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All Journal JURNAL SAINS DAN TEKNOLOGI INDONESIA JURNAL TEKNOLOGI LINGKUNGAN Microbiology Indonesia AL KAUNIYAH BERITA BIOLOGI JURNAL ILMU KEFARMASIAN INDONESIA

Trismilah Trismilah

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Author-ID : 405847

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Education Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology

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PENINGKATAN PRODUKSI GAS HIDROGEN (H2 )DAN ETANOL PADA Bacillus pumilus DENGAN MUTASI MENGGUNAKAN Ethyl Methane Sulfonate (EMS) DAN SELEKSI DENGAN METODA PROTON SUICIDE Trismilah, Trismilah; AR, Mahyudin
BERITA BIOLOGI Vol 9, No 5 (2009)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Mutation by using Ethyl Methane Sulphonate (EMS) was carried out in the study of the enhancement of H2 and ethanol production in Bacillus pumilus.Target mutant was selected by using proton suicide method. Bacterial suspensions was spread into agar minimal U medium containing 13 or, 14, 15 and 16 u.1 of Ethyl Methane Sulphonate (EMS) and incubated at 37 C for 3, or 4 , or 5, or 6 hours.The method of proton suicide was applied by the addition of equimolar of 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 75, 100, 125, 150, 175, 180, 185, 190, 195, 200 mM NaBr and NaBrO3.Triphenyl tetra chloride (TTC) was also added as indicator into agar plate in order to distinguish between wild type and mutants. Fermentation was carried out using glycerol complex medium.Hydrogen gas(H,) contain was determined by the replacement of NaCI solution in cylindrical glass and the ethanol was measured by gas chromatography. After mutation, several mutants were observed. In Mutant ( Asp8) which was obtained by treatment of 195 equimolar of NaBr and NaBrO,, production of ethanol and H2 were higher 10 fold and 1.13 fold, respectively compare to the wild type while acids production decreased. The data indicated that mutation might provoke metabolic alteration especially in acid production.

PELEPASAN TINTA PADA KERTAS NCR (NO CARBON REQUIRED) BEKAS DAN KERTAS UANG MENGGUNAKAN XILANASE Trismilah, Trismilah
Jurnal Sains dan Teknologi Indonesia Vol. 13 No. 3 (2011)
Publisher : Badan Pengkajian dan Penerapan Teknologi

Recycling paper is a solution to overcome the depletion of virgin pulp from trees and reduce the impact of global warming. Deinking paper is very important in the process of recycling paper. Xylanase application of research required for deinking outworn NCR paper and paper money. Deinking enzymatic is a clean technology and produces less waste than conventional deinking (chemicals). Xylanase AQ-1 which is a crude enzyme from Bacillus sp isolates AQ-1 can work well in the deinking process on the amount of enzyme addition of 0.2% in the pulp with the conditions of pH 7 and temperature of 50oC for 120 minutes. While the recombinant xylanase AQ-1 which is the result of genetic xylanase AQ-1 grown in E. coli can work well in the deinking process on the amount of enzyme addition of 0.1% in the pulp with the conditions of pH 7 and temperature of 50oC for 60 minutes. Xylanase AQ-1 increases the degree of whiteness of green NCR paper pulp from the control (40.1) into (53.9) and pulp paper moneyof the control (50.1) into (66.6). While AQ-1 recombinant xylanase increases the degree of whiteness of green NCR paper pulp from control ( 40.1) into (52.0) pulp and paper money from the control (50.1) into (63.4). Xylanase AQ-1 and recombinant xylanase AQ-1 potential in the green NCR paper deinking and paper deinking paper money, although the money still leaves some smudges on the results because of a special polymer coating that coats the waterproof paper money proficiency level.

MEMBRAN POLYETHERSULFONE DAN REGENERATED CELLULOSE UNTUK ULTRAFILTRASI Trismilah, Trismilah; Lutfi, Achmadin
Jurnal Sains dan Teknologi Indonesia Vol. 11 No. 2 (2009)
Publisher : Badan Pengkajian dan Penerapan Teknologi

Purification of xilanase result of fermentation from Bacillus stearothermophilus DSM 22 using membrane polyethersulfone and regenerated cellulose, each measuring 30 kD. Variations in pH 4.94, 5.80, 6.60, 7.40, 8.20. Analysis of enzyme activity, protein content and enzyme specific activity carried out on permeate and retentate. This study aimed to learn the best pH condition and the appropriate type of membrane process in the purification method xilanase with ultrafiltrasi. Research of resultsindicate that pH is very influential in the process ultrafiltration xilanase, each membrane has a different characteristic. Purification xilanase best use of ultrafiltration membrane polyethersulfone achieved in the pH 4.94 with a specific activity 13,888 U / mg in the permeate. Purification xilanase best ultrafiltration use of regenerated cellulose membrane at pH 8.20 achieved with specific activity 12397 U / mg in the retentate.

PEMANFAATAN BERBAGAI JENIS PATI SEBAGAI SUMBER KARBON UNTUK PRODUKSI a-AMILASE EKSTRASELULER Bacillus sp SW2 Trismilah, Trismilah; Wahyuntari, Budiasih
Jurnal Sains dan Teknologi Indonesia Vol. 11 No. 3 (2009)
Publisher : Badan Pengkajian dan Penerapan Teknologi

Currently enzymes become a need of food and non-food industries. Alpha amylase (a-1,4 glucanohydrolase, EC 3.2.1.1) is an enzyme that hydrolyses starch into oligosaccharides and dextrin. The enzyme has been commercially available which mostly produced by Bacillus spp. The bacterium used in thisexperiment was Bacillus sp SW2, which was isolated from Composting Unit at Bumi Serpong Damai, Tangerang. The aim of this experiment was to find the most appropriate starch as an carbon source for enzyme production. The starches observed were tapioca, potato, and cornstarch at concentration of5%. The fermentation was conducted in shaking incubator, in 125 ml Erlenmeyer at 60°C, various pHs, and agitations. The pHs observed were 6, 7.5, 8 and 9 while the rates of agitation applied were 150, 200, and 250 rpm. The results showed that the highest enzyme activity was 20.99 Unit/ml, whichwas reached after 42 hours of fermentation using cornstarch, pH 8 and 200 rpm agitation.

PRODUKSI XILANASE MENGGUNAKAN MEDIA LIMBAH PERTANIAN DAN PERKEBUNAN Trismilah, Trismilah; Waltam, Deden Rosid
Jurnal Teknologi Lingkungan Vol. 10 No. 2 (2009)
Publisher : Center for Environmental Technology - Agency for Assessment and Application of Technology

Waste Agriculture of paddy like hay, bran, chaff and frond of banana and alsowaste plantation of coconut root and cangkang of sawit pregnant [of] lignoselulosa(cellulose, lignine and hemiselulosa).In order to searching alternative materials for the production of xylanase hencedone by research of xylanase production of agriculture waste and plantationwaste like the above. Xylanase produced from Bacillus stearothermopillusDSM 22, using hay, bran, chaff and frond of banana and also coconut root andcangkang of sawit as source of carbon while as source of nitrogen and nutrisi bymolasses and urea. Fermentation done in the erlenmeyer use incubator shakerwith condition of temperature 550C, pH early 8, and agitation 250 rpm. Fromresult of research obtained activity of xylanase highest 0.523 Unit / ml.menit and0.429 Unit / ml.menit at to 30 hours with bran media of oven ( DO) and naturalhay ( JA) respectively with natural bran medium (DA) activity of xylanase highest0.514 Unit / ml.menit at 24 hours fermentation. Fermentation using fermentor withnatural hay and condition of temperature 550C, pH 8, and agitation 250 rpm resultactivity of xylanase highest 2.47 Unit / ml.menit at to 32 hours.

OPTIMASI DAN PEMEKATAN LIPASE Bacillus halodurans CM1 Aisyah, Arina; Mangunwardoyo, Wibowo; Trismilah, Trismilah; Suhendar, Dadang
Al-Kauniyah: Jurnal Biologi Vol 10, No 2 (2017): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Abstrak Lipase diketahui memiliki peranan penting dalam bidang industri. Produksi lipase dapat dihasilkan oleh kapang, khamir, dan bakteri. Penelitian bertujuan untuk meningkatkan aktivitas lipase yang dihasilkan oleh Bacillus halodurans CM1. Aktivitas lipase dapat ditingkatkan dengan optimasi komposisi media, mutasi bakteri dengan radiasi gamma dan N-methyl-Nâ€™-nitro-N-nitrosoguanidine (NTG). Enzim yang dihasilkan dipekatkan dengan metode stirred-cell ultrafiltration (UF)-ammonium sulfat dan UF-Polyethylene glycol (PEG). Uji aktivitas dilakukan pada tujuh media yang berbeda untuk mendapatkan media produksi. Delapan variabel komposisi media dioptimasi dengan rancangan Plackett-Burman. Bakteri dimutasi dengan radiasi gamma dosis 0,1â€“0,4 kGy dan NTG 0,05â€“0,15 mg/mL dengan waktu inkubasi 1â€“3 jam. Hasil penelitian menunjukkan bahwa media produksi yang digunakan berdasarkan optimasi media dan komposisi media Plackett-Burman adalah media dasar Bora & Bora yang mengandung 0,5% palm oil (PO) dan 0,09% CaCl2. Aktivitas lipase optimal diproduksi oleh bakteri hasil mutasi dengan NTG 0,1 mg/mL yang diinkubasi selama 3 jam. Pemekatan enzim UF-ammonium sulfat dan UF-PEG mampu meningkatkan aktivitas enzim lipase sebesar 18,44%.Â Â Abstract Lipase is known to have an important role in the industrial field. Lipase can be produced by molds, yeasts, and bacteria. The research aimed to increase the activity of lipase produced by Bacillus halodurans CM1. Lipase activity can be improved by optimization of the composition of the media, the mutation of bacteria with gamma radiation and N-methyl-N'-nitro-N-nitrosoguanidine (NTG). The enzyme was concentrated by stirred-cell ultrafiltration method (UF)-ammonium sulfate and UF-Polyethylene glycol (PEG). The activity test was performed on seven different media to get production media. The eight variables of the media composition were optimized by Plackett-Burman design. The bacteria were subject to mutation by using 0.1â€“0.4 kGy dose of gamma radiation and 0.05â€“0.15 mg/mL NTG with incubation time for 1â€“3 hours. The results showed that the production media used based on optimization and composition of Plackett-Burman media was Bora Bora medium that containing 0.5% palm oil (PO) and 0.09% CaCl2. Optimum lipase activity was produced by the bacterium that mutated with 0.1 mg/mL NTG, incubated for 3 hours. The concentrated by UF-ammonium sulfate and UF-PEG could increase the lipase activity by 18.44%.

The Effect of Growth Medium pH towards Trypsin-Like Activity Produced by Lactic Acid Bacteria DYAH WULANSARI; BUDIASIH WAHYUNTARI; TRISMILAH TRISMILAH; ASTUTIATI NURHASANAH
Microbiology Indonesia Vol. 6 No. 2 (2012): June 2012
Publisher : Indonesian Society for microbiology

In cases of pancreatic disease, trypsin deficiency often occurs due to reduced expression of trypsin in the pancreas. Patients with pancreatic problem can be treated with a supplement containing digestive enzymes, including trypsin. However, most of the enzymes currently used for the treatment are derived from porcine and bovine sources. On the other hand, lactic acid bacteria are also known to show trypsin-like activity. In the previous work, our group screened 11 lactic acid bacteria isolates, which had previously been proven to show serine protease activity, for trypsin-like activity. The strains were initially grown in MRS (de Mann, Rogosa and Sharpe) medium before being transferred directly to the production medium to produce trypsin. During the previous study, the initial pH of the production medium was set at 6 (the same as the MRS medium pH), which is the optimum pH for the cell growth of lactic acid bacteria. However, most trypsin has an optimum pH of around 8. In this study, we altered the production medium pH to 8 and we harvested the lactic acid bacteria from MRS medium by centrifugation prior to their inoculation to the production medium. Observation of the culture growth and enzyme activity indicated that the new strategy improved the enzyme activity expressed by some strains.

Pengaruh Kadar Nitrogen dalam Media pada Pembuatan Protease Menggunakan Bacillus megaterium DSM 319 Trismilah Trismilah; Sumaryanto Sumaryanto
JURNAL ILMU KEFARMASIAN INDONESIA Vol 3 No 1 (2005): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

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The media components and fermentation conditions play an important role for the result of fermentation processes, for instance nitrogen is one of the element which is influence for the growth of microorganism. Therefore it is needed the available media formulation for the optimal growth of microorganism with high productivity. It was shown in this protease production experiment by using Bacillus megaterium DSM 319 with media of Geolitti Cantoni (G&C) with modified of urea as source of nitrogen. The variation of carbon and nitrogen ratio which are used in this process are 3 : 0.8, 3: 1.5,3 :2,3: 2.5. Fermentation conditions were adjusted : temperature at 37°C, pH 7.5 and agitation of 250 rpm. The result of this process indicated that carbon and nitrogen ratio of 3 : 2 giving highest enzyme activity at 18 hours. The other results such are specific activity 6.552 Unit / protein mg, specific growth rate (u max/h) 0.074, duplication time (t jam) 9.367, coeffisient convert (Yx/s) 0.23.

Co-Authors Achmadin Lutfi Aisyah, Arina AR, Mahyudin ASTUTIATI NURHASANAH Budiasih Wahyuntari Budiasih Wahyuntari deden rosid waltam DYAH WULANSARI Suhendar, Dadang Sumaryanto Sumaryanto WIBOWO MANGUNWARDOYO

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