Rahmat Aditya Sumardi
Program Studi Pendidikan Dokter Hewan, Fakultas Kedokteran Hewan, Universitas Syiah Kuala, Banda Aceh

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Gambaran histologi ovarium sapi aceh pascavitrifikasi menggunakan dimetyl sulfoksida dengan konsentrasi berbeda . Syafruddin; Arman Sayuti; Rahmat Aditya Sumardi; Budianto Panjaitan
ARSHI Veterinary Letters Vol. 2 No. 4 (2018): ARSHI Veterinary Letters - November 2018
Publisher : School of Veterinary Medicine and Biomedical Sciences, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (333.431 KB) | DOI: 10.29244/avl.2.4.77-78

Abstract

This study aims to determine the morphology of Aceh bovine ovarian pasca vitrification, after its been exposed to dimetyl sulfoksida (DMSO) cryoprotectant. This study use a completely randomized design of one-way pattern ANOVA acquire three replications. Ovaries used in this study are Aceh cattle ovarian which collected from slaughter house (RPH) accounted as 4 organs, to the next sliced into 9 pieces. Ovarian pieces are then grouped into 3 treatment groups namely ovaries which exposed into solution containing DMSO 10% (P1), 20% (P2), and 30% (P3). The results showed that the average number of normal follicles is highest at P1 41,33 ± 32,51; followed by P2 20,00 ± 16,09 , and P3 15,66 ± 10,50. It was concluded that  the ovarian tissue was exposed with DMSO 10% was able to maintain of the normal follicle than DMSO 20% and 30% although statistical test results showed no significant difference between group (P>0,05).