acid bacteria (Lactobacillus acidophilus, Lactobacillus casei, Lactococcus lactis, and Bifidobacterium sp) on feed efficiency, carcass weight and carcass percentage in broiler ducks. Probiotics lactic acid bacteria produce bacteriocins and organic acids that inhibit the growth of pathogenic microbes in the digestive tract of poultry, so that the intestinal villi of broiler ducks are healthy. Besides that, probiotics are able to reduce cholesterol levels in meat when consumed by humans and can improve health. This study used 48 peking ducks. The probiotics of Lactic Acid Bacteria used were a concentration of 1.2 x 108 CFU/ml. In this study there were four different treatments, namely P0 (without probiotics), P1 (given 1 ml probiotics/liter drinking water), P2 (given 2 ml probiotics/liter drinking water) and P3 (given 3 ml probiotics/liter of drinking water). The results of the feed efficiency study showed that there was a significant difference (p<0.05) in the P0, P1, P2 and P3 treatments. The highest feed efficiency was at P3 (33.81%) while the lowest was P0 (30.84%). The results of the study on carcass weight indicated that the probiotics tended to be higher than those who were not given probiotics but statistically not significantly different (p>0.05). The highest carcass weight was P3 (1749.92 g) and the lowest was P0 (1709.25 g). The results of the study on the percentage of carcass weight showed a significant difference (p<0.05) in both P0, P1, P2 and P3 treatments. The highest percentage of carcass weight was at P3 (64.60%) and the lowest was P0 (60.96%). It can be concluded that the administration of probiotics lactic acid bacteria (Lactobacillus acidophilus, Lactobacillus casei, Lactococcus lactis, and Bifidobacterium sp) at a dose of 1 ml/liter of drinking water, 2 ml/liter of drinking water and 3 ml/liter of drinking water can affect feed efficiency and the percentage of duck carcasses. but has no effect on the carcass weight of broiler ducks.
Penerapan Teknologi Reproduksi Inseminasi Buatan untuk Meningkatkan Produktivitas Reproduksi dan pemberdayaan Peternak Itik di Kecamatan Candi Kabupaten Sidoarjo Rosa Tri Hertamawati; Epy Muhammad Luqman
J-Dinamika : Jurnal Pengabdian Masyarakat Vol 5 No 2 (2020): Desember
Publisher : Politeknik Negeri Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25047/j-dinamika.v5i2.2416

Abstract

Kecamatan Candi di Kabupaten Sidoarjo merupakan sentra produksi telur itik yang memerlukan diversifikasi usaha untuk mengatisipasi harga pasar telur itik tidak stabil.Usaha pembibitan melalui teknologi Inseminasi Buatan (IB) merupakan salah satu diversifikasi produkuntuk menghasilkan telur tetas sebagai upaya untuk menghasilkan DOD yang mempunyai daya tetas tinggi dan meningkatkan efisiensi pejantan. Pengabdian masyarakat ini dilaksanakan di Kecamatan Candi Kebupaten Sidoarjo selama 2 bulan yang terbagi dalam dua tahap yaitu : Pada tahap I dilakukan interview dan wawancara ntuk mengetahui latar belakang pengetahuan, minat dan kemampuan dalam para peternak inseminasi buatan pada itik.Pada tahap IIdiberikan pelatihanpada peternak dan mengevaluasi pengetahuan dan kemampuan secara teoritis dalam inseminasi buatan pada itik. Evaluasi untuk mengetahui kemampuan dan minat peternak, evaluasi ini dilakukan selama kegiatan berupa kuisioner untuk pre dan post test. Pemberdayaan melalui penyuluhan dan pelatihan telah mampu meningkatkan pengetahuan dan kemampuan peternak itik di Kelompok Peternak Itik sebesar 14,74 % dan sebagian besar peternak itik (73,68 %) berminat menerapkan teknologi IB pada itik sebagai upaya efisiensi penggunaan pejantan itik, meningkatkan fertilitas dan daya serta kualitas anak itik dalam rangka meningkatkan pendapatan.
EFEK PEMBERIAN EKSTRAK RUMPUT KEBAR (Biophytum petersianum Klotzsch) TERHADAP JUMLAH SEL LEYDIG MENCIT (Mus musculus) JANTAN YANG DIPAPAR 2,3,7,8-TETRACHLORODIBENZO-P-DIOXIN Milla Nursadida; Hani Plumeriastuti; Yeni Dhamayanti; Widjiati Widjiati; Epy Muhammad Luqman; Arimbi Arimbi
Journal of Basic Medical Veterinary Vol. 8 No. 1 (2019): Journal of Basic Medical Veterinary, June 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i1.19998

Abstract

This study was aim to examine the effect of kebar grass extract (Biophytum petersianum Klotzsch) to against number leydig cells of mice (Mus musculus) by exposed 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD). Twenty five male mice (Mus musculus) 4 months with avarage body weight 20 g were used. These animals were divided into five groups (K(-), K(+), P1, P2 and P3). K(-) was treated with placebo, K(+) was treated by exposed TCCD single dose injection intaperitoneal 7µg/KgBw, P1 was treated by exposed TCCD single dose injection intaperitoneal 7µg/KgBw and kebar grass extract 0,045 mg/g Bw/day P2 was treated by exposed TCCD single dose injection intaperitoneal 7µg/KgBw and kebar grass extract 0,080 mg/g Bw/day, P3 was treated by exposed TCCD single dose injection intaperitoneal 7µg/KgBw and kebar grass extract 1,350 mg/g Bw/day. This research has been conducted for 53 days. The data were compared using ANOVA and Duncan test by SPSS 22.4 for windows. The result showed that Kebar Grass Extract in all of groups can prevent the damage of leydig cells in testis that exposed by TCCD significantly (p<0,05) and kebar grass extract 0,135 mg/kgBw/day can increase amount of leydig cells maximaly.
PENGARUH PEMBERIAN EKSTRAK KULIT PISANG KEPOK (Musa acuminata) TERHADAP GAMBARAN HISTOPATOLOGI BRONKUS DAN VENA PULMONALIS MENCIT (Mus musculus) JANTAN YANG DIPAPAR ASAP ROKOK Winny Jeanita; Dewa Ketut Meles; Widjiati WIdjiati; Iwan Sahrial Hamid; Epy Muhammad Luqman; Arimbi Arimbi
Journal of Basic Medical Veterinary Vol. 9 No. 1 (2020): Journal of Basic Medical Veterinary, June 2020
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v9i1.21089

Abstract

This research aimed to attest the effect of Kepok banana (Musa acuminata) peel extract on the histopathology of bronchus and pulmonary veins in male mice (Mus musculus) exposed by cigarette smoke. The 24 experimental male mice were divided into six groups with each treatment having four replicate. All the groups, except K- as a control group, was treated with cigarette smoke for 14 days. After that, K- and K+ was treated with CMC-Na 1%, KO was treated with ascorbic acid 13 mg/kgBW, P1 was treated with Kepok banana peel extract 14 mg/kgBW, P2 was treated with Kepok banana peel extract 28 mg/kgBW, and P3 was treated with Kepok banana peel extract 56 mg/kgBW for 14 days. The data of the thickness of bronchial epithelium and the diameter of pulmonary veins were analyzed using ANOVA (Analysis of Varience) with significance p<0,05 and continued with Post- hoc Tukey. The result of the thickness of bronchial epithelium showed that K+ had the highest thickening (431,83 + 9,71 µm) and significant differences (P<0,05) with all the groups. The result of P3 showed the lowest thickening of bronchial epithelium (170,84 + 2,70 µm). The result of pulmonary veins diameter showed that K+ had the narrowest diameter (525,64 + 16,61 µm) and significant differences (p<0,05) with all the groups. The result of P3 showed the lowest diameter narrowing of pulmonary veins (718,10 + 9,64 µm). The conclussion of this research are Kepok banana peel extract able to decreased the thickness of bronchial epithelium and the narrowing of pulmonary veins diameter in male mice with effective dose 28 mg/ kgBW.
PENGARUH PEMAPARAN KARBOFURAN PADA INDUK MENCIT (Mus musculus) TERHADAP GAMBARAN HISTOPATOLOGI GINJAL ANAK MENCIT MASA LAKTASI Edi Purnomo; Epy Muhammad Luqman; Hermin Ratnani; Hani Plumeriastuti; Maslichah Mafruchati; Yeni Dhamayanti
Journal of Basic Medical Veterinary Vol. 9 No. 1 (2020): Journal of Basic Medical Veterinary, June 2020
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v9i1.21095

Abstract

Carbofuran is a broad spectrum carbamat insecticide which is used in various farm practices. An exposure of carbofuran has been reported able to induce Reactive Oxygen Species (ROS) or free radical in kidney. Free radical caused cells damage. The present study was designed to investigate the effect of carbofuran in kidneys histopathologic of mice pup whose dam were exposed by carbofuran during the lactation period. High levels of ROS can bind to various biomolecules such as proteins, lipids and nucleic acids so that carbofuran has a negative effect on tissue functions and structures. Free radical caused changes in the pathological conditions of the tissue including the kidneys. The dams were devided into four groups of five animals each. P0 animal group was received aquadest 0,1 ml per body weight (BW), P1 animal group was treated carbofuran at the dose of 1/16 LD50 (0,3125 mg per BW), P2 animal group was treated carbofuran at the dose of 1/8 LD50 (0, 625 mg per BW), and P3 animal group was treated carbofuran at the dose of 1/4 LD50 (1,25 mg per BW). The carbofuran was exposed via oral postnatal day 1st until 9th postnatal day after delivery. At postnatal day 10th, offspring were sacrified. The kidney of mice pup was collected and processed for microscopic examination with hematoxilin-eosin staining. Microscopic observations on the histology of the mice pup kidney showed an increase in the degree of kidney damage as an increase in the dose of carbofuran given to the dams. The results showed tubular changes in the form of tubular degeneration, inflammatory cell infiltration and tubular necrosis in the kidney of mice pups.
Green tea (Camellia sinensis) leaf extract maintained spermatozoa plasma membrane integrity, viability, and motility of mice (Mus musculus) exposed to cigarette smoke Maryana Siska Silviani; Moh. Sukmanadi; Rochmah Kurnijasanti; Sri Pantja Madyawati; Epy Muhammad Luqman
Ovozoa : Journal of Animal Reproduction Vol. 11 No. 3 (2022): Ovozoa : Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v11i3.2022.130-136

Abstract

Cigarette smoke chemicals caused oxidative stress by increasing Reactive Oxygen Species (ROS). ROS exposure could be neutralized by antioxidants, such as green tea (Camellia sinensis) leaf extract. This study aimed to determine the effect of green tea extract (GTE) on spermatozoa plasma membrane integrity, spermatozoa viability, and spermatozoa motility of mice (Mus musculus) exposed to cigarette smoke. Twenty-five mice were randomly divided into five groups. Group C- mice were given a placebo (1% Sodium carboxymethyl cellulose, Na-CMC). Group C+, T1, T2, and T3 mice were exposed to cigarette smoke and given 0, 20, 40, and 60 mg/kg BW GTE respectively. Cigarette smoke exposure used a clove cigarette per day. GTE in 1% Na-CMC solution was administered at 0.5 mL orally using a gastric probe. The treatment was conducted daily for 36 days, and on day 37, all mice were euthanized for spermatozoa evaluation. The results showed that all parameters evaluated in the C+ group were lower (p <0.05) than in C- group. Administration of GTE in the T2 group increased (p <0.05) all parameters compared to mice in the C+ group. However, administration of GTE to mice in the T3 group caused a decrease (p <0.05) in all parameters than those of the T2 group and was not significantly different (p >0.05) compared to those of the C+ group. It could be concluded that the administration of GTE at 40 mg/kg BW has maintained the spermatozoa plasma membrane integrity, spermatozoa viability, and spermatozoa motility of mice exposed to cigarette smoke.
Co-Authors Arimbi Arimbi Ditari Kurnia D. Edi Purnomo ERMA SAFITRI Evania Haris Chandra Hamid, Iwan Sahrial Hani Plumeriastuti Hermin Ratnani Hermin Ratnani Hertamawati, Rosa Tri Kautsar Pandu Pramudita Maryana Siska Silviani Maslichah Mafruchati Meles, Dewa Ketut Milla Nursadida Mochammad Amin Alamsjah Moh. Sukmanadi Muhammad Anam Al-Arif Rochmah Kurnijasanti Sri Hidanah Sri Pantja Madyawati Suheni Susilowati Widjiati Widjiati Widya Paramita Lokapirnasari Winny Jeanita Wiwik Misaco Yuniarti Yeni Dhamayanti