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All Journal journal of Basic Medical Veterinary
Wiwik Tyasningsih
Fakultas Kedokteran Hewan Universitas Airlangga
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

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IDENTIFIKASI BAKTERI Escherichia coli PENGHASIL Extended Spectrum β-Lactamase DARI SWAB RECTAL SAPI PERAH MENGGUNAKAN METODE VITEK-2 DI KUD TANI WILIS SENDANG KABUPATEN TULUNGAGUNG Akyun Rozaqi Syah Putra; Mustofa Helmi Effendi; Setiawan Koesdarto; Suwarno Suwarno; Wiwik Tyasningsih; Agnes Theresia Soelih Estoepangestie
Journal of Basic Medical Veterinary Vol. 8 No. 2 (2019): Journal of Basic Medical Veterinary, December 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i2.20414

Abstract

Antibiotic resistance in animals and humans has become a global problem that needs attention. The use of antibiotics in inappropriate on food-producing animals can lead to resistance many of the pathogenic bacteria to the various types of antibiotics, one of which is the Escherichia coli (E. coli) which produces extended spectrum β-lactamase (ESBL). The aim of this study was to isolate and identified ESBL- E. coli isolate from dairy cow rectal swabs in Sendang, Tulungagung district using the Vitek-2 method. The number of rectal swab samples used in the present study was 50. The result of the study showed that from all of the samples could be isolated and indentified E. coli, based on the colony characteristics on EMBA and biochemical test. Based on the double disc synergy test method using antibiotic disc amoxicylyn-clavulanate, ceftriaxone, aztreonam, ceftazidime and cefotaxime, 10 isolates could be identify els ESBL- E. coli. furthermore 3 out of 10 isolates DDST positives were confirmed ESBL- E. coli using Vitek-2 method.
Co-Authors Agnes Theresia Soelih Estoepangestie Akyun Rozaqi Syah Putra Mustofa Helmi Effendi Setiawan Koesdarto Suwarno Suwarno