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All Journal Reaktor Bioma : Berkala Ilmiah Biologi Jurnal Natur Indonesia Biopropal Industri JURNAL BIOLOGI INDONESIA Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology ANNALES BOGORIENSES Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Teknologi Indonesia
Ahmad Thontowi
Research Center for Biotechnology-LIPI
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Education Energy Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology

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Pertumbuhan Bakteri Laut Shewanella indica LBF-1-0076 dalam Naftalena dan Deteksi Gen Naftalena Dioksigenase - (The Growth of Marine Bacteria Shewanella indica LBF-1-0076 in Naphthalene and Naphthalene dioxygenase Gene Detection) Farini, Nuzul; Thontowi, Ahmad; Yetti, Elvi; Suryani, Suryani; Yopi, Yopi
Biopropal Industri Vol 8, No 1 (2017)
Publisher : Balai Riset dan Standardisasi Industri Pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (148.664 KB)

Abstract

Crude oil exploitation which often occured offshore can cause water pollution in the sea since its contains naphthalene which is a hazardous compounds. This research used marine bacteria LBF-1-0076 that have ability in naphthalene degradation. This research aimed to study the parameter effect of naphthalene and cell concentration toward marine bacteria LBF-1-0076. This research also identified isolate LBF-1-0076 and detected the encode gene of naphthalene dioxygenase. Based on growth test result, the optimum naphthalene degradationby isolate LBF-1-0076 occured in 75 ppm naphthalene concentration with 15cell concentration. The result of 16S rDNA gene analysis showed that LBF-1-0076 was identified as Shewanella indica strain 0102 with identical value 99%. The result of naphthalene dioxygenase gene detection using Polymerase Chain Reaction (PCR) showed that the isolate contained naphthalene dioxygenase gene with size ±377 bp. Therefore, LBF-1-0076 potential as bioremediation agent to solve crude oil contamination in the sea.Keywords:   crude oil, marine bacteria, naphthalene, naphthalene dioxygenase, Shewanella indicaABSTRAKEksploitasi minyak bumi yang sering terjadi di laut mengakibatkan adanya pencemaran minyak di laut. Naftalena merupakan salah satu senyawa dominan berbahaya yang terkandung dalam minyak bumi dan dapat mengakibatkan pencemaran perairan. Penelitian ini menggunakan bakteri laut LBF-1-0076 yang memiliki kemampuan untuk mendegradasi naftalena. Tujuan dari penelitian ini adalah mempelajari pengaruh parameter konsentrasi naftalena dan konsentrasi sel terhadap bakteri laut pendegradasi naftalena LBF-1-0076. Penelitian ini juga bertujuan untuk mengidentifikasi isolat LBF-1-0076 dan mendeteksi gen pengkode naftalena dioksigenase. Berdasarkan hasil uji pertumbuhan, degradasi naftalena yang optimal oleh isolat LBF-1-0076 terjadi pada konsentrasi naftalena 75 ppm dengan konsentrasi sel 15. Hasil analisis gen 16S rDNA menunjukkan isolat LBF-1-0076 teridentifikasi sebagai Shewanella indica strain 0102 dengan nilai keidentikan 99%. Hasil deteksi gen naftalena dioksigenase dengan menggunakan Polymerase Chain Reaction (PCR) menunjukkan bahwa isolat tersebut mempunyai gen naftalena dioksigenase dengan ukuran ±377 bp. Oleh karena itu, isolat LBF-1-076 berpotensi sebagai agen bioremediasi untuk mengatasi masalah pencemaran minyak bumi di laut.Kata kunci: bakteri laut, minyak bumi, naftalena, naftalena dioksigenase, Shewanella indica
Pertumbuhan Optimal Bakteri Laut Pseudomonas aeruginosa LBF-1-0132 dalam Senyawa Piren Safitriani, Safitriani; Thontowi, Ahmad; Yetti, Elvi; Suryani, Suryani; Yopi, Yopi
JURNAL BIOLOGI INDONESIA Vol 13, No 1 (2017): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v13i1.3100

Abstract

ABSTRACTPyrene is a high molecular weight chemical compound belongs to polycyclic aromatic hydrocarbon (PAHs) group that are difficult to degrade by environment. Biodegradation techniques using indigenous marine bacteria are used to be as an effort to reduce pollutants that are carsinogenic. The objectives of this research are to screen of 18 marine bacteria isolates qualitatively by sublimation method and quantitatively by growth test and to optimize degradation activity of marine bacteria isolates by pyrene concentration and cell concentration. Identification by 16S rDNA and phylogenetic tree analysis were conducted to determine the molecular basis of bacterial identity. The result of sublimation showed that 15 isolates were positive result for pyrene degradation and classified to 3 groups. The first group consisted of 5 isolates that can produce clear zone, while the second group are 5 isolates with isolate color changes. The third group have both of activities. Growth test showed that isolate LBF-1-0132 has high potency to degrade pyrene compound. Isolate LBF-1-0132 is capable of degrading pyrene compounds optimally at concentration of 600 ppm and optimum cell concentration of 20. Based on 16S rDNA gene analysis, isolate LBF-1-0132 is Pseudomonas aeruginosa with 98% identity.Keywords :pyrene, marine bacteria, optimization, 16S rDNA identification
Pencirian Produksi Amilase oleh Saccaromyces cerevisiae W303A Rekombinan Thontowi, Ahmad; Puspaningsih, Ni Nyoman Tri; Hadi, Sofjan; Purkan, Purkan; Irawan, Bambang
JURNAL BIOLOGI INDONESIA Vol 3, No 3 (2002): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (398.112 KB) | DOI: 10.14203/jbi.v3i3.3464

Abstract

ABSTRACTCharacterization of Amylase Production by Saccharomyces cerevisiae W303A Recombinants. Cloning of amylase gene from Endomycopsis fibuligera ITB.R.cc.64 into S. cerevisiae W303a can effectively increase the yeast function to digest starch directly into ethanol. Production of amylase by S. cerevisiae W303a recombinants (I and P) were done by growing in yeast peptone starch (YPS) medium. The result showed that the recombinants could be produced of amylase by gave clear zone after staining by iodium vapor. The optimum condition of production of amylase by S. cerevisiae W303a recombinants were pH 7.0, 40?C temperature incubation, and gave maximum activity after 36 hours incubation. Amylase activity of I was higher than P recombinant for these condition respectively.Key words: Characterization, amylase, S. cerevisiae W303a
Metabolisme Benzonitril oleh Flavobacterium sp. NUB 1 Sulistinah, Nunik; Sunarko, Bambang; Thontowi, Ahmad
JURNAL BIOLOGI INDONESIA Vol 3, No 3 (2002): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (339.945 KB) | DOI: 10.14203/jbi.v3i3.3472

Abstract

ABSTRACTMetabolism of Benzonitriles by Flavobacterium sp. NUB 1. Flavobacterium sp. NUB 1 was isolated from industrial waste of PT. Petrokimia Gresik. The bacterium was able to utilize benzonitrile and acetonitrile and propionitril as the sole source of carbon and nitrogen. Growth on benzonitrile gave higher growth rate and biomass yield than growth on acetonitrile and propionitrile. When Flavovobacterium sp. NUB1 grew on benzonitril 15 mM , the doubling time is 9 hours 54 minutes and the specific growth rate (?) was 0,07 h-1. Whole cell of Flavobacterium sp. NUB 1 could hydrolyzed aromatic and aliphatic nitriles. The bacteria isolate has ability in metabolism of acetonitrile greater than benzonitrile. Activity of nitrile hydratase and amidase are more dominant than nitrilase in metabolism of benzonitrile.Key words: Biodegradation, benzonitril, Flavobacterium sp. NUB 1, nitrile-hydratase,amidase, nitrilase
Karakterisasi Biodegradasi Senyawa Poliaromatik Dibenzothiophene Oleh Bakteri Laut Novosphingobium mathurense LBF-1-0061 Tanjung, Puspasari Noerwan; Yetti, Elvi; Thontowi, Ahmad; Suprihadi, Agung; Purwantisari, Susiana; Yopi, Yopi
JURNAL BIOLOGI INDONESIA Vol 12, No 2 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1056.931 KB) | DOI: 10.14203/jbi.v12i2.2894

Abstract

ABSTRACTDibenzothiophene is one of polycyclic aromatic hydrocarbon (PAH) compound containing sulfur element. This compound has toxicity, mutagenic and quiet persistent in environment. From sreening test, it was known that isolate LBF-1-0061 was potential to degrade dibenzothiophene. The objectives of this study are to study dibenzotiophene degrading capability by marine bacteria isolate LBF-1-0061 using screening test; analysis of dibenzothiophene residue by GC/MS and identifiy the isolate by molecular identification. The result of this research shown that LBF-1-0061 isolate could grow up to 100 ppm of dibenzotiophene. This isolate also presented degrading capability approximately 37.5% of dibenzotiophene in 14 days incubation. Based on partial 16S rRNA gene analysis, LBF-1-0061 was identified 99% as Novosphingobium mathurense strain SM117.Keywords: sea bacteria, biodegradation, dibenzotiofen, hydrocarbon aromatic polisiclic
Keragaman Bakteri Laut Pendegradasi Alkana dan Poliaromatik Hidrokarbon di Pulau Pari Jakarta Thontowi, Ahmad; Yopi, Yopi
JURNAL BIOLOGI INDONESIA Vol 9, No 1 (2013): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (185.283 KB) | DOI: 10.14203/jbi.v9i1.154

Abstract

Minyak mentah merupakan salah satu sumber pencemaran di lingkungan laut. Degradasi oleh bakteri memegangperanan penting dalam bioremediasinya. Sejumlah 66 bakteri laut dari Pulau Pari, Kepulauan Seribu Jakarta telahdiisolasi, dianalisa berdasarkan gen 16S rDNA, dan diuji kemampuannya dalam mendegradasi minyak. Berdasarkananalisis gen 16S rDNA diperoleh lima kelompok bakteri pendegradasi minyak, yaitu α-proteobakteria (43.6%), γ-proteobakteria (48.5 %), Flavobakteria (4.5 %), Aktinobakteria (1,5 %), dan Bacillales (1,5%). Bakteribakteritersebut mampu mendegradasi komponen minyak (senyawa alkana dan poliaromatik hidrokarbon). γ-Proteobakteria dan α-proteobakteria mempunyai peran penting dalam bioremediasi minyak di kawasan lingkunganlaut di Pulau Pari. Dari hasil tersebut membuktikan bahwa bakteri pendegradasi minyak dari Pulau Pari sangatberagam.Kata kunci: minyak, laut, bakteri, bioremediasi, alkana, poliaromatik hidrokarbon
Evaluation of Non-Saccharomyces Cerevisiae Strains Isolated from Sea Water Against Inhibitory Compounds for Ethanol Production Thontowi, Ahmad; Putra, Filemon Jalu Nusantara; Yopi, Yopi
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 12, No 2 (2017): August 2017
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v12i2.284

Abstract

An important parameter in industrial bioethanol fermentation is the resistance of yeast to osmotic pressure and inhibitor compounds. Aureobasidium pullulans LBF-3-0074 and Schwanniomyces etchellsii LBF-3-0034 are reported capable to produce ethanol. LBF-3-0034 and LBF-3-0074 are yeast strains isolated from Bali and Lombok sea water. This study aimed to evaluate characteristics of both LBF-3-0034 and LBF-3-0074 strains under the effects of glucose and inhibitor compounds. Both strains were allowed to consume glucose up to 120 mM. Then, these strains were grown with the present of several inhibitors, i.e. 5-hydroxymethyl-2-furaldehyde (5-HMF), furfural, acetic acid, formic acid, and levulinic acid. Results showed that the two yeast strains studied could grow and ferment the sugars under both osmotic and inhibitor stress conditions. As conclusion, Schwanniomyces etchellsii LBF-3-0034 and Aureobasidium pullulans LBF-3-0074 are potential for direct fermentation of lignocellulosic hydrolysate to ethanol.
Optimization of Substrate and Starter Cell Concentrations for Dibenzothiopene Biodegradation by Indigeneous Marine Bacteria Mauricauda olearia LBF-1-0009, Alcanivorax xenomutants LBF-1-0018, and Stakelama pacifica LBF-1-0031 Yetti, Elvi; Thontowi, Ahmad; Yopi, Yopi
ANNALES BOGORIENSES Vol 21, No 2 (2017): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (646.12 KB) | DOI: 10.14203/ab.v21i2.300

Abstract

Dibenzothiophene (DBT) and its derivatives have been widely used as model organic sulfur compounds in petroleum, included their biodegradation process. The abilities of microorganisms to degrade pollutants are significantly influenced by various factors such as microbial species, nutrients and environmental parameters. In this research, we carried out further study to determine optimal condition for DBT biodegradation regarding with substrate and strains cell concentration by several indigenous marine bacteria from Indonesia. These three isolates were belong to Mauricauda olearia, Alcanivorax xenomutants, and Stakelama pacifica, with homology result 99% each. Optimal dibenzothiophene as substrate reached by all isolates is 100 ppm, while cell concentration or microbial numbers that gave highest growth for all isolates is 20 based on conversion of OD600 nm measurement.  
Polyaromatic Hydrocarbon Degradation and Dioxygenase Gene Detection from Alteromonas alvinellae Bt05 Thontowi, Ahmad; Rahmani, Nanik; Yopi, Yopi
ANNALES BOGORIENSES Vol 17, No 1 (2013): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.1234/75

Abstract

Bt05 is marine bacterium which was isolated from the Jakarta Bay, Indonesia. The aim of this study was to characterize PAHs-degrading property, molecular identification by partial analysis of 16S rRNA gene and to partially analyze dioxygenase gene of Bt05 isolate. Our further study on this isolate revealed that it could degrade three PAHs (phenanthrene, dibenzothiophene, fluorene) between 60%–90% within 11 days at 100 ppm level. This finding indicated the potential of the isolate for bioremediation of PAHs. The isolate was identified as Alteromonas alvinellae by phylogenetic analysis of 16S rRNA gene sequence. Sequence analysis of the PCR product of PAH dioxygenase genes amplified using two primer set (iiDA and ppAH) of the isolate were identified 97% as naphthalene dioxygenase gene (phaAc) and 58% as 1,2-dioxygenase.
Alkane Degradation and Detection of Mono-xygenase Gene from Alcanivorax sp. from Jakarta Bay Thontowi, Ahmad; Yopi, Yopi
ANNALES BOGORIENSES Vol 15, No 2 (2011): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.1234/48

Abstract

Alkanes is a major component of crude oil that can be hydrolized by enzyme alkane monooxygenase from bacteria. Nine oil-degrading bacteria were analyzed their capability to degrade alkanes (pristane and paraffin). The result of growth test on paraffin and pristane were showed that 9 isolates could be devided into two groups. First group (BL09, BL31 and BL45) could degrade both paraffin and pristane, and second group (BL01, BL06, BL44, BL057, BL058 and BL071) preferred to degrade paraffin than pristane. Three isolates (BL09, BL31 and BL45) have activity to decrease paraffin and pristane until less 50% remain. Based on homology analysis of 16S rRNA gene sequences showed that isolates No. BL09, BL31 and BL45 were identified as Alcanivorax sp. and the partial sequences of the alkB gene from those three isolates are showing 66-68% of identity compare with some mono-oxygenase gen from database of genbank.Keywords: biodegradation, alkane, monooxygenase, cloning, alkB
Co-Authors - Yopi A.A. Ketut Agung Cahyawan W ADE ANDRIANI Atit Kanti BAMBANG SUNARKO Elvi Yetti, Elvi Endang Kusdiyantini Euis HERMIATI Faradila Ayu, Near Putri Hadi, Sofjan Hadi, Sofjan Kholida, Lutfi Nia Kusmiati Kusmiati Laksana, Raden Permana Budi Maemonah, Maemonah N Nurhayati NANIK RAHMANI Ni Nyoman Tri Puspaningsih Ni'mahtuzahroh, Ni'mahtuzahroh NUNIK SULISTINAH Nuzul Farini, Nuzul Oktaviani, Maulida Purkan Purkan, Purkan Putra, Filemon Jalu Nusantara Riksfardini Annisa ERMAWAR Safitriani, Safitriani Safitriani, Safitriani Siti Mardiana Sukma Nuswantara Suprihadi Suprihadi Suryani Suryani Susiana Purwantisari Takashi Watanabe Tanjung, Puspasari Noerwan Tanjung, Puspasari Noerwan Wijaya, Hans YOPI YOPI