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All Journal KOVALEN: Jurnal Riset Kimia Wikrama Parahita : Jurnal Pengabdian Masyarakat FLUIDA J-Dinamika: Jurnal Pengabdian Kepada Masyarakat
Sudrajat Harris Abdulloh
Jurusan Teknik Kimia, Politeknik Negeri Bandung
Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Economics, Econometrics & Finance Education Energy Engineering Environmental Science Industrial & Manufacturing Engineering Materials Science & Nanotechnology Social Sciences

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Journal : FLUIDA

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Aktivitas Bacillus subtilis pada Media Tulang Ayam dalam Pembentukan Gelatin Sudrajat Harris Abdulloh; Bintang Iwhan Moehady; Irwan Hidayatulloh
Fluida Vol 13 No 1 (2020): FLUIDA
Publisher : Jurusan Teknik Kimia, Politeknik Negeri Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35313/fluida.v13i1.2221

Abstract

ABSTRAK Gelatin merupakan bahan tambahan yang banyak terdapat pada industri makanan, minuman, dan tekstil. Gelatin terbentuk secara kimia melalui proses asam dan basa atau dengan bantuan aktivitas mikroorganisme. Proses kimia pembentukan gelatin membutuhkan waktu antara satu sampai lima minggu, sedangkan proses dengan aktivitas mikroorganisme terbentuk antara 12 sampai 24 jam. Penelitian ini bertujuan untuk membuat gelatin dengan menggunakan bakteri Bacillus subtilis ATCC No. 21228 serta bahan baku tulang ayam. Aktivitas bakteri Bacillus subtilis akan merombak kolagen dalam tulang ayam menjadi gelatin. Kondisi operasi yang digunakan yaitu: temperatur 45, 50, dan 55°C; pH 7, 8, dan 9; dan konsentrasi 120 g/L, 160 g/L, 200 g/L dan 240 g/1. Analisis terhadap produk yang diperoleh menggunakan uji kandungan protein dan uji pembentukan gelatin. Hasil penelitian menunjukkan kondisi optimum terjadi pada pH = 9, T = 45°C, konsentrasi (C) = 200 g/L, dan konversi rata-rata = 72,25%. Kata kunci: Gelatin, Bacillus subtilis, limbah tulang ayam ABSTRACT Gelatin is an additive that is widely available in the food, beverage, and textile industries. It is formed chemically through acid and alkaline processes or with the help of microorganism activity. The chemical process for gelatin takes 1-5 weeks, while the process with microorganism activity occur only 12-24 hours. This study aims to make gelatin using the bacteria Bacillus subtilis ATCC No.21228 from raw material for chicken bones. The activity of the Bacillus subtilis bacteria will convert collagen in chicken bones into gelatin. The operating conditions used in this work were: temperature under 45, 50, and 55°C; pH between 7, 8, and 9; and concentrations of 120 g/L, 160 g/L, 200 g/L and 240 gr/1. The result tested to obtain the existing protein and gelatin content. The results showed that the optimum conditions occurred at pH = 9, T = 45°C, concentration = 200 g/L, and the average conversion = 72.25%. Keywords: Gelatin, Bacillus subtilis, chicken bone waste
Seeding and Acclimatization for Aerobic Processing of Restaurant Wastewater with Sequencing Batch Reactor Herawati Budiastuti; Laily Isna Ramadhani; Sudrajat Harris Abdulloh; Ananda Dea Maharani; Melina Melina; Robby Sudarman
Fluida Vol 16 No 1 (2023): FLUIDA
Publisher : Jurusan Teknik Kimia, Politeknik Negeri Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35313/fluida.v16i1.4521

Abstract

Restaurant wastewater has a relatively high organic matter content, so it needs to be treated to meet the specified quality standard. One of the technologies that can be used in restaurant wastewater treatment is Sequencing Batch Reactor (SBR) technology. The purpose of this study is to set up an aerobic SBR system with seeding and acclimatization treatments to reduce the amount of organic matter in restaurant wastewater when a shock load occurs. The research was done using wastewater from a restaurant in Bandung and activated sludge from the food industry in Bogor as seeds for microorganisms. In this study, the seeding process was carried out by introducing 25% activated sludge and 75% nutrients into the reactor, and the acclimatization process was carried out by introducing a specific ratio of nutrients and wastewater into the reactor gradually until the waste concentration reached 100%. The parameters tested were COD, MLVSS, DO, pH, and temperature. During the seeding procedure, the initial COD value of 3,200 mg/L declined. It began to stabilize on the seventh day, with a COD value of 1,080 mg/L. The COD removal reached a relatively stable condition in the acclimatization process starting on day 2, where COD decreased from the original 1,280 mg/L to 480 mg/L.
Co-Authors Alfiana Adhitasari Ananda Dea Maharani Bambang Soeswanto Bintang Iwhan Moehady Emma Hermawati Herawati Budiastuti Hidayatulloh, Irwan Lili Indrawati Melina Melina Mentik Hulupi Nancy Siti Djenar Nurcahyo, Nurcahyo Ramadhani, Laily Isna Retno Indarti Riniati Rispiandi Rispiandi Robby Sudarman Setyaningrum, Sinta Sihombing, Rony Pasonang Troskialina, Lina Widyabudiningsih, Dewi Yunus Tonapa Sarungu Yusuf Sofyan