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All Journal Jurnal Ilmu Ternak dan Veteriner Animal Production : Indonesian Journal of Animal Production
T Toharmat
Jurusan Ilmu Nutrisi dan Makanan Ternak Fakultas Peternakan, IPB
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Veterinary

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Ability of fiber bacteria isolated from buffalo rumen in digesting various sources of forage Prihantoro, Iwan; Toharmat, T; Evvyernie, D; ., Suryani; Abdullah, L
Indonesian Journal of Animal and Veterinary Sciences Vol 17, No 3 (2012)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1447.415 KB) | DOI: 10.14334/jitv.v17i3.699

Abstract

Local buffalo rumen fluid is a source of fiber digesting bacteria. Such bacteria presumably are well adapted to feed stuffs derived from agricultural byproducts with low quality. The purposes of this study were: (1) to isolate the fiber-digesting bacteria from buffalo rumen fluid, (2) to determine the fiber digesting characteristics and adaptability of such bacteria, and (3) to characterize the bacterial diversity.  Rumen fluids for the experiment were collected from buffaloes slaughtered at the Faculty of Animal Science, Bogor Agricultural University abattoir. Fiber-digesting bacteria were isolated using McDougall buffer supplemented with fibrous feed. A factorial experiment was conducted to study characteristics and adaptability of the bacteria using two factors: the type of bacteria and type of feed source of the fiber. Diversity among the bacteria was analyzed using the NTSys2.10 program.Results showed that nine bacterial isolates had a high adaptability to fiber feed based on CMCase. The highest CMCase activity bacteria for Pennisetum purpureum were A9 (11.36±1.70 unit/ml/h), A3 (11.22±0.60 unit/ml/h) and A42 (10.62±1.96 unit/ml/h). CMCase activity of fiber digesting bacteria from buffalo rumen fluid was not correlated with the number of bacteria in the culture. Based on genetic similarity, nine isolates were grouped into five types having similarity≥46%. Key Words: Buffalo Rumen Fluid, Fiber-Digesting Bacteria, Low Quality Forage
Potency of fiber rumen bacterial isolates from local buffalo inoculated into Frisian Holstein calves during preweaning period Prihantoro, Iwan; Evvyernie, D; ., Suryani; Abdullah, L; Yunitasari, N.S; Sari, A.P; Khairunisa, D; Haziq, A; Rahayu, N; Toharmat, T
Indonesian Journal of Animal and Veterinary Sciences Vol 17, No 4 (2012)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (220.773 KB) | DOI: 10.14334/jitv.v17i4.696

Abstract

Fiber-digesting bacteria are the main rumen bacteria that play an important role in digesting feed. These bacteria are adapted to low quality forage from agricultural byproduct. The aim of these study was to determine the potency of fiber-digesting bacteria consortium obtained from buffalo rumen inoculated to Frisian Holstein calves during preweaning on feed consumption, utilization, mineral uptake and physiological status. This study used 14 isolates of bacteria obtained from collection of Faculty of Animal Science, Bogor Agricultural University. The experimental unit consisted of six Frisian Holstein calves at two week old with the average body weight of 38.00 ± 6.23 kg. Calves were inoculated by 20 ml of fiber-digesting rumen bacterial isolates [4.56 x 109 cfu/ml] every morning for four weeks. Experimental design used was based on a completly randomized design with three calves received the respective inoculation (treatment group) and three calves without any inoculation (control group). Data were analyzed statistically using t-test method with α = 0.05 and 0.01. The results showed that fiber-digesting bacteria (FDB) from rumen buffalo have adapted in the calves rumen since preweaning periode. Inoculation FDB increased the number of rumen bacteria, digestibility of protein and P uptake calves at eight weeks old. Increased feed intake, uptake of Mg and cobalt calves at 14 weeks old. Without causing any negative effects on ADG, physiological status and rumen fermentability. Key Words: Fiber-Digesting Bacteria, Nutrient Intake, Rumen Fermentability
Casrea Engineering Based on Extruded-Cassava-Urea as Protein Supplement for Slow Release of Ammonia in the Rumen In Vitro Prasetiyono, BWHE; Suryahadi, Suryahadi; Toharmat, T; Syarief, R
ANIMAL PRODUCTION Vol 10, No 1 (2008): January
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (187.308 KB)

Abstract

CASREA is protein supplement (PS) based on extruded-cassava-urea as main materials.  The objective of this study was to examine the CASREA characteristics by evaluating ammonia concentration (NH3), Volatile fatty acids (VFA), rumen microbial protein synthesis (RMPS), and protein digestibility post rumen (PDPR). Variables of NH3, VFA and RMPS were analyzed with a completely randomized design in factorial (4X3), namely CASREA as factor A, consisted of: Casrea1 (made from 32% urea and 58% cassava without extrusion), Casrea2 (extrusion 22% urea and 68% cassava), Casrea3 (extrusion 27% urea and 63% cassava), and Casrea4 (extrusion 32% urea and 58% cassava), whereas the factor B was incubation times, consisted of  2, 4, and 6 hours incubation time. Variable of PDPR was analyzed with a completely randomized design, and the treatments were Casrea1, 2, 3, and 4. Results showed that there were no interactions between CASREA and incubation time on variables of NH3, VFA, and RMPS. Extrusion on CASREA materials reduced (P<0.05) NH3 and VFA, but increased (P<0.05) RMPS and PDPR.  The highest RMPS and PDPR were 29.04 mg and 76.16%, respectively and were attained by Casrea2.  Casrea1 had the highest NH3 (43.42 mM), whereas Casrea2 had the lowest (29.65 mM).  Meanwhile, NH3 caused by Casrea2, Casrea3, and Casrea4 was 29.65, 30.54 and 31.44 mM, respectively and the increase these values were not significantly different. The concentration of NH3, VFA, and RMPS were stable on 4 hours incubation time. The present findings suggest that CASREA made from extrusion of 22% urea and 68% cassava as main materials was PS for slow release of ammonia (SRA) in the rumen due to reduction of NH3, but it improved the utilization of nitrogen for rumen microbial protein synthesis.  (Animal Production 10(1): 34-41 (2008) Key Words: Extruded-cassava-urea, protein supplement, slow release of ammonia
Co-Authors A Haziq A.P Sari BWHE Prasetiyono, BWHE D Khairunisa Dwierra Evvyernie Iwan Prihantoro L Abdullah N Rahayu N.S Yunitasari R Syarief, R Suryahadi (Suryahadi) Suryani .