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All Journal ARSHI Veterinary Letters Veterinary Biomedical and Clinical Journal
Dodik Prasetyo
Fakultas Kedokteran Hewan Universitas Brawijaya
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Health Professions Immunology & microbiology Veterinary

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Peneguhan diagnosa scabiosis metode sitologi kulit pada kucing domestik di Kota Malang Dodik Prasetyo; Indah Amalia Amri; Sri Murwani; Dahliatul Qosimah
ARSHI Veterinary Letters Vol. 3 No. 2 (2019): ARSHI Veterinary Letters - Mei 2019
Publisher : School of Veterinary Medicine and Biomedical Sciences, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avl.3.2.27-28

Abstract

Hewan harus terbebas dari rasa sakit dan penyakit, merupakan salah satu aspek dari “Five Freedom of Life”. Kucing domestik liar banyak dijumpai di kota Malang, baik di jalanan dan pasar yang diduga terkena Scabiosis. Kegiatan pengabdian masyarakat ini diharapkan dapat meningkatkan kesadaran, kepedulian, dan pengetahuan masyarakat terhadap kesehatan hewan melalui pelayanan pemeriksaan fisik kesehatan dan pemeriksaan penunjang dengan metode sitologi kulit. Teknik scrapping kulit digunakan untuk peneguhan diagnosa Scabiosis sebagai dasar untuk penanganan dan pengobatan. Kucing yang positif menderita scabiosis dilakukan tindakan kuratif, preventif dan pemberian supportif. Hasil sitologi pada 85 ekor kucing menunjukkan sebanyak 38 ekor kucing liar positif ditemukan parasit dan 47 lainnya tidak ditemukan parasit.
The Analysis of Pancreatic Duodenal Homeobox 1 (PDX-1) Gene Mutation Based on Blood Glucose Level on Domestic Cats (Felis catus) Post Ovaryohisterectomy Hana Mitsuki Putri; Aulani'am Aulani'am; Ajeng Aeka Nurmaningdyah; Dodik Prasetyo; Fajar Shodiq Permata
Veterinary Biomedical and Clinical Journal Vol. 3 No. 2 (2021)
Publisher : Faculty of Veterinary Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.VetBioClinJ.2021.003.02.5

Abstract

Ovariohysterectomy (OH) in domestic cats causes several side effects, including obesity. Obesity is closely related to diabetes. The glucoregulatory gene that will cause insulin resistance if there is a gene mutation is the PDX-1 gene. This insulin resistance will have a very immediate effect on blood glucose in the body. The study examined the relationship between PDX-1 gene polymorphisms and blood glucose levels in domestic cats with OH. The method used in this study was in vitro PCR amplification of the PDX-1 gene with Forward primers 'CCG AGC TGT CAA AGC TAG CG' and Reverse 'GAA CCG GTA GAG GGT CTT GG' and followed by sequencing of PCR products. The results showed fundamental differences in the base sequence in the PDX-1 gene sequences at c.435G>A, c.927A>T, and c.584A>G. The fundamental difference in the PDX1 gene sequence is a silent mutation
Co-Authors Ajeng Aeka Nurmaningdyah Aulani'am Aulani'am Fajar Shodiq Permata Hana Mitsuki Putri Indah Amalia Amri Qosimah, Dahliatul Sri Murwani